1. Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons.
- Author
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Kenney, Justin W., Genheden, Maja, Moon, Kyung ‐ Mee, Wang, Xuemin, Foster, Leonard J., and Proud, Christopher G.
- Subjects
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ELONGATION factors (Biochemistry) , *TUBULINS , *PROTEIN synthesis , *AMINO acids , *GENE ontology - Abstract
Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in both neurons and other cell types. Elongation is primarily regulated via eukaryotic elongation factor 2 kinase ( eEF2K). However, the consequence of altering eEF2K activity on the synthesis of specific proteins is largely unknown. Using both pharmacological and genetic manipulations of eEF2K combined with two protein-labeling techniques, stable isotope labeling of amino acids in cell culture and bio-orthogonal non-canonical amino acid tagging, we identified a subset of proteins whose synthesis is sensitive to inhibition of eEF2K in murine primary cortical neurons. Gene ontology ( GO) analyses indicated that processes related to microtubules are particularly sensitive to eEF2K inhibition. Our findings suggest that eEF2K likely contributes to neuronal function by regulating the synthesis of microtubule-related proteins. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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