1. Utilization of Canavalia maritima as biocatalyst for urea biosensors
- Author
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Gracliano de Oliveira Neto, Luiz M. Aleixo, Oswaldo E. S. Godinho, and Valdinete Lins da Silva
- Subjects
Chromatography ,biology ,Canavalia ,biology.organism_classification ,Analytical Chemistry ,Ammonia ,chemistry.chemical_compound ,Membrane ,chemistry ,Biocatalysis ,Electrode ,Electrochemistry ,Urea ,Glutaraldehyde ,Biosensor - Abstract
Seeds of Canavalia maritima are ground after removal of the external coat and are used for the construction of a new enzymatic electrode for urea. Aliquots (10 mg of the powder) are immobilized at the surface of an ammonia gas-sensing electrode, via glutaraldehyde or, alternatively, on PVC membranes. The analytical performance of the biosensors for serum urea determination is comparable to the spectrophotometric method.
- Published
- 1994
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