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A saturated riparian buffer (SRB) is an edge-of-field conservation practice that intercepts tile drainage and reduces nitrate flux to nearby streams by redistributing the flow as shallow groundwater. In this study, a three-dimensional, finite-difference groundwater model representative of SRBs in central Iowa was developed to assess the flow of groundwater and implications for nitrate removal during spring conditions, when flow to the SRB is highest. The model reproduces field observations of water level with Nash-Sutcliffe efficiency of 0.68, which is deemed acceptable for hydrologic models. The modeling shows that groundwater flow is three-dimensional near the distribution pipe and the stream and primarily one-dimensional in the rest of the buffer. The path the water takes in flowing toward the stream depends on where it exits the distribution pipe. When nitrate is not limiting, the potential for nitrate removal depends on the length of the path-and thus travel time-and depth because denitrification potential varies with depth. Travel time T

A saturated riparian buffer (SRB) is an edge-of-field conservation practice that reduces nitrate export from agricultural lands by redistributing tile drainage as shallow groundwater and allowing for denitrification and plant uptake. We propose an approach to improve the design of SRBs by analyzing a tradeoff in choosing the SRB width, and we apply the approach to six sites with SRBs in central Iowa. A larger width allows for more residence time, which increases the opportunity for removing nitrate that enters the buffer. However, because the SRBs considered here treat only a portion of the tile flow when it is large, for the same difference in hydraulic head, a smaller width allows more of the total tile flow to enter the buffer and therefore treats more of the drainage. By maximizing the effectiveness of nitrate removal, defined as the ratio of total nitrate removed by the SRB to total nitrate leaving the field in tile drainage, an equation for the optimal width was derived in terms of soil properties, denitrification rates, and head difference. All six sites with existing SRBs considered here have optimal widths smaller than the current width, and two are below the minimum width listed in current design standards. In terms of uncertainty, the main challenges in computing the optimal width for a site are estimating the removal coefficient for nitrate and determining the saturated hydraulic conductivity. Nevertheless, including a width that accounts for site conditions in the design standards would improve water quality locally and regionally.

The objective of this study was to develop a conceptual framework to study soil aggregate dynamics through the integration of aggregation, disruption, stabilization and destabilization processes of soil constituent units. We used aggregate‐size distribution and water stability of macroaggregates to evaluate changes in soil aggregates in two ecosystems where one is the ‘reference’ state and the other is the ‘new’ state. The framework was validated with data from (a) a non‐buffered annual row crop (ARC) system, (b) a no‐till (NT) system and (c) a bare fallow (BF) system. The dynamics of soil aggregates in the ARC were dominated by the disruption of aggregates (the aggregation–disruption index (ADI) was −0.08), in NT they were dominated by the aggregation process (ADI = 0.03) and in BF dominated by the disruption of aggregates (ADI = −0.20). Three pathways were observed: (a) expression of the aggregation process, which is essential in reestablishing soil structure and functionality, (b) expression of the disruption process, which is critical in the destruction of soil structure and (c) prevalence of mixed behaviour, where the dominant process depended on the relative rate of aggregation and disruption. The proposed framework and study of the carbon associated with aggregates could pave the way for the development of a methodology for the quantitative study of soil organic carbon (SOC) dynamics, carbon inputs (outputs), soil carbon fractions, and edaphic and biotic conditions that regulate SOC dynamics. HIGHLIGHTS: Soil aggregate dynamics were assessed within multiple management systems. A new methodology is proposed for the quantitative study of soil aggregate dynamics. Provided an understanding of aggregation, disruption, stabilization and destabilization in aggregate dynamics. Aggregate dynamic pathways of value in land management can minimize deterioration of the environment.

Nitrate from artificial drainage pipes (tiles) underlying agricultural fields is a major source of reactive N, especially NO, in surface waters. A novel approach for reducing NO loss is to intercept a field tile where it crosses a riparian buffer and divert a fraction of the flow as shallow groundwater within the buffer. This practice is called a saturated riparian buffer (SRB), and although it is promising, little data on the performance of the practice is available. This research investigated the effectiveness of SRBs in removing NO at six sites installed across Iowa, resulting in a total of 17 site-years. Water flow and NO in the tile outlets, diverted into the buffers, and NO concentration changes within the buffers were monitored throughout the year at each site. Results showed that all the SRBs were effective in removing NO from the tile outlet, with the average annual NO load removal ranging from 13 to 179 kg N for drainage areas ranging from 3.4 to 40.5 ha. This is NO that would have otherwise discharged directly into the adjoining streams. The annual removal effectiveness, which is the total NO removed in the SRB divided by the total NO draining from the field, ranged from 8 to 84%. This corresponds to an average removal rate of 0.040 g N m d with a range of 0.004 to 0.164 g N m d. Assuming a 40-yr life expectancy for the structure and a 4% discount rate, we computed a mean equal annual cost for SRBs of US$213.83. Given the average annual removal of 73 kg for all site-years, this cost equates to $2.94 kg N removed, which is very competitive with other field-edge practices such as denitrification bioreactors and constructed wetlands. Thus, SRBs continue to be a promising practice for NO removal in tile-drained landscapes.

