10 results on '"Cho, Yong-Joon"'
Search Results
2. Mycobacterium potentiates protection from colorectal cancer by gut microbial alterations.
- Author
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Kim, Yu‐Mi, Choi, Jin‐Ouk, Cho, Yong‐Joon, Hong, Bong‐Ki, Shon, Hoh‐Jeong, Kim, Bum‐Joon, Park, Joo‐Hong, Kim, Wan‐Uk, and Kim, Donghyun
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COLORECTAL cancer ,MYCOBACTERIUM bovis ,FECAL microbiota transplantation ,MYCOBACTERIUM ,MYCOBACTERIUM avium paratuberculosis ,T helper cells - Abstract
Not only are many Mycobacteria pathogens, but they can act as strong non‐specific immunopotentiators, generating beneficial effects on the pathogenesis of some diseases. However, there has been no direct evidence of the effect of mycobacterial species on colorectal cancer (CRC). Herein, we showed that there may be a meaningful inverse correlation between the incidence of tuberculosis and CRC based on global statistics and that heat‐killed Mycobacterial tuberculosis and live Mycobacterium bovis (Bacillus Calmette–Guérin strain) could ameliorate CRC development. In particular, using a faecal microbiota transplantation and a comparison between separate housing and cohousing, we demonstrated that the gut microbiota is involved in the protective effects. The microbial alterations can be elucidated by the modulation of antimicrobial activities including those of the Reg3 family genes. Furthermore, interleukin‐22 production by T helper cells contributed to the anti‐inflammatory activity of Mycobacteria. Our results revealed a novel role of Mycobacteria involving gut microbial alterations in dampening inflammation‐associated CRC and an immunological mechanism underlying the interaction between microbes and host immunity. [ABSTRACT FROM AUTHOR]
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- 2023
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3. A large‐scale metagenomic study for enzyme profiles using the focused identification of the NGS‐based definitive enzyme research (FINDER) strategy.
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Sung, Jae‐Yoon, Lee, Yong‐Jik, Cho, Yong‐Joon, Shin, Myeong‐Na, Lee, Sang‐Jae, Lee, Han‐Seung, Koh, Hong, Bae, Jin‐Woo, Shin, Jae‐Ho, Kim, Hyun Jung, and Lee, Dong‐Woo
- Abstract
Excavating the molecular details of many diverse enzymes from metagenomes remains challenging in agriculture, food, health, and environmental fields. We present a versatile method that accelerates metabolic enzyme discovery for highly selective gene capture in metagenomes using next‐generation sequencing. Culture‐independent enzyme mining of environmental DNA is based on a set of short identifying degenerate sequences specific for a wide range of enzyme superfamilies, followed by multiplexed DNA barcode sequencing. A strategy of 'focused identification of next‐generation sequencing‐based definitive enzyme research' enabled us to generate targeted enzyme datasets from metagenomes, resulting in minimal hands‐on obtention of high‐throughput biological diversity and potential function profiles, without being time‐consuming. This method also provided a targeted inventory of predicted proteins and molecular features of metabolic activities from several metagenomic samples. We suggest that the efficiency and sensitivity of this method will accelerate the decryption of microbial diversity and the signature of proteins and their metabolism from environmental samples. [ABSTRACT FROM AUTHOR]
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- 2021
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4. Functional dissection of the phosphotransferase system provides insight into the prevalence of Faecalibacterium prausnitzii in the host intestinal environment.
