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Biomaterials such as spider silk and mussel byssi are fabricated by the dynamic manipulation of intra‐ and intermolecular biopolymer interactions. Organisms modulate solution parameters, such as pH and ion co‐solute concentration, to effect these processes. These biofabrication schemes provide a conceptual framework to develop new dynamic and responsive abiotic soft material systems. Towards these ends, the chemical diversity of readily available ionic compounds offers a broad palette to manipulate the physicochemical properties of polyelectrolytes via ion‐specific interactions. In this study, we show for the first time that the ion‐specific interactions of biomimetic polyelectrolytes engenders a variety of phase separation behaviors, creating dynamic thermal‐ and ion‐responsive soft matter that exhibits a spectrum of physical properties, spanning viscous fluids to viscoelastic and viscoplastic solids. These ion‐dependent characteristics are further rendered general by the merger of lysine and phenylalanine into a single, amphiphilic vinyl monomer. The unprecedented breadth, precision, and dynamicity in the reported ion‐dependent phase behaviors thus introduce a broad array of opportunities for the future development of responsive soft matter; properties that are poised to drive developments in critical areas such as chemical sensing, soft robotics, and additive manufacturing. [ABSTRACT FROM AUTHOR]

Cable bacteria exhibit a unique metabolism involving long‐distance electron transport, significantly impacting elemental cycling in various sediments. These long filamentous bacteria are distributed circumglobally, suggesting an effective mode of dispersal. However, oxygen strongly inhibits their activity, posing a challenge to their dispersal through the water column. We investigated the effective dispersal of marine cable bacteria in a compartmentalised microcosm experiment. Cable bacteria were grown in natural 'source' sediment, and their metabolic activity was recorded in autoclaved 'destination' cores, which were only accessible through oxygenated seawater. Colonisation occurred over weeks, and destination cores contained only one cable bacterium strain. Filament 'snippets' (fragments with a median size of ~15 cells) accumulated in the microcosm water, with about 30% of snippets attached to sediment particles. Snippet release was also observed in situ in a salt marsh creek. This provides a model for the dispersal of cable bacteria through oxygenated water: snippets are formed by filament breakage in the sediment, released into the overlying water and transported with sediment particles that likely offer protection. These insights are informative for broader theories on microbial community assembly and prokaryotic biogeography in marine sediments. [ABSTRACT FROM AUTHOR]

Aim: The purpose of this study was to 1. investigate if glucose tolerance is affected after one acute bout of different types of exercise; 2. assess if potential differences between two exercise paradigms are related to changes in mitochondrial function; and 3. determine if endurance athletes differ from nonendurance‐trained controls in their metabolic responses to the exercise paradigms. Methods: Nine endurance athletes (END) and eight healthy nonendurance‐trained controls (CON) were studied. Oral glucose tolerance tests (OGTT) and mitochondrial function were assessed on three occasions: in the morning, 14 h after an overnight fast without prior exercise (RE), as well as after 3 h of prolonged continuous exercise at 65% of VO2max (PE) or 5 × 4 min at ~95% of VO2max (HIIT) on a cycle ergometer. Results: Glucose tolerance was markedly reduced in END after PE compared with RE. END also exhibited elevated fasting serum FFA and ketones levels, reduced insulin sensitivity and glucose oxidation, and increased fat oxidation during the OGTT. CON showed insignificant changes in glucose tolerance and the aforementioned measurements compared with RE. HIIT did not alter glucose tolerance in either group. Neither PE nor HIIT affected mitochondrial function in either group. END also exhibited increased activity of 3‐hydroxyacyl‐CoA dehydrogenase activity in muscle extracts vs. CON. Conclusion: Prolonged exercise reduces glucose tolerance and increases insulin resistance in endurance athletes the following day. These findings are associated with an increased lipid load, a high capacity to oxidize lipids, and increased fat oxidation. [ABSTRACT FROM AUTHOR]

