10 results on '"Gatti, Monica"'
Search Results
2. Adiponectin as Novel Regulator of Cell Proliferation in Human Glioblastoma.
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Porcile, Carola, Di Zazzo, Erika, Monaco, Maria Ludovica, D'Angelo, Giorgia, Passarella, Daniela, Russo, Claudio, Di Costanzo, Alfonso, Pattarozzi, Alessandra, Gatti, Monica, Bajetto, Adriana, Zona, Gianluigi, Barbieri, Federica, Oriani, Giovannangelo, Moncharmont, Bruno, Florio, Tullio, and Daniele, Aurora
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ADIPONECTIN ,CELL proliferation ,GLIOBLASTOMA multiforme ,CANCER invasiveness ,TUMOR growth ,CELL cycle - Abstract
Adiponectin (Acrp30) is an adipocyte-secreted hormone with pleiotropic metabolic effects, whose reduced levels were related to development and progression of several malignancies. We looked at the presence of Acrp30 receptors in human glioblastomas (GBM), hypothesizing a role for Acrp30 also in this untreatable cancer. Here we demonstrate that human GBM express Acrp30 receptors (AdipoR1 and AdipoR2), which are often co-expressed in GBM samples (70% of the analyzed tumors). To investigate the effects of Acrp30 on GBM growth, we used human GBM cell lines U87-MG and U251, expressing both AdipoR1 and AdipoR2 receptors. In these cells, Acrp30 treatment inhibits DNA synthesis and cell proliferation rate, inducing arrest in G1 phase of the cell cycle. These effects were correlated to a sustained activation of ERK1/2 and Akt kinases, upon Acrp30 treatment. Our results suggest that Acrp30 may represent a novel endogenous negative regulator of GBM cell proliferation, to be evaluated for the possible development of novel pharmacological approaches. J. Cell. Physiol. 229: 1444-1454, 2014. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
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- 2014
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3. Investigation of the ability of dairy nonstarter lactic acid bacteria to grow using cell lysates of other lactic acid bacteria as the exclusive source of nutrients.
- Author
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Sgarbi, Elisa, Bottari, Benedetta, Gatti, Monica, and Neviani, Erasmo
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FUNGUS-bacterium relationships ,PROKARYOTES ,LACTIC acid ,PLANT nutrients ,POLYCARBONATES - Abstract
A new device named a cell sonicated substrate membrane system ( CSSMS), consisting of a tissue culture insert that supplies nutrients, in the form of starter lactic acid bacteria ( SLAB) lysate extract, through a polycarbonate membrane which isolates it from the nonstarter lactic acid bacteria ( NSLAB) inoculum and serves as a support for bacterial growth, was developed. The CSSMS was used to demonstrate that the Lactobacillus helveticus SLAB lysate provides sufficient nutrients to allow the growth of L. casei and L. rhamnosus NSLAB in vitro. The results support the hypothesis that NSLAB could grow in aged cheese using only SLAB cell lysate as nutrients. [ABSTRACT FROM AUTHOR]
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- 2014
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4. Growth promotion of Bifidobacterium and Lactobacillus species by proteinaceous hydrolysates derived from poultry processing leftovers.
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Lazzi, Camilla, Meli, Federica, Lambertini, Francesca, Bottesini, Chiara, Nikolaev, Ilya, Gatti, Monica, Sforza, Stefano, Koroleva, Olga, Popov, Vladimir, Neviani, Erasmo, and Dossena, Arnaldo
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BIFIDOBACTERIUM ,LACTOBACILLUS ,POULTRY processing plants ,BACTERIAL growth ,MOLECULAR weights ,BIOMASS - Abstract
Bacterial growth media represent a high cost in industrial applications, and for this reason, it is economically important to find less expensive supplements to replace the traditional ones. In the present work, peptide hydrolysates obtained from poultry meat and bone residues (functional animal protein [FAP]) and from feathers (functional feather protein [FFP]) were studied to determine their ability for the production of microbial biomass with improved viability. The results obtained were compared with those obtained with other supplement nutritive compounds used in fermentation growth media. The molecular composition of the hydrolysates in terms of total and soluble nitrogen, molecular weight distribution, total and free amino acids, was determined. The growth and cellular state of Bifidobacterium and Lactobacillus strains were studied by turbidimetric measurements and direct count by fluorescence microscopy. Overall, this study suggested that by-products from poultry industry provide a good alternative to substitute expensive supplements for growth of Lactobacillus and Bifidobacterium with a high level of viability [ABSTRACT FROM AUTHOR]
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- 2013
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5. Peptide Receptor Targeting in Cancer: The Somatostatin Paradigm.
