1. D- AKAP1a is a signal-anchored protein in the mitochondrial outer membrane.
- Author
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Jun, Yong-Woo, Park, Heeju, Lee, You-Kyung, Kaang, Bong-Kiun, Lee, Jin-A, and Jang, Deok-Jin
- Subjects
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MITOCHONDRIAL proteins , *MITOCHONDRIAL membranes , *MEMBRANE proteins , *CELLULAR signal transduction , *PROTEIN structure - Abstract
Dual A-kinase anchoring protein 1a (D- AKAP1a, AKAP1) regulates cAMP signaling in mitochondria. However, it is not clear how D- AKAP1a is associated with mitochondria. In this study, we show that D- AKAP1a is a transmembrane protein in the mitochondrial outer membrane ( MOM). We revealed that the N-terminus of D- AKAP1a is exposed to the intermembrane space of mitochondria and that its C-terminus is located on the cytoplasmic side of the MOM. Moderate hydrophobicity and the positively charged flanking residues of the transmembrane domain of D- AKAP1a were important for targeting. Taken together, D- AKAP1a can be classified as a signal-anchored protein in the MOM. Our topological study provides valuable information about the molecular and cellular mechanisms of mitochondrial targeting of AKAP1. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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