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Abstract Purpose To determine the feasibility, safety and preliminary efficacy of a telehealth supervised exercise programme in patients with advanced melanoma receiving checkpoint inhibitor therapy. Methods A 8‐week non‐randomised feasibility pilot trial utilising a telehealth delivered multimodal exercise programme undertaken thrice weekly with assessments at baseline and post‐intervention. The study was considered feasible if there were no severe or life‐threatening adverse events as a result of exercise, and three or more of the following criteria were met: the recruitment rate was >50%, completion rate was >80%, median programme attendance was >75%, median exercise compliance >75%, and average tolerance was >70%. Preliminary efficacy was assessed for objective measures of physical function (2‐min step test, repeated chair stand test, 30‐s push‐up test, and a modified static balance test) and quality of life (QoL), fatigue and other patient‐reported outcomes were assessed using the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire Core 30. Results Eleven patients (32–80 years) were included in the study (6 female, 5 male). The recruitment rate was 48%, completion rate 91%, programme attendance 88%, median exercise compliance 82.1% and 84.9% for resistance and aerobic exercise, respectively, and tolerance 88%, with no severe or life‐threatening adverse events as a result of exercise. In terms of preliminary efficacy, physical function significantly improved while QoL was maintained following the intervention. Conclusion An 8‐week telehealth exercise intervention is feasible and safe for patients with advanced melanoma and appears to improve physical function while preserving QoL during checkpoint inhibitor therapy.

Abstract Label‐free fluorescence imaging of kidney sections can provide important morphological information, but its utility has not been tested in a histology processing workflow. We tested the feasibility of label‐free imaging of paraffin‐embedded sections without deparaffinization and its potential usefulness in generating actionable data. Kidney tissue specimens were obtained during percutaneous nephrolithotomy or via diagnostic needle biopsy. Unstained non‐deparaffinized sections were imaged using widefield fluorescence microscopy to capture endogenous fluorescence. Some samples were also imaged with confocal microscopy and multiphoton excitation to collect second harmonic generation (SHG) signal to obtain high‐quality autofluorescence images with optical sectioning. To adjudicate the label‐free signal, the samples or corresponding contiguous sections were subsequently deparaffinized and stained with Lillie's allochrome. Label‐free imaging allowed the recognition of various kidney structures and enabled morphological qualification for adequacy. SHG and confocal imaging yielded quantifiable high‐quality images for tissue collagens and revealed specific patterns in glomeruli and various tubules. Disease specimens from patients with diabetic kidney disease and focal segmental glomerulosclerosis showed distinctive signatures compared to specimens from healthy controls with normal kidney function. Quantitative cytometry could also be performed when DAPI is added in situ before imaging. These results show that label‐free imaging of non‐deparaffinized sections provides useful information about tissue quality that could be beneficial to nephropathologists by maximizing the use of scarce kidney tissue. This approach also provides quantifiable features that could inform on the biology of health and disease.

Abstract Purpose Avascular necrosis of the femoral head is a common issue faced by orthopaedic surgeons that ranges between 10 and 18%, but in patients with SCD, the incidence reaches 30%. There is no definite treatment except joint arthroplasty. Regenerative medicine is an option to cure or delay joint arthroplasty. We report here our experience with the injection of ABMDO to manage ANFH and report our medium‐term results, the progression of the ANFH if any and the delay in total hip arthroplasty. (THA). Methods Sixty‐Three (63) patients with SCD and ANFH were examined and thoroughly investigated, and those who had ANFH < grade II were consented to receive ABMDO. Patients were clinically assessed preoperatively using the Visual analogue scale (VAS), Modified Harris Hips Score (MHHS) and Azam‐Sadat Score (ASS) for Quality of Life Score for Chronic Hip Disease. Ten millilitres of bone marrow were aspirated under local anaesthesia and placed in 20 CC of culture media. Osteoblasts were cultured from the aspirated bone marrow. Under anaesthesia, the osteonecrosed lesion was drilled using a 3‐mm cannulated drill, and 5 million osteoblasts were injected at the lesion site. Patients were evaluated in the outpatient clinic after 2 weeks. At 4 months, a repeat MRI was done, and patients were followed for a minimum of 2 years. Results The average age of patients was 25.93 ± 5.48 years. There were 41 (65%) females and 22 (35%) males. The mean hemoglobin S was 83.2 ± 5.1%. The average follow‐up was 49.05 ± 12.9 (range: 24–60) months. The VAS significantly improved from 7.79 ± 1.06 initially to 4.07 ± 1.08 (p

Circulating tumor DNA (ctDNA) may serve as a surrogate to tissue biopsy for noninvasive identification of mutations across multiple genetic loci and for disease monitoring in melanoma. In this study, we compared the mutation profiles of tumor biopsies and plasma ctDNA from metastatic melanoma patients using custom sequencing panels targeting 30 melanoma‐associated genes. Somatic mutations were identified in 20 of 24 melanoma biopsies, and 16 of 20 (70%) matched‐patient plasmas had detectable ctDNA. In a subgroup of seven patients for whom matching tumor tissue and plasma were sequenced, 80% of the mutations found in tumor tissue were also detected in ctDNA. However, TERT promoter mutations were only detected by ddPCR, and promoter mutations were consistently found at lower concentrations than other driver mutations in longitudinal samples. In vitro experiments revealed that mutations in promoter regions of TERT and DPH3 are underrepresented in ctDNA. While the results underscore the utility of using ctDNA as an alternative to tissue biopsy for genetic profiling and surveillance of the disease, our study highlights the underrepresentation of promoter mutations in ctDNA and its potential impact on quantitative liquid biopsy applications.