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Barbieri, Federica, Bajetto, Adriana, Pattarozzi, Alessandra, Gatti, Monica, Würth, Roberto, Thellung, Stefano, Corsaro, Alessandro, Villa, Valentina, Nizzari, Mario, and Florio, Tullio
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PEPTIDE receptors ,TARGETED drug delivery ,CANCER treatment ,SOMATOSTATIN ,PATHOLOGICAL physiology ,NEUROPEPTIDES ,THERAPEUTICS - Abstract
Peptide receptors involved in pathophysiological processes represent promising therapeutic targets. Neuropeptide somatostatin (SST) is produced by specialized cells in a large number of human organs and tissues. SST primarily acts as inhibitor of endocrine and exocrine secretion via the activation of five G-protein-coupled receptors, named sstl-5, while in central nervous system, SST acts as a neurotransmitter/neuromodulator, regulating locomotory and cognitive functions. Critical points of SST/SST receptor biology, such as signaling pathways of individual receptor subtypes, homo- and heterodimerization, trafficking, and cross-talk with growth factor receptors, have been extensively studied, although functions associated with several pathological conditions, including cancer, are still not completely unraveled. Importantly, SST exerts antiproliferative and antiangiogenic effects on cancer cells in vitro, and on experimental tumors in vivo. Moreover, SST agonists are clinically effective as antitumor agents for pituitary adenomas and gastro-pancreatic neuroendocrine tumors. However, SST receptors being expressed by tumor cells of various tumor histotypes, their pharmacological use is potentially extendible to other cancer types, although to date no significant results have been obtained. In this paper the most recent findings on the expression and functional roles of SST and SST receptors in tumor cells are discussed. [ABSTRACT FROM AUTHOR]
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- 2013
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6. Metabolic and proteomic adaptation of Lactobacillus rhamnosus strains during growth under cheese-like environmental conditions compared to de Man, Rogosa, and Sharpe medium.
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Bove, Claudio Giorgio, Angelis, Maria De, Gatti, Monica, Calasso, Maria, Neviani, Erasmo, and Gobbetti, Marco
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- 2012
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7. Balance between somatostatin and D2 receptor expression drives TSH-secreting adenoma response to somatostatin analogues and dopastatins.
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Gatto, Federico, Barbieri, Federica, Gatti, Monica, Wurth, Roberto, Schulz, Stefan, Ravetti, Jean-Louis, Zona, Gianluigi, Culler, Michael D., Saveanu, Alexandru, Giusti, Massimo, Minuto, Francesco, Hofland, Leo J., Ferone, Diego, and Florio, Tullio
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SOMATOSTATIN ,NEUROSURGERY ,CELL proliferation ,TACHYPHYLAXIS ,TUMOR treatment - Abstract
Summary Context First-line therapy for thyrotropin-secreting pituitary adenomas (TSHomas) is neurosurgery, while medical treatment rests mainly on somatostatin analogues. Clinically available sst
2 -preferring analogues, octreotide and lanreotide, induce normalization of hormone levels in approximately 90% of patients and tumour shrinkage in 45%. Objective We evaluated somatostatin 1, 2, 3 and 5 and dopamine D2 receptor expression in tumour samples from three TSHomas, and the relationships between receptor expression, in vitro antiproliferative response and clinical data, including octreotide test and three months of therapy with octreotide long-acting repeatable (LAR). TSHoma cell proliferation was tested in vitro using octreotide, cabergoline and two chimeric compounds, BIM-23A760 and BIM-23A387. Results All patients showed significant TSH lowering to acute octreotide test, but a hormonal response to long-term treatment was observed in only two patients, showing a high sst5 /sst2 ratio. Patient 2, characterized by high expression of sst2 and sst1 and a relative lower expression of sst5 , experienced tachyphylaxis after prolonged octreotide treatment. In vitro, the somatostatin/dopamine receptor agonist BIM-23A760 caused the highest antiproliferative effect among those tested. Combined treatment with octreotide and cabergoline displayed an additive effect of magnitude comparable to that of the other chimeric compound (BIM-23A387). Octreotide resistance was confirmed in cells isolated from the nonresponder patient, although it could be overcome by treatment with the chimeric compounds. Conclusions A high sst5 /sst2 ratio might be predictive of a positive outcome to long-term treatment with somatostatin analogues in TSHomas. Moreover, combined somatostatin and D2 receptor targeting might be considered as a potential tool to improve the response rate in octreotide-resistant tumours. [ABSTRACT FROM AUTHOR]- Published
- 2012
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8. An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells.
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Castelnuovo, Manuele, Massone, Sara, Tasso, Roberta, Fiorino, Gloria, Gatti, Monica, Robello, Mauro, Gatta, Elena, Berger, Audrey, Strub, Katharina, Florio, Tullio, Dieci, Giorgio, Cancedda, Ranieri, and Pagano, Aldo
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Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that the synthesis of a pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes, and a dramatic reduction of the tumor initiating cell (TIC) fraction in the NB cell population. Notably, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Altogether, these results suggest the induction of NDM29 expression as possible treatment to increase cancer cells vulnerability to therapeutics and the measure of its synthesis in NB explants as prognostic factor of this cancer type. [ABSTRACT FROM AUTHOR]
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- 2010
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9. SDF-1 Controls Pituitary Cell Proliferation through the Activation of ERK1/2 and the Ca2+-Dependent, Cytosolic Tyrosine Kinase Pyk2.