Fibre type‐specific analyses are required for broader understanding of muscle physiology, but such analyses are difficult to conduct due to the extreme time requirements of dissecting and fibre typing individual fibres. Investigations are often confined to a small number of fibres from few participants with low representativeness of the entire fibre population and the participant population. To increase the feasibility of conducting large‐scale fibre type‐specific studies, a valid and rapid method for high‐throughput fibre typing of individually dissected fibres was developed and named THRIFTY (for high‐THRoughput Immunofluorescence Fibre TYping). Employing THRIFTY, 400 fibre segments were fixed onto microscope slides with a pre‐printed coordinated grid system, probed with antibodies against myosin heavy chain (MyHC)‐I and MyHC‐II and classified using a fluorescence microscope. The validity and speed of THRIFTY was compared to a previously validated protocol (dot blot) on a fibre‐to‐fibre basis. Fibre pool purity was evaluated using 'gold standard' SDS‐PAGE and silver staining. A modified THRIFTY‐protocol using fluorescence western blot equipment was also validated. THRIFTY displayed excellent agreement with the dot blot protocol, κ = 0.955 (95% CI: 0.928, 0.982), P < 0.001. Both the original and modified THRIFTY protocols generated type I and type II fibre pools of absolute purity. Using THRIFTY, 400 fibres were typed just under 11 h, which was approximately 3 times faster than dot blot. THRIFTY is a novel and valid method with high versatility for very rapid fibre typing of individual fibres. THRIFTY can therefore facilitate the generation of large fibre pools for more extensive mechanistic studies into skeletal muscle physiology. Key points: Skeletal muscle is composed of different fibre types, each with distinct physiological properties.To fully understand how skeletal muscle adapts to external cues such as exercise, nutrition and ageing, fibre type‐specific investigations are required.Such investigations are very difficult to conduct due to the extreme time requirements related to classifying individually isolated muscle fibres.To bypass this issue, we have developed a rapid and reliable method named THRIFTY which is cheap as well as versatile and which can easily be implemented in most laboratories.THRIFTY increases the feasibility of conducting larger fibre type‐specific studies and enables time‐sensitive assays where measurements need to be carried out in close connection with tissue sampling.By using THRIFTY, new insights into fibre type‐specific muscle physiology can be gained which may have broad implications in health and disease. [ABSTRACT FROM AUTHOR]

Coatings are used today for products, ranging from automotive production to electronics and everyday use items. Product design is taking on an increasingly important role, where effect pigments come to the fore, offering a coated surface extra optical characteristics. Individual effect pigments have strong anisotropic, azimuthaly‐dependent behaviour, typically suppressed by a coating application process, randomly orienting pigment particles resulting in isotropic appearance. One exception is a pigment that allows control of the azimuthal orientation of flakes using a magnetic field. We investigate visual texture effects due to such an orientation in a framework allowing efficient capturing, modelling and editing of its appearance. We captured spatially‐varying BRDFs of four coatings containing magnetic effect pigments. As per‐pixel non‐linear fitting cannot preserve coating sparkle effects, we suggest a novel method of anisotropy modelling based on images shifting in an angular domain. The model can be utilized for a fast transfer of desired anisotropy to any isotropic effect coating, while preserving important spatially‐varying visual features of the original coating. The anisotropic behaviour was fitted by a parametric model allowing for editing of coating appearance. This framework allows exploration of anisotropic effect coatings and their appearance transfer to standard effect coatings in a virtual environment. [ABSTRACT FROM AUTHOR]

The repair, remodeling, and regeneration of myofibers are dependent on satellite cells (SCs), although, the distribution of SCs in different fiber types of human muscle remains inconclusive. There is also a paucity of research comparing muscle fiber characteristics in a sex‐specific manner. Therefore, the aim of this study was to investigate fiber type‐specific SC content in men and women. Muscle biopsies from vastus lateralis were collected from 64 young (mean age 27 ± 5), moderately trained men (n = 34) and women (n = 30). SCs were identified by Pax7‐staining together with immunofluorescent analyses of fiber type composition, fiber size, and myonuclei content. In a mixed population, comparable number of SCs was associated to type I and type II fibers (0.07 ± 0.02 vs 0.07 ± 0.02 SCs per fiber, respectively). However, unlike men, women displayed a fiber type‐specific distribution, with SC content being lower in type II than type I fibers (P =.041). Sex‐based differences were found specifically for type II fibers, where women displayed lower SC content compared to men (P <.001). In addition, positive correlations (r‐values between 0.36‐0.56) were found between SC content and type I and type II fiber size in men (P =.03 and P <.01, respectively), whereas similar relationships could not be detected in women. Sex‐based differences were also noted for fiber type composition and fiber size, but not for myonuclei content. We hereby provide evidence for sex‐based differences present at the myocellular level, which may have important implications when studying exercise‐ and training‐induced myogenic responses in skeletal muscle. [ABSTRACT FROM AUTHOR]

