12 results on '"Yingfeng Du"'
Search Results
2. Characterization of Fritillariae cirrhosae bulbus from multiple sources by potential Q‐marker based on metabolomics and network pharmacology
- Author
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Xi Tian, Jiali Hou, Mengxin Yang, Miaoting Zhang, Wenjing Sun, Shuai Guan, Yingfeng Du, and Yiran Jin
- Subjects
Fritillaria ,Organic Chemistry ,Metabolomics ,Network Pharmacology ,Medicine, Chinese Traditional ,Spectroscopy ,Drugs, Chinese Herbal ,Analytical Chemistry - Abstract
Fritillaria cirrhosae bulbus (BFC), a typical traditional Chinese medicine with multiple botanical sources, has been used for relieving cough and reducing sputum. Studies have shown that there were obvious differences in the chemical compositions and clinical efficacy of different sources of BFC. How to characterise BFC from botanical sources accurately and quickly is vital for drug quality evaluation and clinical applications.In the present study, an integrated strategy of plant metabolomics combined with the target network pharmacology was developed to characterise BFC. Plant metabolomics analysis was performed to screen out the chemical markers of six species of BFC. Then, target network pharmacology was applied to explore the relationship between chemical markers and related diseases. Finally, potential Q-markers for species characterization were selected by combined analysis of plant metabolomics and the target network pharmacology.A total of 67 Fritillaria alkaloid compounds were identified. Six species showed clear characterization by multivariate statistical analysis, resulting in 12 chemical markers. Meanwhile, a total of nine components related to asthma were screened out based on the target network pharmacology. Taking content difference and pharmacological activity into consideration, nine constituents were selected as potential Q-markers.The method developed provided not only a standard protocol for characterising different species of BFC directly, but also an effective approach for multisource medicines discrimination.
- Published
- 2022
3. Characterisation of hederacoside C metabolites using ultrahigh‐performance liquid chromatography quadrupole Orbitrap mass spectrometry based on automatic fragment ion search
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Cheng Li, Huijun Xu, Qiao Wang, Qian Sun, Wendan Zhang, Jianxi Yang, Honghong Jiang, and Yingfeng Du
- Subjects
Plant Science ,Orbitrap ,Mass spectrometry ,01 natural sciences ,Biochemistry ,Hederacoside C ,Mass Spectrometry ,Analytical Chemistry ,law.invention ,Triterpenoid ,Triterpene ,In vivo ,law ,Drug Discovery ,Animals ,Oleanolic Acid ,Spectral data ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Chromatography ,Chemistry ,010401 analytical chemistry ,Orbitrap ms ,General Medicine ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,Molecular Medicine ,Chromatography, Liquid ,Food Science - Abstract
Introduction Hederacoside C (HDC) is a bioactive natural triterpenoid saponins constituent originating from traditional Chinese medicines, playing an important role in the treatment of acute respiratory infections and chronic inflammatory bronchitis. Meanwhile, it is recognised by Korea as a botanical drug. Objectives In order to develop an integrated template approach to analysing screening and identification of the metabolites of traditional Chinese medicines. This study will provide available information for further pharmaceutical studies of HDC and other triterpene saponins. Methodology An analysis strategy based on ultrahigh-performance liquid chromatography quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS) technique combined with automatic fragment ion search (FISh) was firstly exploited for the characterisation metabolites of HDC in vivo and in vitro. Accurate full mass scan combined with an on-line FISh annotations approach was developed to rapidly identify all the potential metabolites of HDC. Furthermore, FISh accurately located the structure of the target compound in a large number of mass spectral data. Results A total of 34 metabolites were detected and tentatively identified by analysing comprehensive biological samples. The results clearly demonstrated that HDC underwent general metabolic reactions including dealkylation, reduction, oxidation, desaturation, dehydration, cysteine conjugation, GSH conjugation, taurine conjugation, and glycine conjugation to produce 26 phase I and eight phase II metabolites. Conclusion In the present study, UHPLC-Q-Exactive Orbitrap MS technique combined with FISh provided a rapid and efficient platform to characterise metabolites of HDC in vivo and in vitro. The proposed method could develop an integrated template approach to screen and identify the constituents and metabolites of traditional Chinese medicines.
