1. In vitrostudy of biological activities of anthocyanin-rich berry extracts on porcine intestinal epithelial cells
- Author
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Ruslan Mariychuk, Dana Marcinčáková, Dagmar Mudroňová, Adriana Eliašová, L’. Tkáčiková, Juraj Pistl, Ján Király, Tomáš Csank, and Petra Kšonžeková
- Subjects
chemistry.chemical_classification ,Nutrition and Dietetics ,Bilberry ,Antioxidant ,biology ,medicine.medical_treatment ,Cyanidin ,food and beverages ,Glycoside ,04 agricultural and veterinary sciences ,Berry ,Vaccinium myrtillus ,biology.organism_classification ,Sambucus nigra ,040401 food science ,chemistry.chemical_compound ,0404 agricultural biotechnology ,chemistry ,Biochemistry ,Anthocyanin ,medicine ,Food science ,Agronomy and Crop Science ,Food Science ,Biotechnology - Abstract
BACKGROUND Anthocyanins, compounds that represent the major group of flavonoids in berries, are one of the most powerful natural antioxidants. The aim of this study was to evaluate biological activities and comparison of anthocyanin-rich extracts prepared from chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), bilberry (Vaccinium myrtillus) and blueberry (V. corymbosum) on the porcine intestinal epithelial IPEC-1 cell line. RESULTS The IC50 values calculated in the antioxidant cell-based dichlorofluorescein assay (DCF assay) were 1.129 mg L−1 for chokeberry, 1.081 mg L−1 for elderberry, 2.561 mg L−1 for bilberry and 2.965 mg L−1 for blueberry, respectively. We found a significant negative correlation (P < 0.001) between cyanidin glycosides content and IC50 values. Moreover, extracts rich in cyanidin glycosides stimulated proliferation of IPEC-1 cells and did not have cytotoxic effect on cells at an equivalent in vivo concentration. CONCLUSIONS We found that the chokeberry and elderberry extracts rich in cyanidin glycosides possess better antioxidant and anticytotoxic activities in comparison to blueberry or bilberry extracts with complex anthocyanin profiles. © 2015 Society of Chemical Industry
- Published
- 2015
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