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Many plant pathogens secrete effector proteins into the host plant to suppress host immunity and facilitate pathogen colonization. The necrotrophic pathogen Sclerotinia sclerotiorum causes severe plant diseases and results in enormous economic losses, in which secreted proteins play a crucial role. SsCVNH was previously reported as a secreted protein, and its expression is significantly upregulated at 3 h after inoculation on the host plant. Here, we further demonstrated that deletion of SsCVNH leads to attenuated virulence. Heterologous expression of SsCVNH in Arabidopsis enhanced pathogen infection, inhibited the host PAMP‐triggered immunity (PTI) response and increased plant susceptibility to S. sclerotiorum. SsCVNH interacted with class III peroxidase AtPRX71, a positive regulator of innate immunity against plant pathogens. SsCVNH could also interact with other class III peroxidases, thus reducing peroxidase activity and suppressing plant immunity. Our results reveal a new infection strategy employed by S. sclerotiorum in which the fungus suppresses the function of class III peroxidases, the major component of PTI to promote its own infection. [ABSTRACT FROM AUTHOR]

Apoptosis‐like programmed cell death is associated with fungal development, ageing, pathogenicity and stress responses. Here, to explore the potential of Botrytis cinerea type II inhibitor of apoptosis (IAP) BcBIR1 in elevating the biocontrol efficacy of Coniothyrium minitans, the BcBIR1 gene was heterologously expressed in C. minitans. Results indicated that the strains expressing BcBIR1 had higher rates of conidiation, mycelial growth and biomass growth than the wild‐type strain. Moreover, BcBIR1 was found to inhibit apoptosis, indicating its role as an IAP in C. minitans. Under various abiotic stresses, the growth rates of BcBIR1‐expressing strains were significantly higher than that of the wild‐type strain. Moreover, the conidial survival rate of the BcBIR1‐expressing strains treated with ultraviolet irradiation was enhanced. In antifungal activity assay, the culture filtrates of BcBIR1‐expressing strains displayed a stronger inhibitory effect on B. cinerea and Sclerotinia sclerotiorum than the wild‐type strain. The study also found that BcBIR1 expression increased the mycoparasitism against the sclerotia, but not the hyphae of S. sclerotiorum. Taken together, these results suggest that BcBIR1 enhances vegetative growth, conidiation, anti‐apoptosis activity, abiotic stress resistance, antifungal activity and mycoparasitism in C. minitans. As an IAP, BcBIR1 may improve the control capacity of C. minitans against S. sclerotiorum. [ABSTRACT FROM AUTHOR]

Hydrogels have been widely used in wound dressings owing to their biocompatibility and bacteriostatic properties. In this study, a chitosan‐citric acid gel film was prepared using chitosan as the raw material and citric acid as the acid solvent and cross‐linking agent. In addition, to increase the antibacterial effect of the gel film, ketoconazole/β‐cyclodextrin inclusion complex was prepared by inclusion technology and added to the gel film to prepare a chitosan‐citric acid sustained‐release gel film loaded with ketoconazole/β‐cyclodextrin inclusion complex for treating cutaneous candidiasis. The physical and chemical properties of the chitosan‐citric acid gel films were investigated using infrared spectroscopy, x‐ray diffraction, water vapor transmittance test, thermal analysis, and swelling tests. Drug release, antibacterial, and toxicity tests of the chitosan‐citric acid gel film loading with ketoconazole/β‐cyclodextrin inclusion complex were carried out in vitro. The composite film showed good sustained drug release behavior, antibacterial effects, and biocompatibility in vitro. These results support that the chitosan‐citric acid hydrogel has great potential as a gel wound dressing for the treatment of cutaneous candidiasis. [ABSTRACT FROM AUTHOR]