Excess NO leaching from the agricultural Midwest via tile drainage water has contributed to both local drinking water and national Gulf of Mexico benthic hypoxia concerns. Both in-field and edge-of-field practices have been designed to help mitigate NO flux to surface waters. Edge-of-field practices focus on maximizing microbial denitrification, the conversion of NO to N gas. This study assessed denitrification rates from two saturated riparian buffers (SRBs) for 2 yr and a third SRB for 1 yr, for a total of five sample years. These SRBs were created by diverting NO-rich tile drainage water into riparian buffers soils. The SRBs in this study removed between 27 and 96% of the total diverted NO load. Measured cumulative average denitrification rate for each SRB sample year accounted for between 3.7 and 77.3% of the total NO removed. Both the cumulative maximum and 90% confidence interval denitrification rates accounted for all of the NO removed by the SRBs in three of the five sample years, indicating that denitrification can be a dominant NO removal mechanism in this edge-of-field practice. When adding the top 20 cm of each core to the cumulative denitrification rates for each SRB, denitrification accounted for between 33 and over 100% of the total NO removed. Buffer age (time since establishment) was speculated to enhance denitrification rates, and there was a trend of the soil closer to the surface making up the majority of the total denitrification rate. Finally, both NO and C could limit denitrification in these SRBs.

Quantification of soil gas flux using the static chamber method is labor intensive. The number of chambers that can be sampled is limited by the spacing between chambers and the availability of trained research technicians. An automated system for collecting gas samples from chambers in the field would eliminate the need for personnel to return to the chamber during a flux measurement period and would allow a single technician to sample multiple chambers simultaneously. This study describes hamber utomated ampling quipment (Flux) to collect and store chamber headspace gas samples at assigned time points for the measurement of soil gas flux. The FluxCASE design and operation is described, and the accuracy and precision of the FluxCASE system is evaluated. In laboratory measurements of nitrous oxide (NO), carbon dioxide (CO), and methane (CH) concentrations of a standardized gas mixture, coefficients of variation associated with automated and manual sample collection were comparable, indicating no loss of precision. In the field, soil gas fluxes measured from FluxCASEs were in agreement with manual sampling for both NO and CO. Slopes of regression equations were 1.01 for CO and 0.97 for NO. The 95% confidence limits of the slopes of the regression lines included the value of one, indicating no bias. Additionally, an expense analysis found a cost recovery ranging from 0.6 to 2.2 yr. Implementing the FluxCASE system is an alternative to improve the efficiency of the static chamber method for measuring soil gas flux while maintaining the accuracy and precision of manual sampling.

Summary Agriculture in the temperate zone of the Midwestern USA has removed most riparian buffer zones from the landscape, which impairs the soil functioning of the soil in these ecosystems. The objective of this study was to assess soil degradation in a non-buffered riparian zone with an annual row crop (ARC) and in a longer established (more than 85 years) riparian cool season grass (CSG) buffer in central Iowa, USA. To achieve our objective, we compared the functioning of the target system, ARC, with a system that has been undisturbed since 1990, CSG. We measured the aggregate size distribution and stability of macroaggregrates, aggregate-associated soil organic carbon and particulate organic matter (POM). The categorization of aggregates by size and water stability (slaking resistance) showed a significantly larger (P 250 µm) under CSG (53%) than under ARC (26.3%). On the other hand, soil under ARC had the larger proportion of large and small water-unstable macroaggregates. The results indicate that the concentration of organic C associated with the total water-stable aggregates is 1.9 times greater under CSG (23.9 mg C g−1) than for the ARC (12.4 mg C g−1). The concentration of carbon in light particulate organic matter (LPOM-C) under CSG accounted for 12% of total soil organic carbon (TSOC), but only 6% under ARC management. The chemical composition of LPOM in the ARC soil had a large alkyl-C/O-alkyl C ratio, which suggests more organic matter decomposition than with the CSG. Electronic micrographs showed evidence that under CSG roots and fungal hyphae form an extensive network around the large macroaggregates (> 2000 µm), and thus maintain the stability of the macroaggregates. Preserving the CSG buffer reduces soil erosion and the processes associated with nonpoint source pollutants, which improves water quality in an intensively managed agricultural system. Highlights How do soil aggregation and POM change after conversion of CSG buffer to continuous cultivation? Improved understanding of soil degradation through water-stable and -unstable aggregates and SOC. Lack of disturbance of CSG buffer favours formation of macroaggregates and SOC stabilization. Preserving CSG buffer improves water quality in an intensively managed agricultural system.

