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Polymerization of 3',5' cyclic guanosine monophosphate (3',5' cGMP) was previously reported to afford short RNA sequences from a plausible mildly activated prebiotic precursor in a non-enzymatic and template-free manner. In the current paper we analyze the reaction with PAGE as well as by infrared micro-and nanospectroscopy on time scales not considered before. We provide evidence that a preliminary oligomerization step in drystate allows for a continued synthesis of RNA oligomers even in the presence of subsequently added water. On a longer time scale, the oligomerization yield oscillates in an apparently chaotic fashion hinting at the importance of some non-reversible phase-separation processes on the experimentally observed outcome of the polymerization/degradation chemistries. The long-lasting (hundreds of hours) intrinsic sustainability and resilience of the process gives a robust prebiotic potential to this unique oligomerization reaction, which could lead to the very first oligonucleotide sequences on the primordial Earth.

International audience; Functionalized scanning probe tips hold great promise for the controlled delivery of signals from and to selected nanoscale volumes. Here, a new nanotechnological approach for the functionalization of scanning probe tips is presented, targeting the transfer of the optical, electrical, thermal, or chemical properties of precisely characterized epitaxial semiconductor layers to the nanoscale probe tip. Homogeneously doped, strain-relaxed heteroepitaxial germanium layers, several micrometers thick, are first grown on silicon wafers by low-energy plasma-enhanced chemical vapor deposition and then employed to functionalize the probe apex. This choice of materials and growth technique enables the future scalability of the tip functionalization process toward high production volumes. The fabricated probe tips are investigated in a transmission electron microscope, revealing that the crystal structure and the homogeneous doping level of the epitaxial layers are unchanged after the probe-tip functionalization process. These doped-germanium tips are also tested as nanoemitters of light at telecom wavelengths (1.55 µm) and applied as scattering probes for near-field mid-infrared microscopy. The reported combination of heteroepitaxial growth and a nanofabrication approach can be extended to a variety of epitaxial materials, thus enabling a new generation of scanning probes for the investigation of nanostructured materials and devices.