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24 results on '"Yang, Hyo-In"'

3. Large population cell characterization using quantitative phase cytometer

Author

Yongjin Sung, Zahid Yaqoob, Di Jin, Yang-Hyo Kim, Niyom Lue, and Peter T. C. So

Subjects
0301 basic medicine, Histology, Resolution (mass spectrometry), Large cell, Phase (waves), Cell Biology, Biology, Bioinformatics, 01 natural sciences, Pathology and Forensic Medicine, Characterization (materials science), Sphericity, 010309 optics, 03 medical and health sciences, Interferometry, 030104 developmental biology, 0103 physical sciences, Image Cytometry, Biological system, Cytometry
Abstract

A major challenge in cellular analysis is the phenotypic characterization of large cell populations within a short period of time. Among various parameters for cell characterization, the cell dry mass is often used to describe cell size but is difficult to be measured directly with traditional techniques. Here, we propose an interferometric approach based on line-focused beam illumination for high-content precision dry mass measurements of adherent cells in a non-invasive fashion-we call it quantitative phase cytometry (QPC). Besides dry mass, abundant cellular morphological features such as projected area, sphericity, and phase skewness can be readily extracted from the QPC interferometric data. To validate the utility of our technique, we demonstrate characterizing a large population of ∼104 HeLa cells. Our reported QPC system is envisioned as a promising quantitative tool for label-free characterization of a large cell count at single cell resolution. © 2017 International Society for Advancement of Cytometry.

Published
2017
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4. Large population cell characterization using quantitative phase cytometer

Author

Jin, Di, Sung, Yongjin, Lue, Niyom, Kim, Yang-Hyo, So, Peter T. C., Yaqoob, Zahid, Lincoln Laboratory, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Mechanical Engineering, Massachusetts Institute of Technology. Laser Biomedical Research Center, Jin, Di, Sung, Yongjin, Lue, Niyom, Kim, Yang-Hyo, So, Peter T. C., and Yaqoob, Zahid

Abstract

A major challenge in cellular analysis is the phenotypic characterization of large cell populations within a short period of time. Among various parameters for cell characterization, the cell dry mass is often used to describe cell size but is difficult to be measured directly with traditional techniques. Here, we propose an interferometric approach based on line-focused beam illumination for high-content precision dry mass measurements of adherent cells in a non-invasive fashion—we call it quantitative phase cytometry (QPC). Besides dry mass, abundant cellular morphological features such as projected area, sphericity, and phase skewness can be readily extracted from the QPC interferometric data. To validate the utility of our technique, we demonstrate characterizing a large population of ∼104HeLa cells. Our reported QPC system is envisioned as a promising quantitative tool for label-free characterization of a large cell count at single cell resolution. © 2017 International Society for Advancement of Cytometry., National Institutes of Health (U.S.) (Grant 9P41EB015871-26A1), National Institutes of Health (U.S.) (Grant 1R01HL121386-01A1), Hamamatsu Corporation, Singapore-MIT Alliance for Research and Technology (SMART)

Published
2017

6. Novel sulfate tablet PBK‐1701TCversusoral sulfate solution for colon cleansing: A randomized phase 3 trial

Author

Yang, Hyo‐Joon, primary, Park, Dong Il, additional, Park, Soo‐Kyung, additional, Lee, Chang Kyun, additional, Kim, Hyo Jong, additional, Oh, Shin Ju, additional, Moon, Jung Rock, additional, Lee, Beom Jae, additional, Koh, Jin Sung, additional, Kim, Hyun Soo, additional, Park, Seon‐Young, additional, Kim, Dong Hyun, additional, Chun, Jaeyoung, additional, Kang, Eun Ae, additional, Kim, Jung, additional, Soh, Hosim, additional, Eun, Chang Soo, additional, Kim, You Sun, additional, and Jeen, Yoon Tae, additional

Published
2019
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8. Association of nasal microbiome and asthma control in patients with chronic rhinosinusitis

Author

Yang, Hyo J., primary, LoSavio, Phillip S., additional, Engen, Phillip A., additional, Naqib, Ankur, additional, Mehta, Arpita, additional, Kota, Raj, additional, Khan, Rafsa J., additional, Tobin, Mary C., additional, Green, Stefan J., additional, Schleimer, Robert P., additional, Keshavarzian, Ali, additional, Batra, Pete S., additional, and Mahdavinia, Mahboobeh, additional

Published
2018
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11. Risks of colorectal advanced neoplasia in young adults versus those of screening colonoscopy in patients aged 50 to 54 years

Author

Kim, Kyeong Ok, primary, Yang, Hyo-Joon, additional, Cha, Jae Myung, additional, Shin, Jeong Eun, additional, Kim, Hyun Gun, additional, Cho, Young-Seok, additional, Boo, Sun-Jin, additional, Lee, Jun, additional, Jung, Yunho, additional, Lee, Hyun Jung, additional, Huh, Kyu Chan, additional, Joo, Young-Eun, additional, Park, Jongha, additional, and Moon, Chang Mo, additional

Published
2017
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20. Vortex-assisted tryptic digestion

Author

Yang, Hyo-Jik, primary, Shin, Seongjae, additional, Kim, Jinhee, additional, Hong, Jangmi, additional, Lee, Sunyoung, additional, and Kim, Jeongkwon, additional

Published
2010
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