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3. MAMMALS IN PORTUGAL: A data set of terrestrial, volant, and marine mammal occurrences in Portugal

Author

Grilo, Clara, primary, Afonso, Beatriz C., additional, Afonso, Filipe, additional, Alexandre, Marta, additional, Aliácar, Sara, additional, Almeida, Ana, additional, Alonso, Ivan Prego, additional, Álvares, Francisco, additional, Alves, Paulo, additional, Alves, Paulo Célio, additional, Alves, Pedro, additional, Amado, Anabela, additional, Amendoeira, Vitor, additional, Amorim, Francisco, additional, da Silva Aparício, Guilherme, additional, Araújo, Ricardo, additional, Ascensão, Fernando, additional, Augusto, Margarida, additional, Bandeira, Victor, additional, Barbosa, A. Márcia, additional, Barbosa, Soraia, additional, Barbosa, Sérgio, additional, Barreiro, Silvia, additional, Barros, Paulo, additional, Barros, Tânia, additional, Barros, Filomena, additional, Basto, Mafalda, additional, Bernardino, Joana, additional, Bicho, Sara, additional, Biedma, Luis Eduardo, additional, Borges, Marta, additional, Braz, Luis, additional, Brito, José Carlos, additional, Brito, Tiago, additional, Cabral, João Alexandre, additional, Calzada, Javier, additional, Camarinha, Cláudia, additional, Carapuço, Mafalda, additional, Cardoso, Paulo, additional, Carmo, Mário, additional, Carrapato, Carlos, additional, da Silva Carrilho, Maílis, additional, Carvalho, Diogo Filipe T. C. S., additional, Carvalho, Filipe, additional, Carvalho, João, additional, Castro, Diana, additional, Castro, Guilherme, additional, Castro, Joana, additional, Castro, Luis Roma, additional, Catry, Filipe Xavier, additional, Cerveira, Ana M., additional, Cid, André, additional, Clarke, Rafael, additional, Conde, Conceição, additional, Conde, José, additional, Costa, Jorge, additional, Costa, Mafalda, additional, Costa, Pedro, additional, Costa, Cristina, additional, do Couto, André Pedro, additional, Craveiro, João, additional, Dias, Marta, additional, Dias, Sofia, additional, Duarte, Beatriz, additional, Duro, Virginia, additional, Encarnação, Cláudia, additional, Eufrázio, Sofia, additional, Fael, António, additional, Falé, João Salvador, additional, Faria, Sandra, additional, Fernandes, Carlos, additional, Fernandes, Margarida, additional, da Costa, Gonçalo Ferrão, additional, Ferreira, Clara, additional, Ferreira, Diogo F., additional, Ferreira, Eduardo, additional, Ferreira, Joaquim Pedro, additional, Ferreira, João, additional, Ferreira, Diana, additional, Fonseca, Carlos, additional, Fontes, Inês, additional, Fragoso, Ricardo, additional, Franco, Claudia, additional, Freitas, Tamira, additional, Gabriel, Sofia I., additional, Gibb, Rory, additional, Gil, Patricia, additional, Gomes, Carla Patricia Jorge, additional, Horta, Pedro, additional, Gomes, Pedro, additional, Gomes, Verónica, additional, Grilo, Filipa, additional, Guedes, Américo, additional, Guilherme, Filipa, additional, Gutiérrez, Iván, additional, Harper, Henry, additional, Herrera, José M., additional, Hipólito, Dário, additional, Infante, Samuel, additional, Jesus, José, additional, Jones, Kate E., additional, Laborde, Marina I., additional, de Oliveira, Luís