Aims: The body responds to exercise training by profound adaptations throughout the cardiorespiratory and muscular systems, which may result in improvements in maximal oxygen consumption (VO2peak) and mitochondrial capacity. By convenience, mitochondrial respiration is often measured at supra‐physiological oxygen levels, an approach that ignores any potential regulatory role of mitochondrial affinity for oxygen (p50mito) at physiological oxygen levels. Methods: In this study, we examined the p50mito of mitochondria isolated from the Vastus lateralis and Triceps brachii in 12 healthy volunteers before and after a training intervention with seven sessions of sprint interval training using both leg cycling and arm cranking. The changes in p50mito were compared to changes in whole‐body VO2peak. Results: We here show that p50mito is similar in isolated mitochondria from the Vastus (40 ± 3.8 Pa) compared to Triceps (39 ± 3.3) but decreases (mitochondrial oxygen affinity increases) after seven sessions of sprint interval training (to 26 ± 2.2 Pa in Vastus and 22 ± 2.7 Pa in Triceps, both P <.01). The change in VO2peak modelled from changes in p50mito was correlated to actual measured changes in VO2peak (R2 =.41, P =.002). Conclusion: Together with mitochondrial respiratory capacity, p50mito is a critical factor when measuring mitochondrial function, it can decrease with sprint interval training and should be considered in the integrative analysis of the oxygen cascade from lung to mitochondria. [ABSTRACT FROM AUTHOR]

Cable bacteria are an emerging class of electroactive organisms that sustain unprecedented long‐range electron transport across centimeter‐scale distances. The local pathways of the electrical currents in these filamentous microorganisms remain unresolved. Here, the electrical circuitry in a single cable bacterium is visualized with nanoscopic resolution using conductive atomic force microscopy. Combined with perturbation experiments, it is demonstrated that electrical currents are conveyed through a parallel network of conductive fibers embedded in the cell envelope, which are electrically interconnected between adjacent cells. This structural organization provides a fail‐safe electrical network for long‐distance electron transport in these filamentous microorganisms. The observed electrical circuit architecture is unique in biology and can inspire future technological applications in bioelectronics. [ABSTRACT FROM AUTHOR]

Dark carbon fixation (DCF) by chemoautotrophic microorganisms can sustain food webs in the seafloor by local production of organic matter independent of photosynthesis. The process has received considerable attention in deep sea systems, such as hydrothermal vents, but the regulation, depth distribution, and global importance of coastal sedimentary DCF have not been systematically investigated. Here we surveyed eight coastal sediments by means of stable isotope probing (13C‐DIC) combined with bacterial biomarkers (phospholipid‐derived fatty acids) and compiled additional rates from literature into a global database. DCF rates in coastal sediments range from 0.07 to 36.30 mmol C m−2 day−1, and there is a linear relation between DCF and water depth. The CO2 fixation ratio (DCF/CO2 respired) also shows a trend with water depth, decreasing from 0.09 in nearshore environments to 0.04 in continental shelf sediments. Five types of depth distributions of chemoautotrophic activity are identified based on the mode of pore water transport (advective, bioturbated, and diffusive) and the dominant pathway of microbial sulfur oxidation. Extrapolated to the global coastal ocean, we estimate a DCF rate of 0.04 to 0.06 Pg C year−1, which is less than previous estimates based on indirect measurements (0.15 Pg C year−1), but remains substantially higher than the global DCF rate at deep sea hydrothermal vents (0.001–0.002 Pg C year−1). Key Points: Coastal dark carbon fixation rates are highest in diffusion‐driven intertidal areas and lowest in permeable continental shelf sedimentsPore water transport and microbial sulfur oxidation mechanisms determine five depth distribution models of dark carbon fixation in sedimentsThe renewal of labile organic matter by dark carbon fixation is important in coastal carbon budgets [ABSTRACT FROM AUTHOR]

