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Preeclampsia ( PE), especially severe PE including early (before 34 weeks' gestation) and late (after 34 weeks' gestation) onset PE, is one of the leading causes of maternal and fetal mortality and morbidity. It is well known that abnormal human leukocyte antigen subtype G ( HLA-G) expression may contribute to PE. In this study, we investigated allelic and genotypic frequencies of the 14 bp deletion/insert polymorphism in the 3′-untranslated region (3′- UTR) of the HLA-G gene in cases (120 pairs of mother-offspring, 82 couples, and 67 pairs of father-offspring with severe PE) and controls (158 pairs of mother-offspring, 87 couples, and 75 pairs of father-offspring with normal pregnancy). We found that the frequencies of the +14 bp/+14 bp HLA-G genotype of the offspring were significantly higher in the severe and early onset severe PE cases compared with controls, and the frequencies of the −14 bp/−14 bp HLA-G genotype of the offspring were significantly lower in the early onset severe PE cases compared with controls. The frequency of combined −14 bp/+14 bp mother/ +14 bp/+14 bp offspring genotypes was significantly higher in the severe and early onset severe PE cases compared with controls, and the frequency of combined −14 bp/+14 bp mother/ −14 bp/−14 bp offspring genotypes was significantly lower in the early onset severe PE cases compared with late onset severe PE cases. The frequency of combined −14 bp/−14 bp father/−14 bp/−14 bp offspring genotypes was significantly lower in the early onset severe PE cases compared with late onset severe PE cases and controls. In overview, the HLA-G 14 bp deletion/insert polymorphism is associated with severe PE in father-offspring, and its distribution is different between the early and late onset severe PE. [ABSTRACT FROM AUTHOR]

The biological significance of human leukocyte antigen-G (HLA-G) is now beyond its initial concepts on the fetal–maternal immune regulation. HLA-G in various pathophysical conditions has been investigated, such as autoimmunity, tumor, inflammation and transplantation. HLA-G has also been postulated as a chemotherapy response marker both in protein and in genetic contexts. In the current study, a total of 640 Chinese Han and 258 Chinese She ethnic minority populations had been genotyped for the 14 bp insertion (+14 bp) and deletion (−14 bp) polymorphism in the HLA-G gene. Significant difference was observed for both the +14 bp/+14 bp (15.2% in the Han and 6.6% in the She; P = 0.00048, Pc = 0.00097) and −14 bp/−14 bp (34.5% in the Han and 50.4% in the She; P = 1.05 × 10−5, Pc = 2.1 × 10−5) genotypes between the two populations, and similar significance was found for both +14 bp (40.3% in the Han and 28.1% in the She) and −14 bp allele distributions ( P = 1.2 × 10−6, Pc = 2.4 × 10−6). Furthermore, frequencies of the 14 bp genotype and alleles both in the Chinese Han and in the Chinese She populations were compared with other ethnic populations. Data showed that dramatic variations between different ethnic populations were also observed for this polymorphism. In summary, our results indicate that heterogeneity of the 14 bp polymorphism in the HLA-G gene among different ethnic populations is possibly the result of evolution. [ABSTRACT FROM AUTHOR]

A novel human leukocyte antigen (HLA) class I allele, HLA-B*3713, has been identified in a Chinese individual. The HLA-B*3713 allele differs from the closest matching allele B*370101 by one nucleotide substitutions in exon 3 at nt 527(T→A), resulting in an amino acid change from Val (GTG) to Glu (GAG) at codon 152. [ABSTRACT FROM AUTHOR]

HLA-C*15:02:04 differs from HLA-C*15:02:01 by the nucleotide exchange at position 143. [ABSTRACT FROM AUTHOR]

HLA-A*02:145 differs from HLA-A*02:02:01:01 by the amino acid exchange at position 153 Ala changes to Val. [ABSTRACT FROM AUTHOR]

HLA-DQB1*06:01:04 differs from HLA-DQB1*06:01:01 by the nucleotide exchange at position 156 (G >C) without an amino exchange. [ABSTRACT FROM AUTHOR]

