9 results on '"Blenkiron, Cherie"'
Search Results
2. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.
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Welsh, Joshua A., Goberdhan, Deborah C. I., O'Driscoll, Lorraine, Buzas, Edit I., Blenkiron, Cherie, Bussolati, Benedetta, Cai, Houjian, Di Vizio, Dolores, Driedonks, Tom A. P., Erdbrügger, Uta, Falcon‐Perez, Juan M., Fu, Qing‐Ling, Hill, Andrew F., Lenassi, Metka, Lim, Sai Kiang, Mahoney, Mỹ G., Mohanty, Sujata, Möller, Andreas, Nieuwland, Rienk, and Ochiya, Takahiro
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EXTRACELLULAR vesicles ,CELL culture ,SCIENTIFIC discoveries ,TASK forces ,RESEARCH personnel ,BODY fluids - Abstract
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly. [ABSTRACT FROM AUTHOR]
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- 2024
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3. A Novel Electrochemically Switchable Conductive Polymer Interface for Controlled Capture and Release of Chemical and Biological Entities.
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Akbarinejad, Alireza, Hisey, Colin Lee, Martinez-Calderón, Miguel, Low, Jeffery, Bryant, Devon T., Zhu, Bicheng, Brewster, Diane, Chan, Eddie Wai Chi, Ashraf, Jesna, Wan, Ziyao, Artuyants, Anastasiia, Blenkiron, Cherie, Chamley, Larry, Barker, David, Williams, David E., Evans, Clive W., Pilkington, Lisa I., and Travas-Sejdic, Jadranka
- Abstract
Materials platforms that enable controlled isolation and subsequent release of chemical/biological entities are in great demand for a diverse range of practical applications. Current technologies lack good control and efficiency of the release, which is needed to preserve the captured targets of interest. Here, this need is addressed by providing a versatile, controllable, electrochemical capture/release interface. The interface consists of a highly porous electrospun membrane, electrodeposited with a thiol-functionalized 3,4-ethyl-enedioxythiophene (EDOT) conductive terpolymer, in which the thiol moiety undergoes oxidation/reduction cycles at moderate potentials (+1.0 and -0.8 V, respectively) to enable capture/release. The fast oxidative capture (1 min) and reductive release (2 min) of a model thiol molecule in a highly controllable manner, followed by successful capture/release of an antibody, are demon- strated. Then, femtosecond laser-patterning is used to fabricate an array of ≈30 µm pores on the electrospun membrane, subsequently coated with the conducting terpolymer, enabling the highly efficient (>90%), fast (20 min) and selective capture of MCF7 cancer cells with 33% release efficiency when polarized at -0.8 V. The released cells show a high level of viability, indicating the capture and release process does not affect cell survival. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles.
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Witwer, Kenneth W, Goberdhan, Deborah CI, O'Driscoll, Lorraine, Théry, Clotilde, Welsh, Joshua A, Blenkiron, Cherie, Buzás, Edit I, Di Vizio, Dolores, Erdbrügger, Uta, Falcón‐Pérez, Juan M, Fu, Qing‐Ling, Hill, Andrew F, Lenassi, Metka, Lötvall, Jan, Nieuwland, Rienk, Ochiya, Takahiro, Rome, Sophie, Sahoo, Susmita, and Zheng, Lei
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EXTRACELLULAR vesicles ,BOARDS of directors - Abstract
The minimal information for studies of extracellular vesicles (EVs, MISEV) is a field‐consensus rigour initiative of the International Society for Extracellular Vesicles (ISEV). The last update to MISEV, MISEV2018, was informed by input from more than 400 scientists and made recommendations in the six broad topics of EV nomenclature, sample collection and pre‐processing, EV separation and concentration, characterization, functional studies, and reporting requirements/exceptions. To gather opinions on MISEV and ideas for new updates, the ISEV Board of Directors canvassed previous MISEV authors and society members. Here, we share conclusions that are relevant to the ongoing evolution of the MISEV initiative and other ISEV rigour and standardization efforts. [ABSTRACT FROM AUTHOR]
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- 2021
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5. Biodistribution of extracellular vesicles following administration into animals: A systematic review.
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Kang, Matthew, Jordan, Vanessa, Blenkiron, Cherie, and Chamley, Lawrence W.
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EXTRACELLULAR vesicles ,SPLEEN ,LUNGS ,LIVER - Abstract
In recent years, attention has turned to examining the biodistribution of EVs in recipient animals to bridge between knowledge of EV function in vitro and in vivo. We undertook a systematic review of the literature to summarize the biodistribution of EVs following administration into animals. There were time‐dependent changes in the biodistribution of small‐EVs which were most abundant in the liver. Detection peaked in the liver and kidney in the first hour after administration, while distribution to the lungs and spleen peaked between 2–12 h. Large‐EVs were most abundant in the lungs with localization peaking in the first hour following administration and decreased between 2–12 h. In contrast, large‐EV localization to the liver increased as the levels in the lungs decreased. There was moderate to low localization of large‐EVs to the kidneys while localization to the spleen was typically low. Regardless of the origin or size of the EVs or the recipient species into which the EVs were administered, the biodistribution of the EVs was largely to the liver, lungs, kidneys, and spleen. There was extreme variability in the methodology between studies and we recommend that guidelines should be developed to promote standardization where possible of future EV biodistribution studies. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Short‐term high‐intensity interval training exercise does not affect gut bacterial community diversity or composition of lean and overweight men.