Abstract This work deals with plane strain finite element modeling for orthogonal cutting process of carbon steel. The experimental work conducted on a center lathe machine using carbide tools. The main objective was to simulate the cutting process by applying finite element method using computer software (Abaqus/CAE) to predict the cutting forces and crater wear. A comparative study was held between two modeling approaches; Lagrangian approach and Arbitrary Lagrangian‐Eulerian (ALE) approach in terms of force prediction, reading stability and chip form. The results demonstrate that ALE model is more accurate and stable than Lagrangian model, as well ALE chip form is smoother and more reliable. Johnson‐Cook (J‐C) models were utilized to define the plastic and damage properties for the tool and workpiece materials in the finite element program. Finally, this work adopted a methodology to predict crater wear experimentally by using profilometer device. On the other side, the crater wear was predicted from simulation by measuring the contact pressure on tool face. The agreement between the simulation and experimental wear results proves that the ALE model is capable to predict tool wear.

Abstract This study was carried out to develop mathematical regression equations for predicting the uptake of selected heavy metals (HMs: Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, and Zn) by Corchorus olitorius L. cultivated in soil mixed with sewage sludge based on the concentration of these HMs in the soil–sludge mixture, soil organic matter (OM), and soil pH. The resultant regression equations reflected that the three soil factors were significant predictors for the uptake of the ten HMs in the plant tissues. Applying the t test, there are no significant differences between the actual and predicted values of the ten HMs in C. olitorius root and shoot (p > .05), which reflect the best‐fitting of these equations for predicting the uptake of these HMs. Such types of equations may be helpful for evaluating the risk of cultivation due to precise prediction of C. olitorius (leafy vegetable) uptake of HMs, in soil mixed with sewage sludge.

Abstract The food security in today's world is endangered by several threats, including accelerated increases in the world's population, depletion of the Earth's resources, exaggerated environmental pollution, and foreseen climatic changes. As a strategy to diminish these threats, the wise disposal of the huge amounts of sewage sludge (SS) to agricultural soils as a bio‐fertilizer represents a worthy practice for recirculating valuable nutrients to food chains via crop plants. The current study was designed to scientifically test the feasibility of SS application to agricultural soils without causing environmental or health threats. Different SS amendment doses of 0, 10, 20, 30, 40, and 50 g/kg were supplied for soil‐grown barley (Hordeum vulgare L.) plants. Our results show that all of the SS amendment doses improved the postharvest soil properties and enhanced barley growth, with the best results obtained at the 40 g/kg dose. At this amendment dose, the organic matter (OM) content increased to 6.7% compared with 1.4% in control soil. Analysis of the postharvest soils showed that the concentrations of the investigated heavy metals (HMs) (Co, Cu, Mo, Ni, Zn, Cd, Cr, and Pb) were within the allowable limits for agricultural soils at all of the SS amendment doses, except for Co. All of the SS amendment rates enhanced barley growth and yield parameters, including shoot and root lengths and dry weights, spike weight and number of spikes per plant. Up to the 40 g/kg amendment dose, the concentrations of the investigated HMs in the harvested plant tissues were within safe limits, with most of the absorbed HMs persisting in roots. Taking these results together, the current study suggests that controlled application of SS to agricultural soils represents an eco‐friendly SS disposal procedure, which can recycle macro‐ and micronutrients to food chains and hence provide a promising approach for sustainable food security.

Abstract Conventional infrastructures based on the cloud are not sufficient for the emerging Internet of Things (IoT) applications requirements. Many big problems are shortcomings, especially in terms of network bandwidth and latency. Throughout recent years, the idea to relieve fog computing and edge computing was suggested by bringing data processing capacities closer to these limits to the edge of the network. The authors assume that the full potential of IoT will, in many cases, only be activated by the combination of cloud, fog, and edge computing in a new computing paradigm, given IoT growth and development forecasts. This article discusses the possibility and need for such a paradigm by introducing steam computing as a new distributed type of computing using cloud, fog, and edge utilities to carry out data processing and storage. The authors treat steam computing through four planes: security and privacy plane, data analytics and fault tolerance plane and deployment and test beds plane. Finally, the authors focus on the open issues and future trends in steam computing.

ABSTRACT Objectives In ovarian and other cancers, low muscle mass and density are associated with poorer clinical outcomes. However, screening for cancer‐related sarcopenia (typically defined as low muscle mass) is not routinely conducted. The European Working Group on Sarcopenia in Older People (EWGSOP) recommends an algorithm for sarcopenia screening and diagnosis in clinical settings, with sarcopenia based on muscle strength and mass, and severity on physical performance. We explored the application of the EWGSOP2 algorithm to assess sarcopenia in six ovarian cancer patients receiving neoadjuvant chemotherapy. Methods We assessed sarcopenia risk with the SARC‐F screening questionnaire (at risk ≥4 points), muscle strength with a handgrip strength test (cut point 15 s), muscle mass by skeletal muscle index (SMI in cm2/m2 from a single computed tomography [CT] image; cut point