Search

Data assimilation (DA) is integrated with machine learning in order to perform entirely data‐driven online state estimation. To achieve this, recurrent neural networks (RNNs) are implemented as pretrained surrogate models to replace key components of the DA cycle in numerical weather prediction (NWP), including the conventional numerical forecast model, the forecast error covariance matrix, and the tangent linear and adjoint models. It is shown how these RNNs can be initialized using DA methods to directly update the hidden/reservoir state with observations of the target system. The results indicate that these techniques can be applied to estimate the state of a system for the repeated initialization of short‐term forecasts, even in the absence of a traditional numerical forecast model. Further, it is demonstrated how these integrated RNN‐DA methods can scale to higher dimensions by applying domain localization and parallelization, providing a path for practical applications in NWP. Plain Language Summary: Weather forecast models derived from fundamental equations of physics continue to increase in detail and complexity. While this evolution leads to consistently improving daily weather forecasts, it also leads to associated increases in computational costs. In order to make a forecast at any given moment, these models must be initialized with our best guess of the current state of the atmosphere, which typically includes information from a limited set of observations as well as forecasts from the recent past. Modern methods for initializing these computer forecasts typically require running many copies of the model, either simultaneously or in sequence, to compare with observations over the recent past and ensure that our best guess estimate of the current state of the atmosphere agrees closely with those observations before making a new forecast. This repeated execution of the computer forecast model is often a time‐consuming and costly bottleneck in the initialization process. Here, it is shown that techniques from the fields of artificial intelligence and machine learning (AI/ML) can be used to produce simple surrogate models that provide sufficiently accurate approximations to replace the original costly model in the initialization phase. The resulting process is self‐contained, and does not require any further utilization of the original computer model when making daily forecasts. Key Points: Recurrent neural networks (RNNs) can replace conventional forecast models, producing accurate ensemble forecast statistics and linearized dynamicsData assimilation (DA) is compatible with RNNs by applying state estimation in the hidden state space using a modified observation operatorThe integrated RNN‐DA methods can be scaled to higher dimensions by applying domain localization techniques [ABSTRACT FROM AUTHOR]

Muscle damage after 30 maximal eccentric contractions of the elbow flexors (30MVEC) is reduced when the same exercise is performed by the opposite arm, and when two maximal voluntary isometric contractions at a long muscle length (2MVIC) are performed prior to 30MVEC by the same arm. This study investigated the hypothesis that 2MVIC would attenuate muscle damage after 30MVEC performed by the opposite arm. Untrained young (20‐25 years) men were placed into 1 of 4 experimental groups that performed 2MVIC at 1 (1d), 2 (2d), 4 (4d), or 7 days (7d) before 30MVEC by the opposite arm, or one control group that performed 30MVEC only (n = 13/group). Changes in indirect muscle damage markers after 30MVEC were compared among the groups by mixed‐design two‐way ANOVA. Maximal voluntary concentric contraction torque, range of motion, plasma creatine kinase activity, and muscle soreness did not change significantly after 2MVIC. Changes in these variables after 30MVEC were smaller (P < .05) for 1d (eg, peak soreness: 45 ± 21 mm) and 2d groups (46 ± 20 mm) than control group (66 ± 18 mm), without significant differences between 1d and 2d groups. No significant differences in the changes were found among 4d, 7d, and control groups, except for soreness showing smaller (P < .05) increases for 4d group (54 ± 19 mm) than 7d (62 ± 17 mm) and control groups. These results supported the hypothesis and showed that muscle damage induced by 30MVEC was reduced by 2MVIC performed 1‐2 days prior to 30MVIC by the contralateral arm. [ABSTRACT FROM AUTHOR]

