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Adverse weather has been shown to be spatially and temporally variable across high‐latitude locations. The current study provides a unique investigation of limited surface visibility time periods at five coastal Greenland locations from 1979 to 2018 and identifies the coincident adverse weather, as well as the local and large‐scale atmospheric conditions during limited surface visibility time periods. Locations on the east coast of Greenland have the largest percentage of hours with limited visibility each month with maxima during February. Western and southern coastal locations have fewer occurrences of limited visibility. Warm‐season maxima are present in the northern locations, while warm‐season minima occur at all other locations. Fog is reported during each month at all five stations, however, a substantial increase of hours occurs at the northern and eastern stations during the typical melt season on Greenland. There is a seasonal difference in the percent of limited visibility hours linked to precipitation with a minimum in the warm season and maximum across the cold season. Limited visibility attributed to precipitation generally has the largest percentage of hours each month except for the northern and eastern locations during the melt season. The location and number of cyclonic circulations, as well as the spatial scale of troughs, across northeastern Canada and the North Atlantic Ocean greatly influence the adverse weather linked to prolonged limited surface visibility events. Limited visibility events at Thule, Greenland during both the warm and cold seasons are largely influenced by the presence and intensity of a cyclone over northeastern Canada. At Danmarkshavn, Greenland limited visibility events in both seasons generally have a cyclone positioned to the east, an area of higher pressure over Greenland and a cyclone positioned to the west of Greenland. [ABSTRACT FROM AUTHOR]

The repair of double‐strand DNA breaks (DSBs) by homologous recombination is crucial in the maintenance of genome integrity. While the key role of the Mre11‐Rad50‐Nbs1 (MRN) complex in repair is well known, hSSB1 (SOSSB and OBFC2B), one of the main components of the sensor of single‐stranded DNA (SOSS) protein complex, has also been shown to rapidly localize to DSB breaks and promote repair. We have previously demonstrated that hSSB1 binds directly to Nbs1, a component of the MRN complex, in a DNA damage‐independent manner. However, recruitment of the MRN complex has also been demonstrated by an interaction between Integrator Complex Subunit 3 (INTS3; also known as SOSSA), another member of the SOSS complex, and Nbs1. In this study, we utilize a combined approach of in silico, biochemical, and functional experiments to uncover the molecular details of INTS3 binding to Nbs1. We demonstrate that the forkhead‐associated domain of Nbs1 interacts with INTS3 via phosphorylation‐dependent binding to INTS3 at Threonine 592, with contributions from Serine 590. Based on these data, we propose a model of MRN recruitment to a DSB via INTS3. [ABSTRACT FROM AUTHOR]

Meteorological drivers of groundwater recharge for spring (February–June), fall (October–January), and recharge‐year (October–June) recharge seasons were evaluated for northern New England and upstate New York from 1989 to 2018. Monthly groundwater recharge was computed at 21 observation wells by subtracting the water levels at the end of each month from the level of the previous month; only positive monthly values were used to compute seasonal recharge. Precipitation, temperature, sea‐level pressure, 500‐mb geopotential heights, and various teleconnection indices were tested as explanatory variables for the interannual variability of recharge using random forest machine learning models. Precipitation within recharge seasons was positively correlated with groundwater recharge for most wells in all seasons. In general, whilst groundwater recharge in the study area was generally highest during the months of March and April, October precipitation was an important month for explaining the interannual groundwater recharge variability. This is likely because the variability in recharge in October may be high or low for given years. Sea‐level pressure and 500‐mb heights were typically inversely correlated with groundwater recharge during the recharge‐year and fall recharge seasons, as higher sea‐level pressure and heights are usually associated with clearer skies and less precipitation. The North Atlantic Oscillation, Pacific‐North American pattern, and Pacific Decadal Oscillation teleconnections affected groundwater recharge differently by well and season. The influence of groundwater recharge on minimum daily streamflows during the subsequent summer/fall was also analysed. Summer precipitation was the most important explanatory variable for study streams whilst groundwater recharge and summer air temperature were significant variables for a few streams. [ABSTRACT FROM AUTHOR]

