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7 results on '"Zolfo M"'

1. Successful treatment of PCP episodes caused by Pneumocystis carinii with mutant dihydropteroate (DHPS) gene.

Author

Visconti E, Ortona E, Margutti P, Marinaci S, Zolfo M, Celentano LP, Mencarini P, Siracusano A, and Tamburrini E

Subjects
AIDS-Related Opportunistic Infections microbiology, Antifungal Agents therapeutic use, Drug Therapy, Combination, Genes, Fungal, Humans, Mutation, Mycological Typing Techniques, Pneumocystis enzymology, Pneumonia, Pneumocystis microbiology, AIDS-Related Opportunistic Infections drug therapy, Dihydropteroate Synthase genetics, Pneumocystis genetics, Pneumonia, Pneumocystis drug therapy
Published
1999

2. Identification of dihydropteroate (DHPS) gene mutant in Pneumocystis carinii in respiratory samples of HIV+ patients from 1992 to 1997.

Author

Visconti E, Ortona E, Margutti P, Marinaci S, Zolfo M, Mencarini P, Celentano LP, Siracusano A, and Tamburrini E

Subjects
DNA, Fungal analysis, DNA, Fungal genetics, Dihydropteroate Synthase metabolism, Genes, Fungal, Humans, Mutation, Pneumocystis genetics, Polymerase Chain Reaction, Sputum microbiology, AIDS-Related Opportunistic Infections microbiology, Bronchoalveolar Lavage Fluid microbiology, Dihydropteroate Synthase genetics, Pneumocystis enzymology, Pneumonia, Pneumocystis microbiology
Published
1999

3. Typing with ITS regions of P.carinii from AIDS patients with recurrent pneumonia.

Author

Margutti P, Visconti E, Mencarini P, Zolfo M, Marinaci S, Tamburrini E, Siracusano A, and Ortona E

Subjects
Bronchoalveolar Lavage Fluid microbiology, Genetic Variation genetics, Humans, Recurrence, Sequence Analysis, DNA, AIDS-Related Opportunistic Infections microbiology, DNA, Fungal genetics, DNA, Ribosomal genetics, Mycological Typing Techniques, Pneumocystis genetics, Pneumonia, Pneumocystis microbiology
Abstract

To understand the way of reinfection of Pneumocystis carinii we have analyzed the genetic variation at the internal transcribed spacer (ITS) in DNA samples from bronchoalveolar lavage fluid of Italian HIV patients who had multiple episodes of P.carinii pneumonia. The presence of the same and/or a new type in both episodes suggest the possible occurrence of both reactivation of a previously acquired infection and reinfection from an exogenous source. Furthermore the occurrence of two different types in the same episode indicate that a mixed infection is common.

Published
1997
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4. Detection of Pneumocystis carinii in oropharyngeal washings by PCR-SHELA and nested PCR.

Author

Tamburrini E, Ortona E, Visconti E, Margutti P, Mencarini P, Zolfo M, Marinaci S, and Siracusano A

Subjects
AIDS-Related Opportunistic Infections microbiology, Fluorescent Antibody Technique, Indirect, Humans, Molecular Probe Techniques, Nasal Lavage Fluid microbiology, Pneumonia, Pneumocystis microbiology, Sensitivity and Specificity, AIDS-Related Opportunistic Infections diagnosis, Oropharynx microbiology, Pneumocystis isolation & purification, Pneumonia, Pneumocystis diagnosis, Polymerase Chain Reaction methods
Abstract

Oropharyngeal washings (Ophs) from 27 HIV infected patients (18 with P. carinii pneumonia, PCP, and 9 without PCP) were examined for P. carinii using morphological staining and DNA amplification with PCR-SHELA and nested PCR methods. The comparison of these techniques shows that 1. the amplification of P. carinii DNA is more sensitive than (and as specific as) morphological staining; 2. PCR-SHELA is less sensitive than (and as specific as) nested PCR.

Published
1997
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5. Cellular and humoral response in Pneumocystis carinii spontaneously infected rabbits.

Author

Ortona E, Visconti E, Barca S, Margutti P, Mencarini P, Zolfo M, Tamburrini E, and Siracusano A

Subjects
Animals, Bronchoalveolar Lavage Fluid immunology, Bronchoalveolar Lavage Fluid microbiology, Disease Models, Animal, Epitopes analysis, Female, Immunity, Cellular, Immunoglobulin G blood, Immunoglobulin M blood, Lung enzymology, Lung microbiology, Macrophages, Alveolar immunology, Nitric Oxide Synthase metabolism, Pneumocystis isolation & purification, Pneumonia, Pneumocystis microbiology, Rabbits, Antibodies, Fungal blood, Pneumocystis immunology, Pneumonia, Pneumocystis immunology
Published
1997
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6. Detection of Pneumocystis carinii DNA in HIV patients with P. carinii pneumonia (PCP) and in animal models.

Author

Tamburrini E, Mencarini P, Visconti E, Zolfo M, De Luca A, Siracusano A, Ortona E, Margutti P, Wakefield AE, Peters SE, Denis CM, and Dei-Cas E

Subjects
Animals, Disease Models, Animal, Female, Humans, Immunosuppressive Agents pharmacology, Pneumocystis genetics, Rabbits, Steroids pharmacology, AIDS-Related Opportunistic Infections microbiology, DNA, Fungal analysis, Pneumocystis isolation & purification, Pneumonia, Pneumocystis microbiology
Published
1996
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7. Comparison of two PCR methods for detection of Pneumocystis carinii in bronchoalveolar lavage fluid.

Author

Tamburrini E, Mencarini P, Visconti E, Zolfo M, De Luca A, Siracusano A, Ortona E, Margutti P, and Wakefield AE

Subjects
DNA, Fungal analysis, DNA, Ribosomal, Humans, Pneumocystis genetics, RNA, RNA, Fungal analysis, RNA, Mitochondrial, Sensitivity and Specificity, AIDS-Related Opportunistic Infections microbiology, Bronchoalveolar Lavage Fluid microbiology, Pneumocystis isolation & purification, Pneumonia, Pneumocystis microbiology, Polymerase Chain Reaction methods
Published
1996
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