Co‐cured damping composite structures have broad application prospects in high‐tech fields such as aerospace and space vehicles because of their low weight, large damping, and sufficient stiffness. In order to improve dynamic properties of conventional composite structures, a new type of damping composites embedded with multilayer viscoelastic film is proposed in this paper. Based on the complex modulus theory and vibration mechanics of composite plates and shells, vibration differential equation of composite plates with multilayer viscoelastic film is derived. The constitutive relation of the complex structure is established. The consistency of theoretical derivation and numerical simulation results provides a reference for the optimization of dynamic performance of damping sandwich composites. The effects of the distribution of viscoelastic film and the geometric structure parameters on dynamic performance of co‐cured damping composites are also discussed. The results show that the embedding of multi‐layer viscoelastic film can greatly enhance the loss factors of co‐cured composite structures under same damping layer thickness. This work lays a foundation for optimization of dynamic performance of lightweight and large damping composite structures. [ABSTRACT FROM AUTHOR]

Summary: Plants perceive pathogens and induce robust transcriptional reprogramming to rapidly achieve immunity. The mechanisms of how immune‐related genes are transcriptionally regulated remain largely unknown.Previously, the trihelix transcriptional factor ARABIDOPSIS SH4‐RELATED 3 (ASR3) was shown to negatively regulate pattern‐triggered immunity (PTI) in Arabidopsis thaliana. Here, we identified another trihelix family member ASR3‐Interacting Transcriptional Factor 1 (AITF1) as an interacting protein of ASR3.ASR3‐Interacting Transcriptional Factor 1 and ASR3 form heterogenous and homogenous dimers in planta. Both aitf1 and asr3 single mutants exhibited increased resistance against the bacterial pathogen Pseudomonas syringae, but the double mutant showed reduced resistance, suggesting AITF1 and ASR3 interdependently regulate immune gene expression and resistance. Overexpression of AITF1 triggered autoimmunity dependently on its DNA‐binding ability and the presence of ASR3. Notably, autoimmunity caused by overexpression of AITF1 was dependent on a TIR‐NBS‐LRR (TNL) protein suppressor of AITF1‐induced autoimmunity 1 (SAA1), as well as enhanced disease susceptibility 1 (EDS1), the central regulator of TNL signaling. ASR3‐Interacting Transcriptional Factor 1 and ASR3 directly activated SAA1 expression through binding to the GT‐boxes in SAA1 promoter.Collectively, our results revealed a mechanism of trihelix transcription factor complex in regulating immune gene expression, thereby modulating plant disease resistance and autoimmunity. [ABSTRACT FROM AUTHOR]

Shadow removal from a single image is an ill‐posed problem because shadow generation is affected by the complex interactions of geometry, albedo, and illumination. Most recent deep learning‐based methods try to directly estimate the mapping between the non‐shadow and shadow image pairs to predict the shadow‐free image. However, they are not very effective for shadow images with complex shadows or messy backgrounds. In this paper, we propose a novel end‐to‐end depth‐aware shadow removal method without using depth images, which estimates depth information from RGB images and leverages the depth feature as guidance to enhance shadow removal and refinement. The proposed framework consists of three components, including depth prediction, shadow removal, and boundary refinement. First, the depth prediction module is used to predict the corresponding depth map of the input shadow image. Then, we propose a new generative adversarial network (GAN) method integrated with depth information to remove shadows in the RGB image. Finally, we propose an effective boundary refinement framework to alleviate the artifact around boundaries after shadow removal by depth cues. We conduct experiments on several public datasets and real‐world shadow images. The experimental results demonstrate the efficiency of the proposed method and superior performance against state‐of‐the‐art methods. [ABSTRACT FROM AUTHOR]

During a review of figures for this article, the authors have discovered inclusion of inadvertent duplications and incorrect placement of images. The large size of these images and the high similarity among them may also cause an error in capturing images. A novel antisense long non-coding RNA participates in asexual and sexual reproduction by regulating the expression of GzmetE in Fusarium graminearum. [Extracted from the article]