Through an expansive international effort that involved data collection on 12 small‐angle X‐ray scattering (SAXS) and four small‐angle neutron scattering (SANS) instruments, 171 SAXS and 76 SANS measurements for five proteins (ribonuclease A, lysozyme, xylanase, urate oxidase and xylose isomerase) were acquired. From these data, the solvent‐subtracted protein scattering profiles were shown to be reproducible, with the caveat that an additive constant adjustment was required to account for small errors in solvent subtraction. Further, the major features of the obtained consensus SAXS data over the q measurement range 0–1 Å−1 are consistent with theoretical prediction. The inherently lower statistical precision for SANS limited the reliably measured q‐range to <0.5 Å−1, but within the limits of experimental uncertainties the major features of the consensus SANS data were also consistent with prediction for all five proteins measured in H2O and in D2O. Thus, a foundation set of consensus SAS profiles has been obtained for benchmarking scattering‐profile prediction from atomic coordinates. Additionally, two sets of SAXS data measured at different facilities to q > 2.2 Å−1 showed good mutual agreement, affirming that this region has interpretable features for structural modelling. SAS measurements with inline size‐exclusion chromatography (SEC) proved to be generally superior for eliminating sample heterogeneity, but with unavoidable sample dilution during column elution, while batch SAS data collected at higher concentrations and for longer times provided superior statistical precision. Careful merging of data measured using inline SEC and batch modes, or low‐ and high‐concentration data from batch measurements, was successful in eliminating small amounts of aggregate or interparticle interference from the scattering while providing improved statistical precision overall for the benchmarking data set. [ABSTRACT FROM AUTHOR]

Introduction: Venous air embolization (VAE) from IV lines is a risk with IV contrast administration. The incidence of VAE ranges from 7% to 23%. While life‐threatening VAE is rare, there is the potential risk of serious cerebrovascular events from paradoxical systemic air embolization (SAE) in patients with congenital intracardiac shunts. CT coronary angiogram (CTCA) hypothetically carries a higher risk of VAE as it requires dual injections as compared to single bolus dose contrast used in CT chest (CTC). The aim of this study was to assess the risk of VAE using CTCA as compared to conventional CTC. The incidence of cardiac shunts and their association with paradoxical SAE were also investigated. Methods: A retrospective study was conducted at a tertiary hospital. Adult patients undergoing CTCA and CTC over a 6‐month period in 2017 were included. Images were reviewed on PACS for the presence of VAE. Electronic medical records were interrogated for evidence of neurological sequelae or requiring neurological imaging (CTB/MRIB) within 1 month of the initial imaging. Results: 508 patients were included, 408 underwent CTCA and 100 underwent CTC. The VAE incidence in CTCA was 24% (94 patients) and 10% in the CTC (10 patients). This was statistically significant. Among the CTCA group, 36% (108 patients) had an intracardiac shunt. No statistically significant difference in VAE incidence was identified in CTCA patients with shunts vs without shunts. There was no incidence of SAE in both groups. Conclusion: VAE is more common with CTCA than conventional CTC. There were no cases of paradoxical SAE found. [ABSTRACT FROM AUTHOR]

Rationale: Matrix-assisted laser desorption ionisation with mass spectrometry imaging (MSI) has seen rapid development in recent years and as such is becoming an important technique for the mapping of biomolecules from the surface of tissues. One key area of development is the optimisation of analyte extraction by using modified matrices or mixes of common ones. Methods: A series of serial sections were prepared for lipid MSI by either dry coating (sublimation) or by wet spray application of several matrices. These samples were then evaluated for analyte extraction, delocalisation and dynamic range. Results: We have shown that the spraying and sublimation methods of matrix application can be used complementarily. This creates large datasets, with each preparation method applied narrowly and then interpreted as a 'fraction' of the whole. Once combined, the dynamic range is significantly increased. We have dubbed this technique 'matrix phase fractionation'. Conclusions: We have found that, by utilising matrix phase fractionation for the detection of lipids in brain tissue, it is possible to create a significantly more comprehensive dataset than would otherwise be possible with traditional 'single-run' workflows. [ABSTRACT FROM AUTHOR]

Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck ( Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies. [ABSTRACT FROM AUTHOR]