HLA-A*02:129 differs from HLA-A*02:87 exon 2 at nt 25, nt 29, nt 167 T, nt 184 and nt 192. [ABSTRACT FROM AUTHOR]

The novel HLA-B*39:46 differs from HLA-B*39:01:01:01 by one base substitution at position 27(C>G) of exon 4. [ABSTRACT FROM AUTHOR]

The sequence of a novel allele, HLA-B*40:124:02, differs from HLA-B*40:42 by three-nucleotide exchanges in exon 3. [ABSTRACT FROM AUTHOR]

The sequence of HLA-B*40:01:06 differs in exon 3 from the sequence of B*40:01:01 by one nucleotide 435 (codon121) G to A. [ABSTRACT FROM AUTHOR]

The sequence of HLA-B*27:44 differs in exon 2 from the sequence of B*27:08 by one amino acid change at codon 74. [ABSTRACT FROM AUTHOR]

A novel HLA-C allele, C*07:56:01, is the close matching allele C*07:02:01:01 by one nucleotide substitution in exon 2, at position 343 G>A. [ABSTRACT FROM AUTHOR]

Two new HLA-A alleles, A*23:20 and A*02:127, differ from their closely related alleles by nucleotide mutations. [ABSTRACT FROM AUTHOR]

The novel allele differs from HLA- B*07:02:01 by a single nucleotide substitution at position 538 in exon 3. [ABSTRACT FROM AUTHOR]

The novel HLA-A*24:128 shows one nucleotide change from HLA-A*24:02:01:01 in exon 2 at position 155 from A to G. [ABSTRACT FROM AUTHOR]

The A*24:02:17 allele differs from the most close matching allele A*24:02:01:01 by one nucleotide substitution in exon 2, at position 264. [ABSTRACT FROM AUTHOR]

A novel human leukocyte antigen (HLA)-DRB1 allele, DRB1*08:36, has been identified during routine sequence-specific oligonucleotide (SSO) typing and sequence-based typing (SBT) in a sample from a registered donor of Chinese Marrow Donor Program. The DRB1*08:36 allele differs from the closest related DRB1*08:03:02 allele by one nucleotide substitution in exon 2, at position 181 which results in an amino acid replacement at codon 32. Tyr is exchanged for His in the novel allele. These findings add a novel member to the HLA-DRB1*08 family. [ABSTRACT FROM AUTHOR]

A novel human leukocyte antigen-A allele, A*2489, was identified in a Chinese registered donor. The A*2489 allele differs from the closest matching allele A*2428 by two nucleotide substitutions, 257(A→G) and 265(G→C), in exon 2. [ABSTRACT FROM AUTHOR]

We describe the identification of a new DRB1*14 allele, DRB1*1461, found in a Chinese individual. The novel allele has been identified in routine polymerase chain reaction-sequence-specific oligonucleotide and sequence-based typing. The nucleotide sequence of DRB1*1461 is identical to DRB1*1404 except for a single substitution in codon 16 (TAT→CAT), leading to a change from Tyr to His. [ABSTRACT FROM AUTHOR]

A novel human leukocyte antigen-A allele, A*240212, has been identified during routine sequence-specific oligonucleotide typing and sequence-based typing in a sample from a registered donor of Chinese Marrow Donor Program. The A*240212 allele differs from the most closely matching allele A*24020101 by one nucleotide substitution in Exon 2, at position 230, while the nucleotide exchange is silent mutation. [ABSTRACT FROM AUTHOR]

The novel allele human leukocyte antigen (HLA)-A*11:01: 10 differs from HLA-A*11:01:10 by a synonymous nucleotide exchange at codon 146 in exon 2 (G/A). Here, we describe the identification of the novel allele HLA-A*11:01:10, which has been detected in a registered donor of the China Marrow Donor Program. The complete HLA typing results were as follows: A*02:01, *11:01:10; B*15:11, *15:18; C*03:03, *08:01; DRB1*08:02, *15:01. [ABSTRACT FROM AUTHOR]