- Published
- 2020
4. Simultaneous determination of 12 active components in the roots ofPulsatilla chinensisusing tissue-smashing extraction with liquid chromatography and mass spectrometry
- Author
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Wendan Zhang, Huijun Xu, Yan-mei Xu, Liang Cao, Miaomiao Jin, Qian Sun, Yingfeng Du, and Wei Guo
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Chromatography ,010405 organic chemistry ,Chemistry ,Negative mode ,010401 analytical chemistry ,Analytical chemistry ,Active components ,Filtration and Separation ,Mass spectrometry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Principal component analysis ,Ultra high performance ,Quantitative analysis (chemistry) - Abstract
Ultra high performance liquid chromatography coupled with mass spectrometry and combining a tissue-smashing extraction technique was developed for the simultaneous quantitative analysis of 12 compounds in the roots of Pulsatilla chinensis. Among them, compound 6 was characterized and accurately quantified in this herb for the first time. The parameters of extraction condition were simultaneously optimized with a Box-Behnken design and Derringer's function. The optimized conditions were as follows: sample quantity of 0.5 g, ethanol concentration of 70%, and extraction time of 200 s. Multiple-reaction monitoring scanning was employed for the quantification between positive and negative mode in a single run of 6 min. Full validation of the method was carried out, and the results indicated that the method was rapid, specific, and reliable. The developed method was successfully applied to quantify the 12 compounds in 33 batches of P. chinensis from different provinces. Moreover, the principal component analysis was performed to compare the P. chinensis collected from different provinces of China based on quantitative data and the results indicated that the content of compounds could be used to differentiate the origins of P. chinensis. These results demonstrated that this method is feasible and reliable for the quality control of P. chinensis.
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- 2017
5. Simultaneous analysis of 11 main active components in Cirsium setosum based on HPLC-ESI-MS/MS and combined with statistical methods
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Lu Chang, Qiao Wang, Yanping Ren, Yingfeng Du, Xiaowei Shi, Yingguang Sun, Liang Cao, Qian Sun, and Lantong Zhang
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Detection limit ,Analyte ,Electrospray ,Chromatography ,Chemistry ,Electrospray ionization ,Analytical chemistry ,Filtration and Separation ,Quadrupole ion trap ,Tandem mass spectrometry ,Mass spectrometry ,Ion source ,Analytical Chemistry - Abstract
A novel method based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed for simultaneous determination of the 11 major active components including ten flavonoids and one phenolic acid in Cirsium setosum. Separation was performed on a reversed-phase C(18) column with gradient elution of methanol and 0.1‰ acetic acid (v/v). The identification and quantification of the analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. Multiple-reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Full validation of the assay was carried out including linearity, precision, accuracy, stability, limits of detection and quantification. The results demonstrated that the method developed was reliable, rapid, and specific. The 25 batches of C. setosum samples from different sources were first determined using the developed method and the total contents of 11 analytes ranged from 1717.460 to 23028.258 μg/g. Among them, the content of linarin was highest, and its mean value was 7340.967 μg/g. Principal component analysis and hierarchical clustering analysis were performed to differentiate and classify the samples, which is helpful for comprehensive evaluation of the quality of C. setosum.
- Published
- 2012
6. Simultaneous determination of nine components in Anemarrhena asphodeloides by liquid chromatography-tandem mass spectrometry combined with chemometric techniques
- Author
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Yanping Ren, Yingfeng Du, Liang Cao, Xiaowei Shi, Lu Chang, Yingguang Sun, Qiao Wang, Yong Liu, Lantong Zhang, and Qian Sun
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Electrospray ,Chromatography ,biology ,Chemistry ,Electrospray ionization ,Selected reaction monitoring ,Analytical chemistry ,Filtration and Separation ,Tandem mass spectrometry ,Mass spectrometry ,biology.organism_classification ,Ion source ,Analytical Chemistry ,Anemarrhena asphodeloides ,Liquid chromatography–mass spectrometry - Abstract
A novel quantitative method using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry was developed for simultaneous determination of the important active constituents including four steroidal saponins, two xanthone glycosides, two isoflavonoids, and one anthraquinone in different parts of Anemarrhena asphodeloides from different habitats. Hierarchical clustering analysis and principal components analysis were performed to differentiate and classify the samples. The separation was performed on a C(18) column with acidified aqueous acetonitrile gradients. Quantification of the analytes was achieved by use of a hybrid quadrupole linear ion-trap mass spectrometer. Multiple-reaction monitoring scanning was employed with switching electrospray ion source polarity between positive and negative modes in a single run. The validation results of the method indicated that the method was simple, rapid, specific, and reliable. The results demonstrated that the quantitative difference in content of nine active compounds was useful not only for chemotaxonomy of many samples from different sources but also for the standardization and differentiation of many similar samples. Simultaneous quantification of bioactive components by HPLC-ESI-MS coupled with chemometric techniques would be a well-acceptable strategy to comprehensively control the quality of A. asphodeloides.
