1. Plant lipid-associated fibrillin proteins condition jasmonate production under photosynthetic stress
- Author
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Abir Youssef, Joël Gaffé, Yec’han Laizet, Maryse A. Block, Dominique Pontier, Jean-Pierre Alcaraz, Eric Maréchal, Tony R. Larson, Marcel Kuntz, Plastes et Différenciation Cellulaire (PDC), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), CNAP, Department of Biology, University of York [York, UK], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
0106 biological sciences ,plastoglobule ,Photoinhibition ,Arabidopsis thaliana ,Plastoglobule ,thylakoid ,Arabidopsis ,plant ,Plant Science ,01 natural sciences ,Anthocyanins ,chemistry.chemical_compound ,Jasmonate ,Photosynthesis ,plastid ,0303 health sciences ,Jasmonic acid ,Microfilament Proteins ,food and beverages ,Chloroplast ,Biochemistry ,Thylakoid ,RNA Interference ,jasmonic acid biosynthesis ,triacylglycerol ,photooxidative stress ,fibrillin ,Cyclopentanes ,Biology ,lipid associated protein ,Fibrillins ,03 medical and health sciences ,chloroplast ,Microscopy, Electron, Transmission ,Stress, Physiological ,Genetics ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Oxylipins ,Plastid ,030304 developmental biology ,Arabidopsis Proteins ,fungi ,photosystem II ,Photosystem II Protein Complex ,Cell Biology ,biology.organism_classification ,chemistry ,photosynthesis acclimation ,010606 plant biology & botany - Abstract
International audience; Summary The role of a subfamily of lipid globule-associated proteins, referred to as plant fibrillins (FIB1a, -1b, -2), was determined using a RNA interference (RNAi) strategy. We show that Arabidopsis plants with reduced levels of these plastid structural proteins are impaired in long-term acclimation to environmental constraint, namely photooxidative stress imposed by high light combined with cold. As a result, their photosynthetic apparatus is inefficiently protected. This leads to the prevalence of an abnormal granal and stromal membrane arrangement, as well as higher photosystem II photoinhibition under stress. The visible phenotype of FIB1-2 RNAi lines also includes retarded shoot growth and a deficit in anthocyanin accumulation under stress. All examined phenotypic effects of lower FIB levels are abolished by jasmonate (JA) treatment. An atypical expression pattern of several JA-induced genes was observed in RNAi plants. A JA-deficient mutant was found to share similar stress phenotypic characteristics with FIB RNAi plants. We conclude a new physiological role for JA, namely acclimation of chloroplasts, and that light/cold stress-related JA biosynthesis is conditioned by the accumulation of plastoglobule-associated FIB1-2 proteins. Consistent correlative data suggest that this FIB effect is mediated by plastoglobule (and triacylglycerol) accumulation as the potential site for initiating the chloroplast stress-related JA biosynthesis.
- Published
- 2010