Cable bacteria represent a newly discovered group of filamentous microorganisms, which are capable of spatially separating the oxidative and reductive half‐reactions of their sulfide‐oxidizing metabolisms over centimeter distances. We investigated three ways that cable bacteria might interact with the nitrogen (N) cycle: (1) by reducing nitrate through denitrification or dissimilatory nitrate reduction to ammonium (DNRA) within their cathodic cells; (2) by nitrifying ammonium within their anodic cells; and (3) by indirectly affecting denitrification and/or DNRA by changing the Fe2+ concentration in the surrounding sediment. We performed 15N labeling laboratory experiments to measure these three processes using cable bacteria containing sediments from the Yarra River, Australia, and from Vilhelmsborg Sø, Denmark. Our results revealed that in the targeted systems, cable bacteria themselves did not perform significant rates of denitrification, DNRA, or nitrification. However, cable bacteria exhibited an important indirect effect, whereby they increased the Fe2+ pool through iron sulfide dissolution. This elevated availability of Fe2+ significantly increased DNRA and in some cases decreased denitrification. Thus, cable bacteria presence may affect the relative importance of DNRA in sediments and thus the extent by which bioavailable nitrogen is lost from the system. [ABSTRACT FROM AUTHOR]

Summary Cable bacteria have been reported in sediments from marine and freshwater locations, but the environmental factors that regulate their growth in natural settings are not well understood. Most prominently, the physiological limit of cable bacteria in terms of oxygen availability remains poorly constrained. In this study, we investigated the presence, activity and diversity of cable bacteria in relation to a natural gradient in bottom water oxygenation in a depth transect of the Eastern Gotland Basin (Baltic Sea). Cable bacteria were identified by FISH at the oxic and transiently oxic sites, but not at the permanently anoxic site. Three species of the candidate genus Electrothrix, i.e. marina, aarhusiensis and communis were found coexisting within one site. The highest filament density (33 m cm−2) was associated with a 6.3 mm wide zone depleted in both oxygen and free sulphide, and the presence of an electric field resulting from the electrogenic sulphur oxidizing metabolism of cable bacteria. However, the measured filament densities and metabolic activities remained low overall, suggesting a limited impact of cable bacteria at the basin level. The observed bottom water oxygen levels (< 5 μM) are the lowest so far reported for cable bacteria, thus expanding their known environmental distribution. [ABSTRACT FROM AUTHOR]

Abstract: Cable bacteria are long, multicellular, filamentous bacteria that can conduct electrons over centimeter distances in marine and freshwater sediments. Recent studies indicate that cable bacteria are widely present in many coastal environments, where they exert a major influence on the biogeochemistry of the sediment. Their energy metabolism can be based on the aerobic oxidation of sulfide, and hence to better understand their natural occurrence and distribution, we examined the growth and activity of cable bacteria in relation to bottom water oxygenation. To this end, we conducted laboratory sediment incubations at four different O2 levels in the overlying water (10%, 20%, 40%, and 100% air saturation). The abundance of cable bacteria was determined by fluorescence in situ hybridization, while their activity was assessed via microsensor profiling and geochemical pore‐water analysis. Cable bacteria did not develop in the 10% air saturation O2 incubation but were present and active at all higher O2 levels. These data show that microbial long‐distance electron transport can occur under a wide range of bottom water O2 concentrations. However, the growth rate was notably slower at lower oxygen concentrations, suggesting a reduced metabolic activity of the population when the O2 supply becomes restricted. Finally, in response to lower O2 levels, cable bacteria filaments appear to partially emerge out of the sediment and extend into the overlying water, thus likely enhancing their oxygen supply. [ABSTRACT FROM AUTHOR]

Accelerated mass loss from the Greenland ice sheet leads to glacier retreat and an increasing input of glacial meltwater to the fjords and coastal waters around Greenland. These high latitude ecosystems are highly productive and sustain important fisheries, yet it remains uncertain how they will respond to future changes in the Arctic cryosphere. Here we show that marine-terminating glaciers play a crucial role in sustaining high productivity of the fjord ecosystems. Hydrographic and biogeochemical data from two fjord systems adjacent to the Greenland ice sheet, suggest that marine ecosystem productivity is very differently regulated in fjords influenced by either land-terminating or marine-terminating glaciers. Rising subsurface meltwater plumes originating from marine-terminating glaciers entrain large volumes of ambient deep water to the surface. The resulting upwelling of nutrient-rich deep water sustains a high phytoplankton productivity throughout summer in the fjord with marine-terminating glaciers. In contrast, the fjord with only land-terminating glaciers lack this upwelling mechanism, and is characterized by lower productivity. Data on commercial halibut landings support that coastal regions influenced by large marine-terminating glaciers have substantially higher marine productivity. These results suggest that a switch from marine-terminating to land-terminating glaciers can substantially alter the productivity in the coastal zone around Greenland with potentially large ecological and socio-economic implications. [ABSTRACT FROM AUTHOR]

