1. Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon
- Author
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Pagmantidis, Vasileios, Bermano, Giovanna, Villette, Stephane, Broom, Iain, Arthur, John, and Hesketh, John
- Subjects
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GLUTATHIONE , *PEROXIDASE , *GENE expression , *MESSENGER RNA - Abstract
Abstract: Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60–83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17–25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletion. [Copyright &y& Elsevier]
- Published
- 2005
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