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Cryptocaryon irritans infestations on marine teleosts are a considerable burden on factory mariculture. Ultraviolet (UV) light can kill C. irritans under laboratory conditions. However, a rational method for using UV in factory aquaculture to control cryptocaryoniasis has not been developed. This study focused on evaluating the killing effect of UV on protomonts and tomonts of C. irritans and established an automatic UV parasiticide device for the prevention and control of cryptocaryoniasis in marine teleosts.The survival rate of protomonts and tomonts decreased with an increase in the UV irradiation dose. All the protomonts and tomonts died within 14 and 24 min, respectively. The lowest UV lethal doses of protomonts and tomonts of C. irritans were 2.0 × 10The automation of traditional physical methods conforms to the sustainable development of aquaculture and provides a theoretical reference for the prevention and control of cryptocaryoniasis in mariculture. © 2022 Society of Chemical Industry.

Cryptocaryonosis is the greatest threat to most teleost species among all parasitic diseases, causing mass loss to the marine aquaculture industry. Epidemiological investigation of teleost susceptibility to Cryptocaryon irritans infection revealed that yellow drum (Nibea albiflora) is highly resistant. In order to further understand the activation of the immune system in the gill, which is one of the main mucosal-associated lymphoid tissues and a target of parasites, transcriptome analysis of the yellow drum gill was performed. Gill samples were collected from fish challenged after 24 hr and 72 hr with theronts at a median death rate (2050 theronts per gram fish). Gene expression profiles showed that TLR5 was the only receptor that activated the downstream immune response. The infection activated complement cascade through alternative pathway and increased the expression of C5a anaphylatoxin chemotactic receptor 1. In addition, possible antimicrobial molecules, including lipoprotein and haptoglobin, which are responsible for trypanolysis in humans, were among the top significantly upregulated genes at 24 hr. After 72 hr, the expression of secreted immunoglobulin T-related genes was induced. These results suggested a rapid innate and adaptive immune response at the mucosal level. In conclusion, the results provide new perspectives on mucosal immune resistance in yellow drum against cryptocaryonosis and provide the possibility of mining resistance genes for future therapy.

Pie and doughnut charts nicely convey the part–whole relationship and they have become the most recognizable chart types for representing proportions in business and data statistics. Many experiments have been carried out to study human perception of the pie chart, while the corresponding aspects of the doughnut chart have seldom been tested, even though the doughnut chart and the pie chart share several similarities. In this paper, we report on a series of experiments in which we explored the effect of a few fundamental design parameters of doughnut charts, and additional visual cues, on the accuracy of such charts for proportion estimates. Since mobile devices are becoming the primary devices for casual reading, we performed all our experiments on such device. Moreover, the screen size of mobile devices is limited and it is therefore important to know how such size constraint affects the proportion accuracy. For this reason, in our first experiment we tested the chart size and we found that it has no significant effect on proportion accuracy. In our second experiment, we focused on the effect of the doughnut chart inner radius and we found that the proportion accuracy is insensitive to the inner radius, except the case of the thinnest doughnut chart. In the third experiment, we studied the effect of visual cues and found that marking the centre of the doughnut chart or adding tick marks at 25% intervals improves the proportion accuracy. Based on the results of the three experiments, we discuss the design of doughnut charts and offer suggestions for improving the accuracy of proportion estimates.

A genetically encoded, multifunctional photocrosslinker was developed for quantitative and comparative proteomics. By bearing a bioorthogonal handle and a releasable linker in addition to its photoaffinity warhead, this probe enables the enrichment of transient and low-abundance prey proteins after intracellular photocrosslinking and prey-bait separation, which can be subject to stable isotope dimethyl labeling and mass spectrometry analysis. This quantitative strategy (termed isoCAPP) allowed a comparative proteomic approach to be adopted to identify the proteolytic substrates of an E. coli protease-chaperone dual machinery DegP. Two newly identified substrates were subsequently confirmed by proteolysis experiments.

Kir2.1 (also known as IRK1) plays key roles in regulation of resting membrane potential and cell excitability. To achieve its physiological roles, Kir2.1 performs a series of conformational transition, named as gating. However, the structural basis of gating is still obscure. Here, we combined site-directed mutation, two-electrode voltage clamp with molecular dynamics simulations and determined that H221 regulates the gating process of Kir2.1 by involving a weak interaction network. Our data show that the H221R mutant accelerates the rundown kinetics and decelerates the reactivation kinetics of Kir2.1. Compared with the WT channel, the H221R mutation strengthens the interaction between the CD- and G-loops (E303-R221) which stabilizes the close state of the G-loop gate and weakens the interactions between C-linker and CD-loop (R221-R189) and the adjacent G-loops (E303-R312) which destabilizes the open state of G-loop gate. Our data indicate that the three pairs of interactions (E303-H221, H221-R189 and E303-R312) precisely regulate the G-loop gate by controlling the conformation of G-loop. Proteins 2016; 84:1929-1937. © 2016 Wiley Periodicals, Inc.