Seasonal hypoxia refers to the oxygen depletion that occurs in summer in the bottom water of stratified systems, and is increasingly observed in coastal areas worldwide. The process induces a seasonal cycle on the biogeochemistry of the underlying sediments, which remains poorly quantified. Here, we investigated the sedimentary oxygen consumption within Lake Grevelingen (The Netherlands), a saline coastal reservoir that is impacted by yearly recurrent bottom water hypoxia. Monthly sampling campaigns were conducted throughout 2012 at three sites along a depth gradient. Macrofauna sampling and sediment profile imaging demonstrated how summer hypoxia strongly impacts the benthic communities below 15 m of water depth. Benthic fluxes of oxygen, dissolved inorganic carbon, total alkalinity, and ammonium were determined by closed core incubations, while oxygen depth profiles were recorded by microsensor profiling of sediment cores. Our results reveal a pronounced seasonality in the sedimentary oxygen consumption. Low uptake rates in summer were caused by oxygen limitation, and resulted in the build-up of an 'oxygen debt' through the accumulation of reduced iron sulfides. Highest oxygen uptake rates were recorded in fall, linked to the reoxidation of the pool of iron sulfides in the top layer. However, uptake rates remained unexpectedly high during winter and early spring, likely associated with the oxidation of iron sulfides down to centimeters depth due to the electrogenic sulfur oxidation by cable bacteria. Overall, our results suggest that the sedimentary oxygen dynamic in seasonally hypoxic coastal systems is characterized by a strongly amplified 'oxygen debt' dynamics induced by cable bacteria. [ABSTRACT FROM AUTHOR]

One of the most accurate yet still practical representation of material appearance is the Bidirectional Texture Function (BTF). The BTF can be viewed as an extension of Bidirectional Reflectance Distribution Function (BRDF) for additional spatial information that includes local visual effects such as shadowing, interreflection, subsurface-scattering, etc. However, the shift from BRDF to BTF represents not only a huge leap in respect to the realism of material reproduction, but also related high memory and computational costs stemming from the storage and processing of massive BTF data. In this work, we argue that each opaque material, regardless of its surface structure, can be safely substituted by a BRDF without the introduction of a significant perceptual error when viewed from an appropriate distance. Therefore, we ran a set of psychophysical studies over 25 materials to determine so-called critical viewing distances, i.e. the minimal distances at which the material spatial structure (texture) cannot be visually discerned. Our analysis determined such typical distances typical for several material categories often used in interior design applications. Furthermore, we propose a combination of computational features that can predict such distances without the need for a psychophysical study. We show that our work can significantly reduce rendering costs in applications that process complex virtual scenes. [ABSTRACT FROM AUTHOR]

This study investigated the relationship between aerobic efficiency during cycling exercise and the increase in physical performance with acute hyperoxic exposure (FiO2 ~ 31%) (HOX) and also tested the hypothesis that fat oxidation could be increased by acute hyperoxia. Fourteen males and four females were recruited for two sessions, where they exercised for 2 × 10 min at 100 W to determine efficiency. HOX and normoxia (NOX) were administered randomly on both occasions to account for differences in nitrogen exchange. Thereafter, a progressive ramp test was performed to determine VO2max and maximal power output (Wmax). After 30 min rest, workload was set to 80% of maximal power output (Wmax) for a time to exhaustion test (TTE). At 100W gross efficiency was reduced from 19.4% during NOX to 18.9% during HOX (P ≤ 0.0001). HOX increased fat oxidation at 100 W by 52% from 3.41 kcal min-1 to 5.17 kcal min-1 (P ≤ 0.0001) with a corresponding reduction in carbohydrate oxidation. Wmax increased by 2.4% from 388.8 (±82.1) during NOX to 397.8 (±83.5) during HOX (P ≤ 0.0001). SaO2 was higher in HOX both at the end of the maximal exercise test and TTE. Subjects with a high level of efficiency in NOX had a larger improvement in Wmax with HOX, in agreement with the hypothesis that an optimum level of efficiency exists that maximizes power production. No association between mitochondrial excess capacity and endurance performance was found; increases in oxygen supply seemed to increase maximal aerobic power production and maintain/increase endurance capacity at the same relative workload. [ABSTRACT FROM AUTHOR]