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Kang, Deborah, Ham, Hyeong‐In, Lee, Seung‐Hwan, Cho, Yong‐Joon, Kim, Yeon‐Ran, Yoon, Chang‐Kyu, and Seok, Yeong‐Jae
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INTESTINES ,MULTIENZYME complexes ,HUMAN microbiota ,FRUCTOSE ,CARBOHYDRATES ,DISSECTION ,PHOSPHOTRANSFERASES - Abstract
Summary: Faecalibacterium prausnitzii is a dominant member of healthy human colon microbiota, regarded as a beneficial gut bacterium due to its ability to produce anti‐inflammatory substances. However, little is known about how F. prausnitzii utilizes the nutrients present in the human gut, influencing its prevalence in the host intestinal environment. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) is a widely distributed and highly efficient carbohydrate transport system found in most bacterial species that catalyses the simultaneous phosphorylation and import of cognate carbohydrates; its components play physiological roles through interaction with other regulatory proteins. Here, we performed a systematic analysis of the 16 genes encoding putative PTS components (2 enzyme I, 2 HPr, and 12 enzyme II components) in F. prausnitzii A2‐165. We identified the general PTS components responsible for the PEP‐dependent phosphotransfer reaction and the sugar‐specific PTS components involved in the transport of two carbohydrates, N‐acetylglucosamine and fructose, among five enzyme II complexes. We suggest that the dissection of the functional PTS in F. prausnitzii may help to understand how this species outcompetes other bacterial species in the human intestine. [ABSTRACT FROM AUTHOR]
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- 2021
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5. The role of Rsv1 in the transcriptional regulation of genes involved in sugar metabolism for long‐term survival.
- Author
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Kim, Eun‐Jung, Cho, Yong‐Joon, Chung, Woo‐Hyun, and Roe, Jung‐Hye
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CYCLIC-AMP-dependent protein kinase , *PENTOSE phosphate pathway , *GENETIC regulation , *MITOGEN-activated protein kinases , *RNA sequencing , *DEOXYRIBOZYMES - Abstract
Glucose limitation is a major stress condition that cells must respond to by altering their metabolism to ensure survival. Rsv1 is a zinc finger protein previously shown to be required for survival during stationary phase. In this study, we present a novel mechanism regulated by Rsv1 in the fission yeast Schizosaccharomyces pombe that is involved in altering glucose metabolic flux. We found that rsv1 gene expression is induced by Rst2 and Atf1, two transcription factors regulated by the cAMP‐dependent protein kinase (PKA) pathway and the mitogen‐activated protein kinase (MAPK) cascade, respectively. The downstream target genes of Rsv1 were identified by genome‐wide ChIP sequencing of Rsv1‐bound DNA sites and RNA sequencing analysis of Rsv1‐dependent transcripts that were differentially expressed under glucose starvation. Rsv1 directly regulated the expression of at least 21 genes that mostly encode transporters and proteins related to sugar metabolism. Among these, gcd1, which encodes glucose dehydrogenase in the gluconate shunt for the pentose phosphate pathway, was most remarkably repressed by Rsv1. The defect in survival of Δrsv1 mutant under glucose starvation condition was mitigated by additional deletion of a gcd1, idn1, or a gene for a putative lactonase (SPCC16c4.10), suggesting the critical importance of downregulating the gluconate shunt and pentose phosphate pathway for long‐term survival. These results show an intricate response to glucose starvation: increasing the synthesis of a transcription factor via two signal transduction pathways, which sheds light on the importance of remodeling a metabolic circuit to secure glucose for cell survival. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Methane production in the oxygenated water column of a perennially ice‐covered Antarctic lake.
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Li, Wei, Dore, John E., Steigmeyer, August J., Cho, Yong‐Joon, Kim, Ok‐Sun, Liu, Yongqin, Morgan‐Kiss, Rachael M., Skidmore, Mark L., and Priscu, John C.
