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Your search keyword '"Wolf, J. M."' showing total 23 results
Start Over Author "Wolf, J. M." Publisher wiley
23 results on '"Wolf, J. M."'

19. Microbiological, immunological and molecular methods suitable for commercial detection and quantification of the blackleg pathogen, Erwinia carotovora subsp. atroseptica, on seed potato tubers: a review

Author

PÉROMBELON, M. C. M., primary, BERTHEAU, Y., additional, CAMBRA, M., additional, FRECHON, D., additional, LOPEZ, M. M., additional, NIEPOLD, F., additional, PERSSON, P., additional, SLETTEN, A., additional, TOTH, I. K., additional, VAN VUURDE, J. W. L., additional, and VAN DER WOLF, J. M., additional

Published
1998
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23. Gadolinium inhibits thymidine incorporation and induces apoptosis in chondrocytes.

Author

Greisberg JK, Wolf JM, Wyman J, Zou L, and Terek RM

Subjects
Animals, Cartilage, Articular cytology, Cattle, Cell Division drug effects, Cells, Cultured, Chondrocytes drug effects, Chondrocytes metabolism, Dose-Response Relationship, Drug, Proteoglycans biosynthesis, Tritium, Apoptosis drug effects, Chondrocytes cytology, Gadolinium toxicity, Thymidine pharmacokinetics
Abstract

Magnetic resonance arthrography. a procedure where contrast agents containing gadolinium are administered intra-articularly, has become a useful tool in musculoskeletal diagnosis. Although considered safe for systemic use, toxicities in some tissues have been identified for both free gadolinium ion and the gadolinium chelates used as contrast. In this study, the effects of short-term exposure of articular chondrocytes to gadolinium contrast were examined by assaying for proteoglycan synthesis, cell proliferation, and apoptosis. Bovine chondrocytes were grown in monolayer culture and exposed to gadodiamide for 16 h. Proteoglycan synthesis was measured through incorporation of radiolabeled sulfate. Uptake of radiolabeled thymidine assessed cell proliferation. Apoptosis was detected using the TUNEL assay, where DNA strand breaks characteristic of apoptosis are labeled with fluorescent nucleotide. Proteoglycan synthesis was stimulated by lower dose exposure to gadodiamide. At higher doses, proteoglycan synthesis returned to baseline. Cell proliferation decreased following exposure to gadodiamide in a dose-dependent manner. Chondrocyte apoptosis was induced in a dose-dependent manner. Further work is needed to determine if these in vitro effects are present in the intact joint.

Published
2001
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