Activated cdc42-associated tyrosine kinase 1 (ACK1) has been reported to be implicated in non-small-cell lung cancer (NSCLC). However, the expression pattern and biological functions of ACK1 in the progression of NSCLC are not fully understood. In this study, it was found that the expression of ACK1 was significantly up-regulated in NSCLC samples compared to their adjacent normal tissues. Meanwhile, the expression of ACK1 was inversely correlated with the survival of NSCLC patients. Moreover, in the biological function studies, ACK1 was further validated to promote the growth, migration, and metastasis of NSCLC cells in vitro and in vivo. Mechanistically, ACK1 bind with FGFR1 and was essential for the phosphorylation of AKT induced by FGF. Our study demonstrated that ACK1 played an oncogenic role in the progression of NSCLC and ACK1 might be a promising target for the treatment of NSCLC. © 2015 Wiley Periodicals, Inc.

SummaryBackground The relationships between primary sclerosing cholangitis (PSC) and the environment are largely unknown. Aim To validate associations reported in previous studies and to identify novel environmental exposures among PSC patients. Methods We performed a multicenter, case–control analysis utilising self-administered questionnaires. Responses between cases (n = 1000) and controls (n = 663) were compared using multivariable logistic regression adjusted for age and gender. The model was further stratified based on inflammatory bowel disease (IBD) status (with IBD n = 741 without IBD n = 259). Results Smoking was associated with PSC only when IBD was present (OR, 0.5; 95% CI 0.4–0.7) but not among those PSC patients without IBD (OR, 0.9; 95% CI 0.7–1.2). Compared to controls, women with PSC (irrespective of the presence of IBD) were less likely to have received hormone replacement therapy (HRT; OR, 0.5; 95% CI 0.4–0.7) and were more likely to have recurrent urinary tract infections (OR, 1.6; 95% CI 1.2–2.3). PSC patients regardless of gender or IBD status were less likely to eat fish (OR, 0.4; 95% CI 0.3–0.6) and grilled/barbecued meat (OR, 0.8; 95% CI 0.7–0.9). In contrast, PSC patients with and without IBD were more likely to consume steak/burgers that were more well done (OR, 1.3; 95% CI 1.2–1.5). Conclusions IBD (rather than PSC) is associated with smoking. Women with PSC are more likely to have recurrent urinary tract infections and less likely to receive HRT. Dietary intake and methods of food preparation differ in PSC patients when compared to controls.

Cysteine proteinases (Cyps) play vital roles in many biological processes, including physiological and pathological reactions. In the present study, we cloned a full cDNA of SlCyp, encoding a 344-amino-acid protein from Spodoptera litura. The putative amino acid sequence shared >75% identity with Cyps from other insects. A phylogenetic analysis revealed that SlCyp is closely related to other known lepidopteran Cyps. Real-time PCR and Western blotting analyses showed that SlCyp is induced by Nomuraea rileyi infection in all the tissues tested. The strongest SlCyp mRNA and protein expression was found in haemocytes, followed by the fat bodies, of unchallenged and N. rileyi-challenged S. litura. A time-course analysis showed that SlCyp mRNA and protein expression levels were upregulated in the haemocytes and fat bodies by N. rileyi infection. Upon N. rileyi infection, the proteolytic activities of SlCyp were also significantly higher in the haemolymph than in normal or phosphate-buffered-saline-challenged controls. These results suggest that SlCyp plays an important role in the innate immunity of S. litura in response to N. rileyi. SlCyp mRNA and protein expression and activities were also elevated during sixth-instar moulting and metamorphosis. Knocking down SlCyp transcripts with double-stranded RNA interference caused prepupal, pupal, and adult phenotypic changes, and SlCyp-silenced mutant larvae displayed a significantly lower survival rate after N. rileyi infection. These facts suggest that SlCyp plays a significant role in resisting N. rileyi infection and an essential role in larval development. Our data should facilitate the development of techniques for S. litura control.

Twisted carbon fiber (TCF) aerogel with good selective sorption is produced in large scale by using raw cotton as the precursor. TCF aerogel shows highly efficient sorption of organic liquids (pump oil: up to 192 times its own weight; chloroform: up to 115 times its own weight). Moreover, it could be regenerated many times without decrease of sorption capacity by distillation, combustion or squeezing, which depends on the type of pollutants.