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METHANE ,ATMOSPHERIC methane ,GENE libraries ,CYANOBACTERIAL toxins ,GENE clusters ,LAKES ,METAGENOMICS ,PHARMACOGENOMICS - Abstract
Aerobic methane production in aquatic ecosystems impacts the global atmospheric budget of methane, but the extent, mechanism, and taxa responsible for producing this greenhouse gas are not fully understood. Lake Bonney (LB), a perennially ice‐covered Antarctic lake, has cold hypersaline waters underlying an oxygenated freshwater layer. We present temporal methane concentration profiles in LB indicating methane production in the oxygenated (> 200% air saturation) water. Experiments amended with methylphosphonate (MPn) yielded methane generation, suggesting in situ methanogenesis via the carbon‐phosphorus (C‐P) lyase pathway. Enrichment cultures from the lake were used to isolate five bacterial strains capable of generating methane when supplied with MPn as the sole P source. Based on 16S rRNA gene sequencing, the isolates belong to the Proteobacteria (closely related to Marinomonas, Hoeflea, and Marinobacter genera) and Bacteroidetes (Algoriphagus genus). 16S rRNA metagenomic sequencing confirms the presence of these taxa in LB. None of the isolated species were reported to be capable to produce methane. In addition, orthologs of the phosphoenolpyruvate mutase gene (PepM) and methylphosphonate synthase (MPnS), enzymes involved in phosphonate and MPn biosynthesis, were widely spread in the LB shotgun metagenomic libraries; genes related to C‐P lyase pathways (phn gene clusters) were also abundant. 16S rRNA and mcrA genes of anaerobic methanogens were absent in both 16S rRNA and metagenomics libraries. These data reveal that in situ aerobic biological methane production is likely a significant source of methane in LB. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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7. Whole genome sequencing analysis of the cutaneous pathogenic yeast Malassezia restricta and identification of the major lipase expressed on the scalp of patients with dandruff.
- Author
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Park, Minji, Cho, Yong‐Joon, Lee, Yang Won, and Jung, Won Hee
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NUCLEOTIDE sequencing , *SKIN inflammation , *DANDRUFF , *CRYPTOCOCCACEAE , *MALASSEZIA - Abstract
Malassezia species are opportunistic pathogenic fungi that are frequently associated with seborrhoeic dermatitis, including dandruff. Most Malassezia species are lipid dependent, a property that is compensated by breaking down host sebum into fatty acids by lipases. In this study, we aimed to sequence and analyse the whole genome of Malassezia restricta KCTC 27527, a clinical isolate from a Korean patient with severe dandruff, to search for lipase orthologues and identify the lipase that is the most frequently expressed on the scalp of patients with dandruff. The genome of M. restricta KCTC 27527 was sequenced using the Illumina MiSeq and PacBio platforms. Lipase orthologues were identified by comparison with known lipase genes in the genomes of Malassezia globosa and Malassezia sympodialis. The expression of the identified lipase genes was directly evaluated in swab samples from the scalps of 56 patients with dandruff. We found that, among the identified lipase-encoding genes, the gene encoding lipase homolog MRES_03670, named LIP5 in this study, was the most frequently expressed lipase in the swab samples. Our study provides an overview of the genome of a clinical isolate of M. restricta and fundamental information for elucidating the role of lipases during fungus-host interaction. [ABSTRACT FROM AUTHOR]
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- 2017
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8. Toll-like receptor 9 mediates oral bacteria-induced IL-8 expression in gingival epithelial cells.
- Author
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Kim, Youngsook, Jo, Ah-ram, Jang, Da Hyun, Cho, Yong-Joon, Chun, Jongsik, Min, Byung-Moo, and Choi, Youngnim
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FUNGUS-bacterium relationships ,EPITHELIAL cells ,ENZYME-linked immunosorbent assay ,B cells ,PROKARYOTES - Abstract
Previously, we reported that various oral bacteria regulate interleukin (IL)-8 production differently in gingival epithelial cells. The aim of this study was to characterize the pattern recognition receptor(s) that mediate bacteria-induced IL-8 expression. Among ligands that mimic bacterial components, only a Toll-like receptor (TLR) 9 ligand enhanced IL-8 expression as determined by ELISA. Both normal and immortalized human gingival epithelial (HOK-16B) cells expressed TLR9 intracellularly and showed enhanced IL-8 expression in response to CpG-oligonucleotide. The ability of eight strains of four oral bacterial species to induce IL-8 expression in HOK-16B cells, and their invasion capacity were examined in the absence or presence of 2% human serum. The ability of purified bacterial DNA (bDNA) to induce IL-8 was also examined. Six out of eight strains increased IL-8 production in the absence of serum. Usage of an endosomal acidification blocker or a TLR9 antagonist inhibited the IL-8 induction by two potent strains. In the presence of serum, many strains lost the ability to induce IL-8 and presented substantially reduced invasion capacity. The IL-8-inducing ability of bacteria in the absence or presence of serum showed a strong positive correlation with their invasion index. The IL-8-inducing ability of bacteria in the absence of human serum was also correlated with the immunostimulatory activity of its bDNA. The observed immunostimulatory activity of the bDNA could not be linked to its CpG motif content. In conclusion, oral bacteria induce IL-8 in gingival epithelial cells through TLR9 and the IL-8-inducing ability depends on the invasive capacity and immunostimulating DNA. [ABSTRACT FROM AUTHOR]
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- 2012
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9. Cover Image, Volume 118, Number 11, November 2021.
