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Your search keyword '"Wade, K. A."' showing total 77 results
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77 results on '"Wade, K. A."'

1. Protein kinetics of superoxide dismutase‐1 in familial and sporadic amyotrophic lateral sclerosis

Author

Cindy V. Ly, Margaret D. Ireland, Wade K. Self, James Bollinger, Jennifer Jockel‐Balsarotti, Hillary Herzog, Peggy Allred, Leah Miller, Michael Doyle, Isabel Anez‐Bruzual, Bhavesh Trikamji, Ted Hyman, Tyler Kung, Katherine Nicholson, Robert C. Bucelli, Bruce W. Patterson, Randall J. Bateman, and Timothy M. Miller

Subjects
Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Objective Accumulation of misfolded superoxide dismutase‐1 (SOD1) is a pathological hallmark of SOD1‐related amyotrophic lateral sclerosis (ALS) and is observed in sporadic ALS where its role in pathogenesis is controversial. Understanding in vivo protein kinetics may clarify how SOD1 influences neurodegeneration and inform optimal dosing for therapies that lower SOD1 transcripts. Methods We employed stable isotope labeling paired with mass spectrometry to evaluate in vivo protein kinetics and concentration of soluble SOD1 in cerebrospinal fluid (CSF) of SOD1 mutation carriers, sporadic ALS participants and controls. A deaminated SOD1 peptide, SDGPVKV, that correlates with protein stability was also measured. Results In participants with heterozygous SOD1A5V mutations, known to cause rapidly progressive ALS, mutant SOD1 protein exhibited ~twofold faster turnover and ~ 16‐fold lower concentration compared to wild‐type SOD1 protein. SDGPVKV levels were increased in SOD1A5V carriers relative to controls. Thus, SOD1 mutations impact protein kinetics and stability. We applied this approach to sporadic ALS participants and found that SOD1 turnover, concentration, and SDGPVKV levels are not significantly different compared to controls. Interpretation These results highlight the ability of stable isotope labeling approaches and peptide deamidation to discern the influence of disease mutations on protein kinetics and stability and support implementation of this method to optimize clinical trial design of gene and molecular therapies for neurological disorders. Trial Registration Clinicaltrials.gov: NCT03449212.

Published
2023
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2. Protein kinetics of superoxide dismutase‐1 in familial and sporadic amyotrophic lateral sclerosis

Author

Ly, Cindy V., primary, Ireland, Margaret D., additional, Self, Wade K., additional, Bollinger, James, additional, Jockel‐Balsarotti, Jennifer, additional, Herzog, Hillary, additional, Allred, Peggy, additional, Miller, Leah, additional, Doyle, Michael, additional, Anez‐Bruzual, Isabel, additional, Trikamji, Bhavesh, additional, Hyman, Ted, additional, Kung, Tyler, additional, Nicholson, Katherine, additional, Bucelli, Robert C., additional, Patterson, Bruce W., additional, Bateman, Randall J., additional, and Miller, Timothy M., additional

Published
2023
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3. Protein production is an early biomarker for RNA ‐targeted therapies

Author

Self, Wade K., primary, Schoch, Kathleen M., additional, Alex, Jacob, additional, Barthélemy, Nicolas, additional, Bollinger, James G., additional, Sato, Chihiro, additional, Cole, Tracy, additional, Kordasiewicz, Holly B., additional, Swayze, Eric, additional, Bateman, Randall J., additional, and Miller, Timothy M., additional

Published
2018
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5. Video compression for multicast environments using spatial scalability and simulcast coding

Author

Xuemin Chen, Ajay Luthra, and Wade K Wan

Subjects
Multicast, business.industry, Computer science, computer.file_format, ENCODE, Scalable Video Coding, Electronic, Optical and Magnetic Materials, MPEG-4, Bit allocation, Computer Vision and Pattern Recognition, Electrical and Electronic Engineering, business, Spatial scalability, computer, Software, Computer network, Data compression, Coding (social sciences)
Abstract

An optimal system for determining whether simulcast coding (400) or spatial scalability coding (100) should be used to encode video for clients with a specific communication link. Operating points (A', B', C') for both simulcast coding and spatial scalability are also determined. Adaptive switching (3130, 3140) is provided, with the operating points and decision boundaries being used to guide the switching to optimize the quality of the higher-resolution data based on a bit allocation to the associated lower-resolution data. A system for determining the point (A', C') of equal quality in both layers of simulcast and spatial scalability coding is also disclosed. The proportion of bits allocated to the base layer to achieve equal quality is essentially independent of the total bit rate for both simulcast and spatial scalability.

Published
2003

6. POPULATION REGULATION IN AN AQUATIC INSECT: THE ROLE OF DISEASE

Author

Wade K. Hoiland and Steven L. Kohler

Subjects
Larva, education.field_of_study, Density dependence, Ecology, Delayed density dependence, Voltinism, Population, Prevalence, Parasitism, Biology, education, Population density, Ecology, Evolution, Behavior and Systematics
Abstract

We studied the dynamics of a univoltine stream-dwelling caddisfly, Brachy- centrus americanus, and its microsporidian disease for 15 yr. The pathogen is highly vir- ulent, with infections generally resulting in death during the larval stage. In most years, we sampled Brachycentrus immatures every 1-2 mo to estimate population density and disease prevalence. Brachycentrus population dynamics exhibited strong evidence for direct and delayed (1 generation lag) density dependence and appeared to cycle with a period of -4 yr. Pathogen prevalence in Brachycentrus was >10% in all generations and frequently exceeded 50%. Disease prevalence also exhibited cyclic behavior, with peaks in prevalence following Brachycentrus generations with relatively high population density. There was no evidence for direct density-dependent parasitism, but there was strong evidence for density- dependent infection delayed by one generation. A path analysis model suggested that the disease mediated much of the strong density dependence observed in.Brachycentrus. Col- lectively, these observations suggest that the pathogen is largely responsible for driving observed Brachycentrus dynamics.

