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Purpose: To compare phase‐resolved functional lung (PREFUL) regional ventilation derived from a free breathing 3D UTE radial MRI acquisition to hyperpolarized 129Xe‐MRI (Xe‐MRI), conventional 2D multi‐slice PREFUL MRI, and pulmonary function tests in pediatric cystic fibrosis (CF) lung disease. Methods: Free‐breathing 3D UTE and 2D multi‐slice 1H MRI as well as Xe‐MRI were acquired in 12 stable pediatric CF patients. Using PREFUL, regional ventilation (RVent) maps were calculated from the free‐breathing data. Ventilation defect percentage (VDP) was determined from 3D and 2D RVent maps (2D VDPRVent and 3D VDPRVent, respectively) and Xe‐MRI ventilation (VDPXe). VDP was calculated for the whole lung and for eight regions based on left/right, anterior/posterior, and superior/inferior divisions of the lung. Global and regional VDP was compared between the three methods using Bland–Altman analysis, linear mixed model‐based correlation, and one‐way analysis of variance and multiple comparisons tests. Results: Global 3D VDPRVent, VDPXe, and 2D VDPRVent were all strongly correlated (all R2 > 0.62, p < 0.0001) and showed minimal, non‐significant bias (all <2%, p > 0.05). Three dimensional and 2D VDPRVent significantly correlated to VDPXe in most of the separate lung regions (R2 = 0.18–0.74, p < 0.04), but showed lower inter‐agreement. The superior/anterior lung regions showed the least agreement between all three methods (all p > 0.12). Conclusion: Absolute VDP assessed by 3D UTE PREFUL MRI showed good global agreement with Xe‐MRI and 2D multi‐slice PREFUL MRI in pediatric CF lung disease. Therefore, 3D UTE PREFUL MRI offers a sensitive and potentially more accessible alternative to Xe‐MRI for regional volumetric evaluation of ventilation. [ABSTRACT FROM AUTHOR]

Among the developmental milestones related to language and communication in early childhood, one that has been the subject of considerable research is response to name (RTN). Delayed or absent RTN in early childhood is a diagnostic marker for autism spectrum disorder and a target behavior in many early intervention curricula. This article describes two related studies. Study 1 evaluated the efficacy and efficiency of a behavioral screening‐to‐intervention model for RTN proposed by recent research. Overall, trials to mastery were reduced relative to previous research. Study 2 evaluated the efficacy of using behavioral skills training to teach caregivers to implement a RTN intervention with their child after that intervention was successful in a clinical setting. Generalized improvements in RTN with caregivers sometimes occurred but did not maintain without programmed reinforcement. Subsequent behavioral skills training was associated with increases in both child RTN and caregiver intervention fidelity. [ABSTRACT FROM AUTHOR]

We present an assessment of the water mass dynamics in a reanalysis of the Mediterranean Sea with a focus on the Algero‐Provencal basin. We use a θ‐S‐based algorithm to compute the fractions of the main western Mediterranean water masses: Atlantic and modified Atlantic Waters (AW and mAW), Western and Eastern Intermediate Waters (WIW and EIW), Tyrrhenian and Western Mediterranean Deep Water (TDW and WMDW). The reanalysis retains the known mean characteristics of the water masses, their seasonal to interannual variabilities, and main circulation patterns when compared with the literature. The imprint of winter mixing is particularly obvious with coherent variations of water mass volumes, mainly the yearly creation of WIW from mAW on northernmost shelves and of WMDW from all surface and intermediate layers during years of deep water formation (DWF). The results also highlight some unrealistic events of variability of the WMDW volume that are likely due to the data assimilation process. Recomputation of water mass volumes and transports without these altered years allowed to highlight the possible disruption of large‐scale barotropic cyclonic circulation in the East Algerian basin in response to major DWF events over the Gulf of Lion and the induced surface consequence on Algerian Eddies' trajectories. Plain Language Summary: Defined as the large‐scale oceanic circulation driven by surface heat and freshwater flows, oceanic thermohaline circulation plays a major role in the Earth's climate, as it distributes excess heat and carbon dioxide due to human activities into the deeper layers of the ocean over the long term. The Mediterranean Sea is unique in that it has its own thermohaline circulation due to its semi‐enclosed configuration, a climate‐driven evaporation (∼1 m/y) balanced by an influx of Atlantic Water, and significant winter heat loss leading to the formation of heavy water masses at the surface and their sinking toward the intermediate and bottom layers. This thermohaline circulation has a time scale of less than 100 years, more than 10 times smaller than the global circulation, and has been shown to respond rapidly to climate variability in the Northern Hemisphere. We have used a 20‐year ocean reanalysis—a simulation assimilating observations—to characterize and quantify water mass circulation in the Algero‐Provencal basin, from seasonal to interannual scales. Our study reveals that the main weakness of reanalysis lies in deep‐water dynamics, whereas it has a marked impact on surface and intermediate circulations. Understanding the Mediterranean's future requires a better representation of its deep‐water dynamics. Key Points: We study the dynamics of seven water masses in a 20‐year reanalysis using a linear mixing algorithm based on θ‐S based fractionsThe method allows detecting spurious events of deep water formation, destruction and surface impact, likely due to the assimilation processResults show that the Provencal deep water formation could slow down the deep Algerian gyre, inducing an effect on the surface eddies' path [ABSTRACT FROM AUTHOR]

Valproic acid (VA) is a widely used drug for the treatment of diseases affecting the central nervous system. Due to its epigenetic modulatory potential, it has been studied for possible therapeutic application in anticancer therapies. However, the VA exhibits different side effects in its application. Thus, synthetic coordination complexes with valproate can generate promising candidates for new active drugs with reduced toxicity. In this sense, we investigated the genotoxic and mutagenic potential of the sodium valproate and of the mixed ternary mononuclear Mg complex based on VA with 1,10‐phenanthroline (Phen) ligand ‐ [Mg (Valp)2Phen], in Saccharomyces cerevisiae and V79 cells. The MTT and clonal survival assays in V79 cells indicated that the Mg complex has higher cytotoxicity than sodium valproate. A similar cytotoxicity profile is observed in yeast. This fact is possibly due to the intercalation capacity of [Mg(Valp)2Phen], inducing DNA strand breaks, as observed in the comet assay and micronucleus test. In this sense, members of the NER, HR, NHEJ and TLS repair pathways are required for the repair of DNA lesions induced by [Mg(Valp)2Phen]. Interestingly, BER proteins apparently increase the cytotoxic potential of the drug. Furthermore, the [Mg(Valp)2Phen] showed higher cytotoxicity in V79 cells and yeast when compared to sodium valproate indicating applicability as a cytotoxic agent. [ABSTRACT FROM AUTHOR]

Cities are concentrators of complex, multi‐sectoral interactions. As keystones in the interconnected human‐Earth system, cities have an outsized impact on the Earth system. We describe a multi‐lens framework for organizing our understanding of the complexity of urban systems and scientific research on urban systems, which may be useful for natural system scientists exploring the ways their work can be made more actionable. We then describe four critical dimensions along which improvements are needed to advance the urban research that addresses urgent climate challenges: (a) solutions‐oriented research, (b) equity‐centered assessments which rely on fine‐scale human and ecological data, (c) co‐production of knowledge, and (d) better integration of human and natural systems occurring through theory, observation, and modeling. Plain Language Summary: Cities can be seen as concentrators of complex, multi‐sectoral interactions: ripples of influence across different systems travel faster, through more systems, and have greater consequences within cities than in other contexts. We describe a multi‐lens framework for organizing our understanding of the complexity of urban systems and of scientific research on urban systems. We then describe four important improvements to urban research so we can better address urgent climate challenges in cities and globally. Key Points: Cities are concentrators of complex, multi‐sectoral interactionsIn order to address climate risks, people need transformative solutions tailored to their problems and at decision‐relevant scalesA richer understanding of human and natural interactions in urban environments can support our transition to a more climate secure future [ABSTRACT FROM AUTHOR]