Lamas, additional, Leitão, Inês, additional, Lemos, Rita, additional, Lima, Cátia, additional, Linck, Paloma, additional, Lopes, Hugo, additional, Lopes, Susana, additional, López‐Baucells, Adrià, additional, Loureiro, Armando, additional, Loureiro, Filipa, additional, Lourenço, Rui, additional, Lourenço, Sofia, additional, Lucas, Paula, additional, Magalhães, Ana, additional, Maldonado, Cristina, additional, Marcolin, Fabio, additional, Marques, Sara, additional, Marques, J. Tiago, additional, Marques, Carina, additional, Marques, Paulo, additional, Marrecas, Pedro Caetano, additional, Martins, Frederico, additional, Martins, Raquel, additional, Mascarenhas, Miguel, additional, Mata, Vanessa A., additional, Mateus, Ana Rita, additional, Matos, Milene, additional, Medinas, Denis, additional, Mendes, Tiago, additional, Mendes, Gabriel, additional, Mestre, Frederico, additional, Milhinhas, Catarina, additional, Mira, António, additional, Monarca, Rita I., additional, Monteiro, Norberto, additional, Monteiro, Barbara, additional, Monterroso, Pedro, additional, Nakamura, Mónia, additional, Negrões, Nuno, additional, Nóbrega, Eva K., additional, Nóvoa, Miguel, additional, Nunes, Manuel, additional, Nunes, Nuno Jardim, additional, Oliveira, Flávio, additional, Oliveira, José Miguel, additional, Palmeirim, Jorge M., additional, Pargana, João, additional, Paula, Anabela, additional, Paupério, Joana, additional, Pedroso, Nuno M., additional, Pereira, Guilherme, additional, Pereira, Pedro F., additional, Pereira, José, additional, Pereira, Maria João Ramos, additional, Petrucci‐Fonseca, Francisco, additional, Pimenta, Miguel, additional, Pinto, Sara, additional, Pinto, Nuno, additional, Pires, Rosa, additional, Pita, Ricardo, additional, Pontes, Carlos, additional, Quaresma, Marisa, additional, Queirós, João, additional, Queirós, Luís, additional, Rainho, Ana, additional, da Graça Ramalhinho, Maria, additional, Ramalho, Patrícia, additional, Raposeira, Helena, additional, Rasteiro, Francisco, additional, Rebelo, Hugo, additional, Regala, Frederico Tátá, additional, Reto, Dyana, additional, Ribeiro, Sérgio Bruno, additional, Rio‐Maior, Helena, additional, Rocha, Ricardo, additional, Rocha, Rita Gomes, additional, Rodrigues, Luísa, additional, Román, Jacinto, additional, Roque, Sara, additional, Rosalino, Luís Miguel, additional, do Rosário, Inês T., additional, Rossa, Mariana, additional, Russo, Danilo, additional, Sá, Pedro, additional, Sabino‐Marques, Helena, additional, Salgueiro, Vânia, additional, Santos, Helena, additional, Santos, Joana, additional, Santos, João P. V., additional, Santos, Nuno, additional, Santos, Sara, additional, Santos, Carlos Pedro, additional, Santos‐Reis, Margarida, additional, Serronha, Ana, additional, Sierra, Pablo, additional, Silva, Bruno, additional, Silva, Carla S. G. M., additional, Silva, Clara, additional, Silva, Diogo, additional, da Silva, Luís P., additional, Silva, Ricardo, additional, Silva, Carmen, additional, da Silva Júnior, Flavio Manoel Rodrigues, additional, Sousa, Pedro, additional, Sousa‐Guedes, Diana, additional, Spadoni, Giulia, additional, Tapisso, Joaquim T., additional, Teixeira, Daniela, additional, Teixeira, Sérgio, additional, Teixeira, Nuno, additional, Torres, Rita T., additional, Travassos, Paulo, additional, Vale‐Gonçalves, Hélia, additional, Cidraes‐Vieira, Nuno, additional, von Merten, Sophie, additional, and da Luz Mathias, Maria, additional