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Isoprene emission is an important mechanism for improving the thermotolerance of plant photosystems as temperatures increase. In this study, we measured photosynthesis and isoprene emission in trees along an urban-rural gradient that serves as a proxy for climate change, to understand daily and seasonal responses to changes in temperature and other environmental variables. Leaf-level gas exchange and basal isoprene emission of post oak (Quercus stellata) and sweet gum ( Liquidambar styraciflua) were recorded at regular intervals over an entire growing season at urban, suburban, and rural sites in eastern Texas. In addition, the temperature and atmospheric carbon dioxide concentration experienced by leaves were experimentally manipulated in spring, early summer, and late summer. We found that trees experienced lower stomatal conductance and photosynthesis and higher isoprene emission, at the urban and suburban sites compared to the rural site. Path analysis indicated a daily positive effect of isoprene emission on photosynthesis, but unexpectedly, higher isoprene emission from urban trees was not associated with improved photosynthesis as temperatures increased during the growing season. Furthermore, urban trees experienced relatively higher isoprene emission at high CO2 concentrations, while isoprene emission was suppressed at the other sites. These results suggest that isoprene emission may be less beneficial in urban, and potentially future, environmental conditions, particularly if higher temperatures override the suppressive effects of high CO2 on isoprene emission. These are important considerations for modeling future biosphere-atmosphere interactions and for understanding tree physiological responses to climate change. [ABSTRACT FROM AUTHOR]

Background and objective This study aimed to evaluate the involvement of airway cross-sectional area and shape, and functional residual capacity ( FRC), in the genesis of obstructive sleep apnoea ( OSA) in patients with supine-predominant OSA. Methods Three groups were recruited: (i) supine OSA, defined as a supine apnoea-hyponoea index ( AHI) at least twice that of the non-supine AHI; (ii) rapid eye movement ( REM) OSA, defined as REM AHI at least twice the non- REM AHI and also selected to have supine AHI less than twice that of the non-supine AHI (i.e. to be non-positional); and (iii) no OSA, defined as an AHI less than five events per hour. The groups were matched for age, gender and body mass index. Patients underwent four-dimensional computed tomography scanning of the upper airway in the supine and lateral decubitus positions. FRC was measured in the seated, supine and lateral decubitus positions. Results Patients with supine OSA demonstrated a significant decrease in FRC of 340 mL ( P = 0.026) when moving from the lateral to supine position compared to controls with no OSA, and REM OSA patients. We found no differences between groups in upper airway size and shape. However, all groups showed a significant change in airway shape with the velopharyngeal airway adopting a more elliptoid shape (with the long axis laterally oriented), with reduced anteroposterior diameter in the supine position. Conclusions A fall in FRC when moving lateral to supine in supine OSA patients may be an important triggering factor in the generation of OSA in this patient group. [ABSTRACT FROM AUTHOR]

Outcomes for melanoma patients with stage III disease differ widely even within the same subcategory. Molecular signatures that more accurately predict prognosis are needed to stratify patients according to risk. Proteomic analyses were used to identify differentially abundant proteins in extracts of surgically excised samples from patients with stage IIIc melanoma lymph node metastases. Analysis of samples from patients with poor (n = 14, <1 yr) and good (n = 19, >4 yr) survival outcomes identified 84 proteins that were differentially abundant between prognostic groups. Subsequent selected reaction monitoring analysis verified 21 proteins as potential biomarkers for survival. Poor prognosis patients are characterized by increased levels of proteins involved in protein metabolism, nucleic acid metabolism, angiogenesis, deregulation of cellular energetics and methylation processes, and decreased levels of proteins involved in apoptosis and immune response. These proteins are able to classify stage IIIc patients into prognostic subgroups (P < 0.02). This is the first report of potential prognostic markers from stage III melanoma using proteomic analyses. Validation of these protein markers in larger patient cohorts should define protein signatures that enable better stratification of stage III melanoma patients. [ABSTRACT FROM AUTHOR]

ABSTRACT The American woodcock ( Scolopax minor) population has declined since the late 1960s across its range and is now considered a species of special concern. Research on woodcock habitat use during migration and migratory routes through the Central Flyway has been limited. We assessed woodcock phenology, estimated density, and nocturnal habitat use in fields on public lands in the lower Mississippi Alluvial Valley portion of Arkansas during November and December of 2010 and 2011. We used all-terrain vehicles to survey woodcock along transects in 67 fields of 8 field types. We analyzed data using hierarchical distance sampling. We detected woodcock from the first week in November through the third week in December but in low numbers. We did not detect woodcock in millet or rice fields, whereas woodcock had the highest estimated densities in unharvested soybeans. All other crop type-post-harvest management combinations had low woodcock densities. We did not detect woodcock in fields <8 ha or >40 ha. Woodcock in the lower Mississippi Alluvial Valley may benefit from management for unharvested soybean fields of moderate size (approx. 8-40 ha). © 2014 The Wildlife Society. [ABSTRACT FROM AUTHOR]