Process‐induced deformation (PID) arises in thermoset parts due to internal residual stress developed from their anisotropic properties, resulting in distortions. While passive numerical manufacturing control exists, active manufacturing control is crucial for enhancing the manufacturing process. The work focuses on diagnosing the polymerization reaction, known as the curing process, to consider the influence of uncertainties in thermal loading conditions on the behavior of cure kinetics. This is achieved using a Particle Filter approach, wherein a posterior distribution of cure evolution is recursively approximated based on observed measurements from characterization tests. The algorithm is designed to simultaneously perform the diagnosis and prognosis of the Degree of Cure and PID. This approach adopts the augmented cure formulation to address various scenarios with uncertainties in thermal loading conditions. It offers the advantage of providing comparable PID predictions with minimal computational costs. C‐shaped thermoset parts made of epoxy/carbon fibers with varying thicknesses are cured using the Manufacturing Recommended Curing Cycle, and the predictions with the developed algorithm are validated against experimental measures. Upon validation, the converged prognosis capability of the Particle Filter model is employed to assess the impact of thermal loading uncertainty on cure profiles, which, in turn, affects the final PIDs outcome. Highlights: A Bayesian sampling approach enables the estimation of cure kinetics parameters.The estimated stochastic parameters forecast the process‐induced deformations.The augmented Degree of Cure accounts for uncertainties linked to thermal loadings.Analysis on AS4/8552 C‐shaped parts shows the cure kinetics impact.The framework reduces the computational costs required for active control. [ABSTRACT FROM AUTHOR]

Mitochondria are vital for most cells' functions. Viruses hijack mitochondria machinery for misappropriation of energy supply or to bypass defense mechanisms. Many of these mitochondrial dysfunctions persist after recovery from treated or untreated viral infections, particularly when mitochondrial DNA is permanently damaged. Quantitative defects and structural rearrangements of mitochondrial DNA accumulate in post‐mitotic tissues as recently reported long after SARS‐CoV‐2 or HIV infection, or following antiviral therapy. These observations are consistent with the "hit‐and‐run" concept proposed decades ago to explain viro‐induced cell transformation and it could apply to delayed post‐viral onsets of symptoms and advocate for complementary supportive care. Thus, according to this concept, following exposure to viruses or antiviral agents, mitochondrial damage could evolve into an autonomous clinical condition. It also establishes a pathogenic link between communicable and non‐communicable chronic diseases. [ABSTRACT FROM AUTHOR]

The Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6) is the primary effort of CMIP6 (Coupled Model Intercomparison Project–Phase 6) focusing on ice sheets, designed to provide an ensemble of process‐based projections of the ice‐sheet contribution to sea‐level rise over the twenty‐first century. However, the behavior of the Antarctic Ice Sheet beyond 2100 remains largely unknown: several instability mechanisms can develop on longer time scales, potentially destabilizing large parts of Antarctica. Projections of Antarctic Ice Sheet evolution until 2300 are presented here, using an ensemble of 16 ice‐flow models and forcing from global climate models. Under high‐emission scenarios, the Antarctic sea‐level contribution is limited to less than 30 cm sea‐level equivalent (SLE) by 2100, but increases rapidly thereafter to reach up to 4.4 m SLE by 2300. Simulations including ice‐shelf collapse lead to an additional 1.1 m SLE on average by 2300, and can reach 6.9 m SLE. Widespread retreat is observed on that timescale in most West Antarctic basins, leading to a collapse of large sectors of West Antarctica by 2300 in 30%–40% of the ensemble. While the onset date of retreat varies among ice models, the rate of upstream propagation is highly consistent once retreat begins. Calculations of sea‐level contribution including water density corrections lead to an additional ∼10% sea level and up to 50% for contributions accounting for bedrock uplift in response to ice loading. Overall, these results highlight large sea‐level contributions from Antarctica and suggest that the choice of ice sheet model remains the leading source of uncertainty in multi‐century projections. Plain Language Summary: Numerical models simulating the evolution of the Antarctic Ice Sheet have mostly focused on the twenty‐first century. How the ice sheet will evolve after 2100 remains highly uncertain, as several instability mechanisms could develop and destabilize vast regions of Antarctica. We investigate here the behavior of the Antarctic Ice Sheet until 2300 using an ensemble of 16 different ice flow models. The results show that the Antarctic contribution to sea‐level rise remains limited until 2100 but increases rapidly afterward. The ice retreats in most basins of the West Antarctic Ice Sheet, and some numerical experiments suggest a near‐complete collapse of this region by 2300. The time when these glaciers start retreating varies depending on the choice of ice flow model, but the speed at which they retreat is consistent among the models once the retreat begins. On a multi‐century timescale, the choice of ice sheet model remains a leading source of uncertainties. Key Points: Antarctic Ice Sheet mass loss and associated uncertainty increase sharply after 2100Ice streams feeding the Ross and Ronne ice shelves experience considerable and consistent grounding line retreat for all ice sheet modelsOn multi‐centennial timescales, uncertainty in mass loss remains dominated by the choice of ice flow model, followed by the climate forcing [ABSTRACT FROM AUTHOR]

Introduction: New tools have been developed to distinguish the COVID‐19 diagnosis from other viral infections presenting similar symptomatology and mitigate the lack of sensitivity of molecular testing. We previously identified a specific "sandglass" aspect on the white blood cells (WBC) scattergram of COVID‐19 patients, as a highly reliable COVID‐19 screening test (sensitivity: 85.9%, specificity: 83.5% and positive predictive value: 94.3%). We then decided to validate our previous data in a multicentric study. Methods: This retrospective study involved 817 patients with flu‐like illness, among 20 centers, using the same CBC instrument (XN analyzer, SYSMEX, Japan). After training, one specialist per center independently evaluated, under the same conditions, the presence of the "sandglass" aspect of the WDF scattergram, likely representing plasmacytoid lymphocytes. Results: Overall, this approach showed sensitivity: 59.0%, specificity: 72.9% and positive predictive value: 77.7%. Sensitivity improved with subgroup analysis, including in patients with lymphopenia (65.2%), patients presenting symptoms for more than 5 days (72.3%) and in patients with ARDS (70.1%). COVID‐19 patients with larger plasmacytoid lymphocyte cluster (>15 cells) more often have severe outcomes (70% vs. 15% in the control group). Conclusion: Our findings confirm that the WBC scattergram analysis could be added to a diagnostic algorithm for screening and quickly categorizing symptomatic patients as either COVID‐19 probable or improbable, especially during COVID‐19 resurgence and overlapping with future influenza epidemics. The observed large size of the plasmacytoid lymphocytes cluster appears to be a hallmark of COVID‐19 patients and was indicative of a severe outcome. Furthers studies are ongoing to evaluate the value of the new hematological parameters in combination with WDF analysis. [ABSTRACT FROM AUTHOR]

Trilaciclib is a first‐in‐class, intravenous cyclin‐dependent kinase 4 and 6 inhibitor approved for reducing the incidence of chemotherapy‐induced myelosuppression in adult patients with extensive‐stage small cell lung cancer receiving a platinum/etoposide‐containing or topotecan‐containing regimen. No dose adjustment is recommended for participants with mild hepatic impairment (HI) based on previous population pharmacokinetic (PK) analysis. This open‐label, parallel‐group study examined the impact of moderate and severe HI on the PK of trilaciclib. The study employed a reduced study design. Participants with moderate (Child–Pugh B, n = 8) and severe (Child–Pugh C, n = 5) HI and matched healthy controls (n = 11) received a single intravenous dose of trilaciclib 100 mg/m2. The unbound fraction of trilaciclib was comparable between the HI groups and the matched healthy control group. The unbound trilaciclib extent of exposure (i.e., area under the concentration‐time curve) in participants with moderate and severe HI was ∼40% and ∼60% higher, respectively, compared with healthy matched controls based on Child–Pugh classification. Ad hoc analysis using National Cancer Institute classification showed similar results. The US Food and Drug Administration‐approved trilaciclib dose of 240 mg/m2 should be reduced by ∼30%, to 170 mg/m2, for patients with moderate or severe HI. [ABSTRACT FROM AUTHOR]