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Rettedal, Elizabeth A., Cree, Julia M. E., Adams, Shannon E., MacRae, Caitlin, Skidmore, Paula M. L., Cameron‐Smith, David, Gant, Nicholas, Blenkiron, Cherie, and Merry, Troy L.
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HIGH-intensity interval training ,BACTERIAL diversity ,CARDIOVASCULAR fitness ,BACTERIAL communities ,OVERWEIGHT men ,AEROBIC bacteria ,BACTERIOPLANKTON - Abstract
New Findings: What is the central question of this study?Does short‐term high‐intensity interval training alter the composition of the microbiome and is this associated with exercise‐induced improvements in cardiorespiratory fitness and insulin sensitivity?What is the main finding and its importance?Although high‐intensity interval training increased insulin sensitivity and cardiovascular fitness, it did not alter the composition of the microbiome. This suggests that changes in the composition of the microbiome that occur with prolonged exercise training might be in response to changes in metabolic health rather than driving exercise training‐induced adaptations. Regular exercise reduces the risk of metabolic diseases, and the composition of the gut microbiome has been associated with metabolic function. We investigated whether short‐term high‐intensity interval training (HIIT) altered the diversity and composition of the bacterial community and whether there were associations with markers of insulin sensitivity or aerobic fitness. Cardiorespiratory fitness (V̇O2peak) and body composition (dual energy X‐ray absorptiometry scan) were assessed and faecal and fasted blood samples collected from 14 lean (fat mass 21 ± 2%, aged 29 ± 2 years) and 15 overweight (fat mass 33 ± 2%, aged 31 ± 2 years) men before and after 3 weeks of HIIT training (8–12 × 60 s cycle ergometer bouts at V̇O2peak power output interspersed by 75 s rest, three times per week). Gut microbiome composition was analysed by 16S rRNA gene amplicon sequencing. The HIIT significantly increased the aerobic fitness of both groups (P < 0.001) and improved markers of insulin sensitivity (lowered fasted insulin and HOMA‐IR; P < 0.001) in the overweight group. Despite differences in the abundance of several bacterial taxa being evident between the lean and overweight group, HIIT did not affect the overall bacterial diversity or community structure (α‐diversity or β‐diversity). No associations were found between the top 50 most abundant bacterial genera and cardiorespiratory fitness markers; however, significant associations (P < 0.05) were observed between the abundance of the bacterial species Coprococcus_3, Blautia, Lachnospiraceae_ge and Dorea and insulin sensitivity markers in the overweight group. Our results suggest that short‐term HIIT does not greatly impact the overall composition of the gut microbiome, but that certain microbiome genera are associated with insulin sensitivity markers that were improved by HIIT in overweight participants. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions.
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Hong, Jiwon, Dauros-Singorenko, Priscila, Whitcombe, Alana, Payne, Leo, Blenkiron, Cherie, Phillips, Anthony, and Swift, Simon
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DENSITY gradient centrifugation ,GEL permeation chromatography ,FALSE discovery rate ,MASS spectrometry ,BACTERIAL cultures ,MOLECULAR chaperones - Abstract
Bacteria release nano-sized extracellular vesicles (EVs) into the extracellular milieu. Bacterial EVs contain molecular cargo originating from the parent bacterium and have important roles in bacterial survival and pathogenesis. Using 8-plex iTRAQ approaches, we profiled the EV proteome of two Escherichia coli strains, uropathogenic (UPEC) 536 and probiotic Nissle 1917. For these strains, we compared the proteome of crude input EVs prepared by ultracentrifugation alone with EVs purified by either density gradient centrifugation (DGC) or size exclusion chromatography (SEC). We further compared the proteome of EVs from bacterial cultures that were grown in iron-restricted (R) and iron-supplemented (RF) conditions. Overall, outer membrane components were highly enriched, and bacterial inner membrane components were significantly depleted in both UPEC and Nissle EVs, in keeping with an outer membrane origin. In addition, we found enrichment of ribosome-related Gene Ontology terms in UPEC EVs and proteins involved in glycolytic processes and ligase activity in Nissle EVs. We have identified that three proteins (RbsB of UPEC in R; YoeA of UPEC in RF; BamA of Nissle in R) were consistently enriched in the DGC- and SEC-purified EV samples in comparison to their crude input EV, whereas conversely the 60 kDa chaperonin GroEL was enriched in the crude input EVs for both UPEC and Nissle in R condition. Such proteins may have utility as technical markers for assessing the purity of E. coli EV preparations. Several proteins were changed in their abundance depending on the iron availability in the media. Data are available via ProteomeXchange with identifier PXD011345. In summary, we have undertaken a comprehensive characterization of the protein content of E. coli EVs and found evidence of specific EV cargos for physiological activity and conserved protein cargo that may find utility as markers in the future. Abbreviation: DGC: density gradient centrifugation; DTT: 1,4-dithiothreitol; EV: extracellular vesicles; FDR: false discovery rate; GO: Gene Ontology; R: iron-restricted; RF: iron-supplemented; iTRAQ: isobaric tags for relative and absolute quantitation; OMV: outer membrane vesicle; SWATH-MS: sequential window acquisition of all theoretical mass spectra; SEC: size exclusion chromatography. [ABSTRACT FROM AUTHOR]
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- 2019
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8. The transcriptional responses of cultured wound cells to the excretions and secretions of medicinal L ucilia sericata larvae.
