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AIM This study aimed to investigate the correlation between the expression of A-kinase anchor protein95 (AKAP95), p-retinoblastoma (phosphorylated Rb, p-Rb), cyclin D2, cyclin D3 and cyclin E2 in esophageal cancer tissues and clinicopathological indexes. METHOD The protein expression levels of AKAP95, p-Rb, cyclin D2/3 and cyclin E2 in 40 esophageal cancer tissues were detected using immunohistochemistry, and the correlation between them was analyzed. RESULT The percentage of p-Rb (Ser780)-, cyclin D2-, cyclin D3- and cyclin E2-positive samples was 62.50%, 70.00%, 67.50% and 60.00%, respectively. Also, the positive expression did not correlate with the histological type, histological differentiation or lymph node metastasis. The expression of AKAP95 and p-Rb (Ser780), p-Rb (Ser780) and cyclin D2 and p-Rb (Ser780) and cyclin D3 in esophageal cancer tissues was found to be correlated (P

Horseradish peroxidase (HRP)-mediated crosslinking of poly(amido amine) (PAA) copolymers was successfully applied in the preparation of in situ forming degradable hydrogels under physiological conditions. PAA copolymers containing different amounts of tyramine residues (termed as pAEEOL/TA) could be synthesized through Michael-type addition between methylenebisacryamide and amine mixture of 2-(2-aminoethoxy) ethanol and tyramine (TA). Depending on the amount of TA residue, the HRP, and H2O2 concentration, the gelation times could be varied from about 50 to 350 s. The swelling and degradation experiments indicated under physiological conditions the pAEEOL/TA-based hydrogels are completely degradable within 6–8 days. Rheological analysis revealed that storage modulus of the hydrogels increased from 2500 to 4100 Pa when increasing HRP concentrations. Importantly, pAEEOL/TA copolymers have low cytotoxicity. Moreover, NIH 3T3 (mouse embryonic fibroblast) cells exposed in the degradation products of pAEEOL/TA-based hydrogels retained high cell viability, implying that the hydrogels are cyto-biocompatible. In vitro release of methylene blue and IgG protein from pAEEOL/TA-based hydrogels could be effectively sustained by encapsulation of the drug in the hydrogels. The results indicate that HRP-crosslinked, degradable pAEEOL/TA-based hydrogels are promising for biomedical applications. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

A newly developed hydrogen peroxide selective membrane electrode using lettuce seed meal as source biocatalyst is described. This amperometric-based probe exhibits high biocatalytic activity, long lifetime and favorable selectivity for the measurement of hydrogen peroxide.

The rough pyramidal gold nanostructures fabricated by a one-step, nontemplated, low-cost electrochemical deposition, combined with a perfluorodecanethiol coating and, more importantly, the very large fraction of air trapped into the gold film, create a super-amphiphobic surface.