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Purpose: Transcutaneous oxygen tension (TcpO 2 ) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO 2 is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO 2 measurement in human subjects., Methods: We have developed a novel TcpO 2 oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen-sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3-mm diameter, 60-μm thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen-barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO 2 through the linewidth of the EPR spectrum., Results: Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO 2 measurements were robust, reliable, and reproducible. The TcpO 2 values ranged from 7.8 ± 0.8 to 22.0 ± 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 ± 0.3 to 23.4 ± 1.3 mmHg in the foot (N = 86)., Conclusions: The results demonstrated that the SPOT chip can measure TcpO 2 reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO 2 in the clinic., (© 2018 International Society for Magnetic Resonance in Medicine.)