N‐methyl‐d‐aspartate receptors (NMDARs) at hippocampal excitatory synapses undergo a late postnatal shift in subunit composition, from an initial prevalence of GluN2B subunit incorporation to a later predominance of GluN2A. This GluN2B to GluN2A shift alters NMDAR calcium conductance dynamics and intracellular molecular signaling that are individually regulated by distinct GluN2 signaling domains and temporally align with developmental alterations in dendritic and synaptic plasticity. However, the impacts of individual GluN2B to GluN2A signaling domains on neuronal development remain unknown. Ionotropic and intracellular signaling domains of GluN2 subunits were separated by creating chimeric GluN2 subunits that were expressed in two transgenic mouse lines. Western blot and immunoprecipitation revealed that roughly one third of native synaptic NMDARs were replaced by transformed NMDARs without altering total synaptic NMDAR content. Schaffer collateral synaptic strength was transiently increased in acutely prepared hippocampal slices at just over 3 weeks of age in animals overexpressing the GluN2B carboxy terminus. Long‐term potentiation (LTP) induction following lower frequency stimulation was regulated by GluN2 ionotropic signaling domains in an age‐dependent manner and LTP maintenance was enhanced by overexpression of the GluN2B CTD in mature animals. After higher frequency stimulation, the induction and maintenance of LTP were increased in young adult animals overexpressing the GluN2B ionotropic signaling domains but reduced in juveniles just over 3 weeks of age. Confocal imaging of green fluorescent protein (GFP)‐ labeled CA1 pyramidal neurons revealed no alterations in dendritic morphology or spine density in mice expressing chimeric GluN2 subunits. These results illustrate how individual GluN2 subunit signaling domains do or do not control physiological and morphological development of hippocampal excitatory neurons and better clarify the neurobiological factors that govern hippocampal maturation. SIGNIFICANCE STATEMENT: A developmental reduction in the magnitude of hippocampal long‐term synaptic potentiation (LTP) and a concomitant improvement in spatial maze performance coincide with greater incorporation of GluN2A subunits into synaptic NMDARs. Corroborating our prior discovery that overexpression of GluN2A‐type ionotropic signaling domains enables context‐based navigation in immature mice, GluN2A‐type ionotropic signaling domain overexpression reduces LTP induction threshold and magnitude in immature mice. Also, we previously found that GluN2B carboxy terminal domain (CTD) overexpression enhances long‐term spatial memory in mature mice and now report that the GluN2B CTD is associated with greater amplitude of LTP after induction in mature mice. Thus, the late postnatal maturation of context encoding likely relies on a shift toward GluN2A‐type ionotropic signaling and a reduction in the threshold to induce LTP while memory consolidation and LTP maintenance are regulated by GluN2B subunit CTD signaling. [ABSTRACT FROM AUTHOR]

Objective: The copepod Parvocalanus crassirostris has recently increased its use as live food in marine fish hatcheries. Methods: To investigate the bacterial community of P. crassirostris nauplii and explore the potential use of nauplii as a vector for probiotics, adult copepods were reared with or without a probiotic consortium (Bacillus spp. and Lactobacillus spp.). Nauplii from both treatments were either enriched with probiotics or not, resulting in four treatments: control rearing and nonenriched, control rearing and probiotic enriched, probiotic rearing and nonenriched, and probiotic rearing probiotic enriched. A next‐generation sequencing analysis of the 16S ribosomal RNA gene was performed. Result: The bacterial community of nauplii without probiotics was dominated by the phyla Proteobacteria (mean ± standard deviation = 51.5 ± 8.0%) and Bacteroidetes (27.6 ± 6.7%); from these phyla, the most abundant orders were Rhodobacterales (21.8 ± 2.0%), Flavobacteriales (13.5 ± 9.4%), Chitinophagales (9.7 ± 1.8%), Alteromonadales (9.5 ± 4.0%), and Burkholderiales (3.8 ± 0.8%). Opportunistic pathogenic bacterial genera, such as Vibrio spp. and Pseudomonas spp., were detected at very low relative abundances (<1.2%), indicating that P. crassirostris nauplii could be considered a safe microbiological live feed. Furthermore, P. crassirostris nauplii were a good vector for probiotics delivery, as the adhesion of probiotics on the copepod exoskeleton was successful. Bacillus spp. abundance was 5–6× greater than the abundance of Lactobacillus spp. in P. crassirostris nauplii. The highest mean values of probiotics relative abundance (Bacillus spp. 41 ± 6.0%, Lactobacillus spp. 6.0 ± 3.0%) were observed in nauplii that were reared with probiotics and also enriched with probiotics. Conclusion: Parvocalanus crassirostris nauplii stages I–II appear to be good vectors to provide probiotics to first‐feeding fish larvae. Impact statementMarine fish aquaculture relies on quality live feed for optimal protein production. Assessing copepod nauplii's microbiological safety as fish larvae prey and their role as probiotics vectors is an important contribution to marine fish aquaculture. [ABSTRACT FROM AUTHOR]

The size and growth patterns of nestling birds are key determinants of their survival up to fledging and long‐term fitness. However, because traits such as feathers, skeleton and body mass can follow different developmental trajectories, our understanding of the impact of adverse weather on development requires insights into trait‐specific sensitive developmental windows. We analysed data from nestling Alpine swifts in Switzerland measured throughout growth up to the age of 50 days (i.e. fledging between 50 and 70 days), for wing length and body mass (2693 nestlings in 25 years) and sternum length (2447 nestlings in 22 years). We show that the sensitive developmental windows for wing and sternum length corresponded to the periods of trait‐specific peak growth, which span almost the whole developmental period for wings and the first half for the sternum. Adverse weather conditions during these periods slowed down growth and reduced size. Although nestling body mass at 50 days showed the greatest inter‐individual variation, this was explained by weather in the two days before measurement rather than during peak growth. Interestingly, the relationship between temperature and body mass was not linear, and the initial sharp increase in body mass associated with the increase in temperature was followed by a moderate drop on hot days, likely linked to heat stress. Nestlings experiencing adverse weather conditions during wing growth had lower survival rates up to fledging and fledged at later ages, presumably to compensate for slower wing growth. Overall, our results suggest that measures of feather growth and, to some extent, skeletal growth best capture the consequences of adverse weather conditions throughout the whole development of offspring, while body mass better reflects the short, instantaneous effects of weather conditions on their body reserves (i.e. energy depletion vs. storage in unfavourable vs. favourable conditions). [ABSTRACT FROM AUTHOR]

Beyond ecological and health impacts, invasive alien plant species can generate indirect and direct costs, notably through reduced agricultural yields, restoration, and management of the invaded environment. Acacia dealbata and Ailanthus altissima are invasive plant species that cause particularly significant damage to the railway network in the Mediterranean area. The allelopathic properties of Mediterranean plant species could be used as nature‐based solutions to slow down the spread of such invasive plant species along railway borders. In this context, a mesocosm experiment was set‐up: (i) to test the potential allelopathic effects of Cistus ladanifer, Cistus albidus, and Cotinus coggygria leaf aqueous extracts on seed germination and seedling growth of A. dealbata and A. altissima; (ii) to evaluate whether these effects depend on the extract dose; and finally, (iii) to estimate whether these effects are modified by soil amendment. Leaf aqueous extracts of the three native plant species showed negative effects on both seed germination and seedling growth of the two invasive species. Our results show that the presence of allelochemicals induces a delay in seed germination (e.g., A. dealbata germination lasted up to 269% longer in the presence of high‐dose leaf aqueous extracts of C. coggygria), which can lead to a decrease in individual recruitment. They also highlight a decrease in seedling growth (e.g., high‐dose C. coggygria leaf aqueous extracts induced a 26% decrease in A. dealbata radicle growth), which can alter the competitiveness of invasive species for resource access. Our results also highlight that compost addition limits the inhibitory effect of native Mediterranean plants on the germination of invasive alien plants, suggesting that soil organic matter content can counteract allelopathic effects on invasive alien plants. Thus, our findings revealed that the allelopathic potential of certain Mediterranean plant species could be a useful tool to manage invasive plant species. [ABSTRACT FROM AUTHOR]

Creativity can be assessed using various methods, including divergent thinking performance, self‐ratings, and teacher ratings. However, these measures may not always align, as they may not consistently identify creative potential in the same manner. The present study aimed to identify latent subgroups of students based on their observed originality, creative self‐efficacy, teacher‐rated originality, academic achievement in reading and mathematics, and demographic background characteristics. Data were collected from 243 elementary school students. We applied the normal mixture technique to classify participants into latent subgroups. Five latent subgroups of students were identified: Overconfident Low Performers, Creative High Achievers, Under‐Confident Below‐Average Achievers, Mathematically Oriented Students, and Calibrated Above‐Average Achievers. Female students tended to fall disproportionately into the subgroup of Creative High Achievers. Students receiving free/reduced lunch had a lower probability of being Creative High Achievers. Special education students had a higher probability of falling into the subgroup Overconfident Low Performers. Teacher ratings of students' originality were more in line with student academic performance rather than with their performance‐based originality scores. Students' self‐ratings of creativity bifurcated across subgroups, with Creative High Achievers and Overconfident Low Performers reporting the highest self‐ratings of originality, despite displaying very different levels of performance on the divergent thinking assessment. [ABSTRACT FROM AUTHOR]