Published
2022
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14. Evaluation of various membranes at different fluxes to enable large-volume single-use perfusion bioreactors.

Author

Raza H, Tang T, Gao B, Phuangthong C, Chen CB, and Pinto NDS

Subjects
CHO Cells, Animals, Cell Culture Techniques methods, Cell Culture Techniques instrumentation, Polymers chemistry, Sulfones chemistry, Perfusion methods, Perfusion instrumentation, Polyvinyls chemistry, Cricetinae, Fluorocarbon Polymers, Bioreactors, Membranes, Artificial, Antibodies, Monoclonal chemistry, Antibodies, Monoclonal biosynthesis, Cricetulus
Abstract

The growing demand for biological therapeutics has increased interest in large-volume perfusion bioreactors, but the operation and scalability of perfusion membranes remain a challenge. This study evaluates perfusion cell culture performance and monoclonal antibody (mAb) productivity at various membrane fluxes (1.5-5 LMH), utilizing polyvinylidene difluoride (PVDF), polyethersulfone (PES), or polysulfone (PS) membranes in tangential flow filtration mode. At low flux, culture with PVDF membrane maintained higher cell culture growth, permeate titer (1.06-1.34 g/L) and sieving coefficients (≥83%) but showed lower permeate volumetric throughput and higher transmembrane pressure (TMP) (>1.50 psi) in the later part of the run compared to cultures with PES and PS membrane. However, as permeate flux increased, the total mass of product decreased by around 30% for cultures with PVDF membrane, while it remained consistent with PES and PS membrane, and at the highest flux studied, PES membrane generated 12% more product than PVDF membrane. This highlights that membrane selection for large-volume perfusion bioreactors depends on the productivity and permeate flux required. Since operating large-volume perfusion bioreactors at low flux would require several cell retention devices and a complex setup, PVDF membranes are suitable for low-volume operations at low fluxes whereas PES membranes can be a desirable alternative for large-volume higher demand products at higher fluxes., (© 2024 Wiley Periodicals LLC.)

Published
2024
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15. Improved protein A resin for antibody capture in a continuous countercurrent tangential chromatography system.

Author

Fedorenko D, Dutta AK, Tan J, Walko J, Brower M, Pinto NDS, Zydney AL, and Shinkazh O

Subjects
Animals, CHO Cells, Cricetinae, Cricetulus, Antibodies, Monoclonal isolation & purification, Bioreactors, Countercurrent Distribution methods, Staphylococcal Protein A chemistry, Staphylococcal Protein A metabolism
Abstract

Continuous countercurrent tangential chromatography (CCTC) enables steady-state continuous bioprocessing with low-pressure operation and high productivity. CCTC has been applied to initial capture of monoclonal antibodies (mAb) from clarified cell culture harvest and postcapture polishing of mAb; however, these studies were performed with commercial chromatography resins designed for conventional column chromatography. In this study, a small particle size prototype agarose resin (20-25 µm) with lower cross-linking was co-developed with industrial partner Purolite and tested with CCTC. Due to increased binding capacity and faster kinetics, the resulting CCTC process showed more than a 2X increase in productivity, and a 2X reduction in buffer consumption over commercial protein A resins used in previous CCTC studies, as well as more than a 10X productivity increase versus conventional column operation. Single-pass tangential flow filtration was integrated with the CCTC system, enabling simple control of eluate concentration. A scale-up exercise was conducted to provide a quantitative comparison of CCTC and batch column chromatography. These results clearly demonstrate opportunities for using otherwise unpackable soft small particle size resins with CCTC as the core of a continuous bioprocessing platform., (© 2019 Wiley Periodicals, Inc.)

Published
2020
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16. Impact of micro and macroporous TFF membranes on product sieving and chromatography loading for perfusion cell culture.

Author

Pinto NDS, Napoli WN, and Brower M

Subjects
Animals, Antibodies, Monoclonal analysis, Antibodies, Monoclonal metabolism, CHO Cells, Cell Culture Techniques methods, Chromatography, Liquid, Cricetinae, Cricetulus, Equipment Design, Bioreactors, Cell Culture Techniques instrumentation, Filtration instrumentation, Membranes, Artificial
Abstract

Bioprocess intensification can be achieved through high cell density perfusion cell culture with continuous protein capture integration. Protein passage and cell retention are commonly accomplished using tangential flow filtration systems consisting of microporous membranes. Significant challenges, including low efficiency and decaying product sieving over time, are commonly observed in these cell retention devices. Here, we demonstrate that a macroporous membrane overcomes the product sieving challenges when comparing to several other membrane chemistries and pore sizes within the microporous range. This way, variable chromatography column loading is avoided. The macroporous membrane yielded a 13,000 L/m 2 volumetric throughput. The membrane's cut-off size results in an increased permeate turbidity due to particles passage, such as cell debris, through pores ranging from 1 to 4 µm. In addition, successful chromatography column plugging mitigation was achieved by employing depth filtration before the chromatographic step. Depth filtration volumetric throughputs were between 600 and 1,000 L/m 2 . Combing a macroporous cell retention device with a depth filter not only provided an alternative to address the challenge of undesired long protein residence times in the bioreactor due to product sieving decay, but also exhibited a throughput increase, making the integration of multicolumn capture chromatography with a perfusion cell culture a more robust process., (© 2019 Wiley Periodicals, Inc.)