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- 2012
7. Quality Evaluation of a Herbal Prescription Through Quantification of 40 Components by HPLC-ESI-MS/MS
- Author
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Man Liu, Xiaowei Shi, Yingfeng Du’ Lantong Zhang, Songchen Liu, Minyan Liu, and Qiao Wang
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Detection limit ,Analyte ,Chromatography ,Negative mode ,Chemistry ,Formic acid ,Hplc esi ms ms ,Plant Science ,General Medicine ,Mass spectrometry ,Biochemistry ,Analytical Chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Drug Discovery ,Molecular Medicine ,Gradient elution ,Quadrupole ion trap ,Food Science - Abstract
Introduction The Kang-nao-shuai (KNS) capsule is a combined herbal prescription used in the treatment of insomnia, amnesia, neurasthenia, age-related dementia and brain injuries. Multiple constituents are considered to be responsible for the therapeutic effects of this herbal prescription. However, the quality control of the multicomponents is limited. Objective To establish a liquid chromatography–electrospray ionisation–mass spectrometry method for the analysis of 40 constituents in KNS capsules. Methodology The optimal chromatographic conditions were achieved on an Agilent C18-column with a gradient elution that consisted of methanol and 0.1% formic acid in water. The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer in positive and negative mode respectively using multiple-reaction monitoring. Results A total of 40 constituents including organic acid, flavonoid, quinone, terpene, alkaloid and saponin were quantified, most of the 40 components were determined for the first time in the KNS capsule. A quantitative HPLC–ESI–MS/MS method allowing the quantification of 40 marker compounds was optimised and validated for linearity, precision, accuracy, stability, specificity and limits of detection and quantification. The method was successfully applied to analyse 10 batches of KNS capsule. Conclusion The established method is simple and can be used as a tool for quality evaluation and control of this natural product. Copyright © 2011 John Wiley & Sons, Ltd.
- Published
- 2011
8. Tentative identification of new metabolites of epimedin C by liquid chromatography-mass spectrometry
- Author
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Xiaowei Shi, Wang Zongquan, Hairong Wang, Lantong Zhang, Hongtao Wang, Honghui Xu, Minyan Liu, Shaohua Zhao, Zi-Ming Lu, and Yingfeng Du
- Subjects
Chromatography ,Metabolite ,Filtration and Separation ,Glucuronic acid ,Analytical Chemistry ,Hydroxylation ,chemistry.chemical_compound ,Metabolic pathway ,Hydrolysis ,chemistry ,Liquid chromatography–mass spectrometry ,Organic chemistry ,Ion trap ,Demethylation - Abstract
Epimedin C is one of the major bioactive constituents of Herba Epimedii. The aim of this study is to characterize and elucidate the structure of metabolites in the rat after administration of epimedin C. Metabolite identification was performed using a predictive multiple reaction monitoring–information dependent acquisition–enhanced product ion (pMRM-IDA-EPI) scan in positive ion mode on a hybrid triple quadrupole-linear ion trap mass spectrometer. A total of 18 metabolites were characterized by the changes in their protonated molecular masses, their MS/MS spectrum and their retention times compared with those of the parent drug. The results reveal possible metabolite profiles of epimedin C in rats; the metabolic pathways including hydrolysis, hydroxylation, dehydrogenation, demethylation and conjugation with glucuronic acid and different sugars were observed. This study provides a practical approach for rapidly identifying complicated metabolites, a methodology that could be widely applied for the structural characterization of metabolites of other compounds.
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- 2011
9. Rapid method for simultaneous determination of 20 components in Isodon nervosa by high-performance liquid chromatography-electrospray ionisation tandem mass spectrometry
- Author
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Lantong Zhang, Xiaowei Shi, Pengwei Liu, Yingfeng Du, Qiao Wang, Yiran Jin, Xiaona Sheng, and Xiaowei Zhang
- Subjects
Detection limit ,Electrospray ,Chromatography ,biology ,Elution ,Chemistry ,Formic acid ,Isodon ,Analytical chemistry ,Plant Science ,General Medicine ,biology.organism_classification ,Tandem mass spectrometry ,Mass spectrometry ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,Drug Discovery ,Molecular Medicine ,Food Science - Abstract
Introduction – Isodon nervosa is a commonly used traditional Chinese medicine including diterpenoids, phenolic acids, triterpenoids and volatile oil. Qualitative and quantitative analysis of multi-components is important for its quality control. Objective – To establish a liquid chromatography–electrospray ionisation–mass spectrometry method for simultaneous analysis of 20 bioactive constituents of Isodon nervosa in different places of China and different parts of this herb. Methodology – The optimal chromatographic conditions were achieved on a C18 column (250 × 4.6 mm, 5 µm) with with linear gradient elution with 0.1% aqueous formic acid : methanol containing 0.1% formic acid at a flow-rate of 0.7 mL/min in 15 min. The identification and quantification of those analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. Multiple-reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Full validation of the method was carried out (linearity, precision, accuracy, limit of detection and limit of quantification). Results – The results indicated that the method was simple, rapid, specific and reliable. The proposed method was successfully applied for the qualitative and quantitative analysis of 20 chemical compositions in Isodon nervosa samples. Conclusion – Twenty chemical compositions in 21 batches of wild and cultivated Isodon nervosa samples from different sources had great variation in the contents. Copyright © 2010 John Wiley & Sons, Ltd.
