1. Developing an authentication approach using SPME-GC-IRMS based on compound-specific δ 13C analysis of six typical volatiles in wine.
- Author
-
(金新宇), Xinyu Jin, (张立敏), Limin Zhang, (吴时敏), Shimin Wu, (黄明泉), Mingquan Huang, (喻文娟), Wenjuan Yu, and (张珊珊), Shanshan Zhang
- Subjects
POLYDIMETHYLSILOXANE ,GAS chromatography/Mass spectrometry (GC-MS) ,SOLID phase extraction ,MARKET volatility ,ACCURACY - Abstract
An analytical method using gas chromatography isotope ratio mass spectrometry (GC-IRMS) combined with solid phase micro-extraction (SPME) was developed to measure the δ
13 C values of six typical volatiles commonly occurring in wine (isoamyl acetate, 2-octanone, limonene, 2-phenylethanol, ethyl octanoate and ethyl decanoate) for the first time. SPME selected with a divinylbenzene/carboxen/polydimethylsiloxane fiber was combined with the GC-IRMS for pretreatment optimization. The optimized SPME parameters of extraction time, extraction temperature and salt concentration were 40 min, 40 °C and 10%, respectively. The δ13 C values measured by SPME-GC-IRMS were in good agreement with those measured via elemental analyzer (EA)-IRMS and GC-IRMS. The differences range from 0.02 to 0.44‰ with EA-IRMS and from 0 to 0.28‰ with GC-IRMS, indicating the high accuracy of the method. This newly established method measured the precision within 0.30‰ and was successfully validated to discriminate imported real wine samples with identical label but amazing price differences from different importers. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF