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24 results on '"Thoma R"'

23. Light it up: highly efficient multigene delivery in mammalian cells.

Author

Trowitzsch S, Klumpp M, Thoma R, Carralot JP, and Berger I

Subjects
Animals, Baculoviridae genetics, Cell Line, Gene Expression Regulation, High-Throughput Screening Assays methods, Humans, Mammals, RNA Stability, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, G-Protein-Coupled genetics, Receptors, G-Protein-Coupled metabolism, Receptors, GABA genetics, Receptors, GABA metabolism, Gene Transfer Techniques, Genetic Engineering methods, Genetic Vectors genetics
Abstract

Multigene delivery and expression systems are emerging as key technologies for many applications in contemporary biology. We have developed new methods for multigene delivery and expression in eukaryotic hosts for a variety of applications, including production of protein complexes for structural biology and drug development, provision of multicomponent protein biologics, and cell-based assays. We implemented tandem recombineering to facilitate rapid generation of multicomponent gene expression constructs for efficient transformation of mammalian cells, resulting in homogenous cell populations. Analysis of multiple parameters in living cells may require co-expression of fluorescently tagged sensors simultaneously in a single cell, at defined and ideally controlled ratios. Our method enables such applications by overcoming currently limiting challenges. Here, we review recent multigene delivery and expression strategies and their exploitation in mammalian cells. We discuss applications in drug discovery assays, interaction studies, and biologics production, which may benefit in the future from our novel approach., (Copyright © 2011 WILEY Periodicals, Inc.)

Published
2011
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24. Differentiation of diiodothyronines using electrospray ionization tandem mass spectrometry.

Author

Zhang Y, Conrad AH, Thoma R, and Conrad GW

Subjects
Diiodothyronines blood, Humans, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization, Diiodothyronines analysis, Diiodothyronines chemistry
Abstract

Diiodothyronines 3,5-diiodothyronine (3,5-T2), 3',5'-diiodothyronine (3',5'-T2), and 3,3'-diiodothyronine (3,3'-T2) are important metabolites of 3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (rT3; reverse T3). In this paper, a novel and rapid method for identifying and quantifying 3,5-T2, 3',5'-T2 and 3,3'-T2 has been introduced using electrospray ionization tandem mass spectrometry (ESI-MS/MS). Fragmentation patterns were proposed on the basis of our data obtained by ESI-MS/MS. MS2 spectra in either negative ionization mode or positive ionization mode can be used to differentiate 3,5-T2, 3',5'-T2 and 3,3'-T2. On the basis of the relative abundance of fragment ions in MS2 spectra under the positive ionization mode, quantification of the 3,5-T2, 3',5'-T2 and 3,3'-T2 isomers in mixtures is also achieved without prior separation., (Copyright 2006 John Wiley & Sons, Ltd.)

Published
2006
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