BRDFs are commonly used for material appearance representation in applications ranging from gaming and the movie industry, to product design and specification. Most applications rely on isotropic BRDFs due to their better availability as a result of their easier acquisition process. On the other hand, anisotropic BRDF due to their structure-dependent anisotropic highlights, are more challenging to measure and process. This paper thus leverages the measurement process of anisotropic BRDF by representing such BRDF by the collection of isotropic BRDFs. Our method relies on an anisotropic BRDF database decomposition into training isotropic slices forming a linear basis, where appropriate sparse samples are identified using numerical optimization. When an unknown anisotropic BRDF is measured, these samples are repeatably captured in a small set of azimuthal directions. All collected samples are then used for an entire measured BRDF reconstruction from a linear isotropic basis. Typically, below 100 samples are sufficient for the capturing of main visual features of complex anisotropic materials, and we provide a minimal directional samples to be regularly measured at each sample rotation. We conclude, that even simple setups relying on five bidirectional samples (maximum of five stationary sensors/lights) in combination with eight rotations (rotation stage for specimen) can yield a promising reconstruction of anisotropic behavior. Next, we outline extension of the proposed approach to adaptive sampling of anisotropic BRDF to gain even better performance. Finally, we show that our method allows using standard geometries, including industrial multi-angle reflectometers, for the fast measurement of anisotropic BRDFs. [ABSTRACT FROM AUTHOR]

Surface reflectance of real-world materials is now widely represented by the bidirectional reflectance distribution function (BRDF) and also by spatially varying representations such as SVBRDF and the bidirectional texture function (BTF). The raw surface reflectance measurements are typically compressed or fitted by analytical models, that always introduce a certain loss of accuracy. For its evaluation we need a distance function between a reference surface reflectance and its approximate version. Although some of the past techniques tried to reflect the perceptual sensitivity of human vision, they have neither optimized illumination and viewing conditions nor surface shape. In this paper, we suggest a new image-based methodology for comparing different anisotropic BRDFs. We use optimization techniques to generate a novel surface which has extensive coverage of incoming and outgoing light directions, while preserving its features and frequencies that are important for material appearance judgments. A single rendered image of such a surface along with simultaneously optimized lighting and viewing directions leads to the computation of a meaningful BRDF difference, by means of standard image difference predictors. A psychophysical experiments revealed that our surface provides richer information on material properties than the standard surfaces often used in computer graphics, e.g., sphere or blob. [ABSTRACT FROM AUTHOR]

Intense exercise training is a powerful stimulus that activates mitochondrial biogenesis pathways and thus increases mitochondrial density and oxidative capacity. Moderate levels of reactive oxygen species (ROS) during exercise are considered vital in the adaptive response, but high ROS production is a serious threat to cellular homeostasis. Although biochemical markers of the transition from adaptive to maladaptive ROS stress are lacking, it is likely mediated by redox sensitive enzymes involved in oxidative metabolism. One potential enzyme mediating such redox sensitivity is the citric acid cycle enzyme aconitase. In this study, we examined biopsy specimens of vastus lateralis and triceps brachii in healthy volunteers, together with primary human myotubes. An intense exercise regimen inactivated aconitase by 55-72%, resulting in inhibition of mitochondrial respiration by 50-65%. In the vastus, the mitochondrial dysfunction was compensated for by a 15-72% increase in mitochondrial proteins, whereas H2O2 emission was unchanged. In parallel with the inactivation of aconitase, the intermediary metabolite citrate accumulated and played an integral part in cellular protection against oxidative stress. In contrast, the triceps failed to increase mitochondrial density, and citrate did not accumulate. Instead, mitochondrial H2O2 emission was decreased to 40% of the pretraining levels, together with a 6-fold increase in protein abundance of catalase. In this study, a novel mitochondrial stress response was highlighted where accumulation of citrate acted to preserve the redox status of the cell during periods of intense exercise.--Larsen, F. J., Schiffer, T. A., Ørtenblad, N., Zinner, C., Morales-Alamo, D., Willis, S. J., Calbet, J. A., Holmberg, H.-C., Boushel, R. High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation. [ABSTRACT FROM AUTHOR]

