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2. Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts

Author

Ganna V. Kalayda, Sandra Kotz, Barbara Crone, Ulrich Jaehde, Maximilian Kullmann, and Sabine Metzger

Subjects
Cisplatin, Chemotherapy, Protein Disulfide-Isomerase Family, Two-dimensional gel electrophoresis, medicine.medical_treatment, Clinical Biochemistry, Drug resistance, Biology, Pharmacology, Biochemistry, Fluorescence, Analytical Chemistry, Adduct, medicine, Intracellular, medicine.drug
Abstract

Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.

Published
2015

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