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Pharmacogenomics (PGx) decision support and return of results is an active area of precision medicine. One challenge of implementing PGx is extracting genomic variants and assigning haplotypes in order to apply prescribing recommendations and information from the Clinical Pharmacogenetics Implementation Consortium (CPIC), the US Food and Drug Administration (FDA), the Pharmacogenomics Knowledgebase (PharmGKB), etc. Pharmacogenomics Clinical Annotation Tool (PharmCAT) (i) extracts variants specified in guidelines from a genetic data set derived from sequencing or genotyping technologies, (ii) infers haplotypes and diplotypes, and (iii) generates a report containing genotype/diplotype‐based annotations and guideline recommendations. We describe PharmCAT and a pilot validation project comparing results for 1000 Genomes Project sequences of Coriell samples with corresponding Genetic Testing Reference Materials Coordination Program (GeT‐RM) sample characterization. PharmCAT was highly concordant with the GeT‐RM data. PharmCAT is available in GitHub to evaluate, test, and report results back to the community. As precision medicine becomes more prevalent, our ability to consistently, accurately, and clearly define and report PGx annotations and prescribing recommendations is critical. [ABSTRACT FROM AUTHOR]

In lithium metal batteries (LMBs), the reaction between metallic Li and organic electrolytes consumes both Li and electrolyte, while the inhomogeneity of the resulting solid-electrolyte interphase (SEI) contributes to inhomogeneous Li deposition. An artificial SEI, made by coating the Li surface with an electronically insulating material, can mitigate problems caused by uncontrolled SEI formation by 1) decreasing reduction of the electrolyte on the surface, and 2) increasing the uniformity of the Li+ flux to the electrode surface, thereby mitigating dendrite growth. In this work, we present a technique for coating the surface of LMB electrodes with sulfur-rich molybdenum sulfide. This coating acts as an artificial SEI, which improves the coulombic efficiency and cycling stability of the LMBs by improving the reaction kinetics and decreasing the surface area of the exposed Li. [ABSTRACT FROM AUTHOR]

The ocean transports vast amounts of heat around the planet, helping to regulate regional climate. One important component of this heat transport is the movement of warm water from equatorial regions toward the poles, with colder water flowing in return. Here, we introduce a framework relating meridional heat transport to the diabatic processes of surface forcing and turbulent mixing that move heat across temperature classes. Applied to a (1/4)° global ocean model the framework highlights the role of the tropical Indo‐Pacific in the global ocean heat transport. A large fraction of the northward heat transport in the Atlantic is ultimately sourced from heat uptake in the eastern tropical Pacific. Turbulent mixing moves heat from the warm, shallow Indo‐Pacific circulation to the cold deeper‐reaching Atlantic circulation. Our results underscore a renewed focus on the tropical oceans and their role in global circulation pathways. Key Points: Heat budget analysis reveals links between meridional and diathermal ocean heat transportsLarge fraction of Atlantic northward heat transport is sourced from eastern tropical Pacific heat uptakeTurbulent mixing transfers heat from warm shallow Indo‐Pacific circulation to cold deep‐reaching Atlantic circulation [ABSTRACT FROM AUTHOR]

Near‐surface air quality (AQ) observations over coastal waters are scarce, a situation that limits our capacity to monitor pollution events at land‐water interfaces. Satellite measurements of total column (TC) nitrogen dioxide (NO2) observations are a useful proxy for combustion sources, but the once daily snapshots available from most sensors are insufficient for tracking the diurnal evolution and transport of pollution. Ground‐based remote sensors like the Pandora Spectrometer Instrument (PSI) that have been developed to verify space‐based TC NO2 and other trace gases are being tested for routine use as certified AQ monitors. The KORUS‐OC (Korea‐United States Ocean Color) cruise aboard the R/V Onnuri in May–June 2016 represented an opportunity to study AQ near the South Korean coast, a region affected by both local/regional and long‐distance pollution sources. Using PSI data in direct‐Sun mode and in situ sensors for shipboard ozone, CO, and NO2, we explore, for the first time, relationships between TC NO2 and surface AQ in this coastal region. Three case studies illustrate the value of the PSI and complexities in the surface‐column NO2 relationship caused by varying meteorological conditions. Case Study 1 (25–26 May 2016) exhibited a high correlation of surface NO2 to TC NO2 measured by both PSI and Aura's Ozone Monitoring Instrument, but two other cases displayed poor relationships between in situ and TC NO2 due to decoupling of pollution layers from the surface. With suitable interpretation the PSI TC NO2 measurement demonstrates good potential for working with upcoming geostationary satellites to advance diurnal tracking of pollution. Key Points: Pandora NO2 total column and coincident surface O3, NO2, and CO were measured off coastal Korea in May–June 2016Relationship between Pandora column and surface NO2 depends on meteorology with only one of three cases well correlatedAir pollution along Korean coast originates from both South Korean and regional sources [ABSTRACT FROM AUTHOR]

The aim of this study was to examine how running experience affects leg stiffness (Kleg) and spring-mass characteristics during running stages associated with the onset of blood lactate accumulation (OBLA). Seven trained (66.9 ± 4.8 kg; 182 ± 4.0 cm; 23.1 ± 3.1 years) and 13 untrained (78.5 ± 7.6 kg; 182 ± 3.0 cm; 20.3 ± 1.5 years) runners completed an incremental treadmill run. Running velocity was increased by 1 km.h−1 every four minutes and blood lactate samples were taken at every stage, in addition to a 10 s video recording using 'Runmatic'. Once 4 mmolL−1 (OBLA; the second lactate turn point) had been reached one more stage was completed. Spring-mass characteristics across groups and at pre-OBLA, OBLA and post-OBLA were compared. The velocity at OBLA was higher for the trained runners compared to the untrained runners (18 ± 0.7 vs 11 ± 1.3 km.h−1, p < 0.001). Kleg was similar between untrained and trained runners across each stage (15.8 ± 0.3 vs 14.3 ± 0.3 kN.m) and did not change between stages, yet spring-mass characteristics differed between groups. Vertical stiffness increased in the trained runners from pre-OBLA to post-OBLA (45.5 ± 3.35–51.9 ± 3.61 kN−1), but not in untrained runners (35.0 ± 5.2–39.6 ± 5.7 kN−1). Kleg was strongly related to Fpeak for trained runners only (r = 0.79; untrained runners, r = 0.34). Kleg was unaffected by physiological training status and was maintained across all OBLA stages. Trained runners appear to have optimised their spring-mass system in a homogenous manner, whilst less consistent spring-mass characteristics were observed in untrained runners. [ABSTRACT FROM AUTHOR]

The Abbotsford‐Sumas Aquifer is a permeable, unconfined aquifer in British Columbia, Canada, where raspberry (Rubus idaeus L.) production is an important source of groundwater NO3 contamination. Renovation of raspberry fields (i.e., canes chopped, soil tilled and fumigated, and spring manure application prior to replanting), which typically occurs every 6 to 10 yr in response to decreased crop vigor, has been suggested as a possible cause of significant interannual variation in groundwater NO3 concentrations. This study used high‐resolution passive diffusion sampling to quantify the magnitude and timing of NO3 loading to shallow groundwater from a commercial raspberry field during a 6‐yr (2009–2015) monitoring period after crop renovation. After renovation, the annual NO3 loading increased from ∼95 kg N ha−1 in Year 1 to ∼245 kg N ha−1 in Year 2 and decreased to ∼85 kg N ha−1 in Year 3. The average annual NO3 loading from Years 4 to 6 (72 kg N ha−1) was assumed to reflect annual loading without a renovation effect, and the increased loading during Years 1 to 3 was attributed to renovation. Renovation contributed an estimated 33 to 23% of total groundwater NO3 from this field for a 6‐ to 10‐yr renovation cycle. Most of the NO3 loading associated with renovation occurred in Year 2 and was attributed to the manure application. The increased NO3 loading after renovation likely contributes to the spatial and temporally varying NO3 patterns observed in the aquifer. Reducing manure applications during renovation and decreasing renovation frequency have the potential to decrease the groundwater NO3 concentration. Core Ideas: We estimated NO3 flux using vertically high‐resolution groundwater sampling and Darcy flux.Loading was estimated under raspberry field for 6 yr after stand renovation.Renovation frequency is an important control on long‐term NO3 concentration.An estimated one‐third to one‐quarter of groundwater NO3 was from renovation in a 6‐ to 10‐yr cycle.Manure was potentially the primary source of additional NO3 loading during renovation. [ABSTRACT FROM AUTHOR]

A rapid, high‐resolution shipboard survey, using a combination of lowered and expendable hydrographic measurements and vessel‐mounted acoustic Doppler current profiler data, provided a unique three‐dimensional view of an Arctic anti‐cyclonic cold‐core eddy. The eddy was situated 50‐km seaward of the Chukchi Sea shelfbreak over the 1,000 m isobath, embedded in the offshore side of the Chukchi slope current. The eddy core, centered near 150‐m depth, consisted of newly ventilated Pacific winter water which was high in nitrate and dissolved oxygen. Its fluorescence signal was due to phaeopigments rather than chlorophyll, indicating that photosynthesis was no longer active, consistent with an eddy age on the order of months. Subtracting out the slope current signal demonstrated that the eddy velocity field was symmetrical with a peak azimuthal speed of order 10 cm s−1. Its Rossby number was ~0.4, consistent with the fact that the measured cyclogeostrophic velocity was dominated by the geostrophic component. Different scenarios are discussed regarding how the eddy became embedded in the slope current, and what the associated ramifications are with respect to eddy spin‐down and ventilation of the Canada Basin halocline. Plain Language Summary: A critical feature of the interior Arctic Ocean is the sharp vertical change in salinity between roughly 100‐m to 200‐m depth, known as the cold halocline. This shields the warm Atlantic‐origin water below from mixing upward to the surface and melting the pack ice. The cold halocline is believed to be partially maintained by eddies of cold water emanating from the Chukchi Sea continental shelf. This paper presents measurements from a rapid, high‐resolution shipboard survey of a cold‐core Arctic eddy offshore of the shelf edge, providing a unique three‐dimensional view of the feature. The eddy's core contained water near the freezing point with a high level of nitrate, but the biological activity had largely ceased because the eddy had descended below the part of the water column exposed to sunlight. The eddy was imbedded in the offshore edge of the westward‐flowing Chukchi slope current. Different scenarios are discussed regarding how the eddy became embedded in the slope current, and what the associated ramifications are with respect to disintegration of the eddy and the manner in which the cold water feeds the halocline. Key Points: A rapid, high‐resolution shipboard survey provided a unique 3‐D view of an Arctic anti‐cyclonic cold‐core eddy on the Chukchi slopeThe winter water in the core contained high levels of nitrate, but the biological activity had largely ceased due to lack of sunlightThe eddy was imbedded in the seaward edge of the Chukchi slope current and likely formed in the vicinity of Barrow Canyon [ABSTRACT FROM AUTHOR]

Background: Following transfusion, donor white blood cells (WBCs) can persist long-term in the recipient, a phenomenon termed transfusion-associated microchimerism (TA-MC). Prior studies suggest TA-MC is limited to transfusion following traumatic injury, and is not prevented by leukoreduction.Study Design and Methods: We conducted a prospective cohort study at a major trauma center to evaluate TA-MC following injury. Index samples were collected upon arrival, prior to transfusion. Follow-up samples were collected at intervals up to one year, and beyond for those testing positive for TA-MC. TA-MC was detected by real-time quantitative allele-specific polymerase chain reaction assays at the HLA-DR locus and several polymorphic insertion deletion sites screening for non-recipient alleles.Results: A total of 378 trauma patients were enrolled (324 transfused cases and 54 non-transfused controls). Mean age was 42 ± 18 years, 74% were male, and 80% were injured by blunt mechanism. Mean Injury Severity Score was 20 ± 12. Among transfused patients, the median (interquartile range) number of red cell units transfused was 6 (3,12), and median time to first transfusion was 9 (0.8,45) hours. Only one case of long-term TA-MC was confirmed in our cohort. We detected short-term TA-MC in 6.5% of transfused subjects and 5.6% on non-transfused controls.Conclusions: In contrast to earlier studies, persistent TA-MC was not observed in our cohort of trauma subjects. Short-term TA-MC was detected, but at a lower frequency than previously observed, and rates were not significantly different than what was observed in non-transfused controls. The reduction in TA-MC occurrence may be attributable to changes in leukoreduction or other blood processing methods. [ABSTRACT FROM AUTHOR]

As human activities alter environmental conditions, the emergence and spread of disease represents an increasing threat to wildlife. Studies that examine how host-pathogen relationships play out across seasons and latitudes can serve as proxies for understanding how natural and anthropogenic changes in climate may influence infection and disease dynamics. Amphibians are ideal host organisms for studying the impacts of climate on disease because they are ectothermic and threatened by chytridiomycosis, a recently emerged and globally important disease caused by fungal pathogens in the genus Batrachochytrium. Previous studies suggest that temperature affects the interaction between amphibians and Batrachochytrium pathogens. However, a clearer understanding of this host-pathogen-environment interaction is needed to predict how the risk of chytridiomycosis will vary in space and time. Here, we investigate how daily, seasonal, and latitudinal variations in temperature affect the incidence and impact of Batrachochytrium dendrobatidis (Bd) infection in a broadly distributed host, the northern cricket frog (Acris crepitans), using a combination of field and laboratory studies. In a four-year field study conducted at three latitudes, we found that daily maximum air temperature over a 15-d period prior to sampling best predicted patterns of Bd infection and that the lightest infection loads followed periods when these temperatures exceeded 25°C. In a laboratory exposure experiment, we found pathogen load and mortality to be greater at temperatures that mimic winter temperatures at the southern extent of this host's range than for scenarios that mimic temperature conditions experienced in other areas and seasons. Taken together, our findings suggest that changes in temperature across timescales and latitudes interact to influence the dynamics of infection and disease in temperate amphibians. [ABSTRACT FROM AUTHOR]

System of interest (SoI) failures can sometimes be traced to an unexpected behavior occurring within another system that is a member of the system of systems (SoS) with the SoI. This article presents a method for use when designing an SoI that helps to analyze an SoS for unexpected behaviors from existing SoS members during the SoI's conceptual functional modeling phase of system architecture. The concept of irrationality initiators—unanticipated or unexpected failure flows emitted from one system that adversely impact an SoI, which appear to be impossible or irrational to engineers developing the new system—is introduced and implemented in a quantitative risk analysis method. The method is implemented in the failure flow identification and propagation framework to yield a probability distribution of failure paths through an SoI in the SoS. An example of a network of autonomous vehicles operating in a partially denied environment is presented to demonstrate the method. The method presented in this paper allows practitioners to more easily identify potential failure paths and prioritize fixing vulnerabilities in an SoI during functional modeling when significant changes can still be made with minimal impact to cost and schedule. [ABSTRACT FROM AUTHOR]

