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Promyelocytic leukemia (PML) protein forms the scaffold for PML nuclear bodies (PML NB) that reorganize into Lipid‐Associated PML Structures (LAPS) under fatty acid stress. We determined how the fatty acid oleate alters the interactome of PMLI or PMLII by expressing fusions with the ascorbate peroxidase APEX2 in U2OS cells. The resultant interactome included ESCRT and COPII transport protein nodes. Proximity ligation assay (PLA) revealed that COPII proteins SEC23B, SEC24A and USO1 preferentially associated with PML NBs. Nuclear localization of USO1, but not SEC23B and SEC24A, was reduced in PML knockout cells and restored by PMLII expression. Thus, proximity‐labelling methods identified COPII transport protein interactions with PML NBs that are disrupted by fatty acid stress. [ABSTRACT FROM AUTHOR]

Objective: Prenatal exome sequencing (pES) is now commonly used in clinical practice. It can be used to identifiy an additional diagnosis in around 30% of fetuses with structural defects and normal chromosomal microarray analysis (CMA). However, interpretation remains challenging due to the limited prenatal data for genetic disorders. Method: We conducted an ancillary study including fetuses with pathogenic/likely pathogenic variants identified by trio‐pES from the "AnDDI‐Prenatome" study. The prenatal phenotype of each patient was categorized as typical, uncommon, or unreported based on the comparison of the prenatal findings with documented findings in the literature and public phenotype‐genotype databases (ClinVar, HGMD, OMIM, and Decipher). Results: Prenatal phenotypes were typical for 38/56 fetuses (67.9%). For the others, genotype‐phenotype associations were challenging due to uncommon prenatal features (absence of recurrent hallmark, rare, or unreported). We report the first prenatal features associated with LINS1 and PGM1 variants. In addition, a double diagnosis was identified in three fetuses. Conclusion: Standardizing the description of prenatal features, implementing longitudinal prenatal follow‐up, and large‐scale collection of prenatal features are essential steps to improving pES data interpretation. Key points: What's already known about this topic? Prenatal exome sequencing detects an additional diagnosis in approximately 30% of fetuses with structural defects and normal chromosomal microarray analysis.Prenatal phenotyping may be challenging (limited examination of some organs, functional changes at birth, no intellectual or sensory information).Genetic disorders are highly heterogeneous. What does this study add? In 67.9% of cases, the association between the fetal phenotype and a relevant variant was straightforward. Conversely, it was challenging in 32.1% of cases due to unreported or uncommon prenatal findings.We reported prenatal findings associated with bi‐allelic LINS1 and PGM1 variants.Collaborative compilation of prenatal findings related to genetic disorders may lead to better understanding and identification of fetal conditions. [ABSTRACT FROM AUTHOR]

This article emphasizes the importance of considering the vertical dimension in socio-hydrosystem studies, particularly in relation to groundwater and subsurface flows. The authors argue that vertical connectivity has often been overlooked in previous research and call for better integration of the vertical dimension in socio-hydrological frameworks. They highlight the social and environmental implications of subsurface flows, including impacts on river ecosystems and water quality. The article also discusses the hyporheic zone, the interface between aquifers and rivers, and its importance in understanding and managing water resources. Overall, the article emphasizes the need for interdisciplinary research and a more inclusive and equitable approach to water resource management. [Extracted from the article]

