1. Generation and Characterization of Patient‐Derived Head and Neck, Oral, and Esophageal Cancer Organoids
- Author
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Joel Gabre, Jianwen Que, Angela J. Yoon, Ricardo Cruz-Acuna, Gary W. Falk, Shoji Natsugoe, Adam J. Bass, Julian A. Abrams, Tatiana A. Karakasheva, Lorenzo Ferri, Veena Sangwan, Anil K. Rustgi, Timothy C. Wang, Hiroshi Nakagawa, Varun Sahu, Kelly A. Whelan, Gregory G. Ginsberg, Elizabeth Philipone, J. Alan Diehl, Veronique Giroux, Hisatsugu Maekawa, Andres J. Klein-Szanto, Devraj Basu, Masataka Shimonosono, and Takashi Kijima
- Subjects
0301 basic medicine ,Drug ,Esophageal Neoplasms ,media_common.quotation_subject ,Primary Cell Culture ,Cell ,Biology ,Article ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Organoid ,medicine ,Humans ,Precision Medicine ,Cells, Cultured ,media_common ,medicine.diagnostic_test ,business.industry ,Histology ,Cell Biology ,General Medicine ,Esophageal cancer ,medicine.disease ,Organoids ,030104 developmental biology ,medicine.anatomical_structure ,Cancer research ,Adenocarcinoma ,Esophageal Squamous Cell Carcinoma ,Personalized medicine ,business ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Esophageal cancers comprise adenocarcinoma and squamous cell carcinoma, two distinct histologic subtypes. Both are difficult to treat and among the deadliest human malignancies. We describe protocols to initiate, grow, passage, and characterize patient-derived organoids (PDO) of esophageal cancers, as well as squamous cell carcinomas of oral/head-and-neck and anal origin. Formed rapidly (
- Published
- 2020