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Background: Conventional protocols utilize core needle biopsy (CNB) or fine needle aspiration (FNA) to produce cell suspension for flow cytometry (FCM) is a diagnostic challenge for lymphoid malignancies. We aim to develop an alternative CNB rinsing technique (RT) to produce cell suspension for FCM during this mini‐invasive procedure of CNB for lymphoma diagnosis. Methods: FNA and CNB specimens from the same lesion of 93 patients with suspected lymphoma were collected under the guidance of B‐ultrasound simultaneously. The fresh CNB samples were prepared to cell suspension by RT for FCM immunophenotyping analysis (Group CNB‐RT). Then, the CNB tissues after performing the RT process and the fresh FNA tissues were processed by conventional tissue cell suspension (TCS) technique to obtain the cell suspensions (Groups of CNB‐TCS & FNA‐TCS), respectively, as comparison. The diagnostic efficacies, as well as the concordances of the FCM results with reference to the morphologic diagnoses were compared in these three groups. Results: RT could yield sufficient cells for FCM immunophenotyping analysis, though a lower cell numbers compared to TCS technique. The diagnostic concordance was comparable in group CNB‐RT (91.1%) to the group CNB‐TCS (88.9%) and group FNA‐TCS (88.4%) (p = 0.819). The diagnostic sensitivity and specificity of CNB‐RT (91.1%; 100%) was not inferior to that of CNB‐TCS (88.9%; 100%) and FNA‐TCS (88.4%; 98.8%). Conclusions: This study shows the CNB‐RT presented non‐inferior diagnostic concordance and efficacy as compared to the TCS technique. CNB‐RT has the potential to produce cell suspension for FCM immunophenotyping while preserving tissue for lymphoma diagnosis and research. [ABSTRACT FROM AUTHOR]