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15 results on '"Yang, Xuekang"'

9. Rational Design of a Two‐Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues

Author

Fang, Haixiao, primary, Zhang, Hang, additional, Li, Lin, additional, Ni, Yun, additional, Shi, Riri, additional, Li, Zheng, additional, Yang, Xuekang, additional, Ma, Bo, additional, Zhang, Chengwu, additional, Wu, Qiong, additional, Yu, Changmin, additional, Yang, Naidi, additional, Yao, Shao Q., additional, and Huang, Wei, additional

Published
2020
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11. Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof‐of‐concept study

Author

Mengying Wei, Danna Bai, Xueying Zhou, Wenqi Sun, Guodong Yang, Yang Xuekang, Lijun Yuan, Xiaotong Gao, Zhelong Li, Yan Dong, and Lianbi Zhao

Subjects
0301 basic medicine, protein delivery, Histology, exosomes, Exosome, 03 medical and health sciences, 0302 clinical medicine, RNA interference, Lysosome, medicine, lcsh:QH573-671, lcsh:Cytology, Chemistry, RNA, Cell Biology, Transfection, Fusion protein, Microvesicles, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Membrane protein, 030220 oncology & carcinogenesis, lysosome, hur, lamp2b, Research Article
Abstract

Therapeutically intervening the function of RNA in vivo remains a big challenge. We here developed an exosome-based strategy to deliver engineered RNA-binding protein for the purpose of recruiting specific RNA to the lysosomes for degradation. As a proof-of-principle study, RNA-binding protein HuR was fused to the C-terminus of Lamp2b, a membrane protein localized in both exosome and lysosome. The fusion protein was able to be incorporated into the exosomes. Moreover, exosomes engineered with Lamp2b-HuR successfully decreased the abundance of RNA targets possibly via lysosome-mediated degradation, especially when the exosomes were acidified. The system was specifically effective in macrophages, which are lysosome enriched and resistant to routine transfection mediated RNAi strategy. In the CCl4-induced liver injury mouse model, we found that delivery of acidified exosomes engineered with Lamp2b-HuR significantly reduced liver fibrosis, together with decreased miR-155 and other inflammatory genes. In summary, the established exosome-based RNA-binding protein delivery strategy, namely “exosome-mediated lysosomal clearance”, takes the advantage of exosome in targeted delivery and holds great promise in regulating a set of genes in vivo.

Published
2020

12. Biomimetic Chiral Photonic Crystals

Author

Lv, Jiawei, primary, Ding, Defang, additional, Yang, Xuekang, additional, Hou, Ke, additional, Miao, Xiang, additional, Wang, Dawei, additional, Kou, Baichuan, additional, Huang, Ling, additional, and Tang, Zhiyong, additional

Published
2019
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14. Linagliptin inhibits high glucose-induced transdifferentiation of hypertrophic scar-derived fibroblasts to myofibroblasts via IGF/Akt/mTOR signalling pathway

Author

Li, Yan, primary, Zhang, Julei, additional, Zhou, Qin, additional, Wang, Hongtao, additional, Xie, Songtao, additional, Yang, Xuekang, additional, Ji, Peng, additional, Zhang, Wanfu, additional, He, Ting, additional, Liu, Yang, additional, Wang, Kejia, additional, Li, Xiaoqiang, additional, Shi, Jihong, additional, and Hu, Dahai, additional

Published
2018
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