The interactions of three cationic distyryl dyes, namely 2,4-bis(4-dimethylaminostyryl)-1-methylpyridinium (1a), its derivative with a quaternary aminoalkyl chain (1b), and the symmetric 2,6-bis(4-dimethylaminostyryl)-1-methylpyridinium (2a), with several quadruplex and duplex nucleic acids were studied with the aim to establish the influence of the geometry of the dyes on their DNA-binding and DNA-probing properties. The results from spectrofluorimetric titrations and thermal denaturation experiments provide evidence that asymmetric (2,4-disubstituted) dyes 1a and 1b bind to quadruplex DNA structures with a near-micromolar affinity and a fair selectivity with respect to double-stranded (ds) DNA [K(a)(G4)/K(a)(ds)=2.5-8.4]. At the same time, the fluorescence of both dyes is selectively increased in the presence of quadruplex DNAs (more than 80-100-fold in the case of human telomeric quadruplex), even in the presence of an excess of competing double-stranded DNA. This optical selectivity allows these dyes to be used as quadruplex-DNA-selective probes in solution and stains in polyacrylamide gels. In contrast, the symmetric analogue 2a displays a strong binding preference for double-stranded DNA [K(a) (ds)/K(a) (G4)=40-100), presumably due to binding in the minor groove. In addition, 2a is not able to discriminate between quadruplex and duplex DNA, as its fluorescence is increased equally well (20-50-fold) in the presence of both structures. This study emphasizes and rationalizes the strong impact of subtle structural variations on both DNA-recognition properties and fluorimetric response of organic dyes.

Graphene nanopores expand when pore diameter is larger than membrane thickness after heat treatment; otherwise, nanopore size shrinks. Such size-dependent evolutionary mechanism of nanopores is considered as thermal-induced migration of uncombined carbon atoms. The amount of carbon adatoms determines the extent of diameter change. This could provide an applicable strategy for nanopore fabrication.

has attracted attention due to its fascinating properties such as high carrier mobility, [ 6–8 ] high thermal conductivity, [ 9 , 10 ] extraordinary elasticity and stiffness [ 11 ] and other properties. While mechanical exfoliation, [ 6 ] liquid exfoliation, [ 12 ] and epitaxial growth [ 13 ] can produce pristine graphene, graphene yields are currently too low for large-scale production of macrostructures. In contrast, chemical reduction of graphene oxide provides ‘graphene’ sheets in large scale for graphene macrostructures. [ 14–16 ] Graphene-based macrostructures prepared to date have been relatively weak mechanically, given their fl exible and often relatively porous or open structures, [ 17–26 ] particularly with respect to compressive strength when compared with commercial graphite products. [ 27–29 ] Achieving highly compacted and thus “fully dense” macrostructures based on graphene and measuring the physical properties of such material(s) is thus an important goal. Here, we report a pH-mediated hydrothermal reduction which is combined with moulding methods and allows controllable fabrication of compact high density graphene macrostructures with various shapes. The compact graphene (CG) product that is fabricated in this study shows great advantages over hitherto reported 3-D graphene products, [ 17–26 ] e.g. , a solid microstructure and a high density ( ∼ 1.6 g cm − 3 ) which is comparable to conventional graphite products [ 27–29 ] and an ultrahigh compressive strength ( ∼ 361 Mpa) which is 6 times higher than

In this work, spongy graphene (SG), a shape-mouldable and nanoporous material with a high specific surface area used as a versatile and recyclable sorbent material, is proposed and studied. SG shows highly efficient absorption of not only petroleum products and fats, but also toxic solvents such as toluene and chloroform (up to 86 times of its own weight), requiring no further pretreatment, which is tens of times higher than that of conventional absorbers. Moreover, SG can be regenerated (>10 times) by heat treatment, yielding the full release of adsorbates (>99%). The present work suggests SG a widespread potential for applications in industry as well as topics regarding environmental protection.

Ultrathin graphitic-phase C3N4 nanosheets are prepared by a liquid exfoliation route from bulk C3N4 in water.

In this study, effects of diluting either protein or crystallization agents in the droplets on the success rate of protein crystallization was investigated. Diluting the crystallization agent was found to increase the success rate of protein crystallization. Theoretical analysis showed that, concentration ranges of both protein and crystallization agent that can be scanned during the vapor diffusion process are wider with diluting the crystallization agent than that without dilution, resulting in more opportunities for the crystallization solution to be in the nucleation zone. On the other hand, diluting protein could lead to controversial results depending on the location of the initial concentration relative to that of the nucleation zone in the phase diagram. The method of diluting the crystallization agent is therefore proposed as an alternative modification to the conventional vapor diffusion method for obtaining more crystallization conditions in protein crystallization screening. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)