The quantity and quality of surface water are inherently connected yet are overwhelmingly studied separately in the field of remote sensing. Remotely observable water quantity (e.g., water extent, water elevation, lake/reservoir volume, and river discharge) and water quality (e.g., color, turbidity, total suspended solids, chlorophyll a, colored dissolved organic matter, and temperature) parameters of inland waterbodies interact through a series of hydrological and biogeochemical processes. In this review, we analyzed trends in remote sensing publications to understand the prevalence of studies on the quantity versus quality of open‐surface inland waterbodies (rivers, streams, lakes, and reservoirs) as well as identified opportunities for integrating both water quality and quantity sensing in future work. Our bibliometric analysis found that despite the increasing number of publications using remote sensing for inland waterbodies, few studies to date have used remote sensing tools or approaches to simultaneously study water quantity and quality. Ultimately, by providing insights into potential integration of the water quality and quantity studies, we aim to identify a pathway to advance the understanding of inland water dynamics and freshwater resources through remote sensing. This article is categorized under:Water and Life > MethodsScience of Water > Water QualityWater and Life > Nature of Freshwater EcosystemsScience of Water > Methods [ABSTRACT FROM AUTHOR]

Background: Whether stitching the palate during intraoral digital scans of implants would improve, scanning accuracy is unclear. Purpose: Evaluate the effect of stitching the palate and the scan body position on the trueness (distance and angular deviation) and precision of digital scans in a completely edentulous situation. Materials and Methods: An edentulous maxillary model with four parallel dental implant analogs was fabricated and intraoral scan bodies were attached. The entire surface was scanned using an industrial scanner to generate a master reference model digital scan (MRM‐DS). Digital scans of the master model were made using an intraoral scanner and the resulting scans were divided into two groups [stitched palate (S) and unstitched palate (U)]. All test scans were converted to STL files and superimposed over the MRM‐DS. Results: For trueness, scan body position had a significant effect on distance (P < .001) and angular (P < .001) deviation values. In terms of precision, no significant difference was found in distance (P =.051) and angular deviations (P =.36) between stitched and unstitched techniques. Conclusions: The accuracy and precision of digital scans of edentulous maxillary arch was similar independent of stitching or unstitching the palate. Position of the implant had a significant effect on trueness. [ABSTRACT FROM AUTHOR]

New Findings: What is the central question of this study?What is the distribution of the hyperaemic response to passive leg movement (PLM) in the common (CFA), deep (DFA) and superficial (SFA) femoral arteries? What is the impact of lower leg cuff‐induced blood flow occlusion on this response?What is the main finding and its importance?Of the total blood that passed through the CFA, the majority was directed to the DFA and this was unaffected by cuffing. As a small fraction does pass through the SFA to the lower leg, cuffing during PLM should be considered to emphasize the thigh‐specific hyperaemia. It has yet to be quantified how passive leg movement (PLM)‐induced hyperaemia, an index of vascular function, is distributed beyond the common femoral artery (CFA), into the deep femoral (DFA) and the superficial femoral (SFA) arteries, which supply blood to the thigh and lower leg, respectively. Furthermore, the impact of cuffing the lower leg, a common practice, especially with drug infusions during PLM, on the hyperaemic response is, also, unknown. Therefore, PLM was performed with and without cuff‐induced blood flow (BF) occlusion to the lower leg in 10 healthy subjects, with BF assessed by Doppler ultrasound. In terms of BF distribution during PLM, of the 380 ± 191 ml of blood that passed through the CFA, 69 ± 8% was directed to the DFA, while only 31 ± 8% passed through the SFA. Cuff occlusion of the lower leg significantly attenuated the PLM‐induced hyperaemia through the SFA (∼30%), which was reflected by a fall in BF through the CFA (∼20%), but not through the DFA. Additionally, cuff occlusion significantly attenuated the PLM‐induced peak change in BF (BFΔpeak) in the SFA (324 ± 159 to 214 ± 114 ml min−1), which was, again, reflected in the CFA (1019 ± 438 to 833 ± 476 ml min−1), but not in the DFA. Thus, the PLM‐induced hyperaemia predominantly passes through the DFA and this was unaltered by cuffing. However, as a small fraction of the PLM‐induced hyperaemia does pass through the SFA to the lower leg, cuffing the lower leg during PLM should be considered to emphasize thigh‐specific hyperaemia in the PLM assessment of vascular function. [ABSTRACT FROM AUTHOR]

Recent field experiments in Iceland and Washington State (USA) show that basalt formations may be favorable targets for carbon capture and sequestration (CCS) because CO2 mineralization reactions proceed rapidly. These results imply that there is tremendous opportunity for implementing CCS in large igneous provinces. However, the magnitude of this opportunity comprises commensurate levels of uncertainty because basalt reservoirs are characterized by highly heterogeneous, fracture‐controlled hydraulic properties. This geologic uncertainty is propagated as parametric uncertainty in quantitative risk models, thus limiting the efficacy of models to predict CCS performance attributes, such as reservoir integrity and storage potential. To overcome these limitations, this study presents a stochastic approach for quantifying the geomechanical performance attributes of CCS operations in a highly heterogeneous basalt reservoir. We utilize geostatistical reservoir characterization to develop an ensemble of equally probable permeability distributions in a flood basalt reservoir with characteristics of the Wallula Basalt Pilot Project. We then simulate industrial‐scale CO2 injections within the ensemble and calculate the mean and variance of fluid pressure over a 1‐year injection period. These calculations are combined with the state of stress in southeast Washington State to constrain the spatial extent at which shear failure, fracture initiation, and borehole breakdown may occur. Results from this study show that (i) permeability uncertainty alone causes injection pressure to vary over 25 MPa, (ii) shear failure is likely to occur at 7 times greater distances from the injection than the CO2 migrates, and (iii) joint initiation pressures are localized within the volume comprising the CO2 plume. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Aims: The pathological features and diagnostic reliability of crypt cell atypia CCA arising in inflammatory bowel disease IBD and its clinical significance are unknown. Methods and results: DNA flow cytometry FCM was performed on 14 colon biopsies of CCA from seven IBD patients male‐to‐female ratio, 5:2; mean age, 53 years; mean IBD duration, 15 years using paraffin‐embedded tissue. Seven gastrointestinal pathologists were asked to diagnose each biopsy as negative for dysplasia NEG, indefinite for dysplasia IND, low‐grade dysplasia LGD or high‐grade dysplasia HGD by morphology alone, then again with knowledge of FCM results. Aneuploidy was detected in all 14 biopsies, and five of eight biopsies 63% also showed strong and diffuse nuclear staining for p53 in the areas of CCA. Six 86% patients developed HGD n = 5 or adenocarcinoma n = 1 in the same colonic segment where CCA had been diagnosed within a mean follow‐up time of 27 months. No follow‐up information was available in the remaining one patient. When diagnoses were grouped as NEG or 'atypical' including IND, LGD or HGD, the overall agreement rate of 76% kappa = 0.51 based on morphology alone improved to 90% kappa = 0.81 with knowledge of FCM results. Even when categorised as NEG or dysplasia LGD or HGD with each of the IND diagnoses reclassified into three categories NEG, LGD or HGD based on the degree of suspicion for dysplasia, the overall agreement rate of 63% kappa = 0.25 based on morphology alone improved to 73% kappa = 0.46 with knowledge of FCM results. However, when grouped as NEG, LGD or HGD, the overall agreement rate was less than 40% kappa < 0.09 regardless of knowledge of FCM results. Conclusions: The presence of aneuploidy, p53 positivity and development of HGD or adenocarcinoma on follow‐up indicate that CCA likely represents a dysplastic lesion at least LGD and is a histological marker of neoplastic progression. Although the grading of CCA, largely based on cytological abnormalities, is subject to significant interobserver variability, CCA can be histologically identified and should lead to a recommendation of increased endoscopic surveillance, especially if aneuploidy is detected. [ABSTRACT FROM AUTHOR]