Strategies to reduce nitrate-nitrogen (nitrate) pollution delivered to streams often seek to increase groundwater residence time to achieve measureable results, yet the effects of tile drainage on residence time have not been well documented. In this study, we used a geographic information system groundwater travel time model to quantify the effects of artificial subsurface drainage on groundwater travel times in the 7443-ha Bear Creek watershed in north-central Iowa. Our objectives were to evaluate how mean groundwater travel times changed with increasing drainage intensity and to assess how tile drainage density reduces groundwater contributions to riparian buffers. Results indicate that mean groundwater travel times are reduced with increasing degrees of tile drainage. Mean groundwater travel times decreased from 5.6 to 1.1 yr, with drainage densities ranging from 0.005 m (7.6 mi) to 0.04 m (62 mi), respectively. Model simulations indicate that mean travel times with tile drainage are more than 150 times faster than those that existed before settlement. With intensive drainage, less than 2% of the groundwater in the basin appears to flow through a perennial stream buffer, thereby reducing the effectiveness of this practice to reduce stream nitrate loads. Hence, strategies, such as reconnecting tile drainage to buffers, are promising because they increase groundwater residence times in tile-drained watersheds.

Tufekcioglu, Mustafa, Richard C. Schultz, George N. Zaimes, Thomas M. Isenhart, and Aydin Tufekcioglu, 2012. Riparian Grazing Impacts on Streambank Erosion and Phosphorus Loss via Surface Runoff. Journal of the American Water Resources Association (JAWRA) 1-11. DOI: 10.1111/j.1752-1688.2012.12004.x Abstract: Surface runoff is one of the major pathways of sediment and phosphorus (P) transport to surface waters. Rainfall simulations were conducted on nine grazed pasture sites with different stocking rates in three different Iowa (United States) regions. The purpose of the simulations was to determine the impacts of cattle grazing on the amounts of sediment and P in surface runoff within a 15-m wide strip on both sides of the stream from different source areas (SAs). These riparian SAs included stream-side loafing areas, cattle streambank access paths to the stream, and the other vegetated areas adjacent to the streambanks. The runoff samples collected during the simulations were analyzed for suspended sediment (SS) and total phosphorus (TP). Soil bulk density and antecedent soil moisture samples were collected around the rainfall simulation plots to identify differences in compaction, infiltration, and surface runoff among the SAs. SS and TP losses from access paths and loafing areas within the 15-m wide strips accounted for up to 72 and 55% of the total losses, respectively, even though they accounted for only 2.7% of the total area within the 15-m wide strips. This suggests that access paths and loafing areas require special attention to mitigate the impacts of cattle on stream water pollution. Significant correlations were found between stocking rates and both SS and TP losses suggesting that low stocking rates can reduce sediment and P export to streams from the SAs.

Schilling, Keith E., Thomas M. Isenhart, Jason A. Palmer, Calvin F. Wolter, and Jean Spooner, 2011. Impacts of Land-Cover Change on Suspended Sediment Transport in Two Agricultural Watersheds. Journal of the American Water Resources Association (JAWRA) 47(4):672-686. DOI: 10.1111/j.1752-1688.2011.00533.x Abstract: Suspended sediment is a major water quality problem, yet few monitoring studies have been of sufficient scale and duration to assess the effectiveness of land-use change or conservation practice implementation at a watershed scale. Daily discharge and suspended sediment export from two 5,000-ha watersheds in central Iowa were monitored over a 10-year period (water years 1996-2005). In Walnut Creek watershed, a large portion of land was converted from row crop to native prairie, whereas in Squaw Creek land use remained predominantly row crop agriculture. Suspended sediment loads were similar in both watersheds, exhibiting flashy behavior typical of incised channels. Modeling suggested that expected total soil erosion in Walnut Creek should have been reduced 46% relative to Squaw Creek due to changes in land use, yet measured suspended sediment loads showed no significant differences. Stream mapping indicated that Walnut Creek had three times more eroding streambank lengths than did Squaw Creek suggesting that streambank erosion dominated sediment sources in Walnut Creek and sheet and rill sources dominated sediment sources in Squaw Creek. Our results demonstrate that an accounting of all sources of sediment erosion and delivery is needed to characterize sediment reductions in watershed projects combined with long-term, intensive monitoring and modeling to account for possible lag times in the manifestation of the benefits of conservation practices on water quality.