Purpose: In conventional cytology, preparation of a specimen by wet fixation for Papanicolaou stain is potentially subject to dry effect or cell loss which may make cytologic interpretation difficult or even impossible. We have been routinely making an additional smear for rehydration with normal saline (rehydration method) before wet fixation to overcome the above shortcomings. Methods: We reviewed malignant pleural effusion and ascites 15 cases each in our cytology laboratory over the past 1 year. Four slides of each specimen were made. Two were air‐dried for Liu's stain (a Romanowsky stain) and the other two were wet‐fixed for Papanicolaou stain. The air‐dried smears were also served as retained cellularity control. One of the two wet‐fixed smears was processed as a control of preservation of nuclear detail whereas the other one stayed air‐dried for 10 minutes and then covered with normal saline (rehydration method) for 80 seconds before wet fixation. Results: There was minor cell loss (P = .032). The cells appeared larger with good preservation of nuclear detail (P < .0001 by two‐sided Wilcoxon rank sum test) but no red blood cells retained on the slide after rehydration. Conclusion: The rehydration method can effectively minimise cell loss, enlarge and preserve the cytological features of malignant cells with haemolysis. This method is simple, practical and good for cytological screening for tumour cells and interpretation especially in a bloody smear. We recommend that the rehydration method be part of traditional cytopreparatory work of wet fixation for Papanicolaou stain in conventional body fluid cytology. [ABSTRACT FROM AUTHOR]

In this Letter, the authors propose a simple carrier frequency offset estimation for universal filtered multicarrier (UFMC) systems, which not only greatly reduces the computational complexity but also promises excellent performance. First, based on the concept of the bisection method, a low-complexity selection criterion is designed to iteratively narrow the frequency range of interest. Then, the carrier frequency offset is effectively estimated using a closed-form expression derived from the Taylor series expansion technique. Computer simulations indicate that the proposed scheme can achieve performance comparable to the least squares-based estimator using high-complexity exhaustive search. [ABSTRACT FROM AUTHOR]

Barium Strontium Ferrate(BSF) is an excellent cathode material for IT-SOFCs. It is difficult to co-fire with ceria-based electrolyte due to the thermal expansion coefficient (TEC) mismatching for both materials. The ceria was mixed with BSF to be a composite cathode. The mixed composite cathode of 70 wt% BSF-30 wt% CeO2, termed as 70BSF, did not form BaCeO3 phase until 1200°C sintering temperature. The ceria could play the roles of electrocatalyst and sintering densification inhibitor in composite cathode. The BSF-containing nitrate solution was drop-coated on sintered ceria-based electrolyte to improve the adhesion of 70BSF on it. The dispersive and discontinuous BSF layer was obtained after 6 times of drop-coating. It behaved a good buffer layer to adhere the 70BSF to the ceria-based electrolyte. The AC impedance showed that the diffusion impedance reduced largely for 70BSF-ceria half-cell compared to that without BSF buffer layer. Thus, the drop-coating buffer layer of BSF solved the TEC mismatching issue. The composite cathode provided porosity and extended triple phase boundaries effectively. The half-cell performance was improved due to the interface and diffusion polarization reduction. The power density of the obtained half-cell was achieved to 495mW/cm² at 800°C. [ABSTRACT FROM AUTHOR]

The article discusses the study that determined the utility of ultrasound-guided core biopsy (USCB) for the diagnosis of base of tongue (BOT) cancer in 10 patients, finding that USCB enabled real-time evaluation and harvesting of BOT cancer. Topics discussed include usefulness of USCB for BOT cancer cases that present with normal mucosal appearance and soft tissue extension, approach to lessen possibility of tracheostomy, and utility in BOT cancer harvesting in post-irradiated patients.

Background: Pulsed radiofrequency (PRF) has been widely used to treat chronic pain, but the effectiveness and mechanisms in preventing early neuropathic pain have not been well explored. Even fewer knowledge is available in its impact on glia-mediated nociceptive sensitization. This study aims to elucidate the modulation of PRF on nerve injury-induced pain development and activation of spinal mitogen-activated protein kinases (MAPKs). Methods: In a rat spinal nerve ligation (SNL) model, a low-volt PRF treatment was applied to the L5 dorsal root ganglion after nerve injury. Nociceptive behaviours were measured by von Frey and heat withdrawal tests at multiple time points. MAPK activations, including p-ERK and p-p38, as well as TNF-á level in the spinal dorsal horn were assessed and the cell types that expressed MAPK activation were identified by double immuno fluorescence staining.Results: We found that SNL promptly induced neuropathic pain in the affected hind limb for over 1 week as well as increased p-ERK and p-p38 in the spinal dorsal horn. PRF significantly attenuated SNL-induced mechanical allodynia and thermal hyperalgesia for 5–7 days. PRF also inhibited ERK and p38 activations, which were found majorly located within neurons and microglia, respectively. Besides, PRF significantly suppressed expression of TNF-á in the spinal dorsal horn throughout the course. Conclusions: Low-volt PRF significantly ameliorated SNL-induced acute pain. Inferentially, PRF may inhibit spinal sensitization by down-regulating spinal MAPK activations and activation-mediated cytokine release.We demonstrated that early PRF treatment in acute nerve injury helps to ameliorate neuropathic pain development. [ABSTRACT FROM AUTHOR]