Long noncoding RNAs (lncRNAs) are crucial regulators of gene expression in many biological processes, but their biological functions remain largely unknown, especially in fungi. Fusarium graminearum is an important pathogen that causes the destructive disease Fusarium head blight (FHB) or head scab disease on wheat and barley. In our previous RNA sequencing (RNA‐Seq) study, we discovered that lncRsp1 is an lncRNA that is located +99 bp upstream of a putative sugar transporter gene, Fgsp1, with the same transcription direction. Functional studies revealed that ΔlncRsp1 and ΔFgsp1 were normal in growth and conidiation but had defects in ascospore discharge and virulence on wheat coleoptiles. Moreover, lncRsp1 and Fgsp1 were shown to negatively regulate the expression of several deoxynivalenol (DON) biosynthesis genes, TRI4, TRI5, TRI6, and TRI13, as well as DON production. Further analysis showed that the overexpression of lncRsp1 enhanced the ability of ascospore release and increased the mRNA expression level of the Fgsp1 gene, while lncRsp1‐silenced strains reduced ascospore discharge and inhibited Fgsp1 expression during the sexual reproduction stage. In addition, the lncRsp1 complementary strains lncRsp1‐LC‐1 and lncRsp1‐LC‐2 restored ascospore discharge to the level of the wild‐type strain PH‐1. Taken together, our results reveal the distinct and specific functions of lncRsp1 and Fgsp1 in F. graminearum and principally demonstrate that lncRsp1 can affect the release of ascospores by regulating the expression of Fgsp1. [ABSTRACT FROM AUTHOR]

Sclerotinia sclerotiorum and Botrytis cinerea are typical necrotrophic pathogens that can attack more than 700 and 3000 plant species, respectively, and cause huge economic losses across numerous crops. In particular, the absence of resistant cultivars makes the stem rot because of S. sclerotiorum the major threat of rapeseed (Brassica napus) worldwide along with Botrytis. Previously, we identified an effector‐like protein (SsSSVP1) from S. sclerotiorum and a homologue of SsSSVP1 on B. cinerea genome and found that SsSSVP1 could interact with BnQCR8 of rapeseed, a subunit of the cytochrome b‐c1 complex. In this study, we found that BnQCR8 has eight homologous copies in rapeseed cultivar Westar and reduced the copy number of BnQCR8 using CRISPR/Cas9 to improve rapeseed resistance against S. sclerotiorum. Mutants with one or more copies of BnQCR8 edited showed strong resistance against S. sclerotiorum and B. cinerea. BnQCR8‐edited mutants did not show significant difference from Westar in terms of respiration and agronomic traits tested, including the plant shape, flowering time, silique size, seed number, thousand seed weight and seed oil content. These traits make it possible to use these mutants directly for commercial production. Our study highlights a common gene for breeding of rapeseed to unravel the key hindrance of rapeseed production caused by S. sclerotiorum and B. cinerea. In contrast to previously established methodologies, our findings provide a novel strategy to develop crops with high resistance against multiple pathogens by editing only a single gene that encodes the common target of pathogen effectors. [ABSTRACT FROM AUTHOR]

Huperzia serrata (H. serrata) produces various types of effective lycopodium alkaloids, especially Huperzine A (HupA), which is a promising drug for the treatment of Alzheimer's disease. Numerous studies focused on the chemistry, bioactivities, toxicology, and clinical trials of HupA; however, the public genomic and transcriptomic resources are very limited for H. serrata research, especially for the selection of optimum reference genes. Based on the full‐length transcriptome datasets and previous studies, 10 traditional and three new candidate reference genes were selected in different tissue of H. serrata. Then, two optimal reference genes GAPDHB and HisH2A were confirmed by four analysis methods. In order to further verify the accuracy of the two reference genes, they were used to analyze the expression patterns of four HupA‐biosynthetic genes (lysine decarboxylas, RS‐norcoclaurine 6‐O‐methyltransferase, cytochrome P45072A1, and copper amine oxidase). The data suggested that the expression pattern of HupA‐biosynthetic genes was consistent with them in transcriptome sequencing in different tissue of H. serrata. This study identified that GAPDHB and HisH2A provides the reliable normalization for analyzing the HupA biosynthetic gene expression in different tissues of H. serrata on the transcriptional level. [ABSTRACT FROM AUTHOR]

