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31 results on '"Wilson, Kelly"'

10. Microbe‐Host Crosstalk between Short‐Chain Fatty Acids and Intestinal Epithelial HIF Provides a New Mechanism to Augment Tissue Barrier Function

Author

Zheng, Leon, primary, Kelly, Caleb, additional, Campbell, Eric, additional, Saeedi, Bejan, additional, Scholz, Carsten, additional, Bayless, Amanda, additional, Wilson, Kelly, additional, Glover, Louise, additional, Kominsky, Douglas, additional, Magnuson, Aaron, additional, Weir, Tiffany, additional, Ehrentraut, Stefan, additional, Nguyen, Vu, additional, Taylor, Cormac, additional, and Colgan, Sean, additional

Published
2015
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30. Development of Youth-Centered Health Messaging Posters for High School-Based Health Clinics in Hawaii.

Author

McKay EA, Mattheus D, Garney WR, Wilson KL, and Fontenot HB

Subjects
Adult, Humans, Adolescent, Hawaii, Mental Health, Social Behavior, School Nursing, Sexual Health
Abstract

Background: Youth may be reluctant to seek health care from school health providers due to feeling embarrassed or stigmatized in the health office environment or worried about their confidentiality. The purpose of this project was to create a set of youth-centered health posters that promote youth engagement with nursing staff and to standardize health messaging across high schools in Hawaii school-based clinics., Methods: Two community advisory boards, 1 composed of 10 youth stakeholders (mean age 17 years) and the other of 7 adult stakeholders, informed poster development utilizing web-based discussion groups. The discussions were transcribed, and additional data was collected using field notes and anonymous digital messages. Adult advisory board members also provided feedback on suggested poster text through an online survey., Results: Youth and adult advisory board participants identified 4 key health concerns facing youth: confidentiality, sexual health, relationships, and mental health. Based on input from the 2 advisory boards, 4 posters were developed, each centered on 1 key health issue., Implications for School Health Policy, Practice, and Equity: School-based posters can convey important messages to help youth understand their health care rights and responsibilities, as well as identify important issues open for discussion with nurses., Conclusion: Posters are an underutilized tool for school health providers to create welcoming, inclusive health care environments and facilitate health-related conversations with youth. This paper describes participant feedback about the characteristics of a memorable poster and briefly outlines current knowledge and recommendations for school health providers regarding each of the 4 health issues., (© 2023, American School Health Association.)

Published
2024
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31. Cis-suppression to arrest protein aggregation in mammalian cells.

Author

Gregoire S, Zhang S, Costanzo J, Wilson K, Fernandez EJ, and Kwon I

Subjects
Amino Acid Substitution, Cell Line, Humans, Mutant Proteins chemistry, Mutant Proteins genetics, Mutant Proteins metabolism, Mutation, Missense, Superoxide Dismutase genetics, Superoxide Dismutase-1, Protein Denaturation, Protein Folding, Protein Multimerization, Superoxide Dismutase chemistry, Superoxide Dismutase metabolism
Abstract

Protein misfolding and aggregation are implicated in numerous human diseases and significantly lower production yield of proteins expressed in mammalian cells. Despite the importance of understanding and suppressing protein aggregation in mammalian cells, a protein design and selection strategy to modulate protein misfolding/aggregation in mammalian cells has not yet been reported. In this work, we address the particular challenge presented by mutation-induced protein aggregation in mammalian cells. We hypothesize that an additional mutation(s) can be introduced in an aggregation-prone protein variant, spatially near the original mutation, to suppress misfolding and aggregation (cis-suppression). As a model protein, we chose human copper, zinc superoxide dismutase mutant (SOD1(A4V) ) containing an alanine to valine mutation at residue 4, associated with the familial form of amyotrophic lateral sclerosis. We used the program RosettaDesign to identify Phe20 in SOD1(A4V) as a key residue responsible for SOD1(A4V) conformational destabilization. This information was used to rationally develop a pool of candidate mutations at the Phe20 site. After two rounds of mammalian-cell based screening of the variants, three novel SOD1(A4V) variants with a significantly reduced aggregation propensity inside cells were selected. The enhanced stability and reduced aggregation propensity of the three novel SOD1(A4V) variants were verified using cell fractionation and in vitro stability assays., (© 2013 Wiley Periodicals, Inc.)

Published
2014
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