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Dauros Singorenko, Priscila, Rosario, Roseanne, Windsor, John A., Phillips, Anthony R., and Blenkiron, Cherie
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RNA analysis ,CELL culture ,CELL lines ,CELL physiology ,COMPLEMENT (Immunology) ,FIBROBLASTS ,FLUORIMETRY ,GENE expression ,INSECT larvae ,INTERLEUKINS ,KERATINOCYTES ,MAGGOT therapy ,MONOCYTES ,NEOVASCULARIZATION ,POLYMERASE chain reaction ,RESEARCH funding ,SECRETION ,WOUND healing ,PHENOTYPES ,REVERSE transcriptase polymerase chain reaction ,CHRONIC wounds & injuries ,LIPOPOLYSACCHARIDES ,GENE expression profiling ,IN vitro studies ,THERAPEUTICS - Abstract
Maggots, through their excretions and secretions (ES), promote wound healing by removing necrotic tissue, counter bacterial infection, and activate wound associated cells. We investigated the effects of a physiological dose of maggot ES on four wound-associated cell types in vitro with Affymetrix gene expression arrays; keratinocytes, endothelial cells, fibroblasts, and monocytes. Keratinocytes showed the fewest ( n = 5; p < 0.05, fold-change ±2) and smallest fold-changes (up to 2.32×) in gene expression and conversely THP1 monocytes had the most ( n = 233) and greatest magnitude (up to 44.3×). There were no genes that were altered in all four cell-lines. Gene pathway analysis identified an enrichment of immune response pathways in three of the treated cell-lines. Analyses by quantitative RT-PCR found many genes dynamically expressed in ES dose dependent manner during the three day treatments. Phenotype analyses, however, found no effects of ES on cell viability, proliferation, migration and angiogenesis. ES was 100× less potent at triggering IL-8 secretion than fibroblasts treated with purified bacterial lipopolysaccharide (LPS; in equivalent amounts to that found in ES; ∼40 EU/ml). Furthermore, co-treatment with LPS and ES decreased the LPS-alone triggered IL-8 secretion by 13%. Although ES had no direct effect on wound cell phenotypes it did partially reduce the immune response to bacterial LPS exposure. These observations were consistent with the profile of transcriptional responses that were dominated by modulation of immune response genes. Maggot therapy may therefore improve wound healing through the secondary effects of these gene changes in the wound cells. [ABSTRACT FROM AUTHOR]
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- 2017
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9. Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity.
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Dauros Singorenko, Priscila, Chang, Vanessa, Whitcombe, Alana, Simonov, Denis, Hong, Jiwon, Phillips, Anthony, Swift, Simon, and Blenkiron, Cherie
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VESICLES (Cytology) ,PROKARYOTES ,HETEROGENEITY ,DENSITY gradient centrifugation ,ENDOTOXINS - Abstract
Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability and natural biological heterogeneity seen between DGC preparations of MVs forMycobacterium smegmatisandEscherichia coliand compare these DGC data with size exclusion chromatography (SEC) columns. Crude preparations of MVs, isolated from cultures by ultrafiltration and ultracentrifugation were separated by DGC with fractions manually collected as guided by visible bands. Yields of protein, RNA and endotoxin, protein banding and particle counts were analysed in these. DGC and SEC methods enabled separation of molecularly distinct MV populations from crude MVs. DGC banding profiles were unique for each of the two species of bacteria tested and further altered by changing culture conditions, for example with iron supplementation. SEC is time efficient, reproducible and cost effective method that may also allow partial LPS removal from Gram-negative bacterial MVs. In summary, both DGC and SEC are suitable for the separation of mixed populations of MVs and we advise trials of both, coupled with complete molecular and single vesicle characterisation prior to downstream experimentation. [ABSTRACT FROM PUBLISHER]
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- 2017
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