Reestablishing perennial vegetation along riparian areas in agroecosystems reduces nutrient and sediment losses from agricultural lands. However, subsurface (tile) drains bypass traditional buffers routing the majority of shallow groundwater straight to surface waters, limiting their nutrient removal capabilities. Saturated riparian buffers (SRBs) reconnect subsurface drainage water with the soil profile to remove NO in tile water through microbial denitrification. One concern of enhancing denitrification on agricultural landscapes is the potential increase in NO emissions from incomplete denitrification. Our objective was to compare NO emissions from SRBs to traditional buffers and bordering crop fields at two sites, Bear Creek Site 1 and Iowa Site 1, in Central Iowa. We measured NO emissions directly from the soil surface and dissolved in shallow groundwater and estimated indirect emissions from downstream denitrification from 2015 through 2017. Nitrous oxide emissions from soil surfaces were greatest from fertilized corn ( L.). Saturated riparian buffers were only significantly greater ( < 0.05) than traditional buffers in one out of six site-years. Dissolved NO in shallow groundwater seeping from SRBs was not significantly greater ( < 0.05) than dissolved NO from the tile outlet among site years. Indirect NO emissions from rivers and estuaries were significantly reduced from NO removal in both SRBs. Overall, total NO emissions from SRBs were similar to those from traditional buffers and less than those from fertilized corn-soybean [ (L.) Merr.] agriculture. Replacing cultivated land in riparian areas with a SRB has shown potential to subsequently remove NO from surface waters and reduce NO emissions from agricultural landscapes.

Knight, Kris W., Richard C. Schultz, Cathy M. Mabry, and Thomas M. Isenhart, 2010. Ability of Remnant Riparian Forests, With and Without Grass Filters, to Buffer Concentrated Surface Runoff. Journal of the American Water Resources Association (JAWRA) 46(2):311-322. DOI: 10.1111/j.1752-1688.2010.00422.x Abstract: Riparian forest buffers established according to accepted conservation practice standards have been recommended as one of the most effective tools for mitigating nonpoint source pollution. The midwestern United States is characterized by many kilometers of narrow, naturally occurring forests along streams. However, little is known about the relative effectiveness of these remnant forests compared with these newly established buffers. This study compared the ability of naturally occurring remnant forests with and without adjacent planted grass filters to buffer concentrated flow paths (CFPs) originating in crop fields along first- and second-order streams in three northeast Missouri watersheds. Remnant forests breached by runoff through CFPs were narrower than those that dispersed 100% of the CFPs. Remnant forests with adjacent grass buffers were nearly twice the width as those without grass filters. We also found that CFPs, which developed within remnant forests and at the base of in-field grass waterways, were potential sources of sediments to streams. Methods to mitigate these CFPs warrant further investigation. Our study suggests that although these natural remnant forests provide substantial buffering capacity, both improved management and/or the addition of an adjacent grass filter would improve water quality by reducing sediment loss to streams. Inferences can be used to inform the design and management of similar conservation buffer systems within the region.

Riparian buffers can improve stream water and habitat quality by reducing non-point-source pollution (e.g., nutrients and sediment), increasing canopy cover and thereby reducing water temperature, and contributing allochthonous organic matter (e.g., leaf litter and woody debris). However, the influence of riparian buffers on biotic assemblages in streams is poorly understood, particularly in the Midwestern United States. In this study, we evaluated the effects of riparian buffers on instream habitat, fish assemblage structure, and population characteristics (i.e., the growth of two small-bodied species) in three streams in central Iowa. The streams were surveyed at two spatial scales; specifically, 41 reaches were sampled and 247 macrohabitat types (i.e., pool, riffle, and run) were subsampled in the summer of 2007. Fish assemblage structure data were summarized into separate data sets by the relative abundance of individual species (i.e., fish per minute of electrofishing) and guilds (e.g., trop...

