1. CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACHPeriplaneta americanaMUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE-INDUCED CURRENTS
- Author
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Steeve H. Thany and Emiliane Taillebois
- Subjects
0301 basic medicine ,medicine.medical_specialty ,animal structures ,Physiology ,Biology ,Biochemistry ,Nicotine ,03 medical and health sciences ,0302 clinical medicine ,Ganglion type nicotinic receptor ,Internal medicine ,medicine ,Acetylcholine receptor ,General Medicine ,3. Good health ,Cell biology ,Nicotinic acetylcholine receptor ,030104 developmental biology ,Endocrinology ,Nicotinic agonist ,nervous system ,Insect Science ,Mushroom bodies ,Alpha-4 beta-2 nicotinic receptor ,030217 neurology & neurosurgery ,Cys-loop receptors ,medicine.drug - Abstract
Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.
- Published
- 2016