BRDFs are commonly used to represent given materials' appearance in computer graphics and related fields. Although, in the recent past, BRDFs have been extensively measured, compressed, and fitted by a variety of analytical models, most research has been primarily focused on simplified isotropic BRDFs. In this paper, we present a unique database of 150 BRDFs representing a wide range of materials; the majority exhibiting anisotropic behavior. Since time-consuming BRDF measurement represents a major obstacle in the digital material appearance reproduction pipeline, we tested several approaches estimating a very limited set of samples capable of high quality appearance reconstruction. Initially, we aligned all measured BRDFs according to the location of the anisotropic highlights. Then we propose an adaptive sampling method based on analysis of the measured BRDFs. For each BRDF, a unique sampling pattern was computed, given a predefined count of samples. Further, template-based methods are introduced based on reusing of the precomputed sampling patterns. This approach enables a more efficient measurement of unknown BRDFs while preserving the visual fidelity for the majority of tested materials. Our method exhibits better performance and stability than competing sparse sampling approaches; especially for higher numbers of samples. [ABSTRACT FROM AUTHOR]

The maximum power principle dictates that open biological systems tend to self-organize to a level of efficiency that allows maximal power production. Applying this principle to cellular energetics and whole-body physiology would suggest that for every metabolic challenge, an optimal efficiency exists that maximizes power production. On exposure to hypoxia, it would be favorable if metabolic efficiency would rapidly adjust so as to better preserve work performance. We tested this idea in humans by measuring metabolic efficiency and exercise tolerance under nor-moxic (FiO2=20.9%) and hypoxic (FiO2=16%) conditions, where FiO2 is fraction of inhaled oxygen. The results were compared with respirometric analyses of skeletal muscle mitochondria from the same individuals. We found that among healthy trained subjects (n = 14) with a wide range of metabolic efficiency (ME), those with a high ME during normoxic exercise were able to better maintain exercise capacity (Wmax) in hypoxia. On hypoxic exposure, these subjects acutely decreased their efficiency from 19.2 to 17.4%, thereby likely shifting it closer to a degree of efficiency where maximal power production is achieved. In addition, mitochondria from these subjects had a lower intrinsic respiration compared to subjects that showed a large drop in Wmax in hypoxia An acute shift in efficiency was also demonstrated in isolated mitochondria exposed to physiological levels of hypoxia as P/O ratio increased from 0.9 to 1.3 with hypoxic exposure. These findings suggest the existence of a physiological adaptive response by which metabolic efficiency is dynamically optimized to maximize power production. [ABSTRACT FROM AUTHOR]

The basal metabolic rate (BMR) is referred to as the minimal rate of metabolism required to support basic body functions. It is well known that individual BMR varies greatly, even when correcting for body weight, fat content, and thyroid hormone levels, but the mechanistic determinants of this phenomenon remain unknown. Here, we show in humans that mass-related BMR correlates strongly to the mitochondrial oxygen affinity (p50mito; R²=0.66, P=0.0004) measured in isolated skeletal muscle mitochondria. A similar relationship was found for oxygen affinity and efficiency during constant-load submaximal exercise (R²=0.46, P=0.007). In contrast, BMR did not correlate to overall mitochondrial density or to proton leak. Mechanistically, part of the p50mito seems to be controlled by the excess of cytochrome c oxidase (COX) protein and activity relative to other mitochondrial proteins. This is illustrated by the 5-fold increase in p50mito after partial cyanide inhibition of COX at doses that do not affect maximal mitochondrial electron flux through the ETS. These data suggest that the interindividual variation in BMR in humans is primarily explained by differences in mitochondrial oxygen affinity. The implications of these findings are discussed in terms of a trade-off between aerobic efficiency and power. [ABSTRACT FROM AUTHOR]