Summary: Rice sheath blight caused by Rhizoctonia solani is the major disease of rice that seriously threatens food security worldwide. Efficient and eco‐friendly biological approaches are urgently needed since no resistant cultivars are available. In this study, fallow and paddy soils were initially subjected to microbiome analyses, and the results showed that Talaromyces spp. were significantly more abundant in the paddy soil, while Trichoderma spp. were more abundant in the fallow soil, suggesting that Talaromyces spp. could live and survive better in the paddy soil. Five Talaromyces isolates, namely, TF‐04, TF‐03, TF‐02, TF‐01 and TA‐02, were isolated from the paddy soil using sclerotia of R. solani as baits and were further evaluated for their activity against rice sheath blight. These isolates efficiently parasitized the hyphae and rotted the sclerotia even at higher water contents in the sterilized sand and the soil. Isolate TF‐04 significantly promoted rice growth, reduced the severity of rice sheath blight and increased the rice yield under outdoor conditions. Defence‐related genes were upregulated and enzyme activities were enhanced in rice treated with isolate TF‐04. Our research supplies a microbiome‐guided approach to screen biological control agents and provides Talaromyces isolates to biologically control rice sheath blight. [ABSTRACT FROM AUTHOR]

Summary: Fusarium graminearum is an important worldwide pathogen that causes Fusarium head blight in wheat, barley, maize and other grains. LncRNAs play important roles in many biological processes, but little is known about their functions and mechanisms in filamentous fungi. Here, we report that a natural antisense RNA, GzmetE‐AS, is transcribed from the opposite strand of GzmetE. GzmetE encodes a homoserine O‐acetyltransferase, which is important for sexual development and plant infection. The expression of GzmetE‐AS was increased significantly during the conidiation stage, while GzmetE was upregulated in the late stage of sexual reproduction. Overexpression of GzmetE‐AS inhibited the transcription of GzmetE. In contrast, the expression of GzmetE was significantly increased in GzmetE‐AS transcription termination strain GzmetE‐AS‐T. Furthermore, GzmetE‐AS‐T produced more perithecia and facilitated the ascospore discharge, resembling the phenotype of GzmetE overexpressing strains. However, overexpression of GzmetE‐AS in ∆dcl1/2 strain cannot inhibit the expression of GzmetE, and the GzmetE nat‐siRNA is also significantly reduced in ∆dcl1/2 mutant. Taken together, we have identified a novel antisense lncRNA GzmetE‐AS, which is involved in asexual and sexual reproduction by regulating its antisense gene GzmetE through RNAi pathway. Our findings reveal that the lncRNA plays critical roles in the development of F. graminearum. [ABSTRACT FROM AUTHOR]

In this paper, we propose a novel Multiview Stereo (MVS) method which can effectively estimate geometry in low‐textured regions. Conventional MVS algorithms predict geometry by performing dense correspondence estimation across multiple views under the constraint of epipolar geometry. As low‐textured regions contain less feature information for reliable matching, estimating geometry for low‐textured regions remains hard work for previous MVS methods. To address this issue, we propose an MVS method based on texture enhancement. By enhancing texture information for each input image via our multiscale bilateral decomposition and reconstruction algorithm, our method can estimate reliable geometry for low‐textured regions that are intractable for previous MVS methods. To densify the final output point cloud, we further propose a novel selective joint bilateral propagation filter, which can effectively propagate reliable geometry estimation to neighboring unpredicted regions. We validate the effectiveness of our method on the ETH3D benchmark. Quantitative and qualitative comparisons demonstrate that our method can significantly improve the quality of reconstruction in low‐textured regions. [ABSTRACT FROM AUTHOR]

