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Your search keyword '"Woelders H"' showing total 10 results
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10 results on '"Woelders H"'

1. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal

Author

Ortiz-Escribano, N., primary, Bogado Pascottini, O., additional, Woelders, H., additional, Vandenberghe, L., additional, De Schauwer, C., additional, Govaere, J., additional, Van den Abbeel, E., additional, Vullers, T., additional, Ververs, C., additional, Roels, K., additional, Van De Velde, M., additional, Van Soom, A., additional, and Smits, K., additional

Published
2017
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2. Systems Biology and Livestock Science

Author

te Pas, M.F.W., Woelders, H., and Bannink, A.

Subjects
animals, livestock, computers, dieren, molecular biology, vee, systems biology, systeembiologie, moleculaire biologie
Abstract

Systems Biology is an interdisciplinary approach to the study of life made possible through the explosion of molecular data made available through the genome revolution and the simultaneous development of computational technologies that allow us to interpret these large data sets. Systems Biology has changed the way biological science views and studies life and has been implemented in research efforts across the biological sciences. Systems Biology and Livestock Science will be the first book to review the latest advances using this research methodology in efforts to improve the efficiency, health, and quality of livestock production. Systems Biology and Livestock Science opens with useful introductory chapters explaining key systems biology principles. The chapters then progress to look at specific advances in fields across livestock science. Coverage includes, but is not limited to, chapters on systems biology approaches to animal nutrition, reproduction, health and disease, and animal physiology. Written by leading researchers in the field, Systems Biology and Livestock Science, will be an invaluable resource to researchers, professionals, and advance students working in this rapidly developing discipline.

Published
2011

10. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal.

Author

Ortiz-Escribano N, Bogado Pascottini O, Woelders H, Vandenberghe L, De Schauwer C, Govaere J, Van den Abbeel E, Vullers T, Ververs C, Roels K, Van De Velde M, Van Soom A, and Smits K

Subjects
Animals, Dimethyl Sulfoxide administration & dosage, Dose-Response Relationship, Drug, Embryo Culture Techniques, Embryo Transfer, Female, Glycerol administration & dosage, Pregnancy, Tissue Preservation methods, Cryopreservation veterinary, Horses embryology, In Vitro Oocyte Maturation Techniques veterinary, Oocytes physiology, Tissue Preservation veterinary, Vitrification
Abstract

Background: The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised., Objectives: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes., Study Design: Experimental in vitro and in vivo trials., Methods: Immature equine oocytes were vitrified after a short exposure to high concentrations of cryoprotective agents (CPAs), or a long exposure to lower concentrations of CPAs. In Experiment 1, the maturation of oocytes surrounded by multiple layers of cumulus cells (CC oocytes) and oocytes surrounded by only corona radiata (CR oocytes) was investigated. In Experiment 2, spindle configuration was determined for CR oocytes vitrified using the two vitrification protocols. In Experiment 3, further embryonic development was studied after fertilisation and culture. Embryo transfer was performed in a standard manner., Results: Similar nuclear maturation rates were observed for CR oocytes vitrified using the long exposure and nonvitrified controls. Furthermore, a lower maturation rate was obtained for CC oocytes vitrified with the short exposure compared to control CR oocytes (P = 0.001). Both vitrification protocols resulted in significantly higher rates of aberrant spindle configuration than the control groups (P<0.05). Blastocyst development only occurred in CR oocytes vitrified using the short vitrification protocol, and even though blastocyst rates were significantly lower than in the control group (P<0.001), transfer of five embryos resulted in one healthy foal., Main Limitations: The relatively low number of equine oocytes and embryo transfer procedures performed., Conclusions: For vitrification of immature equine oocytes, the use of 1) CR oocytes, 2) a high concentration of CPAs, and 3) a short exposure time may be key factors for maintaining developmental competence., (© 2017 EVJ Ltd.)

Published
2018
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