Objective: Mitochondria are organelles that exhibit several bacterial features, such as a double‐stranded genome with hypomethylated CpG islands, formylated proteins, and cardiolipin‐containing membranes. In systemic lupus erythematosus (SLE), mitochondria and their inner components are released into the extracellular space, potentially eliciting a proinflammatory response from the immune system. While cardiolipin and mitochondrial DNA and RNA are confirmed targets of autoantibodies, other antigenic mitochondrial proteins in SLE remain to be identified. The present study was undertaken to characterize the protein repertoire recognized by antimitochondrial antibodies (AMAs) in patients with SLE. Methods: Using shotgun proteomic profiling, we identified 1,345 proteins, 431 of which were associated with the mitochondrial proteome. Immunoreactivities to several of these candidate proteins were assessed in serum samples from a local cohort (n = 30 healthy donors and 87 patients with SLE) using enzyme‐linked immunosorbent assay, and further analyzed for associations with demographic and disease characteristics. Results: We determined that IgG antibodies to the complement component C1q binding protein were significantly elevated in the patients with SLE (P = 0.049) and were also associated with lupus anticoagulant positivity (P = 0.049). Elevated levels of IgG antibodies against mitochondrial protein mitofusin 1 (MFN‐1) were promising predictors of SLE diagnosis in our cohort (adjusted odds ratio 2.99 [95% confidence interval 1.39–6.43], P = 0.0044). Moreover, increased levels of anti–MFN‐1 were associated with the presence of antiphospholipids (P = 0.011) and anti–double‐stranded DNA (P = 0.0005). Conclusion: In this study, we characterized the mitochondrial repertoire targeted by AMAs in the setting of SLE. Our results indicate that autoantibodies can recognize secreted and/or surface proteins of mitochondrial origin. Profiling of the AMA repertoire in large prospective cohorts may improve our knowledge of mitochondrial biomarkers and their usefulness for patient stratification. [ABSTRACT FROM AUTHOR]

The structure of M2(OH)2(bpdc) (bpdc = biphenyl dicarboxylate, C14H8O4) is distinct from that of the isoreticular compounds M2(OH)2(bdc) (bdc = benzene dicarboxylate, C8H4O4) (M = Mn, Fe), in the sense that no disorder of the bpdc molecules from one layer to the other needs to be considered. The global symmetry is lower in the bpdc compounds (P1) than in the bdc compounds (C2/m). Both Mn2(OH)2(bpdc) and Fe2(OH)2(bpdc) order magnetically at 36.8 and 46.5 K, respectively, and can be considered as uncompensated antiferromagnets, whereas Mn2(OH)2(bdc) (Néel temperature TN = 38.5 K) and Fe2(OH)2(bdc) (TN = 66 K) are compensated antiferromagnets. [ABSTRACT FROM AUTHOR]

RAB GTPases are central modulators of membrane trafficking. They are under the dynamic regulation of activating guanine exchange factors (GEFs) and inactivating GTPase‐activating proteins (GAPs). Once activated, RABs recruit a large spectrum of effectors to control trafficking functions of eukaryotic cells. Multiple proteomic studies, using pull‐down or yeast two‐hybrid approaches, have identified a number of RAB interactors. However, due to the in vitro nature of these approaches and inherent limitations of each technique, a comprehensive definition of RAB interactors is still lacking. By comparing quantitative affinity purifications of GFP:RAB21 with APEX2‐mediated proximity labeling of RAB4a, RAB5a, RAB7a, and RAB21, we find that APEX2 proximity labeling allows for the comprehensive identification of RAB regulators and interactors. Importantly, through biochemical and genetic approaches, we establish a novel link between RAB21 and the WASH and retromer complexes, with functional consequences on cargo sorting. Hence, APEX2‐mediated proximity labeling of RAB neighboring proteins represents a new and efficient tool to define RAB functions. Synopsis: Quantitative affinity purification combined with mass spectrometry (AP‐MS) and APEX2‐mediated proximity labeling of early endosomal RAB GTPases identify a novel interaction between RAB21 and the WASH/retromer complexes. Functional validation of the proteomic data defines a role for RAB21 in endosomal sorting of a subset of clathrin‐independent cargos. Unbiased proteomics establish a network of early endosomal RAB interacting and neighboring proteins.RAB21 interacts with the WASH and retromer complexes, and acts as a modulator of F‐actin generation at endosomes.RAB21 regulates sorting of a subset of clathrin‐independent cargos, such as MCT1, SLC3A2, Basigin and CD44. Quantitative proteomics and APEX2‐mediated proximity labeling of early endosomal RAB GTPases identify a novel interaction between RAB21 and the WASH/retromer complexes and defines a role for RAB21 in endosomal sorting of a subset of clathrin‐independent cargos. [ABSTRACT FROM AUTHOR]