Straits and narrows are the place of intensified turbulent mixing. Deep understanding of the turbulent dynamics at these locations is of crucial importance as it conditions the properties of the water masses flowing in the open ocean. A new extensive field experiment, PROTEVS GIB20, with high frequency measurements has been conducted in the Strait of Gibraltar. It allows us to infer dissipation rates of the turbulent kinetic energy, ϵ, from two consistent methods. The range of ϵ is depicted for the different processes which developed in the vicinity of Camarinal Sill, the main topographic feature of the Strait of Gibraltar. It evidences that the bottom boundary layer, hydraulic jumps and large overturns are the main loci of intensified turbulence reaching 10−3W.kg−1. The variability of the turbulence is mainly controlled by semi‐diurnal, diurnal and fortnightly tidal oscillations. Spatially, the western flank of Camarinal Sill is evidenced as the hotspot for turbulent mixing. We confirm a weak variation of the spatially averaged vertically integrated turbulent dissipation rate. This result needs to be qualified in view of the differentiated impact of the various processes on adjacent water masses. The dynamics of the spring tide directly mix Atlantic and Mediterranean waters, resulting in a large spreading of the T‐S diagram. Plain Language Summary: A recent field experiment, PROTEVS GIB20, was performed in the Strait of Gibraltar in October 2020. This experiment was partly designed to observe the turbulent dynamics of the flow in this region. The Strait of Gibraltar presents an important topographic sill, Camarinal Sill, where the current reaches its maximum value enhancing the turbulent processes. Quantifying the turbulence intensity and the associated mixing that develops there is of crucial importance to understand the impact of small‐scale process on larger/regional scales which is now recognized but remains incompletely understood. Such measurements are necessary to assess the performances of the parametrization use in climate model which to dot represent such small‐scale dynamics. Direct measurements of dissipation rate of turbulent kinetic energy are rare near Camarinal Sill, due to the rough environment and strong maritime traffic. From different methods, we infer time series and spatial averages of the turbulence dissipation rate. We describe the variability of the turbulence, the physical processes involved and the impact on water mass mixing. Key Points: Dissipation rates of TKE are inferred near the Camarinal Sill with maximum values of 10−3 W/kg in the hydraulic jumpThe spatio‐temporal distribution of dissipation rates near Camarinal Sill shows maxima on the western flank in tidal outflowsMixing rates are similar during spring and neap tides except for the Atlantic waters more impacted by spring tide hydraulic jumps [ABSTRACT FROM AUTHOR]

Objective: The objective of this study was to evaluate the implementation of NGS within the French mitochondrial network, MitoDiag, from targeted gene panels to whole exome sequencing (WES) or whole genome sequencing (WGS) focusing on mitochondrial nuclear‐encoded genes. Methods: Over 2000 patients suspected of Primary Mitochondrial Diseases (PMD) were sequenced by either targeted gene panels, WES or WGS within MitoDiag. We described the clinical, biochemical, and molecular data of 397 genetically confirmed patients, comprising 294 children and 103 adults, carrying pathogenic or likely pathogenic variants in nuclear‐encoded genes. Results: The cohort exhibited a large genetic heterogeneity, with the identification of 172 distinct genes and 253 novel variants. Among children, a notable prevalence of pathogenic variants in genes associated with oxidative phosphorylation (OXPHOS) functions and mitochondrial translation was observed. In adults, pathogenic variants were primarily identified in genes linked to mtDNA maintenance. Additionally, a substantial proportion of patients (54% (42/78) and 48% (13/27) in children and adults, respectively), undergoing WES or WGS testing displayed PMD mimics, representing pathologies that clinically resemble mitochondrial diseases. Interpretation: We reported the largest French cohort of patients suspected of PMD with pathogenic variants in nuclear genes. We have emphasized the clinical complexity of PMD and the challenges associated with recognizing and distinguishing them from other pathologies, particularly neuromuscular disorders. We confirmed that WES/WGS, instead of panel approach, was more valuable to identify the genetic basis in patients with "possible" PMD and we provided a genetic testing flowchart to guide physicians in their diagnostic strategy. [ABSTRACT FROM AUTHOR]

Aim: The aim of this study is to contribute to an understanding of the role deployment of advanced practice nurses (APNs) in French healthcare settings. Introduction: The introduction of APNs was formalised in France by the decrees issued on 18 July 2018, which described the areas, activities and training of APNs. Background: A qualitative study on the role implementation of APNs was conducted between July 2021 and May 2022 following a call for projects launched by the Île‐de‐France Regional Health Agency to evaluate the deployment of APNs in the area. Methods: Data were collected through field observations and semi‐structured interviews in order to explore both the APNs deployment processes in nine healthcare structures and the roles played by APN networks and associations with regard to the deployment of APN activities in their working environments. Results: The projects proved to be evolutionary, and their development was marked by various forms of APN isolation and multiple obstacles that were specific to their professional practice settings. Some APNs relied on a variety of forms of mutual assistance and advocacy deployed throughout APN networks and associations. Discussion: The deployment of APNs' role was impacted by diverse configurations of professional power relations and the nature of the obstacles that were structural for APNs in primary care. Their experience of isolation derived from the novelty of their role, the challenge they posed to the cohesion of the nursing profession and a lack of supportive policies for their deployment. Their participation in APN networks and associations enabled them to access advocacy and manage the uncertainties and unknowns related to the deployment of their activities. Conclusion: The results suggest that the formalisation of schemes for mutual assistance among APNs and advocacy should be integrated into the guidelines for the implementation of their role. Implications for nursing policy: APN policy should strengthen a bottom‐up approach, relying in particular on the development of different forms of collaboration and communication between APN networks and associations on the one hand and the public authorities on the other. [ABSTRACT FROM AUTHOR]

Seasonal evolution of both surface signature and subsurface structure of a Mediterranean mesoscale anticyclones is assessed using the Coastal and Regional Ocean Community high‐resolution numerical model with realistic background stratification and fluxes. In good agreement with remote‐sensing and in‐situ observations, our numerical simulations capture the seasonal cycle of the anomalies induced by the anticyclone, both in the sea surface temperature (SST) and in the mixed layer depth (MLD). The eddy signature on the SST shifts from warm‐core in winter to cold‐core in summer, while the MLD deepens significantly in the core of the anticyclone in late winter. Our sensitivity analysis shows that the eddy SST anomaly can be accurately reproduced only if the vertical resolution is high enough (∼4 m in near surface) and if the atmospheric forcing contains high‐frequency. In summer with this configuration, the vertical mixing parameterized by the k − ϵ closure scheme is three times higher inside the eddy than outside the eddy, and leads to an anticyclonic cold core SST anomaly. This differential mixing is explained by near‐inertial waves, triggered by the high‐frequency atmospheric forcing. Near‐inertial waves propagate more energy inside the eddy because of the lower effective Coriolis parameter in the anticyclone core. On the other hand, eddy MLD anomaly appears more sensitive to horizontal resolution, and requires SST retroaction on air‐sea fluxes. These results detail the need of high frequency forcing, high vertical and horizontal resolutions to accurately reproduce the evolution of a mesoscale eddy. Plain Language Summary: Mesoscale eddies are turbulent structures present in every regions of the world ocean, and accounting for a significant part of its kinetic energy budget. These structures can be tracked in time and recently revealed a seasonal cycle from in situ data. An anticyclone (clockwise rotating eddy in the northern hemisphere) is observed in the Mediterranean to be predominantly warm at the surface and to deepen the mixed layer in winter, but shifts to a cold‐core summer signature. This seasonal signal is not yet understood and studied in ocean models. In this study we assess the realism of an anticyclone seasonal evolution in high resolution numerical simulations. Eddy surface temperature seasonal shift is retrieved and is linked to an increased mixing at the eddy core spontaneously appearing at high vertical resolution (vertical grid size smaller than 4 m) in the presence of high frequency atmospheric forcing. This increased mixed is due to the preferred propagation of near‐inertial waves in the anticyclone due to its negative relative vorticity. Eddy‐induced mixed layer depth anomalies also appear to be triggered by sea surface temperature retroaction on air‐sea fluxes. These results suggest that present‐day operational ocean forecast models are too coarse to accurately retrieve mesoscale evolution. Key Points: Enhanced mixing in anticyclones explain inverse eddy sea surface temperature (SST) signatureVertical resolution is crucial to model eddy core mixing triggered by near‐inertial wavesMixed layer anomaly is mainly driven by SST retroaction on air‐sea fluxes [ABSTRACT FROM AUTHOR]