Background: Universal tumor testing for defective DNA mismatch repair (MMR) is recommended for all women diagnosed with endometrial cancer to identify those with underlying Lynch syndrome. However, the effectiveness of these screening methods in identifying individuals with Lynch syndrome across the population has not been well studied. The aim of this study was to evaluate outcomes of MMR immunohistochemistry (IHC), mutL homolog 1 (MLH1) methylation, and microsatellite instability (MSI) analysis among patients with endometrial cancer.Methods: A complete systematic search of online databases (PubMed, EMBASE, MEDLINE, and the Cochrane Library) for 1990-2018 was performed. A DerSimonian-Laird random effects model meta-analysis was used to estimate the weighted prevalence of Lynch syndrome diagnoses.Results: The comprehensive search produced 4400 publications. Twenty-nine peer-reviewed studies met the inclusion criteria. Patients with endometrial cancer (n = 6649) were identified, and 206 (3%) were confirmed to have Lynch syndrome through germline genetic testing after positive universal tumor molecular screening. Among 5917 patients who underwent tumor IHC, 28% had abnormal staining. Among 3140 patients who underwent MSI analysis, 31% had MSI. Among patients with endometrial cancer, the weighted prevalence of Lynch syndrome germline mutations was 15% (95% confidence interval [CI], 11%-18%) with deficient IHC staining and 19% (95% CI, 13%-26%) with a positive MSI analysis. Among 1159 patients who exhibited a loss of MLH1 staining, 143 (13.7%) were found to be MLH1 methylation-negative among those who underwent methylation testing, and 32 demonstrated a germline MLH1 mutation (2.8% of all absent MLH1 staining cases and 22.4% of all MLH1 methylation-negative cases). Forty-three percent of patients with endometrial cancer who were diagnosed with Lynch syndrome via tumor typing would have been missed by family history-based screening alone.Conclusions: Despite the widespread implementation of universal tumor testing in endometrial cancer, data regarding testing results remain limited. This study provides predictive values that will help practitioners to evaluate abnormal results in the context of Lynch syndrome and aid them in patient counseling. [ABSTRACT FROM AUTHOR]

Signals emanating from the B‐cell receptor (BCR) promote proliferation and survival in diverse forms of B‐cell lymphoma. Precision medicine strategies targeting the BCR pathway have been generally effective in treating lymphoma, but often fail to produce durable responses in diffuse large B‐cell lymphoma (DLBCL), a common and aggressive cancer. New insights into DLBCL biology garnered from genomic analyses and functional proteogenomic studies have identified novel modes of BCR signaling in this disease. Herein, we describe the distinct roles of antigen‐dependent and antigen‐independent BCR signaling in different subtypes of DLBCL. We highlight mechanisms by which the BCR cooperates with TLR9 and mutant isoforms of MYD88 to drive sustained NF‐κB activity in the activated B‐cell‐like (ABC) subtype of DLBCL. Finally, we discuss progress in detecting and targeting oncogenic BCR signaling to improve the survival of patients with lymphoma. [ABSTRACT FROM AUTHOR]

Objectives: This paper tested two moderators, brooding and participant sex, on the respective relations between thwarted belongingness (TB), perceived burdensomeness (PB), and suicide risk. Method: Using a cross‐sectional design in a sample of undergraduates (N = 278), two hierarchical regression models examining the three‐way interaction between brooding, sex, and either TB or PB on suicide risk were conducted. Results: A significant two‐way interaction of brooding and TB was detected, but no moderating effect of sex was observed. There was a significant three‐way interaction of brooding, sex, and PB on suicide risk. Conclusions: High levels of TB and brooding produce increased suicide risk across sexes. The relation between PB and suicide risk is dependent on brooding for females but not males. [ABSTRACT FROM AUTHOR]

Background: Sunscreens, unlike prescription medications, are purchased by consumers directly from retailers. The proportion of online sunscreen sales is increasing. It is therefore important for dermatologists to know what factors influence online sunscreen purchases to optimize appropriate recommendations. Methods: Data on the top 100 best‐selling sunscreens from an online retailer were collected. Variables included cost, formulation, product claims, ingredients, consumer ratings, and number of reviews. Ordinal logistic regression was used to analyze the impact of collected variables on position on the best‐seller list. Results: Ninety‐six of the 100 search results could be defined as actual sunscreens with a total of 41 788 reviews. The median price per ounce was $3.02 (range $0.34‐$309.18). The most popular formulations were lotions. The most common unregulated claim was "non‐greasy" found in 57.3% of sunscreens. For 26 unregulated product claims analyzed, the mean number of claims per sunscreen was 5.2. Using an ordinal regression model, the following factors were found to significantly influence sunscreen sales: number of reviews, the claim "decreases the risk of skin cancer and early aging," and the presence of six or more unregulated claims. Conclusions: Multiple sunscreen options exist for consumers with varying price points, active ingredients, and formulations. Consumers who purchase online prefer sunscreens with a higher number of reviews and more unregulated marketing claims. FDA‐regulated claims such as "decreases the risk of skin cancer and early aging" are not as impactful in this purchasing cohort. To facilitate usage, dermatologists should be cognizant of factors that influence sunscreen selection among this group. [ABSTRACT FROM AUTHOR]

Rationale: Stable isotope analysis is used to understand the foraging habits and movements of a diverse set of organisms. Variability in stable isotope ratios among tissues derived from the same animal makes it difficult to compare data among study results in which different tissue types are evaluated. Isotopic relationships between two green turtle (Chelonia mydas) tissue types, skin and unhatched egg contents are unknown. Similarly, few data exist to evaluate the influence of time elapsed after oviposition (as a proxy for decomposition) on isotopic variability among unhatched eggs within the same nest. Methods: Skin and unhatched egg contents were collected from 69 adult female green turtles and associated nests at the Archie Carr National Wildlife Refuge in Florida, USA. Values of δ13C, δ15N, and δ34S were measured for both tissue types using a continuous flow isotope ratio mass spectrometer. Standardized major‐axis (SMA) regression was used to generate conversion equations of carbon, nitrogen, and sulfur isotope ratios between the two tissue types. Model selection frameworks consisting of single‐factor linear models were employed per isotope ratio to assess how egg time‐in‐nest affected intraclutch isotopic variability. Results: Conversion equations for all three isotope ratios indicated significant relationships between skin and unhatched egg values, although model fits were lower than found in some studies examining similar patterns in other marine turtle species. The probability of increased intraclutch variability was significantly higher among eggs collected at longer intervals after deposition. Conclusions: This study reports the first‐ever δ13C and δ15N conversion equations between skin and unhatched eggs for green turtles, and the first δ34S conversion equation for any marine turtle species. SMA regression was used to directly convert tissue values bidirectionally, unlike equations generated using ordinary least‐squares regression. Issues with increased intraclutch variability at later excavation dates highlight the importance of collecting unhatched eggs as soon as possible after hatchling emergence. [ABSTRACT FROM AUTHOR]