The article discusses a study on hypopharyngeal cancer's sampling or diagnosis with the ultrasound-guided core biopsy. Topics discussed include inclusion criteria followed to enroll patients in the study, technique used for the biopsy, and diagnosis of squamous cell carcinoma following the ultrasound-guided core biopsy in patients enrolled in the study. Other topic discussed includes approval for the study by the National Taiwan University Hospital Research Ethics Committee.

Background and Purpose: Fibroblast-to-myofibroblast differentiation is associated with scarring, an important issue in corneal surgery. Moxifloxacin (MOX), commonly applied to prevent post-operative infection, would benefit more if it modifies fibroblast-to-myofibroblast differentiation other than antimicrobial activity. Our purpose was to explore whether MOX has anti-fibrotic effect in human corneal fibroblasts (HCFs).Experimental Approach: HCFs were incubated in MOX-containing medium concurrently with TGF-β1 (co-treatment), before (pretreatment) or after (post-treatment) adding TGF-β1. HCF contractility was evaluated with a type I collagen gel contraction assay. Expression of α-smooth muscle actin (α-SMA), Smad2, phospho-Smad2-Ser467, Smad4 and Smad7 was determined by immunoblotting. Formation of α-SMA-positive filaments and distribution of active Smad2 were observed under confocal microscopy. Expression of TGF-β receptor types I (TGFBR1) and II (TGFBR2) was assessed with flow cytometry.Key Results: MOX did not affect gel contractility or α-SMA filament formation in HCFs without TGF-β1 stimulation. MOX did, however, retard HCF-containing gel contractility and α-SMA filament formation following TGF-β1 stimulation in the pretreatment and co-treatment groups but not in the post-treatment group. MOX blocked the expression of Smad2, phospho-Smad2-Ser467 and TGFBR1 under TGF-β1 incubation. Additionally, MOX enhanced Smad7 expression in TGF-β1-incubated HCFs, but did not interfere with TGF-β-triggered Smad2 nuclear translocation or Smad4 expression.Conclusions and Implications: MOX inhibited TGF-β-induced fibroblast-to-myofibroblast differentiation via blocking TGFBR1 and enhancing Smad7 expression. MOX should be used before or during surgery to achieve these effects. These results suggest a de novo mechanism by which MOX participates in corneal wound healing. [ABSTRACT FROM AUTHOR]

Calla lily chlorotic spot virus (CCSV) collected from calla lily plants showing symptoms in Taiwan has been identified as a tentative species of the genus Tospovirus based on the comparison of S RNA sequences. In this investigation, the complete sequences of its L and M RNAs were determined. The L RNA contains 8911 nucleotides (nt) and encodes a putative RNA-dependent RNA polymerase of 2882 amino acids (aa) (332 kDa) in the viral complementary (vc) sense. The M RNA was shown to be 4704 nt in length, encoding a nonstructural NSm protein of 309 aa (35 kDa) in the viral sense and a Gn/Gc glycoprotein precursor (GP) of 1123 aa (128 kDa) in the vc sense. Phylogenetic analysis of the individual tospoviral proteins indicated that CCSV is closely related to Tomato zonate spot virus, a new tentative tospovirus isolated from tomato in southern China. Two degenerate primer pairs gL2740/gL3920c and gM410/gM870c, designed, after sequence comparison, from the highly conserved regions of the L and NSm genes, respectively, were successfully used to detect 12 greenhouse-cultured tospoviruses, including six formal species and six tentative species, by reverse transcription-polymerase chain reaction. They were also used to detect Melon yellow spot virus and Watermelon silver mottle virus from field cucurbit samples in Taiwan. The results indicate that the two degenerate primer pairs can be used for prompt detection of most tospoviruses and exploration of unknown tospovirus species. [ABSTRACT FROM AUTHOR]