In most animals, body mass varies with ecological conditions and is expected to reflect how much energy can be allocated to reproduction and survival. Because the sexes often differ in their resource acquisition and allocation strategies, variations in adult body mass and their consequences on fitness can differ between the sexes.Assessing the relative contributions of environmental and genetic effects (i.e. heritability)—and whether these effects and their fitness consequences are sex‐specific—is essential to gain insights into the evolution of sexual dimorphism and sexual conflicts.We used 20+ years of data to study the sources of variation in adult body mass and associated fitness consequences in a bird with biparental care, the Alpine swift (Tachymarptis melba). Swifts appear monomorphic to human observers, though subtle dimorphisms are present.We first investigated the effects of weather conditions on adult body mass using a sliding window analysis approach. We report a positive effect of temperature and a negative effect of rainfall on adult body mass, as expected for an aerial insectivorous bird. We then quantified the additive genetic variance and heritability of body mass in both sexes and assessed the importance of genetic constraints on mass evolution by estimating the cross‐sex genetic correlation. Heritability was different from zero in both sexes at ~0.30. The positive cross‐sex genetic correlation and comparable additive genetic variance between the sexes suggest the possibility for evolutionary constraints when it comes to body mass. Finally, we assessed the sex‐specific selection on adjusted body mass using multiple fitness components. We report directional positive and negative selection trending towards stabilizing and diversifying selection on females and males respectively in relation to the weighted proportion of surviving fledglings.Overall, these results suggest that while body mass may be able to respond to environmental conditions and evolve, genetic constraints would result in similar changes in both sexes or an overall absence of response to selection. It remains unclear whether the weak (1%) dimorphism in Alpine swift body mass we report is simply a result of the similar fitness peaks between the sexes or of genetic constraints. [ABSTRACT FROM AUTHOR]

Fitness in micro‐organisms can be proxied by growth parameters on different media and/or temperatures. This is achieved by measuring optical density at 600 nm using a spectrophotometer, which measures the effect of absorbance and side scattering due to turbidity of cells suspensions. However, when growth kinetics must be monitored in many 96‐well plates at the same time, buying several 96‐channel spectrophotometers is often beyond budgets. The MiniRead device presented here is a simple and inexpensive do‐it‐yourself 96‐well temperature‐controlled turbidimeter designed to measure the interception of white light via absorption or side scattering through liquid culture medium. Turbidity is automatically recorded in each well at regular time intervals for up to several days or weeks. Output tabulated text files are recorded into a micro‐SD memory card to be easily transferred to a computer. We propose also an R package which allows (1) to compute the nonlinear calibration curves required to convert raw readings into cell concentration values, and (2) to analyze growth kinetics output files to automatically estimate proxies of growth parameters such as lag time, maximum growth rate, or cell concentration at the plateau. Take‐away: We present a simple and inexpensive do‐it‐yourself turbidimeter for cell suspensions. It records automatically micro‐organisms growth kinetics in 96‐well plates without computer. Temperature is controlled in plate bottom and lid independently to avoid mist on the lids. An associated R package allows automatic kinetics analysis to estimate growth parameters. [ABSTRACT FROM AUTHOR]

The combination of physical equations with deep learning is becoming a promising methodology for bioprocess digitalization. In this paper, we investigate for the first time the combination of long short‐term memory (LSTM) networks with first principles equations in a hybrid workflow to describe human embryonic kidney 293 (HEK293) culture dynamics. Experimental data of 27 extracellular state variables in 20 fed‐batch HEK293 cultures were collected in a parallel high throughput 250 mL cultivation system in an industrial process development setting. The adaptive moment estimation method with stochastic regularization and cross‐validation were employed for deep learning. A total of 784 hybrid models with varying deep neural network architectures, depths, layers sizes and node activation functions were compared. In most scenarios, hybrid LSTM models outperformed classical hybrid Feedforward Neural Network (FFNN) models in terms of training and testing error. Hybrid LSTM models revealed to be less sensitive to data resampling than FFNN hybrid models. As disadvantages, Hybrid LSTM models are in general more complex (higher number of parameters) and have a higher computation cost than FFNN hybrid models. The hybrid model with the highest prediction accuracy consisted in a LSTM network with seven internal states connected in series with dynamic material balance equations. This hybrid model correctly predicted the dynamics of the 27 state variables (R2 = 0.93 in the test data set), including biomass, key substrates, amino acids and metabolic by‐products for around 10 cultivation days. [ABSTRACT FROM AUTHOR]

This pilot study involved the development and initial testing of an applied Multicultural Orientation Performance Task (MCO‐PT) and coding system. The MCO‐PT was comprised of seven brief video simulations of cultural exchanges. Seventy‐four graduate students recorded responses and then completed measures of desirable responding and multicultural competence. Research assistants coded the videos on three dimensions: cultural humility, cultural opportunities and cultural comfort (ICCs 0.61–0.97). Exploratory factor analysis yielded three factors that corresponded with the MCO constructs (alphas 0.77–0.97). Cultural opportunities were positively correlated with multicultural competence, and none of the subscales were associated with social desirability. [ABSTRACT FROM AUTHOR]

Understanding the colonization process of species living in a dynamic fragmented habitat is essential to assess their persistence. In the metapopulation theory, the colonization of a species can be quantified using the turnover of occupancy in habitat patches. However, this approach is often limited by the feasible size of surveyed areas. Because many species are capable of long‐distance dispersal, such areas often constitute open systems undergoing colonization of propagules coming from outside, the 'background deposition'. We focus on disentangling background deposition from local colonization among surveyed patches when analyzing turnover. We consider two spatial scales: 1) focal areas where all patches are monitored over time; 2) a larger extent, encompassing the focal areas, over which the distribution of the target species is quantified with a coarse spatial grain. Our key idea is to use the regional connectivity of focal areas within the larger scale as a covariate when analyzing colonization events within focal areas. A positive effect of regional connectivity on the colonization probability of patches may indicate background deposition. We applied this approach to the epiphytic bryophyte Dicranum viride in a managed temperate deciduous forest, considering phorophyte trees as patches, forest stands as focal areas and the whole forest as the larger scale. We combined a fine‐grained turnover survey of occupied trees within three forest stands (~ 3 ha) with a coarse‐grained snapshot of D. viride distribution over the forest (~ 15 000 ha). Regional connectivity came out as the most significant factor, with a strong positive effect on colonization probability within stands. However, it was attributed to sources in the immediate vicinity of focal stands, suggesting a short‐ranged colonization process occurring across stands' borders rather than long‐distance background deposition. Our results thus call for maintaining a stepping‐stone of habitat across the forest through time to improve D. viride persistence. [ABSTRACT FROM AUTHOR]

Objective: Red Snapper Lutjanus campechanus is an iconic species in the southeast U.S. Atlantic Ocean, sought by both commercial and recreational fleets. Five stock assessments over the past quarter century have shown Red Snapper to be experiencing overfishing. Highly restricted landings since 2010 have been insufficient to end overfishing because fishing effort is not species specific but rather applies generally to a complex of reef‐associated species. Consequently, Red Snapper are discarded as bycatch when regulations prohibit their retention, and many of the discarded fish die from hook injury, barotrauma, or depredation. Methods: Here we developed a spatial population model of Red Snapper and the multispecies fishery that captures them in the southeast U.S. Atlantic. We then simulated and compared 25 different management measures that fall broadly into the categories of gear modifications, discard mortality mitigation, size limits, spatial approaches, or temporal approaches. Criteria for comparison address the management goals of decreasing dead discards, rebuilding the age structure, and increasing landings and spawning biomass. Result: We found that the most effective measures reduced fishing effort, either temporally or spatially, and that benefits could largely be obtained by focusing on the recreational fleet. Discard mortality mitigation (e.g., through use of descender devices) displayed a wide range in effectiveness depending on plausible levels of mortality reduction, but it addressed all management goals and in practice could be paired with other measures. A measure with restricted recreational effort combined with full retention of all fish caught showed the greatest potential to simultaneously rebuild the stock, increase landings, and eliminate dead discards. Conclusion: To end overfishing of Red Snapper as required by law, resource managers should reconsider the policy of unrestricted effort of the private recreational fleet to this multispecies fishery. The benefits of restricted effort would include increased catch rates, larger landed fish, and fewer dead discards. Impact statementWe used a spatial population model of Red Snapper in the U.S. Atlantic to compare various fishery management strategies. The most effective strategy restricted fishing effort of the private recreational fleet, with benefits that included increased abundance, increased catch rates, larger landed fish, and fewer dead discards. [ABSTRACT FROM AUTHOR]