Individual with substance use disorders have well‐recognized impairments in cognitive control, including in behavioral adaptation after mistakes. One way in which this impairment manifests is via diminished post‐error slowing, the increase in reaction time following a task‐related error that is posited to reflect cautionary or corrective behavior. Yet, in the substance use disorder literature, findings with regard to post‐error slowing have been inconsistent, and thus could benefit from quantitative integration. Here, we conducted a meta‐analysis of case–control studies examining post‐error slowing in addiction. Twelve studies with 15 unique comparisons were identified, comprising 567 substance users and 384 healthy controls across three broad types of inhibitory control paradigms (go‐no/go, conflict resolution, and stop signal tasks, respectively). Results of the random‐effects meta‐analysis revealed a moderate group difference across all studies (Cohen's d = 0.31), such that the individuals with substance use disorder had diminished post‐error slowing compared with controls. Despite this omnibus effect, there was also large variability in the magnitude of the effects, explained in part by differences between studies in task complexity. These findings suggest that post‐error slowing may serve as a promising and easy‐to‐implement measure of cognitive control impairment in substance use disorder, with potential links to aberrant brain function in cognitive control areas such as the anterior cingulate cortex. [ABSTRACT FROM AUTHOR]

Mass bat exposures (MBEs) occur when multiple people are exposed to a bat or a bat colony, often over an extended period. In August 2017, a public health investigation was started in response to an MBE that occurred during May–August 2017 at a national park research station in Wyoming. We identified 176 people who had slept primarily in two lodges (Lodges A and B) at the research station, and successfully contacted 165 (93.8%) of these individuals. Risk assessments (RAs) were administered to all 165 individuals to determine degree and type of exposures to bats (e.g., biting or scratching). Exposure status for research station guests was classified as "non‐exposed," "low risk" or "high risk," and counselling was provided to guide post‐exposure prophylaxis (PEP) recommendations. Prior to public health notification and intervention, 19 persons made the decision to pursue PEP. The healthcare‐seeking behaviours of this group were taken to represent outcomes in the absence of public health intervention. (These persons received a RA, and their risk classification was retrospectively assigned.) Approximately 1–2 weeks after conducting the RAs, we conducted a follow‐up survey to determine whether recommendations regarding PEP were ultimately followed. The proportion of individuals that unnecessarily pursued PEP was higher among the 19 individuals that sought health care prior to receiving the RA (p < 0.00001). Among those receiving the RA first, all persons classified as high risk followed public health guidance to seek PEP treatment. Despite this, upon re‐interview, only 21/79 (26.6%) of guests could accurately recall their risk classification, with most people (55.7%) overestimating their risk. Study findings demonstrate that early public health interventions such as RAs can reduce unnecessary use of PEP and that messaging used during rabies counselling should be clear. [ABSTRACT FROM AUTHOR]

Nicotinic acetylcholine receptors (nAChRs), prototype members of the cys‐loop ligand‐gated ion channel family, are key mediators of cholinergic transmission in the central nervous system. Despite their importance, technical gaps exist in our ability to dissect the function of individual subunits in the brain. To overcome these barriers, we designed CRISPR/Cas9 small guide RNA sequences (sgRNAs) for the production of loss‐of‐function alleles in mouse nAChR genes. These sgRNAs were validated in vitro via deep sequencing. We subsequently targeted candidate nAChR genes in vivo by creating herpes simplex virus (HSV) vectors delivering sgRNAs and Cas9 expression to mouse brain. The production of loss‐of‐function insertions or deletions (indels) by these 'all‐in‐one' HSV vectors was confirmed using brain slice patch clamp electrophysiology coupled with pharmacological analysis. Next, we developed a scheme for cell type‐specific gene editing in mouse brain. Knockin mice expressing Cas9 in a Cre‐dependent manner were validated using viral microinjections and genetic crosses to common Cre‐driver mouse lines. We subsequently confirmed functional Cas9 activity by targeting the ubiquitous neuronal protein, NeuN, using adeno‐associated virus (AAV) delivery of sgRNAs. Finally, the mouse β2 nAChR gene was successfully targeted in dopamine transporter (DAT)‐positive neurons via CRISPR/Cas9. The sgRNA sequences and viral vectors, including our scheme for Cre‐dependent gene editing, should be generally useful to the scientific research community. These tools could lead to new discoveries related to the function of nAChRs in neurotransmission and behavioral processes. [ABSTRACT FROM AUTHOR]

Property O, introduced by Galkin et al. for arbitrary complex, Fano manifolds X, is a statement about the eigenvalues of the linear operator obtained by the quantum multiplication by the anticanonical class of X. We prove that property O holds in the case when X= IG (k,2n+1) is an odd symplectic Grassmannian. The proof uses the combinatorics of the recently found quantum Chevalley formula for IG (k,2n+1), together with the Perron–Frobenius theory of nonnegative matrices. [ABSTRACT FROM AUTHOR]

Model simulations of ozone (O3) driven by meteorological reanalyses are useful for filling observational gaps and interpreting observed O3 variability and trends. However, the transport circulation of reanalysis products is impacted by changes to the observing system (the data assimilated into the reanalyses). We examine the impacts of these changes on simulated O3 from two models, Global Modeling Initiative (GMI) Chemistry Transport Model (GMI CTM) and Modern‐Era Retrospective Analysis for Research Applications version 2 (MERRA‐2) GMI Replay (M2 GMI Replay) simulation, using observations from global ozonesondes (>50,000 profiles) and satellites from 1980 to 2016. Both models are constrained by meteorology from the NASA MERRA‐2 reanalysis, and both use versions of NASA's GMI chemical mechanism. We focus on an observing system change affecting simulated O3 after 1998, associated with the assimilation of temperature and humidity data from new microwave profiling satellites. A large post‐1998 O3 increase, mainly confined to 15–20‐km altitude, of ~10 Dobson units (DU) in midlatitudes occurs in the GMI CTM, worsening the bias compared to observations. In contrast, an increase in M2 GMI Replay simulation O3 of ~10 DU is observed only near −60° latitude, reducing the bias compared to observations. The GMI CTM O3 high biases display a Quasi‐Biennial Oscillation (QBO)‐like periodicity that result from excessive transport from the tropical stratosphere to the midlatitude lower stratosphere during the QBO westerly phase. We quantify O3 discontinuities caused by MERRA‐2 observing system changes and demonstrate how the MERRA‐2 Global Modeling Initiative Replay simulation dampens the effects of these changes and QBO‐driven artifacts on simulated lower stratospheric and total O3. We caution against using simulations driven by a reanalysis to derive multidecadal O3 trends, especially prior to 1998. Key Points: Ozonesonde and satellite observations are used to evaluate two 1980–2016 model simulations of O3 driven by MERRA‐2 meteorologyAn observing system change in 1998 that is known to affect MERRA‐2 meteorology causes major O3 discontinuities in both simulationsThe 1998 change impacts the stratosphere‐to‐troposphere O3 transport differently in the simulations, improving one and worsening the other [ABSTRACT FROM AUTHOR]