Phosphorus and sediment are major nonpoint source pollutants that degrade water quality. Streambank erosion can contribute a significant percentage of the phosphorus and sediment load in streams. Riparian land-uses can heavily influence streambank erosion. The objective of this study was to compare streambank erosion along reaches of row-cropped fields, continuous, rotational and intensive rotational grazed pastures, pastures where cattle were fenced out of the stream, grass filters and riparian forest buffers, in three physiographic regions of Iowa. Streambank erosion was measured by surveying the extent of severely eroding banks within each riparian land-use reach and randomly establishing pin plots on subsets of those eroding banks. Based on these measurements, streambank erosion rate, erosion activity, maximum pin plot erosion rate, percentage of streambank length with severely eroding banks, and soil and phosphorus losses per unit length of stream reach were compared among the riparian land-uses. Riparian forest buffers had the lowest streambank erosion rate (15-46 mm/year) and contributed the least soil (5-18 tonne/km/year) and phosphorus (2-6 kg/km/year) to stream channels. Riparian forest buffers were followed by grass filters (erosion rates 41-106 mm/year, soil losses 22-47 tonne/km/year, phosphorus losses 9-14 kg/km/year) and pastures where cattle were fenced out of the stream (erosion rates 22-58 mm/year, soil losses 6-61 tonne/km/year, phosphorus losses 3-34 kg/km/year). The streambank erosion rates for the continuous, rotational, and intensive rotational pastures were 101-171, 104-122, and 94-170 mm/year, respectively. The soil losses for the continuous, rotational, and intensive rotational pastures were 197-264, 94-266, and 124-153 tonne/km/year, respectively, while the phosphorus losses were 71-123, 37-122, and 66 kg/km/year, respectively. The only significant differences for these pasture practices were found among the percentage of severely eroding bank lengths with intensive rotational grazed pastures having the least compared to the continuous and rotational grazed pastures. Row-cropped fields had the highest streambank erosion rates (239 mm/year) and soil losses (304 tonne/km/year) and very high phosphorus losses (108 kg/km/year).

Human alterations to the Iowa landscape, such as elimination of native vegetation for row crop agriculture and grazing, channelization of streams, and tile and ditch drainage, have led to deeply incised channels with accelerated streambank erosion. The magnitude of streambank erosion and soil loss were compared along Bear Creek in central Iowa. The subreaches are bordered by differing land uses, including reestablished riparian forest buffers, row ciop fields, and continuously grazed riparian pastures. Erosion pins were measured from June 1998 to July 2002 to estimate the magnitude of streambank erosion. Total streambank soil loss was estimated by using magnitude of bank erosion, soil bulk density, and severely eroded bank area. Significant seasonal and yearly differences in magnitude of bank erosion and total soil loss were partially attributed to differences in precipitation and associated discharges. Riparian forest buffers had significantly lower magnitude of streambank erosion and total soil loss than the other two riparian land uses. Establishment of riparian forest buffers along all of the nonbuffered subreaches would have reduced streambank soil loss by an estimated 77 to 97 percent, significantly decreasing sediment in the stream, a major water quality problem in Iowa.

An observational study was conducted at the watershed scale using land cover (vegetation) data to assess the absence or presence of riparian buffers in three northeastern Missouri watersheds. Forests and grasslands lying within a 61 m (200 ft) parallel band directly adjacent to streams were considered "buffers" for improving or protecting water quality and were characterized according to their length, width, and vegetation type. Results indicated that riparian buffers were abundant throughout the watersheds but were typically narrow along first-order and second-order streams; in many cases they may not have been wide enough to provide adequate stream protection. At least 90 percent of all streams had buffer vegetation immediately adjacent to the streambanks, but as few as 31 percent of first-order streams had buffers extending to 61 m from the stream on at least one side. On-site evaluations are needed to determine the condition of these forests and grasslands and their ability to process nonpoint source pollutants. The results will be useful for providing natural resource managers with knowledge of current watershed conditions as well as in identifying specific locations for future conservation efforts within each watershed.

Our understanding of fine-root decay processes is derived almost exclusively from litterbag studies. However, preparation of roots for litterbag studies and their sub- sequent decay within litterbags represent major departures from in situ conditions. We hypothesized that litterbag studies misrepresent fine-root decay and nutrient release rates during decomposition. To test these hypotheses we developed a new intact-core technique that requires no a priori root processing, retains natural rhizosphere associations, and main- tains in situ decay conditions. Using both litterbags and intact cores, we measured annual decay rates and nitrogen release from newly senesced fine roots of silver maple, maize, and winter wheat. After one year, mass loss was 10-23% greater, and nitrogen release was 21-29% higher within intact cores. Differences appeared to result from litterbag-induced alterations to decomposer dynamics and from unavoidable changes to fine-root size-class composition within litterbags. Our results suggest that fine-root decay and nutrient turnover occur significantly faster than estimated from litterbag studies. By minimizing disturbances to roots, soil, and rhizosphere associates prior to root decay, the intact-core technique provides an improved alternative for measuring fine-root decomposition.