Summary. Hepatitis B virus (HBV) genotypes/mutants are known to affect natural outcomes. The virologic differences among HBV genotype, precore and basal core promoter (BCP) mutations were investigated. HBV strains were isolated from 18 hepatitis B e antigen (HBeAg)-positive patients (nine genotype B and nine genotype C). All had precore and BCP wild-type sequences. After cloning of full-length HBV genome, the effects of viral genotype, precore and BCP mutations singly or additively on the expression of viral DNA and antigens were investigated by mutagenesis and transfection assays in Huh7 cells. Significant findings included the following: (i) expression of intracellular core protein increased when precore or BCP mutation was introduced in genotype C strains; (ii) expression of intracellular surface protein was lower in genotype C precore wild-type strain compared with genotype B; (iii) precore mutation was associated with a lower extracellular expression level of HBV DNA; (iv) secretion of hepatitis B surface antigen in genotype C was lower than that in genotype B; and (v) secretion of HBeAg in genotype B was lower than that in genotype C. No additive effect was observed by combining precore and BCP mutations. Hence, HBV genotype and precore/BCP mutations correlate with intrahepatic expression of viral antigens in vitro. [ABSTRACT FROM AUTHOR]

A look-back study was conducted to determine the clinical significance of occult hepatitis B virus (HBV) blood transfusion in an HBV hyperendemic area. To improve the blood transfusion safety. Occult HBV is transmissible through blood transfusion in HBV-naÏve recipients. However, its impact on recipients with prevalent HBV infection in HBV hyperendemic areas is unclear. In 2006, 12 occult HBV blood donors were found from 10 824 repository samples by nucleic acid testing. The 74 corresponding recipients were identified and their pre- and post-transfusion clinical information was gathered, and the living recipients were recalled for follow-up. From the available archival sera, the HBV DNA was examined and sub-genomic sequences between paired donor and recipient were compared using polymerase chain reaction-based assays. Among the 74 recipients, 18 were still alive and 12 returned to our clinic. From the available serological profiles, 76% of recipients had ongoing or recovered HBV infection before transfusion. Only 24 recipients had available post-transfusion serological profiles and none seroconverted to be hepatitis B surface antigen (HBsAg) positive. Moreover, except for the prior HBsAg carriers, the recipients' HBV DNA levels after transfusion were low (<20 IU/mL). One recipient had identical HBV surface gene sub-genomic sequence (384 nucleotides) to his donor. After transfusion, no recipient developed post-transfusion hepatitis (PTH) and the clinical outcome was good. In HBV hyperendemic areas, occult hepatitis B transfusion might not lead to HBsAg carriage or PTH. The risk of transfusion-transmitted HBV infection was probably lower than that in non-endemic areas because most recipients had already experienced HBV infection. [ABSTRACT FROM AUTHOR]

Ghosh A, Joo NE, Chen TC, Kapila YL. Proapoptotic fibronectin fragment induces the degradation of ubiquitinated p53 via proteasomes in periodontal ligament cells. J Periodont Res 2010; 45: 481–487. © 2010 John Wiley & Sons A/S Background and Objective: The extracellular matrix (ECM) plays a key role in signaling necessary for tissue remodeling and cell survival. However, signals from the ECM altered by disease, e.g. inflammatory diseases such as periodontitis and arthritis, may lead to apoptosis or programmed cell death of resident cells. Previously, we found that a disease-associated fibronectin fragment triggers apoptosis of primary human periodontal ligament cells via a novel apoptotic pathway in which the tumor suppressor, p53, is transcriptionally downregulated. Material and Methods: We used immunofluorescence, transfection assays, western blotting and ELISAs to show that p53 is degraded by a proteasomal pathway in response to a proapoptotic disease-associated fibronectin fragment. Results: We found that in these apoptotic conditions, p53 is further downregulated by post-translational ubiquitination and subsequent targeting to proteasomes for degradation. Pretreatment of cells with the proteasomal inhibitors MG132 and lactacystin rescued the cells from apoptosis. The p53 levels in cells transfected with ubiquitin small interfering RNA were resistant to degradation induced by the proapoptotic fibronectin fragment, showing that ubiquitination is important for the proapoptotic fibronectin fragment-induced degradation of p53. Conclusion: These data show that a proapoptotic fibronectin matrix induces ubiquitination and degradation of p53 in the proteasome as part of a novel mechanism of apoptosis associated with inflammatory diseases. [ABSTRACT FROM AUTHOR]