Uptake of anthropogenic CO2 is acidifying the oceans. Over the next 2000 years, this will modify the dissolution and preservation of sedimentary carbonate. By coupling new formulas for the positions of the calcite saturation horizon, Zsat, the compensation depth, Zcc, and the snowline, Zsnow, to a biogeochemical model of the oceanic carbonate system, we evaluate how these horizons will change with ongoing ocean acidification. Our model is an extended Havardton-Bear-type box model, which includes novel kinetic descriptions for carbonate dissolution above, between, and below these critical depths. In the preindustrial ocean, Zsat and Zcc, are at 3939 and 4750 m, respectively. When forced with the IS92a CO2 emission scenario, the model forecasts (1) that zsat will rise rapidly ("runaway" conditions) so that all deep water becomes undersaturated, (2) that Zcc, will also rise and over 1000 years will pass before it will be stabilized by the dissolution of previously deposited CaCO3, and (3) that Zsnow will respond slowly to acidification, rising by ∼1150 m during a 2000 year timeframe. A further simplified model that equates the compensation and saturation depths produces quantitatively different results. Finally, additional feedbacks due to acidification on calcification and increased atmospheric CO2 on organic matter productivity strongly affect the positions of the compensation horizons and their dynamics. [ABSTRACT FROM AUTHOR]

The Bidirectional Texture Function (BTF) is becoming widely used for accurate representation of real-world material appearance. In this paper a novel BTF compression model is proposed. The model resamples input BTF data into a parametrization, allowing decomposition of individual view and illumination dependent texels into a set of multi-dimensional conditional probability density functions. These functions are compressed in turn using a novel multi-level vector quantization algorithm. The result of this algorithm is a set of index and scale code-books for individual dimensions. BTF reconstruction from the model is then based on fast chained indexing into the nested stored code-books. In the proposed model, luminance and chromaticity are treated separately to achieve further compression. The proposed model achieves low distortion and compression ratios 1:233–1:2040, depending on BTF sample variability. These results compare well with several other BTF compression methods with predefined compression ratios, usually smaller than 1:200. We carried out a psychophysical experiment comparing our method with LPCA method. BTF synthesis from the model was implemented on a standard GPU, yielded interactive framerates. The proposed method allows the fast importance sampling required by eye-path tracing algorithms in image synthesis. [ABSTRACT FROM AUTHOR]

The physical mechanism that drives bioirrigation is strongly dependent on the permeability of the sediment. We advance two mechanisms, each described by a corresponding microenvironment model. In muds, burrow water cannot penetrate the sediment, so bioirrigation is intrinsically driven by diffusional transfer across the burrow wall. This "diffusive" mode of bioirrigation is accurately described by the classical tube irrigation model. In sands, ventilation flows can penetrate the surrounding sediment via dead end burrows. To quantify this "advective" mode of bioirrigation, we propose a novel two-dimensional pocket injection model. This model's principal features are that (1) organisms indent the sediment-water interface with burrow structures, (2) the specific structure of the burrow can be neglected except for the location of a feeding pocket, and (3) burrow water is injected from this feeding pocket into the surrounding sediment. We tested the adequacy of the pocket injection model in a detailed case study of the lugworm Arenicola marina, comparing model simulations and experimental data from core incubations. Simulation of two different sets of inert tracer experiments shows good agreement between model and data, indicating that our model captures the relevant aspects of lugworm bioirrigation in permeable sediments. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented discussing a case of serious iatrogenic disease in an elderly caused by accidental ingestion of press-through medication package.

To investigate diel calcium carbonate (CaCO3) dynamics in permeable coral reef sands, we measured pore-water profiles and fluxes of oxygen (O2), nutrients, pH, calcium (Ca2+), and alkalinity (TA) across the sediment-water interface in sands of different permeability at Heron Reef, Australia. Background flushing rates were high, most likely as a result of infaunal burrow irrigation, but flux chamber stirring enhanced pore-water exchange. Light and pore-water advection fueled high rates of benthic primary production and calcification in sunlit surface sediments. In the light, benthic photosynthesis and calcification induced surface minima in Ca2+ and TA and peaks in pH and O2. Oxygen penetration depth in coarse sands decreased from ∼ 1.2cm during the day to ∼ 0.6cm at night. Total oxygen uptake (TOU) in dark chambers was three to fourteen times greater than diffusive uptake and showed a direct effect of pore-water advection. Greater sediment oxygen consumption rates were observed in higher permeability sands. In the dark, TA release was not stimulated by increasing TOU because of a damping effect of pore-water advection on metabolic CaCO3 dissolution efficiency. On a daily basis, CaCO3 undergoes net dissolution in Heron Reef sands. However, pore-water advection can reverse the CaCO3 budget and promote CaCO3 preservation under the most energetic conditions.