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Premise of the Study: We isolated and characterized polymorphic microsatellite loci in Cyclobalanopsis glauca (Fagaceae), an evergreen broadleaved monoecious tree, to provide tools for analyzing parentage and mating system., Methods and Results: Thirteen polymorphic microsatellite markers were developed and tested in three C. glauca populations. The number of alleles per locus varied from two to 22. The observed and expected heterozygosities within populations were 0.000-0.967 and 0.033-0.949, respectively., Conclusions: These polymorphic primers showed high levels of polymorphism within tested populations, and can be used in parentage analysis and mating system estimation of C. glauca.

Premise of the Study: Polymorphic microsatellite primers were developed in the seagrass Zostera japonica to investigate genetic variation and to identify clonal structure. •, Methods and Results: Thirteen polymorphic loci and 23 monomorphic loci were developed in Z. japonica. Two to 13 alleles per locus were observed at the polymorphic loci across 57 individuals of two Z. japonica populations. The observed and expected heterozygosities within populations ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8542, respectively. •, Conclusions: Our study showed high-level polymorphism at the polymorphic loci in Z. japonica. These primers would be a powerful tool to study genetic variation, clonal structure, and mating systems.

Premise of the Study: Polymorphic microsatellite primers were developed in the vulnerable seagrass Halophila beccarii to investigate genetic variation and provide necessary markers for studying its population genetic structure., Methods and Results: Six polymorphic and six monomorphic microsatellite loci were developed in H. beccarii. Most loci were successfully amplified across 40 H. beccarii individuals collected from three populations from coastal regions of southern China. Two to four alleles per locus were observed at the six polymorphic loci. The highest expected heterozygosity was 0.5737., Conclusions: The results demonstrate low levels of polymorphism in H. beccarii from coastal regions of southern China. They also illustrate that these primers may be useful for studying the mating system and population genetics of H. beccarii on a global scale.

Unlabelled: •, Premise of the Study: Polymorphic microsatellite primers were developed in the seagrass Halophila ovalis to investigate genetic variation. •, Methods and Results: Ten polymorphic microsatellite loci were developed in Halophila ovalis. The number of alleles per locus ranged from 2 to 12 across 80 H. ovalis individuals. These loci were successfully amplified in H. minor, and four were monomorphic across 30 individuals. •, Conclusions: These results from four H. ovalis populations and one H. minor population show the broad utility of microsatellite loci in future studies of population genetics. Four distinct alleles were present in H. minor but absent in H. ovalis, indicating potential divergence between them.