A study was conducted to evaluate the ability of a multispecies riparian buffer (MRB) to remove sediment, nitrogen, and phosphorus from cropland runoff. Simulated rainfall was applied to 4.1- by 22.1-m bare cropland source areas paired with either no buffer, a 7.1-m-wide switchgrass (Panicum virgatum L. cv. Cave-n-Rock) buffer, or a 16.3-m-wide switchgrass-woody plant buffer. Each treatment plot combination had three replicates. The switchgrass buffer trapped 70% of the incoming sediment, while the switchgrass-woody buffer trapped more than 92%. In general, these buffers retained 93% of sand and silt particles and 52% of clay particles. During a 2-h rainfall simulation at 25 mm h -1 , the switchgrass buffer removed 64, 61, 72, and 44% of the incoming total N, NO 3 -N, total P, and PO 4 -P, respectively. The switchgrass-woody buffer removed 80, 92, 93, and 85% of the incoming total N, NO 3 -N, total P, and PO 4 -P, respectively. During a 1-h rainfall simulation at 69 mm h -1 , the switchgrass buffer removed 50, 41, 46, and 28% of the incoming total N, NO 3 -N, total P, and PO 4 -P, respectively. The switchgrass-woody plant buffer removed 73, 68, 81, and 35% of the incoming total N, NO 3 -N, total P, and PO 4 -P, respectively. The switchgrass buffer was effective in trapping coarse sediment and sediment-bound nutrients. But the additional buffer width with high infiltration capacity provided by the deep-rooted woody plant zone was effective in trapping the clay and soluble nutrients.

Simple and reliable procedures have been developed for analyses of N03-, total N, and organic N in freshwaters. NO,is determined by second-derivative UV spectroscopy. Total N and organic N are determined based on secondderivative analyses of NO,following persulfate digestion. Resolution of organic N determinations was increased by using ion-exchange resins to remove NO,from samples with high concentrations of N03prior to persulfate oxidation of the organic N. Although numerous methods have been developed for the determination of N in aqueous samples, there is continued need for simple and reliable procedures for both inorganic and organic N analyses. The most widely accepted methods for N03analysis include Cd reduction and ion chromatography (Am. Public Health Assoc. 1989). Both methods have been adapted for automated analysis and, with proper consideration of potential interferences, both are accurate and precise. Ion chromatography has the advantage of also measuring several additional anions in a single aliquot of samAcknowledgments This work was supported in part by the Iowa State Water Resour. Res. Inst., the Iowa DNR, the U.S. EPA, and Wetlands Res. Inc., Chicago, Illinois. We thank Iowa State University staff and researchers for their contributions to this research. Automated Cd reduction analyses were performed by the Analytical Services Laboratory (Iowa State Univ. Eng. Res. Inst.) and by the U.S. EPA ERL, Duluth, Minnesota. ple. Ion chromatography and Cd reduction, however, are relatively complicated and expensive. Samples must normally be filtered to remove suspended matter before analysis, and in the case of Cd reduction, sample color, metal ions, and phosphate are potential interferences (Olson 1980). The principal methods for organic N determination are based on conversion of organic N to NH,+ or N03and subsequent analysis of these inorganic forms. Separate analysis of ambient inorganic N allows organic N levels to be calculated by difference. For freshwater samples, conversion of organic N to inorganic forms is most commonly based on either Kjeldahl digestion (U.S. EPA 1979; Am. Public Health Assoc. 1989), photo-oxidation (Armstrong et al. 1966), or persulfate digestion (Koroleff 1976; D’Elia et al. 1977; Solorzano and Sharp 1980). Photo-oxidation requires more specialized equipment and may be ineffective for some compounds (Henriksen 1970). Kjeldahl digestion is a complicated and expensive procedure and may be unsuitable for systems receiving high inorganic N loads. High concentrations of N03severely interfere with standard Kjeldahl N determinations (Schlueter 1977; U.S. EPA 1979; Am. Public Health Assoc. 1989). N03--N concentrations 10-20 times greater than those of organic N result in >90% inhibition of Kjeldahl N determinations (Schlue