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African sharptooth catfish Clarias gariepinus were housed under continuous dim light (1lx) or 12L:12D (350-0lx) cycles. The number of skin lesions, as indicator of aggressive acts, and plasma cortisol levels, as indicator of stress-axis activity, were measured at baseline as well as following a stressor (given in the light or dark phase). Results showed that (1) baseline plasma cortisol levels were not different between photoperiods, (2) the number of baseline skin lesions was highest for C. gariepinus housed under continuous dim light, (3) stressor-induced peak levels of plasma cortisol were highest in the light phase and (4) the number of skin lesions following a stressor was highest in the dark phase. The higher number of stressor-related skin lesions in the dark (active) phase suggests increased stressor-induced aggression while in the active phase. In addition, the data suggest that housing under continuous dim light does not result in higher stress-axis activity, as measured by baseline levels of cortisol, but does result in more stressor-induced aggression, as measured by the higher number of skin lesions. The latter may be related to the fact that the continuous dim light photoperiod has twice the number of dark-phase (active) hours in which stressor-induced aggression is stronger compared to the 12L:12D photoperiod, which has a light phase in which stressor-induced aggression is lower.

Recently, we established an inhibitory avoidance paradigm in Tupfel Long-Fin (TL) zebrafish. Here, we compared task performance of TL fish and fish from the AB strain; another widely used strain and shown to differ genetically and behaviourally from TL fish. Whole-body cortisol and telencephalic gene expression related to stress, anxiety and fear were measured before and 2 h post-task. Inhibitory avoidance was assessed in a 3-day paradigm: fish learn to avoid swimming from a white to a black compartment where a 3V-shock is given: day 1 (first shock), day 2 (second shock) and day 3 (no shock, sampling). Tupfel Long-Fin fish rapidly learned to avoid the black compartment and showed an increase in avoidance-related spatial behaviour in the white compartment across days. In contrast, AB fish showed no inhibitory avoidance learning. AB fish had higher basal cortisol levels and expression levels of stress-axis related genes than TL fish. Tupfel Long-Fin fish showed post-task learning-related changes in cortisol and gene expression levels, but these responses were not seen in AB fish. We conclude that AB fish show higher cortisol levels and no inhibitory avoidance than TL fish. The differential learning responses of these Danio strains may unmask genetically defined risks for stress-related disorders.

In this study a simple modelling approach was applied to identify the need for spatial complexity in representing hydrological processes and their variability over different scales. A data set of 18 basins was used, ranging between 8 and 4011 km2 in area, located in the Nahe basin (Germany), with daily discharge values for over 30 years. Two different parsimoniously structured models were applied in lumped as well as in spatially distributed according to two distribution classifications: (1) a simple classification based on the lithology expressed in three permeability types and (2) a more complex classification based on seven dominating runoff production processes. The objective of the study was to compare the performances of the models on a local and on a regional scale as well as between the models with a view to identifying the accuracy in capturing the spatial variability of the rainfall-runoff relationships. It was shown that the presence of a specific basin characteristic or process of the distribution classification was not related with higher model performance; only a larger basin size promoted higher model performance. The results of this study also indicated that the permeability generally contained more useful information on the spatial heterogeneity of the hydrological behaviour of the natural system than did a more detailed classification on dominating runoff generation processes. Although model performance was slightly lower for the model that used permeability as a distribution classification, consistency in its parameter values was found, which was lacking with the more complex distribution classification. The latter distribution classification had a higher flexibility to optimize towards the variability of the runoff, which resulted in higher performance, however, process representation was applied inconsistently. Copyright © 2007 John Wiley & Sons, Ltd.

Here we present a newly developed tool for continuous recordings and analysis of novelty-induced and baseline behaviour of mice in a home cage-like environment. Aim of this study was to demonstrate the strength of this method by characterizing four inbred strains of mice, C57BL/6, DBA/2, C3H and 129S2/Sv, on locomotor activity. Strains differed in circadian rhythmicity, novelty-induced activity and the time-course of specific behavioural elements. For instance, C57BL/6 and DBA/2 mice showed a much faster decrease in activity over time than C3H and 129S2/Sv mice. Principal component analysis revealed two major factors within locomotor activity, which were defined as 'level of activity' and 'velocity/stops'. These factors were able to distinguish strains. Interestingly, mice that displayed high levels of activity in the initial phase of the home cage test were also highly active during an open-field test. Velocity and the number of stops during movement correlated positively with anxiety-related behaviour in the elevated plus maze. The use of an automated home cage observation system yields temporal changes in elements of locomotor activity with an advanced level of spatial resolution. Moreover, it avoids the confounding influence of human intervention and saves time-consuming human observations.

Force plate analysis was used to measure ground reaction forces (GRF) and contact times, and calculate the centre of gravity at a walk of 10 dogs which had had a limb amputated, and the results were compared with the results from 22 normal dogs of the same weight. The loss of a limb caused significant changes in the GRF, impulses and contact times of the remaining limbs and in the location of the dogs' centre of gravity. The changes were greater in dogs which had lost a forelimb than in dogs which had lost a hindlimb.

Forty-four Dutch dog owners were interviewed by telephone about their experiences with their dog after it had had a limb amputated. Most of the dogs adapted to walking on three legs within a month, sooner than most of the owners had expected. There were few complications after the amputation, although changes in behaviour were observed in approximately one-third of the dogs.

Data show that tachykinin NKA injected into the nucleus accumbens potentiated the locomotor effects of intra-accumbens d-amphetamine in habituated rats, while the spontaneous locomotor activity of habituated rats was not altered. The present results extend previous data suggesting an interaction between tachykinins and dopamine. The results point to a differential regulation of horizontal (locomotion) and vertical (rearing) movements in the nucleus accumbens of the rat consistent with previous results