1. Mass spectrometry combined with oxidative labeling for exploring protein structure and folding.
- Author
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Konermann L, Stocks BB, Pan Y, and Tong X
- Subjects
- Amino Acids chemistry, Bacteriorhodopsins chemistry, Circular Dichroism, Deuterium, Hydrogen, Models, Molecular, Molecular Structure, Myoglobin chemistry, Oxidation-Reduction, Protein Binding, Proteins metabolism, Spectrometry, Mass, Electrospray Ionization, Hydroxyl Radical chemistry, Mass Spectrometry methods, Protein Folding, Proteins chemistry
- Abstract
This review discusses various mass spectrometry (MS)-based approaches for exploring structural aspects of proteins in solution. Electrospray ionization (ESI)-MS, in particular, has found fascinating applications in this area. For example, when used in conjunction with solution-phase hydrogen/deuterium exchange (HDX), ESI-MS is a highly sensitive tool for probing conformational dynamics. The main focus of this article is a technique that is complementary to HDX, that is, the covalent labeling of proteins by hydroxyl radicals. The reactivity of individual amino acid side chains with *OH is strongly affected by their degree of solvent exposure. Thus, analysis of the oxidative labeling pattern by peptide mapping and tandem mass spectrometry provides detailed structural information. A convenient method for *OH production is the photolysis of H(2)O(2) by a pulsed UV laser, resulting in oxidative labeling on the microsecond time scale. Selected examples demonstrate the use of this technique for structural studies on membrane proteins, and the combination with rapid mixing devices for characterizing the properties of short-lived protein (un)folding intermediates., (2009 Wiley Periodicals, Inc.)
- Published
- 2010
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