1. Evidence for additionally increased apoptosis in the peripheral blood mononuclear cells of major depressive patients with a high risk for suicide
- Author
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Giselli Scaini, Meysam Amidfar, João Quevedo, and Yong Ku Kim
- Subjects
Adult ,Male ,medicine.medical_specialty ,Apoptosis ,Suicide, Attempted ,Peripheral blood mononuclear cell ,Blood cell ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Immune system ,Risk Factors ,Internal medicine ,Humans ,Medicine ,RNA, Messenger ,fas Receptor ,Gene ,Genetics (clinical) ,bcl-2-Associated X Protein ,Depressive Disorder, Major ,Depression ,business.industry ,Gene Expression Profiling ,medicine.disease ,Pathophysiology ,030227 psychiatry ,Suicide ,Psychiatry and Mental health ,medicine.anatomical_structure ,Endocrinology ,Real-time polymerase chain reaction ,Proto-Oncogene Proteins c-bcl-2 ,Leukocytes, Mononuclear ,Major depressive disorder ,Female ,business ,030217 neurology & neurosurgery - Abstract
Several studies have suggested a pathophysiological role of blood cell apoptosis in major depressive disorder (MDD). The aim of this study was to evaluate mRNA expression levels of Bcl-2, Bax, and Fas in peripheral blood mononuclear cells (PBMCs) of MDD patients with a high risk for suicide relative to those without a high risk for suicide as well as healthy subjects. The mRNA expression of Bcl-2, Bax, and Fas as well as the Bcl-2/Bax ratio was examined in the PBMCs of 30 MDD patients with a high risk for suicide, 30 MDD patients without a high risk for suicide, and 30 healthy controls. The mRNA expression of target genes was measured using real-time quantitative Polymerase Chain Reaction (PCR). FAS mRNA expression was significantly increased, and Bcl-2 mRNA expression and the Bcl-2/Bax expression ratio were significantly decreased, in the PBMCs of MDD patients with or without a high risk for suicide attempts compared to healthy controls (p
- Published
- 2018