Introduction The aim of this study was to evaluate the dynamic volumetric CT in the assessment of the paediatric airway. Methods Ethics board approval was obtained for this retrospective review. Eight infants (median age 6 months, range 3 weeks to 1 year, 50% female) at a tertiary paediatric centre with complex clinical respiratory presentation underwent volumetric CT assessment of their airways. The entire lungs were examined over 1-2 respiratory cycles. In four patients, intravenous contrast was administered to assess for vascular airway compression. The patients were not intubated. CT findings were correlated with bronchography and bronchoscopy, where available. Results Two patients had diffuse tracheobronchomalacia associated with chronic lung disease. One patient demonstrated focal severe cervical tracheomalacia. One patient had a double aortic arch causing fixed narrowing with superimposed malacia of the distal trachea. Four patients had normal airways; one with chronic lung disease, one demonstrating air trapping. CT findings were concordant with bronchography (one case) and bronchoscopy (four cases) in all but one ( CT negative, bronchoscopy positive) but did not alter patient management. Conclusion The assessment of the paediatric airway, and in particular for tracheobronchomalacia, is difficult. Assessment with bronchography, bronchoscopy, helical CT and MR have issues with reliability, intubation, intratracheal/bronchial contrast administration and ionising radiation. Volumetric CT assesses the entire central airway in children at much lower radiation dose compared with previous dynamic CT imaging. This non-invasive, rapid assessment obviates the need for patient cooperation and enables evaluation of extratracheal intrathoracic structures. Volumetric CT enables four-dimensional assessment for paediatric tracheobronchomalacia without intubation or patient cooperation and at low radiation dose. [ABSTRACT FROM AUTHOR]

Summary Mycobacterium strain NBB4 was isolated on ethene as part of a bioprospecting study searching for novel monooxygenase (MO) enzymes of interest to biocatalysis and bioremediation. Previous work indicated that strain NBB4 contained an unprecedented diversity of MO genes, and we hypothesized that each MO type would support growth on a distinct hydrocarbon substrate. Here, we attempted to untangle the relationships between MO types and hydrocarbon substrates. Strain NBB4 was shown to grow on C2-C4 alkenes and C2-C16 alkanes. Complete gene clusters encoding six different monooxygenases were recovered from a fosmid library, including homologues of ethene MO ( etnABCD), propene MO ( pmoABCD), propane MO ( smoABCD), butane MO ( smoXYB1C1Z), cytochrome P450 (CYP153; fdx-cyp-fdr) and alkB ( alkB-rubA1-rubA2). Catabolic enzymes involved in ethene assimilation (EtnA, EtnC, EtnD, EtnE) and alkane assimilation (alcohol and aldehyde dehydrogenases) were identified by proteomics, and we showed for the first time that stress response proteins (catalase/peroxidase, chaperonins) were induced by growth on C2-C5 alkanes and ethene. Surprisingly, none of the identified MO genes could be specifically associated with oxidation of small alkanes, and thus the nature of the gaseous alkane MO in NBB4 remains mysterious. [ABSTRACT FROM AUTHOR]

Gamma-hydroxybutyrate (GHB) is a prescribed medication as well as a drug of abuse. Its detection in various matrices for in-field forensic scientists remains a challenge. We have developed an assay that uses aldo-keto reductase 7A2 (AKR7A2) for the specific determination of GHB in various drinks. AKR7A2 was purified using Ni-affinity chromatography. The Michaelis-Menten constant for the GHB oxidation reaction was 10 mM, and the minimum detection limit was 4 mM. Ethanol was not a substrate for AKR7A2. In a coupled reaction with NADP, phenazine methosulfate (PMS), and 2,6-dichlorophenolindophenol, various beverages (orange juice, milk, soda, and numerous alcoholic drinks) containing GHB turned from blue to light yellow. In a second coupled reaction where diaphorase replaced PMS, the presence of GHB also caused the expected change of color in various beers. [ABSTRACT FROM AUTHOR]