By using acetyl-CoA as a substrate, acetyltransferases and histone deacetylases regulate protein acetylation by adding or removing an acetyl group on lysines. Nuclear-located Hdac1 is a regulator of intestinal homeostasis. We have previously shown that Hdac1 define specific intestinal epithelial cell basal and inflammatory-dependent gene expression patterns and control cell proliferation. We show here that Hdac1 depletion in cellulo leads to increased histone acetylation after metabolic stresses, and to metabolic disturbances resulting in impaired responses to oxidative stresses, AMPK kinase activation and mitochondrial biogenesis. Thus, nuclear Hdac1 may control intestinal epithelial cell metabolism by regulating the supply of acetyl groups. [ABSTRACT FROM AUTHOR]

This article aims to understand the relationships between rural-urban mobility and economic differentiation in Amazonian communities, through a multi-scaled methodological framework. We focus on Amazonian quilombola communities ( Pará State, Brazil), and we use a rural-urban circulation index to untangle the concept of mobility system. We show that individual mobility patterns are embedded in a process of multilocalisation, articulating rural and urban areas at a collective level. Economic diversification relies on complementarity of circulation within households. In this sense, the ability of some families to maintain resource rights in different places is one of the premises for economic differentiation. [ABSTRACT FROM AUTHOR]

The elucidation of the subcellular distribution of proteins under different conditions is a major challenge in cell biology. This challenge is further complicated by the multicompartmental and dynamic nature of protein localization. To address this issue, quantitative proteomics workflows have been developed to reliably identify the protein complement of whole organelles, as well as for protein assignment to subcellular location and relative protein quantification based on different cell culture conditions. Here, we review quantitative MS-based approaches that combine cellular fractionation with proteomic analysis. The application of these methods to the characterization of organellar composition and to the determination of the dynamic nature of protein complexes is improving our understanding of protein functions and dynamics. [ABSTRACT FROM AUTHOR]

A single crystal X-ray diffraction study was performed on the compounds [Ca2Al(OH)6] . [Cl∼0.5(CO3)∼0.25 .∼2.25H2O] belonging to the cementitious AFm family of general formulae [Ca2Al(OH)6] . [X. nH2O], where X is a monovalent anion, or half a divalent anion . The so-called chloro-carboaluminate compound crystallizes in the rhombohedral R3c space group with: a=5.7400(4)Å , c=46.7402(4)Å , V=1333.7(2)Å 3, Dx=2.054 g/cm-3, and Z=6. Refinement of 283 independent reflections led to a residual R factor of 0.020. Chloride and carbonate anions are statistically distributed into the same crystallographic site (6a Wyckoff site). The structure of the chloro-carboaluminate compound corresponds to the high temperature phase of Friedel's salt, the equivalent chloride AFm compound with composition [Ca2Al(OH)6] . [Cl .2H2O]. Three powdered samples of composition [Ca2Al(OH)6] . [Cl1-x(CO3)x/2 .∼2.25H2O], with x=0.25, 0.5, and 0.75, were synthesized and characterized in order to investigate the structure transition of Friedel's salt (from the rhombohedral HT-structure to the monoclinic LT-structure) versus the carbonate substitution level. Whereas a structure transition is observed around 35°C for the carbonate-free Friedel's salt, sample with x=0.25 shows a similar structure transition around -15°C. The two other samples with x=0.5 and 0.75, multiphase, exhibit a more complicated thermal behavior. [ABSTRACT FROM AUTHOR]

The Iratapuru Sustainable Development Reserve is mainly exploited by the community of São Francisco village. To improve their standard of living, this village organised its members around a production co-operative in 1992. Following considerable support from local and international institutions, this community became a symbol of sustainable development projects for 'traditional' communities of the Amazon. This was even more so after 2004, when an innovative partnership was established between the Brazilian cosmetics company NATURA and the village of São Francisco. Although the company claims that it associates its own economic interests with the priorities of the community, a number of questions arise nonetheless around this community-company deal, which our study tries to address. To that end, we first present the Jarí region and describe the Brazil nut production process in Iratapuru. Subsequently we give an overview of the development efforts in Iratapuru since the 1990s, focusing on the recent experience with NATURA. In the last part we analyse the NATURA-São Francisco relationship as a special case of a community-company deal in the Amazon. [ABSTRACT FROM AUTHOR]

