1. Liposome-Encapsulated Hemoglobin Accelerates Skin Wound Healing in Diabetic dB/dB Mice
- Author
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Susumu Takekoshi, Tsuyoshi Fukui, Rica Tanaka, Hideaki Sumiyoshi, Muneo Miyasaka, and Akira T. Kawaguchi
- Subjects
Chemokine ,medicine.medical_specialty ,medicine.medical_treatment ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Inflammation ,02 engineering and technology ,030204 cardiovascular system & hematology ,Granulocyte ,Proinflammatory cytokine ,Biomaterials ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Saline ,biology ,business.industry ,General Medicine ,Hypoxia (medical) ,020601 biomedical engineering ,Surgery ,medicine.anatomical_structure ,biology.protein ,medicine.symptom ,business ,Wound healing ,Perfusion - Abstract
Since liposome-encapsulated hemoglobin with high O2 affinity (h-LEH, P50 O2 = 10 mm Hg) has been reported to accelerate skin wound healing in normal mice, it was tested in dB/dB mice with retarded wound healing, as seen in human diabetics. Two full-thickness dorsal wounds 6 mm in diameter encompassed by silicone stents were created in dB/dB mice. Two days later (day 2), the animals were randomly assigned to receive intravenous h-LEH (2 mL/kg, n = 7) or saline (2 mL/kg, n = 7). The same treatment was repeated 4 days after wounding (day 4), and the size of the skin lesions was analyzed by photography, surface perfusion was detected by Laser-Doppler imager, and plasma cytokines and chemokines were determined on days 0, 2, 4, and 7, when all animals were euthanized for morphological studies. The size of the ulcer compared to the skin defect or silicone stent became significantly reduced on days 4 and 7 in mice treated with h-LEH (47 ± 8% of original size), similar to the level in wild-type mice, compared to saline-treated dB/dB mice (68 ± 18%, P
- Published
- 2017