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Upon repeating the experiments, the authors found that the number of equivalents needed to elicit a change in the absorption and emission spectra was higher than those originally reported in the manuscript, and the ratiometric response was lower. (e) absorbance and (f) fluorescence spectral changes of 1 (20 M) upon Na3PO4 titration, inset: (e) ratio of absorbance at 447 and 358 nm as a function of Pi concentration; (f) ratio of fluorescence intensity at 531 and 450 nm as a function of Pi concentration. gl We would like to replace the sentences "The absorption ratio (A427/A357) increased gradually from 0.04 to 1.64, and the fluorescence ratio (F532/F453) showed a sigmoid dependency on the Pi concentration, resulting in a 91-fold enhancement (Figure e,f, inset). This indicated a quantitative detection of Pi by fluorescence ratiometry in the 25 to 75 equivalent ranges." with " The absorption ratio (A427/A357) increased gradually from 0.062 to 0.819, and the fluorescence ratio (F532/F453) showed a sigmoid dependency on the Pi concentration, resulting in a 9-fold enhancement (Figure e,f, inset). [Extracted from the article]

A chemodosimeter was designed to function as a highly selective phosphate ion (Pi) sensor, showing a 91-fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase-catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells. [ABSTRACT FROM AUTHOR]

A chemodosimeter was designed to function as a highly selective phosphate ion (Pi) sensor, showing a 91-fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase-catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells. [ABSTRACT FROM AUTHOR]