- Published
- 2010
10. Simultaneous qualitative and quantitative analysis of 28 components in Isodon rubescens by HPLC-ESI-MS/MS
- Author
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Lantong Zhang, Yingfeng Du, Xiaowei Shi, Yiran Jin, Xiaona Sheng, Zhifang Yuan, Qiao Wang, Xiaowei Zhang, and Pengwei Liu
- Subjects
Electrospray ,Chromatography ,Chemistry ,Formic acid ,Elution ,Analytical chemistry ,Filtration and Separation ,Tandem mass spectrometry ,Mass spectrometry ,High-performance liquid chromatography ,Analytical Chemistry ,chemistry.chemical_compound ,Liquid chromatography–mass spectrometry ,Quadrupole ion trap - Abstract
A novel method, HPLC-MS/MS was developed to qualitatively identify and quantitatively determine the 28 components including 19 diterpenoids, 6 phenolic acids and 3 flavonoids in Isodon rubescens, an important traditional Chinese medicine. The separation was performed on a C(18) column with linear gradient elution with 0.1% aqueous formic acid/methanol containing 0.1% formic acid at a flow rate of 0.7 mL/min. The identification and quantification of those analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. Multiple-reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Full validation of the method was carried out (linearity, precision, accuracy, LOD and LOQ). The results indicated that the method was simple, rapid, specific and reliable. The proposed method was successfully applied for the qualitative and quantitative analysis of 28 chemical compositions in 21 batches of natural and cultured I. rubescens samples from different sources which had great variation on the contents. The results demonstrated that the method was useful for standardization and differentiation of large numbers of similar samples.
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- 2010
11. Quality Evaluation of a Herbal Prescription Through Quantification of 40 Components by HPLC–ESI–MS/MS
- Author
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Liu, Man, primary, Liu, Songchen, additional, Shi, Xiaowei, additional, Liu, Minyan, additional, Zhang, Yingfeng Du’ Lantong, additional, and Wang, Qiao, additional
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- 2011
- Full Text
- View/download PDF
12. Quality evaluation of a herbal prescription through quantification of 40 components by HPLC-ESI-MS/MS.
- Author
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Liu M, Liu S, Shi X, Liu M, Zhang YD, and Wang Q
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- Benzoquinones analysis, Benzoquinones chemistry, Biomarkers analysis, Biomarkers chemistry, Chromatography, High Pressure Liquid instrumentation, Chromatography, High Pressure Liquid methods, Drug Stability, Drugs, Chinese Herbal chemistry, Flavonoids analysis, Flavonoids chemistry, Limit of Detection, Linear Models, Methanol chemistry, Molecular Structure, Plant Preparations chemistry, Quality Control, Saponins analysis, Saponins chemistry, Spectrometry, Mass, Electrospray Ionization instrumentation, Terpenes analysis, Terpenes chemistry, Time Factors, Drugs, Chinese Herbal analysis, Magnoliopsida chemistry, Plant Preparations analysis, Spectrometry, Mass, Electrospray Ionization methods
- Abstract
Introduction: The Kang-nao-shuai (KNS) capsule is a combined herbal prescription used in the treatment of insomnia, amnesia, neurasthenia, age-related dementia and brain injuries. Multiple constituents are considered to be responsible for the therapeutic effects of this herbal prescription. However, the quality control of the multicomponents is limited., Objective: To establish a liquid chromatography-electrospray ionisation-mass spectrometry method for the analysis of 40 constituents in KNS capsules., Methodology: The optimal chromatographic conditions were achieved on an Agilent C₁₈-column with a gradient elution that consisted of methanol and 0.1% formic acid in water. The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer in positive and negative mode respectively using multiple-reaction monitoring., Results: A total of 40 constituents including organic acid, flavonoid, quinone, terpene, alkaloid and saponin were quantified, most of the 40 components were determined for the first time in the KNS capsule. A quantitative HPLC-ESI-MS/MS method allowing the quantification of 40 marker compounds was optimised and validated for linearity, precision, accuracy, stability, specificity and limits of detection and quantification. The method was successfully applied to analyse 10 batches of KNS capsule., Conclusion: The established method is simple and can be used as a tool for quality evaluation and control of this natural product., (Copyright © 2011 John Wiley & Sons, Ltd.)
- Published
- 2012
- Full Text
- View/download PDF
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