1. Dynamic subcellular localization of aquaporin-7 in white adipocytes.
- Author
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Miyauchi, Takayuki, Yamamoto, Hiroyuki, Abe, Yoichiro, Yoshida, Go J., Rojek, Aleksandra, Sohara, Eisei, Uchida, Shinichi, Nielsen, Søren, and Yasui, Masato
- Subjects
AQUAPORINS ,FAT cells ,ADIPOSE tissues ,GLYCERIN ,LIPID metabolism ,CELL membranes ,HYPERTROPHY - Abstract
Aquaporin-7 (AQP7) is expressed in adipose tissue, permeated by water and glycerol, and is involved in lipid metabolism. AQP7-null mice develop obesity, insulin resistance, and adipocyte hypertrophy. Here, we show that AQP7 is expressed in adipocyte plasma membranes, and is re-localized to intracellular membranes in response to catecholamine in mouse white adipose tissue. We found that internalization of AQP7 was induced by PKA activation and comparative gene identification 58 (CGI-58). This relocation was confirmed by functional studies in 3T3-L1 adipocytes. Collectively, these results suggest that AQP7 makes several contributions to adipocyte metabolism, in both cortical and intracellular membranes. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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