Segmentation of transparent objects from sequences can be very useful in computer vision applications. However, without additional auxiliary information it can be hard work for traditional segmentation methods, as light in the transparent area captured by RGB cameras mostly derive from the background and the appearance of transparent objects changes with surroundings. In this article, we present a from‐coarse‐to‐fine transparent object segmentation method, which utilizes trajectory clustering to roughly distinguish the transparent from the background and refine the segmentation based on combination information of color and distortion. We further incorporate the transparency saliency with color and trajectory smoothness throughout the video to acquire a spatiotemporal segmentation based on graph‐cut. We conduct our method on various datasets. The results demonstrate that our method can successfully segment transparent objects from the background. [ABSTRACT FROM AUTHOR]

In this article, we propose the novel folding patch model which can replace the traditional patch model utilized in patch‐based multiview stereo (MVS) methods to significantly improve the reconstruction results. The patch model is applied as an approximation of the scene surface differential in the geometric estimation procedure. By minimizing the photometric discrepancy of the projection of the patch model on multiple source images, patch‐based MVS algorithms optimize the position and normal values for the 3D hypothesis of the target pixel. The optimization is based on the assumption that the patch model can fit the target scene surface perfectly. However, when it comes to complex scenes crowded with sharp edges, splintery surfaces, or round surfaces, the patch model is inherently not suitable since even from the microscopic perspective these surfaces are not entirely flat. We construct the folding patch model by folding the traditional patch model from the middle line. By adjusting the folding angle and direction, the folding patch model can fit complex surfaces more flexibly. We apply our folding patch model to the representative open‐source patch based multiview stereo (PMVS) and COLMAP, and validate the effectiveness on ETH3D benchmark and data sets captured in nature. The results demonstrate that utilizing the folding patch model can significantly improve the behavior of PMVS and COLMAP, especially on data sets mainly consist of complex surfaces from plants. [ABSTRACT FROM AUTHOR]

SsITL, a secretory protein of the necrotrophic phytopathogen Sclerotinia sclerotiorum, was previously reported to suppress host immunity at the early stages of infection. However, the molecular mechanism that SsITL uses to inhibit plant defence against S. sclerotiorum has not yet been elucidated. Here, we report that SsITL interacted with a chloroplast‐localized calcium‐sensing receptor, CAS, in chloroplasts. We found that CAS is a positive regulator of the salicylic acid signalling pathway in plant immunity to S. sclerotiorum and CAS‐mediated resistance against S. sclerotiorum depends on Ca2+ signalling. Furthermore, we showed that SsITL could interfere with the plant salicylic acid (SA) signalling pathway and SsITL‐expressing transgenic plants were more susceptible to S. sclerotiorum. However, truncated SsITLs (SsITL‐NT1 or SsITL‐CT1) that lost the ability to interact with CAS do not affect plant resistance to S. sclerotiorum. Taken together, our findings reveal that SsITL inhibits SA accumulation during the early stage of infection by interacting with CAS and then facilitating the infection by S. sclerotiorum. [ABSTRACT FROM AUTHOR]

In this paper, we propose a PatchMatch‐based Multi‐View Stereo (MVS) algorithm which can efficiently estimate geometry for the textureless area. Conventional PatchMatch‐based MVS algorithms estimate depth and normal hypotheses mainly by optimizing photometric consistency metrics between patch in the reference image and its projection on other images. The photometric consistency works well in textured regions but can not discriminate textureless regions, which makes geometry estimation for textureless regions hard work. To address this issue, we introduce the local consistency. Based on the assumption that neighboring pixels with similar colors likely belong to the same surface and share approximate depth‐normal values, local consistency guides the depth and normal estimation with geometry from neighboring pixels with similar colors. To fasten the convergence of pixelwise local consistency across the image, we further introduce a pyramid architecture similar to previous work which can also provide coarse estimation at upper levels. We validate the effectiveness of our method on the ETH3D benchmark and Tanks and Temples benchmark. Results show that our method outperforms the state‐of‐the‐art. [ABSTRACT FROM AUTHOR]

* Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. * Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. * Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). * Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense. [ABSTRACT FROM AUTHOR]

