1. Pioglitazone attenuates advanced glycation end products‐induced apoptosis and calcification by modulating autophagy in tendon‐derived stem cells
- Author
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Yan Xiong, Kai Xu, Zhipeng Wu, Caihua Zhang, Yuzhe He, Lidong Wu, Jisheng Ran, Zhonggai Chen, Safwat Adel Abdo Moqbel, Langhai Xu, and Chiyuan Ma
- Subjects
Glycation End Products, Advanced ,Male ,0301 basic medicine ,autophagy ,Receptor for Advanced Glycation End Products ,Rats, Sprague-Dawley ,Tendons ,03 medical and health sciences ,Calcification, Physiologic ,0302 clinical medicine ,Glycation ,medicine ,Animals ,Receptor ,Pioglitazone ,business.industry ,advanced glycation end products ,Stem Cells ,Autophagy ,apoptosis ,Original Articles ,Cell Biology ,medicine.disease ,Rats ,PPAR gamma ,030104 developmental biology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Original Article ,Stem cell ,Tendinopathy ,business ,tendon‐derived stem cells ,medicine.drug ,Calcification - Abstract
Diabetes mellitus (DM) is one of the prominent risk factors for pathological development and progression of tendinopathy. One feature of DM‐related changes in tendinopathy is accumulation of advanced glycation end products (AGEs) in affected tendons. Pioglitazone (Pio), a peroxisome proliferator‐activated receptor γ agonist, performs a protective effect against AGEs. The present study aimed to investigate the pathogenetic role of AGEs on tendon‐derived stem cells (TDSCs) and to determine the effect of Pio on AGEs‐induced TDSC dysfunctions. Results indicated that AGEs induced TDSC apoptosis as well as compensatory activation of autophagy. Pharmacologic activation/inhibition of autophagy leaded to alleviate/exacerbate apoptosis induced by AGEs. We further confirmed the effect of Pio on autophagy, which ameliorated apoptosis and abnormal calcification caused by AGEs both in vitro and in vivo. Thus, we suggest that Pio ameliorates the dysfunctions of TDSCs against AGEs by promoting autophagy, and we also reveal that Pio is a potential pharmacological choice for tendinopathy.
- Published
- 2020
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