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7. Automatic tracking of labeled red blood cells in microchannels.

Author

Pinho D, Lima R, Pereira AI, and Gayubo F

Subjects
Adult, Algorithms, Fluorescent Dyes chemistry, Humans, Linear Models, Microscopy, Confocal methods, Models, Biological, Cell Tracking methods, Erythrocytes cytology, Image Processing, Computer-Assisted methods, Microtechnology instrumentation
Abstract

The current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- μm glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland-Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels., (Copyright © 2012 John Wiley & Sons, Ltd.)

Published
2013
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