Large‐amplitude internal solitary waves (ISWs) propagating eastward toward the Alboran Sea have long been known in the Strait of Gibraltar. New in‐situ data and satellite images evidence northwestward propagating ISWs. These waves are probably the reflection of the well‐known eastward propagating wave along the Moroccan shelf. A simple 2D‐vertical section, run with the compressible non‐hydrostatic Coastal and Regional Ocean COmmunity model, illustrates that the Moroccan slope is conducive to reflection of incident solitary waves of amplitude observed in the Strait of Gibraltar. A clear signature of these waves in the Tarifa high‐frequency sea level oscillations is depicted that paves the way to studies of the seasonality of ISWs in the Strait of Gibraltar with the long‐time series of sea level at Tarifa. The polarity of the reflected waves, observed with Acoustic Doppler Current Profilers/Conductivity‐Temperature‐Depth array moorings, presents a slight variability possibly due to the internal tide oscillations. The reflected ISWs arrives at the mooring location in phase with the peak of the internal tide. For the strongest tide, the pynocline approaches a critical point where the polarity of the ISWs might reverse. Plain Language Summary: The Strait of Gibraltar is known as a hotspot for internal solitary waves (ISWs) generation, which propagate eastward toward the Alboran Sea. A new in‐situ data set combined with satellite images reveal northwestward propagating ISWs in the Strait of Gibraltar. These waves are likely generated by the reflection of the eastward propagating waves on the Moroccan shelf as shown by simple numerical simulations. The reflected wave train, whose leading wave can be either an elevation or a depression wave, arrives at the observation site in phase with the internal tide trough (maximum sinking of the isopycnals). In strong spring tides, the interface depth approaches the critical point, which allows the possibility of change of wave‐polarity, that is, depression versus elevation, thus explaining the double nature of the observed leading waves. Signatures of the reflected waves have been also identified in the high‐frequency sea level oscillations at Tarifa. This study paves the way for long‐term observations of ISWs in the Strait of Gibraltar. Key Points: Northwestward propagating internal solitary waves (ISWs) suspected to be reflections are evidenced in the Strait of GibraltarA simple numerical approach illustrates that incident ISWs of amplitude observed in the area can reflect on the Moroccan slopeHigh‐frequency sea level oscillations at Tarifa reveal a signature consistent with the observed ISWs [ABSTRACT FROM AUTHOR]

Background: Acute myeloid leukemia (AML) with myelodysplasia‐related characteristics is a heterogeneous subset of AML that has been challenged throughout the history of myeloid malignancies classifications, considered to have similar outcomes as intermediate‐ or adverse‐risk AML depending on the subgroup. However, little is known about the fate of these patients in refractory or relapsed situation (R/R) after first line therapy. Methods: A large series of R/R AML patients, recorded in the French DATAML registry, have received either intensive chemotherapy (ICT), azacitidine (AZA) as single agent, or best supportive care (BSC). A cohort of 183 patients (median age 63‐year‐old) with what was called at the time AML‐MRC has been explored, and data are reported here. Results: Patient status was refractory for 93, while 90 had relapsed. Respectively, 88, 34, and 61 were included in the three treatment arms. The median OS of the whole cohort was 4.2 months (95%CI: 3.1–5.6) with a mean 1‐year overall survival of 24% ± 3.2%. There was no significant survival difference between refractory and relapsed patients. The BSC group had overall a significantly worse outcome (p = 0.0001), and this remained true in both refractory (p = 0.01) and relapsed (p = 0.002) patients. Similar survivals were observed in both groups comparing ICT and AZA. Conclusions: These data, reporting about an ill‐explored population, indicate the poor prognosis of this condition where both ICT and AZA can be proposed. The latter, which was demonstrated here to be a feasible option, should be added to new targeted therapies. [ABSTRACT FROM AUTHOR]

CRK adaptor proteins are important for signal transduction mechanisms driving cell proliferation and positioning during vertebrate central nervous system development. Zebrafish lacking both CRK family members exhibit small, disorganized retinas with 50% penetrance. The goal of this study was to determine whether another adaptor protein might functionally compensate for the loss of CRK adaptors. Expression patterns in developing zebrafish, and bioinformatic analyses of the motifs recognized by their SH2 and SH3 domains, suggest NCK adaptors are well‐positioned to compensate for loss of CRK adaptors. In support of this hypothesis, proteomic analyses found CRK and NCK adaptors share overlapping interacting partners including known regulators of cell adhesion and migration, suggesting their functional intersection in neurodevelopment. [ABSTRACT FROM AUTHOR]

Objective: Severe subglottic stenosis develops as a response to intubation in 1% of the >200,000 neonatal intensive care unit infants per year and may require laryngotracheal reconstruction (LTR) with autologous hyaline cartilage. Although effective, LTR is limited by comorbidities, severity of stenosis, and graft integration. In children, there is a significant incidence of restenosis requiring revision surgery. Tissue engineering has been proposed to develop alterative grafting options to improve outcomes and eliminate donor‐site morbidity. Our objective is to engineer a decellularized, channel‐laden xenogeneic cartilage graft, that we deployed in a proof‐of‐concept, neonatal porcine LTR model. Methods: Meniscal porcine cartilage was freeze–thawed and washed with pepsin/elastase to decellularize and create microchannels. A 6 × 10‐mm decellularized cartilage graft was then implanted in 4 infant pigs in an anterior cricoid split. Airway patency and host response were monitored endoscopically until sacrifice at 12 weeks, when the construct phenotype, cricoid expansion, mechanics, and histomorphometry were evaluated. Results: The selective digestion of meniscal components yielded decellularized cartilage with cell‐size channels. After LTR with decellularized meniscus, neonatal pigs were monitored via periodic endoscopy observing re‐epithelization, integration, and neocartilage formation. At 12 weeks, the graft appeared integrated and exhibited airway expansion of 4 mm in micro‐CT and endoscopy. Micro‐CT revealed a larger lumen compared with age‐matched controls. Finally, histology showed significant neocartilage formation. Conclusion: Our neonatal porcine LTR model with a decellularized cartilage graft is a novel approach to tissue engineered pediatric LTR. This pilot study sets the stage for "off‐the‐shelf" graft procurement and future optimization of MEND for LTR. Level of Evidence: NA Laryngoscope, 134:807–814, 2024 [ABSTRACT FROM AUTHOR]

We herein report the first use of thiosquaramides as polymerization catalysts, which are shown to be effective for the controlled ROP of lactide in the presence of an alcohol source and NEt3. Comparison of their catalytic performances with the less acidic squaramides are also discussed. The observed catalytic activity of variously N‐substituted thiosquaramides suggest that a balanced NH Brønsted acidity is required for optimal performance. Most interestingly and rather unexpectedly, DFT‐supported calculations on thiosquaramide‐mediated lactide ROP catalysis suggest that secondary interactions between the thiosquaramide N‐substituents and the incoming lactide (presently of type C‐H⋅⋅⋅π‐arene) are crucial for catalytic activity. Though this type of interactions is quite common in organo‐catalysis, it has rarely been evidenced to play a key role in the area of organo‐catalyzed polymerizations. Such catalyst substituents/substrate interactions may well play a significant role in the catalytic performances of various other systems. [ABSTRACT FROM AUTHOR]