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Sung, Jae‐Yoon, Lee, Yong‐Jik, Cho, Yong‐Joon, Shin, Myeong‐Na, Lee, Sang‐Jae, Lee, Han‐Seung, Koh, Hong, Bae, Jin‐Woo, Shin, Jae‐Ho, Kim, Hyun Jung, and Lee, Dong‐Woo
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- 2021
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10. Influence of oipA Phase Variation on Virulence Phenotypes Related to Type IV Secretion System in Helicobacter pylori.
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Lai J, Angulmaduwa S, Kim MA, Kim A, Tissera K, Cho YJ, and Cha JH
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- Virulence genetics, Humans, Virulence Factors genetics, Virulence Factors metabolism, Phenotype, Antigens, Bacterial genetics, Antigens, Bacterial metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Helicobacter pylori genetics, Helicobacter pylori pathogenicity, Helicobacter pylori physiology, Type IV Secretion Systems genetics, Type IV Secretion Systems metabolism, Interleukin-8 metabolism, Interleukin-8 genetics, Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Helicobacter Infections microbiology
- Abstract
Background: oipA, an outer membrane protein of Helicobacter pylori, is linked to IL-8 induction and gastric inflammation, but its role is debated due to inconsistent findings. This study aims to explore the role of oipA phase variation in modulating the virulence traits of H. pylori, a bacterium strongly associated with the development of gastric cancer., Material and Methods: American clinical isolate AH868 strain for naturally occurring phase variations of the oipA gene, and G27 strain for in vitro-induced phase variations were used to elucidate oipA's impact on key virulence phenotypes, including cell elongation, CagA phosphorylation, and IL-8 induction., Results: Using AH868 strain, natural oipA phase variation does not affect cell elongation and IL-8 induction. Interestingly, however, in vitro-induced oipA phase variations in G27 strain uncovered that 9.4% of oipA "Off" transformants exhibit reduced cell elongation while all maintaining consistent IL-8 induction levels. Additionally, complementation of oipA "Off to On" status restores the cell elongation phenotype in 12.5% of transformants, highlighting the importance of oipA in maintaining normal cell morphology. Crucially, these variations in cell elongation are not linked to changes in bacterial adherence capabilities. Furthermore, the study shows a correlation among oipA phase variation, cell elongation, and CagA phosphorylation, suggesting that oipA influences the functionality of the Type IV secretion system. Whole-genome sequencing of selected transformants reveals genetic variations in bab paralogue, cagY gene, and other genomic regions, underscoring the complex genetic interactions that shape H. pylori's virulence., Conclusions: Our research provides new insights into the subtle yet significant role of oipA phase variation in H. pylori pathogenicity, emphasizing the need for further studies to explore the intricate molecular mechanisms involved. This understanding could pave the way for targeted therapeutic strategies to mitigate the impact of H. pylori on human health., (© 2024 The Author(s). Helicobacter published by John Wiley & Sons Ltd.)
- Published
- 2024
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