Published
2020
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17. Co-producing knowledge of lesbian, gay, bisexual, trans and intersex (LGBTI) health-care inequalities via rapid reviews of grey literature in 27 EU Member States.

Author

Sherriff N, Zeeman L, McGlynn N, Pinto N, Hugendubel K, Mirandola M, Gios L, Davis R, Donisi V, Farinella F, Amaddeo F, Costongs C, and Browne K

Subjects
Europe, Female, Gray Literature, Health Knowledge, Attitudes, Practice, Health Services Accessibility, Humans, Male, Needs Assessment, Healthcare Disparities statistics & numerical data, Sexual and Gender Minorities psychology, Transgender Persons psychology
Abstract

Background: The health inequalities experienced by lesbian, gay, bisexual, trans and intersex (LGBTI) people are well documented with several reviews of global research summarizing key inequalities. These reviews also show how the health-care needs of LGBTI people are often poorly understood whilst suggesting that targeted initiatives to reduce inequalities should involve LGBTI people., Objectives: To determine what is known about the health-care inequalities faced by LGBTI people? What are the barriers faced by LGBTI people whilst accessing health care, and health professionals when providing care? What examples of promising practice exist?, Design: Rapid reviews of grey literature were co-produced with LGBTI people in 27 countries followed by a thematic analysis and synthesis across all data sets. The review included grey literature from each country that might not otherwise be accessible due to language barriers., Main Results: Rapid reviews showed that LGBTI people faced various inequalities and barriers whilst accessing health care. Where heterosexuality, binary gender and assumed male/female sex characteristics were upheld as the norm, and where LGBTI people differed from these norms, discrimination could result. In consultations where LGBTI people feared discrimination and did not disclose their LGBTI status, health professionals lacked the information required for appropriate assessments., Conclusion: With greater understanding of sexual orientation (LGB people), gender identity (trans people) and sex characteristics (intersex people), combined with access to contemporary knowledge and training, health professionals can work in collaboration with researchers, policymakers and LGBTI people to develop systems that are better attuned to the needs of all service users., (© 2019 The Authors. Health Expectations published by John Wiley & Sons Ltd.)

Published
2019
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18. Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co-elution of impurities.

Author

Pinto ND, Uplekar SD, Moreira AR, Rao G, and Frey DD

Subjects
Animals, Antibodies, Monoclonal chemistry, Antibodies, Monoclonal metabolism, Cell Line, Drug Contamination prevention & control, Hydrogen-Ion Concentration, Immunoglobulin G chemistry, Immunoglobulin G metabolism, Mice, Models, Molecular, Proteins chemistry, Staphylococcal Protein A chemistry, Antibodies, Monoclonal isolation & purification, Chromatography, Affinity methods, Immunoglobulin G isolation & purification, Staphylococcal Protein A metabolism
Abstract

Purification processes for monoclonal Immunoglobulin G (IgG) typically employ protein A chromatography as a capture step to remove most of the impurities. One major concern of the post-protein A chromatography processes is the co-elution of some of the host cell proteins (HCPs) with IgG in the capture step. In this work, a novel method for IgG elution in protein A chromatography that reduces the co-elution of HCPs is presented where a two-step pH gradient is self-formed inside a protein A chromatography column. The complexities involved in using an internally produced pH gradient in a protein A chromatography column employing adsorbed buffering species are discussed though equation-based modeling. Under the conditions employed, ELISA assays show a 60% reduction in the HCPs co-eluting with the IgG fraction when using the method as compared to conventional protein A elution without affecting the IgG yield. Evidence is also obtained which indicates that the amount of leached protein A present in free solution in the purified product is reduced by the new method. Biotechnol. Bioeng. 2017;114: 154-162. © 2016 Wiley Periodicals, Inc., (© 2016 Wiley Periodicals, Inc.)

Published
2017
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