Evaluation of viability of new marine species in aquaculture systems is important to reduce negative social and environmental externalities, offering sustainable options for supply to fish markets. The objective was to describe the performance indicators of late juvenile yellowtail snapper (243.2 ± 57.1 mean weight of total fish) in an experimental recirculating aquaculture system (RAS) system supplied by seawater to determine its pre‐feasibility. Two densities were stocked, D1: 10 fish/tank (2.6 kg/m3, mean weight 258.3 ± 57 g) and D2: 20 fish/tank (4.7 kg/m3, mean weight 235.6 ± 55.9 g). Fish were fed to satiety with an extruded diet containing 46% crude protein and 12% crude lipid. After 238 days, survival rate was 93% for both densities, and mean weight and biomass were 534.5 ± 19.4 g and 5.7 kg/m3 for D1, and 606.9 ± 34.5 g and 10 kg/m3 for D2. Final feed conversion rate (3.13 for D1 and 3.03 for D2) did not differ significantly between densities. The exponents (b) of the length–weight relationship calculated for D1 (3.15) versus D2 (3.10) and condition factor (2.12 ± 0.07 for D1, and 2.03 ± 0.04 for D2) demonstrated a higher condition factor than those of their wild counterparts. This study is one of the few reports on this species' performance in late juvenile stage in RAS. [ABSTRACT FROM AUTHOR]

The relationship between extractant stereochemistry and their extraction performance has only poorly been established. In order to address a part of this concern, we investigated the Pu(IV) liquid‐liquid extraction (LLE) by using the N,N‐di‐(2‐ethylhexyl)butyramide (DEHBA), as well as those of its position isomers. DEHBA (ββ‐isomer) and N‐(2‐ethylhexyl)‐N‐(oct‐3‐yl)butyramide (EHOBA or αβ‐isomer) were synthesized as a mixture of stereoisomer or stereoenriched (R,S)‐ and (S,S)‐diastereoisomers, and were all assessed for PuIV LLE. The results showed that both the position and the stereoisomerism of the aliphatic substituents affect PuIV complexation and extraction. We found that Pu extraction is lowered by factor 2 to 4 when the ethyl branching group is closer to the complexing site. UV‐vis spectroscopy showed that this extraction decrease was affected by steric hindrance inducing a deprivation of Pu inner sphere complex. Effect of stereoisomerism is highlighted for branching closer to the complexing site (α‐position). Enantiopure DEHBA stereoisomers provided similar Pu extraction, whereas a slight decrease could be noticed for the more cluttered stereoenriched (αβ)‐isomers, which was also concomitant with a smaller population of inner sphere complex. In contrast, the stereoisomers mixture led to a strong decrease of Pu extraction because of an antagonistic association in the mixed complexes. [ABSTRACT FROM AUTHOR]

Background: Long‐term follow‐up (LTFU) clinics have been developed but only some childhood cancer survivors (CCS) attend long‐term follow‐up (LTFU). Objective: To identify factors that influence LTFU attendance. Methods: Five‐year CCS treated for a solid tumor or lymphoma in Gustave Roussy before 2000, included in the FCCSS cohort (French Childhood Cancer Survivor Study), aged >18 years and alive at the date of the LTFU Clinic opening (January 2012) were invited to a LTFU visit. Factors associated with attendance at the LTFU clinic between 2012 and 2020 were estimated using logistic regression analyses. Analyses included different types of factors: clinical (tumor characteristics, cancer treatments, late effects), medical (medical expenses were used as a proxy of survivor's health status), social (deprivation index based on census‐tract data relating to income, educational level, proportion of blue‐collar workers, and unemployed people living in the area of residence), and spatial (distance to the LTFU clinic). Results: Among 2341 CCS contacted (55% males, mean age at study, 45 years; SD ± 10 years; mean age at diagnosis, 6 years; SD ± 5 years), 779 (33%) attended at least one LTFU visit. Initial cancer‐related factors associated with LTFU visit attendance were: treatment with both radiotherapy and chemotherapy (odds ratio [OR], 4.02; 95% CI, 2.11–7.70), bone sarcoma (OR, 2.43; 95% CI, 1.56–3.78), central nervous system primitive tumor (OR, 1.65; 95% CI, 1.02–2.67), and autologous hematopoietic cell transplant (OR, 2.07; 95% CI, 1.34‐3.20). Late effects (OR, 1.70; 95% CI, 1.31–2.20), highest medical expenses (OR, 1.65; 95% CI, 1.22–2.22), living in the most advantaged area (OR vs. the most deprived area = 1.60; 95% CI, 1.15–2.22), and shorter distance from LTFU care center (<12 miles) also increased attendance. Conclusions: Patients who are apparently healthy as well as socially disadvantaged and living far away from the center are less likely to attend LTFU care. Plain Language Summary: Among 2341 adult childhood cancer survivors contacted between 2012 and 2020, 33% attended at least one long‐term follow‐up visit.Clinical factors related to attendance were multimodal treatment of first cancer (combining chemotherapy and radiotherapy), stem cell transplant, type of diagnosis (bone tumor and central nervous system primitive tumor), late effects (at least one disease among second malignancy, heart disease, or stroke), and highest medical expenses.In addition, the study identified social and spatial inequalities related to attendance, with independent negative effects of distance and social deprivation on attendance, even though the medical costs related to the long‐term follow‐up examinations are covered by the French social security system. Among 2341 adult childhood cancer survivors contacted between 2012 and 2020, 33% attended at least one long‐term follow‐up visit. Clinical factors related to attendance were multimodal treatment of first cancer (combining chemotherapy and radiotherapy), stem cell transplant, type of diagnosis (bone tumor and central nervous system primitive tumor), late effects (at least one disease among second malignancy, heart disease, or stroke ascertained from a physician or a medical record) and highest medical expenses (used as a proxy of survivor's health status in adulthood). In addition, the study identified social and spatial inequalities related to attendance, with independent negative effects of distance and social deprivation on attendance, even though the medical costs related to the long‐term follow‐up examinations are covered by the French social security system. [ABSTRACT FROM AUTHOR]

Root exudation is involved in the recruitment of beneficial microorganisms by trophic relationships and/or signalling pathways. Among beneficial microorganisms, Plant Growth‐Promoting Rhizobacteria (PGPR) are known to improve plant growth and stress resistance. These interactions are of particular importance for species that do not interact with mycorrhizal fungi, such as rapeseed (Brassica napus L.) and camelina (Camelina sativa (L.) Crantz). However, heat stress is known to have a quantitative and qualitative impact on root exudation and could affect the interactions between plants and PGPR. We aimed to analyse the effects of PGPR inoculation on root morphology and exudation in rapeseed and camelina at the reproductive stage. The modulation of the effects of these interactions under heat stress was also investigated. The plants were inoculated twice at the reproductive stage with two different Pseudomonas species and were exposed to heat stress after the second inoculation. In non‐stressing conditions, after bacterial inoculation, rapeseed and camelina exhibited two contrasting behaviours in C root allocation. While rapeseed plants seemed to suffer from the interactions with the bacteria, camelina plants appeared to control the relationship with the PGPR by modifying the composition of their root exudates. Under heat stress, the plant‐PGPR interaction was unbalanced for rapeseed, for which the C allocation strategy is mainly driven by the C cost from the bacteria. Alternatively, camelina plants prioritized C allocation for their own above‐ground development. This work opens up new perspectives for understanding plant‐PGPR interactions, especially in an abiotic stress context. [ABSTRACT FROM AUTHOR]

Increasing demand for glutaminase (GLS) due to high rates of glutamine metabolism is considered one of the hallmarks of malignancy. In parallel, cancer antigen 125 (CA‐125) is a commonly used ovarian tumor marker. This study aimed to compare the roles of GLS and CA‐125 in distinguishing between benign and malignant ovarian tumors. The research was conducted as a comparative study, enrolling 156 patients with ovarian tumors. Preoperative serum CA‐125 and GLS levels were analyzed to evaluate their effectiveness in distinguishing between benign and malignant ovarian tumors. The results revealed that the mean levels of CA‐125 and GLS were significantly higher in malignant ovarian tumors compared with benign ones (389.54 ± 494.320 vs. 193.15 ± 529.932 (U/mL) and 17.37 ± 12.156 vs. 7.48 ± 4.095 (μg/mL), respectively). The CA‐125 and GLS cutoff points of 108.2 U/mL and 18.32 μg/mL, respectively, were associated with malignant ovarian tumors. Multivariate analyses showed that GLS had higher predictive capabilities compared with CA‐125 (odds ratio 9.4 vs. 2.1). The accuracy of using GLS combined with CA‐125 was higher than using CA‐125 alone (73.1% vs. 68.8%). In conclusion, higher levels of CA‐125 and GLS are associated with malignant ovarian tumors. GLS outperforms CA‐125 in distinguishing between benign and malignant ovarian tumors. The combination of GLS and CA‐125 demonstrated improved accuracy for distinguishing benign and malignant ovarian tumors when compared with using CA‐125 alone. [ABSTRACT FROM AUTHOR]