We performed a retrospective review of cases of human immunodeficiency virus-associated progressive multifocal leucoencephalopathy in four hospitals (three in Australia and one in Hong Kong) between 1987 and 2003 in order to describe the local experience with this disease and to evaluate parameters impacting upon survival. Eighty-seven cases were identified and demographic details, baseline parameters and treatment methods and response were described. Survival was substantially increased in the post-highly active antiretroviral therapy (HAART) era with a median survival increase from 14 to 64 weeks. On multivariate analysis, variables associated with prolonged survival included a CD4 count of >100 cells/ μl at diagnosis and the use of HAART post-diagnosis, with no significant additional advantage from the use of neuroactive antiretrovirals. [ABSTRACT FROM AUTHOR]

Dealing with the transmission network of electric power systems, this paper identifies the sequential failures of receiving station components under a severe earthquake. Such failures progressively degrade the power network performance, potentially leading to a network blackout. Hence, in this paper, these components are incorporated into the network systems analysis taking their seismic vulnerability into consideration in the form of fragility curves for the evaluation of the network performance under seismic conditions. Specifically, most critical to the operation of transmission network, transformers, disconnect switches, circuit breakers and buses are integrated into the systems analysis, exploring the possibility of progressive failures of these components. The scenarios of these component failures can make it possible to estimate quantitatively direct cost of damage, emergency repair and restoration, and duration of power interruption and attendant economic losses and societal disruption. Copyright © 2006 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Adefovir dipivoxil is effective against lamivudine-resistant hepatitis B virus (HBV) strains. Whether short-term overlap lamivudine is beneficial remains unknown, particularly in patients with decompensated chronic hepatitis B. We enrolled 30 patients who underwent 48-week adefovir treatment (10 mg daily) for exacerbation of hepatitis B, associated with lamivudine-resistant mutants. Nineteen (63.3%) patients had baseline evidence of hepatic decompensation. Lamivudine was combined for ≤1 month in eight (group I), 2–5 months in 10 (group II) and ≥6 months in 12 (group III). We analysed their serial alanine aninotransferase (ALT) levels, Child–Pugh (CP) score, serum viral load and lamivudine-resistant strains. We found that serum ALT became normalized in 20 (66.7%) and HBV-DNA decreased to ≤100 copies/mL in eight (26.7%) at the end of the 48-week treatment. The log(10) reduction of serum HBV-DNA was significantly smaller in group I patients compared with group II and III patients at week 24 and 48 of treatment [median (range): 3.0 (1.5–5.6) vs 4.5 (1.5–7.4), P = 0.032; and 3.4 (0.9–4.7) vs 5.2 (2.2–7.7), P = 0.008 respectively]. In contrast, the virologic responses at the end of the 48-week therapy were similar between group II and III patients. The improvement in serum ALT and CP score at week 48 was similar irrespective of baseline decompensation, liver cirrhosis and the duration of overlap lamivudine therapy. Our findings suggested that an overlap of lamivudine for ≥2 months might lead to better virological but not biochemical outcomes in patients receiving adefovir for lamivudine-resistance HBV. As our sample size was small and the study was not randomly controlled, further studies are needed. [ABSTRACT FROM AUTHOR]

Channel catfish (Ictalurus punctatus) fillets were treated with hydrogen peroxide (HP), ozonated water (OZ), and ascorbic acid plus salt (AS) or salt solution (BR). All treatments except AS decreased (p ≥ 0.05) Aeromonas, while OZ did not decrease Staphylococcus and Acinetobacter. All treatments except 5 ppm OZ reduced {p ≥ 0.05) initial TCC and PPC. At 0.7% HP, BR, and 10 ppm OZ fillets showed odor spoilage after 8, 10 and 10.5 d, respectively. Fillets treated with HP, OZ, and BR bad higher (p ≥ 0.05) TBARs than control fillets. HP treated fillets had lower (p ≥ 0.05) Hunter 'L' values, while BR treated fillets had higher 'a' and lower (p ≥ 0.05) Hunter 'L', 'b', and SI values than controls. [ABSTRACT FROM AUTHOR]