Objectives: Behavioral inhibition is a trait‐level factor associated with posttraumatic stress. Safety behaviors may impact this link by interfering with anxiety habituation. The current study examined the unique and interactive effects of behavioral inhibition, safety behaviors, and participant sex on posttrauma symptom clusters. Method: Participants (N = 131; 75.6% female; M = 19.9 years) completed a trauma history interview and questionnaires assessing behavioral inhibition, safety behavior, and posttrauma symptom severity. Results: Safety behaviors were associated with intrusion (partial correlations [pr] = 0.319), avoidance (pr = 0.274), cognition‐mood (pr = 0.274), and arousal–reactivity (pr = 0.538) symptoms (all p ≤ 0.001). An interaction of sex and safety behaviors was noted for avoidance (p = 0.047, pr = −0.159) with a significant relation observed only among women (p < 0.001, pr = 0.442). Safety behaviors also moderated the link between behavioral inhibition and arousal–reactivity (p = 0.002, pr = 0.272) with inhibition predicting symptoms at high levels of safety behavior (p = 0.024, pr = 0.171). Conclusion: Trauma‐related safety behaviors are associated with greater posttrauma symptoms and evidence differential effects across individual symptom domains. [ABSTRACT FROM AUTHOR]

Injecting supercritical CO2 into the subsurface changes the temperature, pressure, and geochemistry of the storage reservoir. Understanding these perturbations within the reservoir may be used to monitor the CO2 plume during a carbon capture and sequestration (CCS) project. Here we analyze results from 1‐D, 2‐D, and 3‐D numerical modeling studies to investigate how the thermal signature of the CO2‐water system evolves during CCS. These models show that the thermodynamic processes of the CO2‐water system results in a characteristic thermal profile within a homogeneous storage reservoir during a CO2 injection. This thermal signature is characterized by warming front of up to 4 °C, which is caused by CO2 dissolution and migrates contemporaneously with free‐phase CO2 migration. When reservoir properties are highly heterogeneous, this thermal front travels well ahead of free‐phase CO2, thus implying that thermal monitoring may be an effective predictor of CO2 breakthrough. Plain Language Summary: When CO2 is injected into a geologic reservoir, it causes the pressure, temperature, and chemical systems with the reservoir to change. Understanding these changes are important in order to plan a CO2 injection and monitor the CO2 during the injection. Monitoring CO2 in the subsurface is required to avoid negative health, safety, and environmental impacts. The temperature effects associated with a CO2 injection may be used as a way to monitor the CO2 plume during the injection phase. Results here show that the heat of dissolution can cause temperature to increase up to 4 °C and can be used as a way to predict the arrival of CO2. Key Points: Thermodynamic processes induce warming of 1‐4 °C at the leading edge of a CO2 plumeHeat and CO2 migrate contemporaneously within homogeneous reservoirsPositive thermal anomalies migrate weeks to years ahead of free‐phase CO2 in heterogeneous reservoirs [ABSTRACT FROM AUTHOR]

The su(var)3-9, enhancer of zeste, trithorax (SET)/inhibitor 2 of protein phosphatase 2A (PP2A) oncoprotein binds and inhibits PP2A, composed of various isoforms of scaffolding, regulatory, and catalytic subunits. Targeting SET with a sphingolipid analog drug fingolimod (FTY720) or ceramide leads to the reactivation of tumor suppressor PP2A. However, molecular details of the SET-FTY720 or SET-ceramide, and mechanism of FTY720-dependent PP2A activation, remain unknown. Here, we report the first in solution examination of the SET-FTY720 or SET-ceramide complexes by NMR spectroscopy. FTY720-ceramide binding resulted in chemical shifts of residues residing at the N terminus of SET, preventing its dimerization or oligomerization. This then released SET from PP2ACα, resulting in PP2A activation, while monomeric SET remained associated with the B56γ. Our data also suggest that the PP2A holoenzyme, composed of PP2A-Aβ, PP2A-B56γ, and PP2ACα subunits, is selectively activated in response to the formation of the SET-FTY720 complex in A549 cells. Various PP2A-associated downstream effector proteins in the presence or absence of FTY720 were then identified by stable isotope labeling with amino cells in cell culture, including tumor suppressor nonmuscle myosin IIA. Attenuation of FTY720-SET association by point mutations of residues that are involved in FTY720 binding or dephosphorylation of SET at Serine 171, enhanced SET oligomerization and the formation of the SET-PP2A inhibitory complex, leading to resistance to FTY720-dependent PP2A activation. [ABSTRACT FROM AUTHOR]

Objectives: An adverse event (AE) is a physical harm experienced by a patient due to health care, requiring intervention. Describing and categorizing AEs is important for quality and safety assessment and identifying areas for improvement. Safety science suggests that improvement efforts should focus on preventing and mitigating harm rather than on error, which is commonplace but infrequently leads to AEs. Most taxonomies fail to describe harm experienced by patients (e.g., hypoxia, hemorrhage, anaphylaxis), focusing instead on errors, and use categorizations that are too broad to be useful (e.g., "communication error"). We set out to create a patient‐centered, emergency department (ED)‐specific framework for describing AEs and near misses to advance quality and safety in the acute care setting. Methods: We performed a critical review of existing taxonomies of harm, evaluating their applicability to the ED. We identified and adopted a classification framework and developed a taxonomy using an iterative process categorizing approximately 600 previously identified AEs and near misses. We reviewed this taxonomy with collaborators at four medical centers, receiving feedback and providing clarification. We then disseminated a set of representative scenarios for these safety experts to categorize independently using the taxonomy. We calculated interrater reliability and performance compared to our criterion standard. Results: Our search identified candidate taxonomies for detailed review. We selected the Adventist Health Systems AE taxonomy and modified this for use in the ED, adopting a framework of categories, subcategories, and up to three modifiers to further describe events. On testing, overall reviewer agreement with the criterion standard was 92% at the category level and 88% at the subcategory level. Three of the four raters concurred in 55 of 59 scenarios (93%) and all four concurred in 46 of 59 scenarios (78%). At the subcategory level, there was complete agreement in 40 of 59 (68%) scenarios and majority agreement in 55 of 59 instances (93%). Performance of individual raters ranged from very good (88%, 52/59) to near perfect (98%, 58/59) at the main category level. Conclusions: We developed a taxonomy of AEs and near misses for the ED, modified from an existing framework. Testing of the tool with minimal training yielded high performance and good inter‐rater reliability. This taxonomy can be adapted and modified by EDs seeking to enhance their quality and safety reviews and characterize harm occurring in their EDs for quality improvement purposes. [ABSTRACT FROM AUTHOR]

Summary: Background: Insufficient understanding of sunscreen labeling terminology is a barrier to effective use. The Food and Drug Administration (FDA) issued the "final rule" on sunscreen labeling in 2011, in an effort to promote effective usage. However, relatively little is known about patient knowledge of sunscreen labeling terminology. This study assesses the sunscreen labeling knowledge of dermatology patients, with an emphasis on understanding of the FDA‐mandated wording. Methods: A validated survey was administered to consecutive dermatology office patients. Respondents answered questions about sunscreen use practices, sunscreen knowledge, and demographics. To assess their sunscreen knowledge, they responded to questions on the concepts of sun protection factor, broad‐spectrum, and waterproof. Results: A total of 334 patients completed surveys. Only 8.7% of patients correctly answered all three questions related to sunscreen labeling terminology. Patients with a personal history of skin cancer were more likely to answer more than half of the questions correctly (P = 0.004). Older persons and those with darker skin types were most likely to answer all questions incorrectly. Conclusion: General understanding of sunscreen labeling was poor, and a minority of consumers comprehended the key features of sunscreen labeling. This knowledge gap appeared to be slightly smaller in the subpopulation of patients with a personal history of skin cancer. [ABSTRACT FROM AUTHOR]

