1. Molecular cloning and sequencing of salmon gonadotropin β subunit.
- Author
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Trinh, Khiet-Yen, Wang, Nam C., Hew, Choy L., and Crim, Laurence W.
- Subjects
- *
GONADOTROPIN , *MOLECULAR cloning , *CHINOOK salmon , *HIGH performance liquid chromatography , *TESTOSTERONE , *PITUITARY hormones , *MESSENGER RNA - Abstract
Reports on a study in which gonadotropin (GTH) was purified from the pituitaries of the Pacific chinook salmon using a combination of stepwise ethanol precipitation and concanavalin-A affinity chromatography. Reverse-phase high-performance liquid chromatography; Screening of an enriched cDNA library for the β-GTH genes using two synthetic oligonucleotides; Effect of testosterone implantation on pituitary PTH and β-GTH mRNA; Corresponding increase in both the pituitary GTH and mRNA levels.
- Published
- 1986
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