Increasing evidence suggests involvement of integrins and CD44 isoforms in the pathogenesis of psoriasis, contributing to uncontrolled keratinocyte proliferation, neovascularization, and invasion of inflammatory cells. We have analyzed immunohistochemically in situ expression of integrins (CD29, CDw49b, CDw49c, CDw49e, CDw49f) and CD44 isoforms (CD44 standard, CD44 var/v6, CD44 v10) on frozen sections of normal and psoriatic skin (nonlesional skin, lesional skin before and along with topical calcitriol treatment). We did not observe visual changes of immunoreactivity in normal as compared to nonlesional psoriatic skin, while the staining pattern of CDw49c, CDw49f, and CD29 was severely altered in untreated lesional psoriatic skin. Most markedly, CDw49c, CDw49f, and CD29 were focally upregulated in suprapapillar epidermal compartments of lesional psoriatic skin, a staining pattern that is in accordance with the phenomenon that was described by Pinkus as "squirting papilla". Additionally, an increased proportion of inflammatory and endothelial cells revealed immunoreactivity for CD44(std.) in untreated lesional psoriatic as compared to nonlesional psoriatic or normal skin. After 8 weeks of topical calcitriol treatment (15 μg/g ointment), the staining pattern for CDw49c, CDw49f and CD29 was markedly changed in epidermis of lesional psoriatic skin, reverting to the staining pattern characteristic for the nonlesional psoriatic or normal human skin, although epidermal expression of CDw49f was still upregulated and CDw49e-, CDw49f-, CD29-, and CD44(std.)- immunoreactive inflammatory and endothelial cells were still to be found in the dermal compartment. [ABSTRACT FROM AUTHOR]

BSTRACT. Theoretical and practical results are summarized for a study to determine optimal water resource allocation in a proposed water conservancy district. The area of this district, which covers several river basins, contains a large number of existing and proposed facilities such as reservoirs and diversions. The operation of all of these facilities was to be determined along with the sizing of the proposed facilities in order to optimize given objective functions. Related efforts in optimal river basin utilization were surveyed, and linear programming was selected as an expedient optimization technique. The problem is formulated by identifying time stages which together constitute a repetitive cycle such as a year. With these stages, it is possible to associate operational and capacity variables with network components, which are branches and nodes. Objective functions are assembled for the component variables. Constraint equations are written in terms of the variables to reflect network nodal continuity, capacity restrictions, and adjudications such as water rights. A numerical example is considered in which the existing and proposed facilities are aggregated to produce a small, tractable number of facilities. This paper examines the example results and suggests future improvements for models of this type. [ABSTRACT FROM AUTHOR]

Intersphincteric resection (ISR) was first described in the 1980s as a surgical technique for anal preservation in patients with rectal cancer within the anal canal, and preliminary results suggested acceptable oncological outcomes when compared with abdominoperineal excision [[1]]. ISR generally involves mobilization of the colorectum abdominally, then a perineal phase for resection of the internal anal sphincter ensuring an adequate tumour margin, followed by a hand-sewn coloanal anastomosis. 3 Braun J, Treutner KH, Winkeltau G, Heidenreich U, Lerch MM, Schumpelick V. Results of intersphincteric resection of the rectum with direct coloanal anastomosis for rectal carcinoma. [Extracted from the article]

Kamaboko-like products, prepared with NaCl and phosphate buffer wash solutions, had higher yields compared to those prepared with Na-acetate and polyphosphate solutions. Products prepared with polyphosphate wash solution were lightest in color. Higher fat in mechanically deboned poultry meat caused lower yields than lower fat. There were no interactions (P>0.05) between the temperatures and salt concentrations of the wash solutions on yields of products washed with various concentrations of NaCl. Products prepared with NaCl wash solutions at 2-4°C had higher yields than those prepared at 20-22°C. Generally, products prepared with 1.0% or 1.5% NaCl wash solutions had higher Hunter "L" values and lower Hunter "a" values when compared to those prepared with 0 or 0.5% NaCl washing solutions. [ABSTRACT FROM AUTHOR]

The article presents the first record of Melon yellow spot virus (MYSV) in watermelon in Taiwan. A survey on thrips-borne tospovirus called Watermelon silver mottle virus (WSMoV) on watermelon (Citrullus lanatus)was conducted in June 2006 using the antiserum against the nucleocapsid (N) protein of Capsicum chlorosis virus. Extracts from diseased samples with a positive reaction were used as inocula for bioassay on watermelon and Chenopodium quinoa, where one has shown symptoms different from those caused by WSMoV. The reaction of the new isolate with the monoclonal antibody (MAb) against the nonstructural WSMoV NSs protein suggested that tospovirus was only distantly related to WSMoV. The result indicated that the new virus is an isolate of MYSV, a virus previously reported in Japan.