Crystals of m-carboxyphenylammonium monohydrogenphos­phite, C7H8NO ·H2PO ( m-CPAMP), space group P2 / c, grown from aqueous solution undergo a reversible first-order single-crystal phase transition at Tc = 246 (2) K with a hysteresis of 3.6 K. The thermal behaviour of the sample was characterized by differential scanning calorimetry (DSC) experiments. Variations of the unit-cell parameters versus temperature between 100 and 320 K are reported. The transition from the higher-temperature phase (HTP) to the lower-temperature phase (LTP) is characterized by a unit-cell volume contraction of 1.77%. The average structure and unit-cell packing of m-CPAMP at lower temperature (100 K) are reported from accurate X-ray data sets and compared with those of the higher-temperature phase (293 K) in order to investigate the mechanism of the phase transition. The reciprocal lattice reconstruction showed a few very weak satellite reflections which will be discussed in a forthcoming paper. [ABSTRACT FROM AUTHOR]

The title compound, catena-poly­[[(heptanoato- O, O′)­lead(II)]-μ-heptanoato- O, O′: O: O′], [Pb(C7H13O2)2], is a metallic soap which can be used as a corrosion inhibitor since it forms a passive film at the Pb surface. Its structure is characterized by two-dimensional layers parallel to the bc plane. The layers are packed through van der Waals interactions along the a direction and form blocks parallel to (001). The 6 s2 lone pair of electrons on PbII is stereochemically active in this compound, which leads to a hemidirected octahedral geometry for the O-environment around the Pb atoms. [ABSTRACT FROM AUTHOR]

The title compound, calcium oxide-dialuminium trioxide-calcium dibromide-calcium dichloride hydrate (3/1/0.5/0.5/10), also formulated as Ca2Al(OH)6Br0.478Cl0.522·2H2O (dicalcium aluminium hydro­xide hemibromide hemichloride dihydrate), is a double-layered hydro­xide which belongs to the solid solution Ca2Al(OH)6Br xCl1− x·2H2O, where x can vary from 0 to 1. Chloride and bromide anions of the negatively charged interlayer [Br0.5Cl0.5·2H2O]− share statistically the same crystallographic site. Al3+ and Ca2+ cations are coordinated by six and seven O atoms, respectively. All water mol­ecules are bonded to Ca2+ cations and assume the seventh coordination position. Anions in the interlayer are surrounded by ten H atoms. Br− and Cl− are therefore connected to the main layer by ten hydrogen bonds, six of 2.74 (2) Å and four of 2.52 (5) Å, where the donors are hydroxyl groups and water mol­ecules, respectively. Like the chloride equivalent, the title compound is a 6 R polytype with trigonal space group R c and lattice parameters a = 5.7537 (4) Å and c = 48.108 (4) Å. [ABSTRACT FROM AUTHOR]

The structure of the title compound, poly­[zinc(II)-bis(μ-octanoato- O: O′)], [Zn(C8H15O2)2] n, consists of polymeric sheets parallel to (100) in which tetrahedrally coordinated Zn2+ cations are connected by carboxyl­ate bridges in a syn- anti arrangement. [ABSTRACT FROM AUTHOR]

In the title coordination polymer, [Ni(C8H4O4)(C4H10N2)] n, the Ni(II) atom is situated on a C2 axis and is six-coordinated by two N atoms from two piperazine (pip) mol­ecules, four O atoms from two bidentate and chelating terephthalate (tp) anions, forming a distorted octa­hedron. Both pip and tp mol­ecules, which are centrosymmetric, bridge adjacent Ni(II) atoms, forming a three-dimensional network. [ABSTRACT FROM AUTHOR]