Published
2001

7. Effects of tamoxifen on telomerase activity in breast carcinoma cell lines

Author

A B S Louis Matej, Wade K. Aldous, J B A Amber Marean, Katherine H. Moore, and Mary J. Dehart

Subjects
Cancer Research, Telomerase, medicine.medical_specialty, Cell growth, Cell, Estrogen receptor, Biology, Antiestrogen, medicine.anatomical_structure, Endocrinology, Oncology, Cell culture, Internal medicine, Cancer research, medicine, Telomerase reverse transcriptase, skin and connective tissue diseases, Tamoxifen, medicine.drug
Abstract

BACKGROUND The authors tested the effects of the antiestrogenic agent tamoxifen on telomerase activity and cell proliferation in MCF-7 and MDA-MB-231 breast carcinoma cell lines. MCF-7 cells belong to a known estrogen receptor positive cell line, whereas MDA-MB-231 cells, previously thought to be estrogen receptor negative, are now shown to contain estrogen receptor-β. METHODS Both cell lines were grown in the presence of tamoxifen 10−6, 10−7, 10−8, and 10−9 M for 10 days. Cells in separate flasks were harvested daily for determination of total cell number, protein was extracted for determination of telomerase activity, and RNA was extracted for reverse transcriptase–polymerase chain reaction analysis to measure expression levels of the telomerase components (the RNA component and the catalytic subunit) and estrogen receptors. RESULTS Total cell counts and telomerase activity levels of both cell lines with 10−8 M tamoxifen treatment were lower than control cells and other tamoxifen treatments. Changes in the expression of individual telomerase components correlated with telomerase activity. Estrogen receptor status did not correlate with telomerase activity. CONCLUSIONS Tamoxifen strongly affected both cell count and telomerase activity within the 10−8 M concentration of both cell lines. Cells were able to overcome drug inhibition at all other doses after 4 days. Telomerase activity and cell proliferation were correlated in both cell lines and depended on drug concentration. Tamoxifen showed long term effects on cell proliferation of the MCF-7 cells. Cancer 1999;85:1523–9. © 1999 American Cancer Society.

Published
1999

9. Recovery of macroinvertebrate communities from metal pollution in the South Fork and mainstem of the Coeur d'Alene River, Idaho

Author

Fred W. Rabe, Wade K. Hoiland, and Russell C. Biggam

Subjects
Hydrology, Ecological Modeling, Species diversity, Biota, Pollution, Tailings, Habitat, Benthic zone, Environmental Chemistry, Environmental science, Species richness, Water pollution, Waste Management and Disposal, Biotic potential, Water Science and Technology
Abstract

Mining has severely impacted the biota in the Coeur d'Alene River drainage of northern Idaho. The benthic community of the South Fork and mainstem was monitored from 1968 to 1971 and from 1987 to 1991 to determine the effects of improved mine wastewater treatment and mine closures. With the decrease of heavy metal levels, the benthic community showed a gradual recovery as evidenced by large increases in taxonomic richness (0 to 18), Ephemeroptera-Plecoptera-Trichoptera index (0 to 8), and species diversity (0 to 1.8). Although considerable recovery has occurred, the biotic integrity of impacted sites continues to lag behind reference sites, probably due to poor habitat structure and metals leaching from mine tailings

Published
1994

30. Microcapillary agglutination assay for detection of specific antileukocyte reactivity in neutropenic patients

Author

Nadine N. Burton, Susan L. Tseng, James W. Mold, and Wade K. Smith

Subjects
Adult, Male, Neutropenia, Neutrophils, Human leukocyte antigen, medicine.disease_cause, Autoimmunity, Antigen, HLA Antigens, Agglutination Tests, Direct agglutination test, Leukocytes, medicine, Humans, Blood Transfusion, Antigens, Aged, Autoantibodies, business.industry, Hematology, Middle Aged, Cytotoxicity Tests, Immunologic, medicine.disease, Autoimmune neutropenia, Immunology, Agglutination assay, Female, business, Agranulocytosis
Abstract

Serum leukoagglutinating activity against the leukocytes of four patients with neutropenia was demonstrated using a modified microcapillary agglutination test. Cells from a panel of donors proved useful in attempting to define the identity of the antigens involved. In one instance anti-HLA-A9 activity could be demonstrated in a patient possessing HLA-A9. In the other three individuals no definite antigen assessment to HLA Series A and B antigens or the Lalezari series of neutrophil antigens could be made. Two of the patients' sera showed cross-reactivity and may be reactive with the same antigen or antigenic group. The microcapillary agglutination test appears to be useful in the evaluation of possible cases of autoimmune neutropenia.

Published
1979

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