Iron is a common component of granular fertilizers. Chelating agents are commonly used to increase Fe solubility and plant uptake. Numerous products are marketed as Fe chelates but few have been confirmed to increase Fe solubility in soils. The objective of this study was to determine the solubility of Fe from 10 common Fe fertilizers applied to 11 North American soils. Brookston, Cecil, Fuquay, Hallandale, Marietta, Marvyn, Nunn, Pinavetes, Stephenville, Troup, and Zook soils were incubated with soluble Fe applied as sulfate; glucoheptonate; polysaccharide; humate; oxide; citrate; 1,2 dicarboxyethyl D,L aspartic acid (IDHA); ethylenediaminetetraacetic acid (EDTA); diethylenetriaminepentaacetic acid (DTPA); and ethylenediamine- o-hydroxyphenylacetic acid (EDDHA). At 1 h, 1 d, 1 wk, 2 wk, and 3 wk, soils were extracted with 0.01 mol L-1 CaCl2 and analyzed for Fe. In each soil 1 h after application, greater than 95% of applied Fe was rendered insoluble from sulfate, glucoheptonate, polysaccharide, humate, and oxide. The chelates FeEDTA, FeDTPA, and FeEDDHA resulted in increased Fe solubility compared with untreated soil within each sampling time in most soils with Fe solubility at 21 d ranging from 5 to 40% of applied Fe. The natural organic chelate (NOC), FeIDHA, increased Fe solubility in 3 of the 11 soils for 1 d, but soluble Fe from FeIDHA declined to that of untreated soils thereafter. These results suggest that soil-applied Fe fertilizers should be limited to EDTA, DTPA, or EDDHA. [ABSTRACT FROM AUTHOR]

Summary: Complex II [succinate dehydrogenase (succinate‐ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5–SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1–SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1–SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species‐dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate‐dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility. Significance Statement: Mitochondrial complex II is the only component shared between the electron transport chain and the tricarboxylic acid (TCA) cycle, and it plays a key role in respiration, metabolism and signaling in stress and pathogen responses in plants. [ABSTRACT FROM AUTHOR]

In this study, field‐aligned currents (FACs) and ionospheric electric fields on different spatial scales are investigated through the analysis of FAC data from the Swarm satellites and electric field data from the Dynamic Explorer 2, respectively, from all seasons and under all solar wind conditions and varying levels of solar activity. Distributions of the average and variable components of FAC and electric field are the main focuses of this study, where the FAC variability is represented by the standard deviation of FAC in each magnetic latitude/magnetic local time bin and electric field variability is represented by the square root of the sum of squares of standard deviations of magnetic eastward and equatorward components of the electric field. We found that the mean patterns of the FAC and electric field are mainly contributed by the large‐scale (wavelength: ⩾500 km) FAC and electric field. Unlike the average, in addition to the large scale, variabilities of FAC and electric field are not negligible on mesoscale (wavelength: 100–500 km) and small scale (wavelength: 8–100 km), while the FAC variability shows a different scale dependence from the electric field variability. Specifically, for decreasing scale sizes, the FAC variability increases while the electric field variability decreases, suggesting that the strong FACs on small scale and mesoscale do not necessarily correspond to strong ionospheric electric fields on those scales. Further, FAC variabilities on large scale and mesoscale are included into the Global Ionosphere Thermosphere Model (GITM) and the corresponding impacts on Joule heating have been assessed. It was found that, for the conditions studied here, the large‐scale FAC variability may significantly increase the Joule heating (~160% globally) and that the enhancement due to the mesoscale FAC variability is not negligible (~36% globally). Key Points: FACs and ionospheric electric fields are investigated on different scalesStrong FACs on small scale and mesoscale do not necessarily correspond to strong ionospheric electric fields on those scalesThe Joule heating can be enhanced by ~36% associated with the mesoscale FAC variability for the conditions studied here [ABSTRACT FROM AUTHOR]

Objective: Esthesioneuroblastoma is an uncommon malignancy of the sinonasal tract arising from the olfactory epithelium. Surgical management of the primary site, often via an endoscopic approach, with or without adjuvant radiation, is often curative. There is growing but ultimately limited data regarding management of the neck and the risk of nodal metastases. In this study, we examine the incidence and patterns of esthesioneuroblastoma-related cervical nodal metastases using the Surveillance, Epidemiology, and End Results (SEER) database.Methods: The SEER registry was queried for all patients with esthesioneuroblastomas diagnosed between 1973 and 2012. Patient data was then analyzed with respect to age, sex, race, modified Kadish stage, grade, survival functions, and nodal disease including specific nodal basins.Results: Three hundred and eighty-one cases of esthesioneuroblastoma with information on nodal metastases were identified. The overall cervical nodal metastasis rate was 8.7%. Level II metastases were most common (6.6%). A total of 4.5% of cases presented with multiple positive nodal basins. Male sex (P = 0.009) and higher tumor grade (P = 0.009) correlated with the presence of level II metastases. There was no association of primary tumor site to the presence of nodal metastases (P > 0.05). The presence of nodal disease significantly predicted poorer overall (P = 0.001) and disease-specific survival (P = 0.017).Conclusion: The incidence of nodal metastases in esthesioneuroblastoma at diagnosis is rare, and elective management of the neck remains controversial. Primary tumor site does not appear to predict metastases at specific nodal basins. Higher tumor grade may be a harbinger of eventual nodal metastases.Level Of Evidence: NA Laryngoscope, 129:1025-1029, 2019. [ABSTRACT FROM AUTHOR]

Objective: Childhood bereavement is linked to suicide‐related behaviors in adolescence and adulthood, but candidate mechanisms through which bereavement may lead to suicide‐related behaviors have not been explored. One candidate pathway is that grief reaction arising from bereavement lead to increased perceived burdensomeness and/or thwarted belongingness, resulting in increased suicide ideation. This cross‐sectional study of bereaved adolescents explored indirect effects between grief reactions as distal predictors, perceived burdensomeness and thwarted belongingness as proximal predictors, and suicide ideation. Method: Participants were 58 bereaved youth, 12–17 years of age (mean = 14.21, SD = 1.65; 81.0% female; 51.7% Hispanic, 17.2% African American, and 22.4% Caucasian), and their parents/guardians seeking services at a trauma and grief specialty outpatient clinic. Results: The indirect effect of grief reactions on suicide ideation via thwarted belongingness, but not perceived burdensomeness, was statistically significant. Conclusions: Clinicians may wish to consider signs of thwarted belongingness as possible indicators of suicide risk among bereaved youth. [ABSTRACT FROM AUTHOR]