In the context of public health crises such as the COVID‐19 pandemic, it is essential that individuals cooperate by complying with preventive measures (e.g., wearing a mask). The current research examines how high trust in close others is linked to less cooperation—that is, less compliance with measures—and thus, undermines collective interests. Specifically, we test whether individuals are less willing to comply with preventive measures when interacting with close others they trust. We conducted two experiments in which participants read a vignette depicting a social interaction with either close others (e.g., family) or strangers. Participants had to report the extent to which they would (1) trust the other people in the situation and (2) comply with the mask wearing and physical distancing measures during this interaction. In both experiments, we find that when individuals are considering an interaction with close others, they report experiencing higher trust which is then linked to lower compliance with preventive measures. In Experiment 2, we further demonstrate that participants report less compliance with preventive measures around close others, even when they perceive non‐compliance with the measures as morally "wrong". Our findings shed light on the challenges that compliance with preventive measures poses during social interactions in a context of high trust. [ABSTRACT FROM AUTHOR]

Objective: Patients with spondyloarthritis (SpA) often present with microscopic signs of gut inflammation, a risk factor for progressive disease. We investigated whether mucosal innate‐like T cells are involved in dysregulated interleukin‐23 (IL‐23)/IL‐17 responses in the gut‐joint axis in SpA. Methods: Ileal and colonic intraepithelial lymphocytes (IELs), lamina propria lymphocytes (LPLs), and paired peripheral blood mononuclear cells (PBMCs) were isolated from treatment‐naive patients with nonradiographic axial SpA with (n = 11) and without (n = 14) microscopic gut inflammation and healthy controls (n = 15) undergoing ileocolonoscopy. The presence of gut inflammation was assessed histopathologically. Immunophenotyping of innate‐like T cells and conventional T cells was performed using intracellular flow cytometry. Unsupervised clustering analysis was done by FlowSOM technology. Serum IL‐17A levels were measured via Luminex. Results: Microscopic gut inflammation in nonradiographic axial SpA was characterized by increased ileal intraepithelial γδ‐hi T cells, a γδ‐T cell subset with elevated γδ‐T cell receptor expression. γδ‐hi T cells were also increased in PBMCs of patients with nonradiographic axial SpA versus healthy controls and were strongly associated with Ankylosing Spondylitis Disease Activity Score. The abundance of mucosal‐associated invariant T cells and invariant natural killer T cells was unaltered. Innate‐like T cells in the inflamed gut showed increased RORγt, IL‐17A, and IL‐22 levels with loss of T‐bet, a signature that was less pronounced in conventional T cells. Presence of gut inflammation was associated with higher serum IL‐17A levels. In patients treated with tumor necrosis factor blockade, the proportion of γδ‐hi cells and RORγt expression in blood was completely restored. Conclusion: Intestinal innate‐like T cells display marked type 17 skewing in the inflamed gut mucosa of patients with nonradiographic axial SpA. γδ‐hi T cells are linked to intestinal inflammation and disease activity in SpA. [ABSTRACT FROM AUTHOR]

Recently, PROTEVS GIB20 experiment was performed in the Strait of Gibraltar. Part of this experiment was dedicated to observe the high frequency dynamics near Camarinal Sill (CS), considered as a mixing hotspot in the region. Mooring lines equipped with current profilers and temperature/salinity probes provided data which evidence two dynamical regimes depending on the tidal current intensity; in neap tide floods, local internal hydraulic control is never observed over CS while in spring tide, local internal hydraulic control depicts a tide‐dependent and spatially variable pattern. In spring tide floods, measurements revealed the development of a hydraulic jump over the sill and its advection on the lee side. Cross sill sections with CTD casts and acoustic images confirmed this dynamics and depicted a well developed hydraulic jump on the eastern flank of the sill during spring tide ebbs. The north‐south and temporal variability of the internal hydraulics was analyzed from several zonal sections over CS, the mean topographic feature of the Strait of Gibraltar. We highlighted a complex series of local hydraulic jumps constrained by topography and a significant north‐south variability. The spatiotemporal variability of local hydraulics questions the two dimensional representation of the exchange flow in the Strait of Gibraltar. During neap tide flood, the dynamics of the Mediterranean outflow was investigated from a fixed station. We imaged the development of instabilities at the interface between Atlantic and Mediterranean waters jointly with the generation of much thicker billows deeper. Finally, we discuss our findings in relation to other straits dynamics. Plain Language Summary: A recent field experiment, PROTEVS GIB20, was performed in the Strait of Gibraltar in October 2020. This experiment was partly designed to observe the turbulent dynamics of the flow in this region. The Strait of Gibraltar presents an important topographic sill, Camarinal Sill (CS), where the current reaches its maximum value enhancing the turbulent processes. Understanding the processes developed at this place is of crucial importance to better quantify the induced mixing of the Atlantic/Mediterranean water masses along their journey in the Strait. With acoustic, temperature/salinity, and current measurements we analyzed and described the spatio‐temporal variability of the turbulent processes as hydraulic jumps, shear instabilities (like Kelvin‐Helmholtz billows) and turbulent gravity current. Finally, we tackled the question of the spatiotemporal variability of the flow near CS and demonstrated that it is far from two‐dimensional. Neap tide dynamics reveals large instabilities in the Mediterranean Outflow that may induced significant mixing. All these turbulent processes induce significant mixing between Atlantic and Mediterranean waters and thus influence heat and salinity fluxes through the Strait of Gibraltar. Key Points: A recent field experiment highlights a series of Hydraulic Jumps during spring tide floods around the main sill of the Strait of GibraltarHigh frequency observations reveal local hydraulic control during spring tide ebbs generating westward propagating internal solitary wavesAt neap tide, highly turbulent flows develop, forming an intense bottom boundary layer and large overturns on the sill slope [ABSTRACT FROM AUTHOR]

Previous studies of MS have reported a CR rate of 45%-70% after IC and median OS of 12.8-15.9 months.[[11], [13]] Our multi-variate Cox model showed that an age >65 years, a BM blast rate >=20% and patients with refractory or relapsing disease were independently associated with mortality. Myeloid sarcoma (MS) accounts for 3%-8% acute myeloid leukaemia (AML) cases and is characterized by an extramedullary proliferation of myeloid blast cells forming a solid tumour,[1] with or without bone marrow (BM) involvement.[[2]] A history of other myeloid neoplasms can precede MS occurrence.[4] MS literature is scarce with mostly heterogeneous cohorts mixing primary MS with extramedullary AML relapses. Patients with extramedullary AML relapses and with no MS at their first AML diagnosis were excluded, but patients with a history of MDS/MPN were eligible. The median OS was not reached for patients with a bone marrow (BM) blast rate <20% versus 24 months for patients with a BM blast rate >=20%. [Extracted from the article]

Purpose: This study assessed sustainable return to work (SRTW) of breast cancer survivors (BCS). Methods: We used data from the prospective French cohort, CANTO. We included 1811 stage I–III BCS who were <57 years old and employed at the moment of diagnosis and working 2 years after diagnosis. Using logistic regression, we investigated the role of clinical, health and socio‐economic factors, and the work environment on SRTW 3 years after diagnosis. We compared having any sick leave with having worked continuously and being unemployed to having worked continuously between 2 and 3 years after diagnosis. Results: Overall, 77% (n = 1395) worked continuously after return to work (RTW). Out of the other 416 BCS, 66% had any sick leave period, 33% had been unemployed, 4% had an early retirement, 2% a disability and 1% another status (multiple situations possible). Being on sick leave was associated with age > 50 (OR = 0.59; 95%CI = 0.43–0.82), stage III (2.56; 1.70–3.85), tumour subtype HR+/HER2+ (0.61; 0.39–0.95), severe fatigue (1.45; 1.06–1.98), workplace accommodations (1.63; 1.14–2.33) and life priorities (0.71; 0.53–0.95). Unemployment was associated with age > 50 (0.45; 0.29–0.72), working in the public sector (0.31; 0.19–0.51), for a small company (3.00; 1.74–5.20) and having a fixed‐term contract (7.50; 4.74–11.86). Conclusions: A high number of BCS have periods of sick leave or unemployment after RTW. The determinants differ between sick leave and unemployment. Implications for cancer survivors: BCS need to be supported even after RTW, which should be regarded as a process. [ABSTRACT FROM AUTHOR]