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MASSA, ALESSANDRO, CASAGRANDE, SILVIA, BAJETTO, ADRIANA, PORCILE, CAROLA, BARBIERI, FEDERICA, THELLUNG, STEFANO, ARENA, SARA, PATTAROZZI, ALESSANDRA, GATTI, MONICA, CORSARO, ALESSANDRO, ROBELLO, MAURO, SCHETTINI, GENNARO, and FLORIO, TULLIO
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CHEMOKINES ,CELL proliferation ,MITOGEN-activated protein kinases ,PITUITARY gland ,G proteins ,PROTEIN-tyrosine kinases ,MEMBRANE proteins - Abstract
Stromal cell-derived factor-1 (SDF-1) is a chemokine of the CXC subfamily that exerts its effects via CXCR4, a G-protein-coupled receptor. CXCR4 is often expressed by tumor cells, and its activation causes tumor cell proliferation. Using GH4C1 cells, here we show that SDF-1 induced cell proliferation in a dose-dependent manner. Thus, we evaluated the intracellular signaling involved in this effect. SDF-1 increased cytosolic [Ca
2+ ] and activated Pyk2, ERK1/2, and BKCa channels. To correlate these intracellular effectors with the proliferative activity of SDF-1, we inhibited their activity using BAPTA-AM (Ca2+ chelator), PD98059 (MEK inhibitor), salicylate (Pyk2 inhibitor), and TEA (K+ channel blocker). All these compounds reverted SDF-1-induced proliferation, suggesting the involvement of multiple intracellular pathways. To identify a possible crosstalk and a molecular ordering among these pathways, we tested these antagonists on SDF-1-dependent activation of ERK1/2, Pyk2, and BKCa channels. We report that the inhibition of [Ca2+ ]i increase or the blockade of BKCa channel activity did not affect ERK1/2 activation by SDF-1; Pyk2 activation was purely Ca2+ -dependent, not involving ERK1/2 or BKCa channels; and BKCa channel activity was antagonized by Pyk2 but not by ERK1/2 inhibitors. These data suggest that SDF-1-dependent increase of [Ca2+ ]i activates Pyk2, which, in turn, regulates BKCa channel activity. Conversely, ERK1/2 activation is an independent phenomenon. In conclusion, we demonstrate that SDF-1 induces proliferation of GH4C1 cells, suggesting that the activation of CXCR4 may represent a novel regulatory mechanism for pituitary cell proliferation which may contribute to pituitary adenoma development. [ABSTRACT FROM AUTHOR]- Published
- 2006
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10. A comparative study of stress and burnout among staff caregivers in nursing homes and acute geriatric wards.
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Cocco, Ennio, Gatti, Monica, De Mendonça Lima, Carlos Augusto, and Camus, Vincent
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PSYCHOLOGICAL burnout , *CAREGIVERS , *HOSPITAL wards , *NURSING care facilities , *PSYCHOLOGICAL stress - Abstract
Objective To compare levels of stress and burnout among staff caregivers in nursing homes and acute geriatric wards of general hospitals.Methods A cross-sectional survey was conducted in three nursing homes (total of 522 beds, 270 caregivers) and nine geriatric sections of general hospitals (total of 371 beds, 280 caregivers). Staff caregivers were asked to answer a four-part questionnaire made up of socio-demographic data, the General Health Questionnaire (GHQ-12), the Maslach Burnout Inventory (MBI) and the Stressful Events Questionnaire (SEQ).Results 355 carers (172 from nursing homes, 183 from acute geriatric wards) answered the questionnaire (response rate 66%). Bivariate analysis reveals that general hospital carers show higher GHQ scores, higher MBI—Depersonalisation (DP) and Emotional Exhaustion (EE) sub-scores and lower MBI—Personal Accomplishment sub-scores. Stressful Events (as revealed by the SEQ) are more frequently reported by general hospital carers, particularly events related to patients' behavioural disorders. Multivariate analysis shows that general hospital work-setting, professional role, female gender and patient/carer ratio are significant explanatory variables of a high MBI—EE sub-score, while general work setting and disability are the best explanatory variables of a high MBI—DP sub-score. Professional role and general hospital work-setting are independent factors in a low MBI—Personal Accomplishment (PA) sub-score.Conclusion These results appear to show that levels of stress and burnout among staff caregivers are moderate in acute geriatric wards, but significantly higher than in nursing homes. This suggests that increasing the rate of trained staff and improving staff support—for instance by the implementation of Consultation-Liaison (C-L) Psychiatry and/or continuing education programmes—could be needed mostly in acute geriatric wards. Copyright © 2002 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]- Published
- 2003
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