The identification of theoretically and empirically supported correlates of suicide ideation is important to improve treatment approaches to suicide. This study sought to examine the association between interpersonal trust (theoretically conceptualized as a distal risk marker) and suicide ideation in adolescence. Specifically, it was hypothesized that interpersonal trust would be negatively associated with suicide ideation via perceived burdensomeness and thwarted belongingness (conceptualized as more proximal risk markers). Data were drawn from a cross-sectional sample of 387 adolescent inpatients between the ages of 12 and 17 years (M = 14.72, SD = 1.49). The sample was 63.6% female, 37.5% Hispanic, 26.9% African American/Black, and 25.8% Caucasian. Adolescents completed a series of self-report measures to assess thwarted belongingness, perceived burdensomeness, interpersonal trust, depressive symptoms, and suicide ideation. A structural equation model was fit to the data, and results demonstrated a significant indirect path from interpersonal trust to suicide ideation via perceived burdensomeness, but not thwarted belongingness. Results suggest that interpersonal trust may be a distal risk marker for suicide ideation and that interventions to increase interpersonal trust may help prevent the development of thwarted belongingness, perceived burdensomeness, and suicide ideation. [ABSTRACT FROM AUTHOR]

Rationale: Biochar amendments often decrease N2O gas production from soil, but the mechanisms and magnitudes are still not well characterized since N2O can be produced via several different microbial pathways. We evaluated the influence of biochar amendment on N2O emissions and N2O isotopic composition, including 15N site preference (SP) under anaerobic conditions. Methods: An agricultural soil was incubated with differing levels of biochar. Incubations were conducted under anaerobic conditions for 10 days with and without acetylene, which inhibits N2O reduction to N2. The N2O concentrations were measured every 2 days, the SPs were determined after 5 days of incubation, and the inorganic nitrogen concentrations were measured after the incubation. Results: The SP values with acetylene were consistent with N2O production by bacterial denitrification and those without acetylene were consistent with bacterial denitrification that included N2O reduction to N2. There was no effect of biochar on N2O production in the presence of acetylene between day 3 and day 10. However, in the absence of acetylene, soils incubated with 4% biochar produced less N2O than soils with no biochar addition. Different amounts of biochar amendment did not change the SP values. Conclusions: Our study used N2O emission rates and SP values to understand biochar amendment mechanisms and demonstrated that biochar amendment reduces N2O emissions by stimulating the last step of denitrification. It also suggested a possible shift in N2O‐reducing microbial taxa in 4% biochar samples. [ABSTRACT FROM AUTHOR]

Core Ideas: NO3 leaching was quantified by passive capillary wick samplers over 2 yr.NO3 leaching was three times greater in Year 1 than Year 2 (240 vs. 80 kg N ha−1).Increased Year 1 NO3 leaching reflected field renovation prior to monitoring period.There was strong seasonality in NO3 leaching from the field.Despite fertilizer banding in rows, 60% of NO3 leached from alleys between rows. Groundwater NO3–N contamination in the Abbotsford‐Sumas Aquifer in British Columbia, Canada, has been attributed primarily to NO3–N leaching from red raspberry (Rubus idaeus L.); however, direct estimates of NO3–N leaching are lacking. This study quantified the magnitude and timing of NO3–N leaching under a commercial red raspberry field over 30 mo (October 2010–March 2013) using passive capillary wick samplers installed below the root zone at three row locations (irrigated row, nonirrigated row, and alley) after the critical period of field renovation and replanting. Substantial NO3–N leaching (240 kg N ha−1) during the first year of monitoring was attributed to the effects of field renovation (including autumn chopping and incorporation of raspberry canes and soil fumigation and spring poultry broiler manure application) in the year prior to the initiation of monitoring. Lower NO3–N leaching (80 kg N ha−1) occurred in the second year of monitoring under typical mineral fertilizer management practices. Strong seasonality of NO3–N leaching was observed in both years, with ∼48% in autumn, 34% in spring and summer, and 17% in winter. Approximately 60% of the NO3–N leaching was attributed to the alleys between raspberry rows, which did not receive mineral fertilizer or irrigation. The high proportion of leaching during spring and summer and from the alleys suggests that growing‐season irrigation practices and alley vegetation management, respectively, would be good targets for the development of improved practices. The samplers were effective in quantifying the magnitude and timing of NO3–N leaching from a commercial agricultural field and informing the development of improved practices. [ABSTRACT FROM AUTHOR]

Objectives: Research demonstrates consistent relations between posttrauma symptoms and interpersonal dysfunction. The current study examined the extent to which perceptions of community rejection account for the relation between posttraumatic stress disorder (PTSD) and interpersonal outcomes in students exposed to assaultive and nonassaultive trauma. Method: Participants (N = 137; 66.4% female; M = 20 years) completed a trauma history interview, questionnaires assessing symptom severity, social disapproval, and interpersonal outcomes. Results: Assault survivors (n = 83) reported greater symptoms (d = 0.57), disapproval (d = 0.80), and social impairment (d = 0.51) relative to the nonassault group (n = 54). However, regression analyses indicated stronger associations between PTSD and community disapproval in survivors of nonassaultive (β = 0.69; p < 0.001) versus assaultive (β = 0.34; p < 0.001) events. Indirect effects of PTSD on perceived support and interpersonal functioning through social disapproval were also larger for nonassaultive versus assaultive groups (p < 0.05). Conclusion: Despite greater dysfunction among assault survivors, perceptions of disapproval may be a more salient factor for interpersonal dynamics following nonassaultive trauma. [ABSTRACT FROM AUTHOR]

Idiopathic non‐cirrhotic portal hypertension (INCPH) is a rare vascular liver disease that has attracted new interest in recent years. It is characterised by clinical signs of portal hypertension in the absence of cirrhosis or severe fibrosis and any known cause of portal hypertension. As much uncertainty exists about INCPH pathophysiology, and no definite diagnostic tests are available, liver biopsy is an essential tool for achieving a definite diagnosis. Unfortunately, the histological diagnosis of INCPH is not always straightforward, as the characteristic lesions are unevenly distributed, vary greatly in their severity, are often very subtle, and are not all necessarily present in a single case. Furthermore, specifically for the characteristic portal vessel changes observed in INCPH, the terminology and definition are ambiguous, which adds complexity to the already complex clinicopathological scenario. An international study group of liver pathologists and hepatologists pursued a consensus on nomenclature for the portal vascular lesions of INCPH. Such standardisation may assist pathologists in the recognition of such lesions, and will possibly facilitate further advancement in this field. [ABSTRACT FROM AUTHOR]

Retinal ganglion cells (RGCs) are tasked with transmitting all light information from the eye to the retinal recipient areas of the brain. RGCs can be classified into many different types by morphology, gene expression, axonal projections, and functional responses to different light stimuli. Ultimately, these classification systems should be unified into an all‐encompassing taxonomy. Toward that end, we show here that nearly all RGCs express either Islet‐2 (Isl2), Tbr2, or a combination of Satb1 and Satb2. We present gene expression data supporting the hypothesis that Satb1 and Satb2 are expressed in ON‐OFF direction‐selective (DS) RGCs, complementing our previous work demonstrating that RGCs that express Isl2 and Tbr2 are non‐DS and non‐image‐forming, respectively. Expression of these transcription factors emerges at distinct embryonic ages and only in postmitotic cells. Finally, we demonstrate that these transcription factor‐defined RGC classes are born throughout RGC genesis. The authors show that in the mouse, retinal ganglion cells can be divided into three classes, each expressing just one of the transcription factors: Isl2, Tbr2 and SatB1 or SatB2. These classes emerge post‐mitotically and cells from each class are born over highly overlapped periods of retinal development. [ABSTRACT FROM AUTHOR]