Coniothyrium minitans is an important sclerotial and hyphal parasite of the plant pathogen Sclerotinia sclerotiorum. Previously, a conidiation-deficient mutant, ZS-1N22225, was screened from a T-DNA insertional library of C. minitans. CmVps39, a homologue of Vam6p/Vps39p that plays a critical role in vacuolar morphogenesis in yeast, was disrupted by a T-DNA insertion in this mutant. CmVps39 is composed of 1071 amino acids with an amino-terminal citron homology domain and a central clathrin homology domain, as observed for other Vam6p/Vps39p family proteins. Abnormal fragmented vacuoles were observed in ΔCmVps39 under light microscopy and transmission electron microscopy, and ΔCmVps39 showed impairment in autophagy. ΔCmVps39 also exhibited significantly reduced hyphal development, poor conidiation and decreased sclerotial mycoparasitism. In addition, deletion of CmVps39 affected osmotic adaptation, pH homeostasis and cell wall integrity. Taken together, our results suggest that CmVps39 has an essential function in vacuolar morphology, autophagy, fungal development and mycoparasitism in C. minitans. [ABSTRACT FROM AUTHOR]

The actin cytoskeleton is involved in plant defense responses; however, the role of the actin-depolymerizing factor (ADF) family, which regulates actin cytoskeletal dynamics, in plant disease resistance, is largely unknown. Here, we characterized a wheat ( Triticum aestivum) ADF gene, TaADF7, with three copies located on chromosomes 1A, 1B, and 1D, respectively. All three copies encoded the same protein, although there were variations in 19 nucleotide positions in the open reading frame. Transcriptional expression of the three TaADF7 copies were all sharply elevated in response to avirulent Puccinia striiformis f. sp. tritici ( Pst) infection, with similar expression patterns. TaADF7 regulated the actin cytoskeletal dynamics by targeting the actin cytoskeleton to execute actin binding/severing activities. When the TaADF7 copies were all silenced by virus-induced gene silencing, the growth of Pst hypha increased and sporadic urediniospores were observed, as compared with control plants, upon inoculation with avirulent Pst. In addition, the accumulation of reactive oxygen species (ROS) and the hypersensitive response (HR) were greatly weakened, whereas cytochalasin B partially rescued the HR in TaADF7 knock-down plants. Together, these findings suggest that TaADF7 is likely to contribute to wheat resistance against Pst infection by modulating the actin cytoskeletal dynamics to influence ROS accumulation and the HR. [ABSTRACT FROM AUTHOR]

Transcription factors ( TFs) play crucial roles in the transcriptional regulation of plant development and defence responses. Increasing evidence has implicated ETHYLENE INSENSITIVE3 ( EIN3) in the plant defence response to pathogen infection and environmental stimuli. However, the role of EIN3 in wheat resistance to Puccinia striiformis f. sp. tritici ( Pst) is not clear. Here, TaEIL1 was isolated by rapid amplification of c DNA ends ( RACE) based on a sequence fragment from a wheat- Pst interaction c DNA library. The TaEIL1 protein contains a typical EIN3-binding domain, and transient expression analyses indicated that TaEIL1 is localized in the nucleus. Yeast one-hybrid assay revealed that TaEIL1 exhibits transcriptional activity, and its C-terminus is necessary for the activation of transcription. TaEIL1 transcripts were regulated by environmental stress stimuli and were decreased under salicylic acid ( SA) treatment. When wheat leaves were challenged with Pst, the transcript level of TaEIL1 in the compatible interaction was approximately three times higher than that in the incompatible interaction. Knocking down TaEIL1 through the Barley stripe mosaic virus ( BSMV) virus-induced gene silencing ( VIGS) system attenuated the growth of Pst, with shortened hyphae and reduced hyphal branches, haustorial mother cells and colony size. Moreover, enhanced necrosis was triggered by the Pst avirulent race CYR23, indicating that the hypersensitive response was strengthened in TaEIL1-silenced wheat plants. Thus, the up-regulation of defence-related genes and increased sucrose abundance might contribute to the enhanced disease resistance of wheat to the virulent race CYR31. Taken together, the results suggested that the suppression of TaEIL1 transcripts could enhance the resistance of wheat to stripe rust fungus. [ABSTRACT FROM AUTHOR]