We propose a method to analyze family-based samples together with unrelated cases and controls. The method builds on the idea of matched case-control analysis using conditional logistic regression (CLR). For each trio within the family, a case (the proband) and matched pseudo-controls are constructed, based upon the transmitted and untransmitted alleles. Unrelated controls, matched by genetic ancestry, supplement the sample of pseudo-controls; likewise unrelated cases are also paired with genetically matched controls. Within each matched stratum, the case genotype is contrasted with control/pseudo-control genotypes via CLR, using a method we call matched-CLR (mCLR). Eigenanalysis of numerous SNP genotypes provides a tool for mapping genetic ancestry. The result of such an analysis can be thought of as a multidimensional map, or eigenmap, in which the relative genetic similarities and differences amongst individuals is encoded in the map. Once constructed, new individuals can be projected onto the ancestry map based on their genotypes. Successful differentiation of individuals of distinct ancestry depends on having a diverse, yet representative sample from which to construct the ancestry map. Once samples are well-matched, mCLR yields comparable power to competing methods while ensuring excellent control over Type I error. Copyright © 2010 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Background and objective: Vocal cord dysfunction (VCD) often masquerades as asthma and reports have suggested that up to 30% of patients with asthma may have coexistent VCD. Diagnosis of VCD is difficult, in part because it involves laryngoscopy which has practical constraints, and there is need for rapid non-invasive diagnosis. High speed 320-slice volume CT demonstrates laryngeal function during inspiration and expiration and may be useful in suspected VCD. Methods: Endoscopy and high resolution 320-slice dynamic volume CT were used to examine and compare laryngeal anatomy and movement in a case of subglottic stenosis and in a patient with confirmed VCD. Nine asthmatics with ongoing symptoms and suspected VCD also underwent 320-slice dynamic volume CT. Tracheal and laryngeal anatomy and movement were evaluated and luminal areas were measured. Reductions in vocal cord luminal area >40%, lasting for >70% duration of inspiration/expiration, were judged to be consistent with VCD. Results: Studies of subglottic tracheal stenosis validated anatomical similarities between endoscopy and CT images. Endoscopy and 320-slice volume CT also provided comparable dynamic images in a patient with confirmed VCD. A further nine patients with a history of severe asthma and suspected VCD were studied using CT. Four patients had evidence of VCD and the median reduction of luminal area during expiration was 78.2% (range 48.2–92.5%) compared with 10.4% (range 4.7–30%) in the five patients without VCD. Patients with VCD had no distinguishing clinical characteristics. Conclusions: Dynamic volume CT provided explicit images of the larynx, distinguished function of the vocal cords during the respiratory cycle and could identify putative VCD. The technique will potentially provide a simple, non-invasive investigation to identify laryngeal dysfunction, permitting improved management of asthma. [ABSTRACT FROM AUTHOR]

The School Health Index (SHI) is a self-assessment and planning tool that helps individual schools identify the strengths and weaknesses of their health policies and programs. To determine the percentage of US schools meeting the recommendations in the SHI, the present study analyzed data from the School Health Policies and Programs Study (SHPPS) 2000. The SHPPS 2000 data were collected through computer-assisted personal interviews with faculty and staff in a nationally representative sample of schools. The SHPPS 2000 questions were then matched to SHI items to calculate the percentage of schools meeting the recommendations in 4 areas: school health and safety policies and environment, health education, physical education and other physical activity programs, and nutrition services. Although schools nationwide are meeting a few SHI items in each of these areas, few schools are addressing the entire breadth of items. A more coordinated approach to school health would help schools reinforce health messages. (J Sch Health. 2006;76(2):57-66) [ABSTRACT FROM AUTHOR]

Provides information on a study that described school-level policies and practices on the general school environment, prevention of injuries, prohibition of tobacco use, alcohol use, drug use and violence in the United States. Methodology of the study; Results and discussion on the study; Conclusion.

This study examined demographic characteristics of tobacco-using high school students in the United States from 1991 through 1995. Data about cigarette smoking and smokeless tobacco use among adolescent were collected in 1991, 1993, and 1995 using the Youth Risk Behavior Survey, part of the Youth Risk Behavior Surveillance System implemented by the Centers for Disease Control and Prevention. Data indicated current smoking increased 26.5% from 1991 to 1995 with one-third [31.2% (+/- 1.7)] of ninth grade students and 38.2% (+/- 3.5) of 12th grade students reporting current smoking in 1995. Smokeless tobacco use remained stable with 11.4% (+/- 1.7) of all students and one-fourth [25.1% (+/- 3.0)] of White male students reporting smokeless tobacco use in 1995. Many students already have begun using tobacco before reaching high school. Thus, interventions should begin well before high school to prevent adolescents from using and becoming addicted to tobacco. [ABSTRACT FROM AUTHOR]

The article presents a study about the vertical distribution of attached marine algae off the coast of Malta. Specific details were observed on the characteristics of green algae in a vertical underwater cliff in Malta. Biomass decrease was noted relevant to the rapid light intensity focusing on the dominant plant community of Siphonales, Udotea petiolata (Turra) Borgs. and Halimeda tuna reached to the lowest depth using compressed-air aqualung. Distribution of marine algae were analyzed through quantitative method.