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This work explored the mechanism of augmented stress-induced vascular reactivity of senescent murine femoral arteries (FAs). Mechanical and pharmacological reactivity of young (12-25 weeks, y-FA) and senescent (>104 weeks, s-FAs) femoral arteries was measured by wire myography. Expression and protein phosphorylation of selected regulatory proteins were studied by western blotting. Expression ratio of the Exon24 in/out splice isoforms of the regulatory subunit of myosin phosphatase, MYPT1 (MYPT1-Exon24 in/out), was determined by polymerase chain reaction (PCR). While the resting length-tension relationship showed no alteration, the stretch-induced-tone increased to 8.3 ± 0.9 mN in s-FA versus only 4.6 ± 0.3 mN in y-FAs. Under basal conditions, phosphorylation of the regulatory light chain of myosin at S19 was 19.2 ± 5.8% in y-FA versus 49.2 ± 12.6% in s-FA. Inhibition of endogenous NO release raised tone additionally to 10.4 ± 1.2 mN in s-FA, whereas this treatment had a negligible effect in y-FAs (4.8 ± 0.3 mN). In s-FAs, reactivity to NO donor was augmented (pD2 = -4.5 ± 0.3 in y-FA vs. -5.2 ± 0.1 in senescent). Accordingly, in s-FAs, MYPT1-Exon24-out-mRNA, which is responsible for expression of the more sensitive to protein-kinase G, leucine-zipper-positive MYPT1 isoform, was increased. The present work provides evidence that senescent murine s-FA undergoes vascular remodelling associated with increases in stretch-activated contractility and sensitivity to NO/cGMP/PKG system.

Electromagnetic geophysical methods often rely on measurements of naturally occurring or artificially impressed electric fields. It is technically impossible, however, to measure the electric field directly. Instead, the electric field is approximated by recording the voltage difference between two electrodes and dividing the obtained voltage by the distance between the electrodes. Typically, modelling and inversion algorithms assume that the electric fields are obtained over infinitely short point‐dipoles and thus measured fields are assigned to a single point between the electrodes. Such procedures imply several assumptions: (1) The electric field between the two electrodes is regarded as constant or being a potential field and (2) the receiver dimensions are negligible compared to the dimensions of the underlying modelling grid. While these conditions are often fulfilled for horizontal electric fields, borehole sensors for recordings of the vertical electric field have dimensions in the order of ≈100 m and span several modelling grid cells. Observations from such elongated borehole sensors can therefore only be interpreted properly if true receiver dimensions and variations of electrical conductivity along the receiver are considered. Here, we introduce a numerical solution to include the true receiver geometry into electromagnetic modelling schemes, which does not rely on such simplifying assumptions. The algorithm is flexible, independent of the chosen numerical method to solve Maxwell's equations and can easily be implemented in other electromagnetic modelling and inversion codes. We present conceptual modelling results for land‐based controlled source electromagnetic scenarios and discuss consideration of true receiver geometries for a series of examples of horizontal and vertical electric field measurements. Comparison with Ez data measured in an observation borehole in a producing oil field shows the importance of both considering the true length of the receiver and also its orientation. We show that misalignment from the vertical axis as small as 0.1° may seriously distort the measured signal, as horizontal electric field components are mapped into the desired vertical component. Adequate inclusion of elongated receivers in modelling and inversion can also help reducing effects of static shift when interpreting (natural source) magnetotelluric data.

Objectives Low emotional intelligence (EI) may predispose individuals to applying maladaptive coping strategies. This may maintain anxious worrying, which is highly prevalent in patients with chronic heart failure (CHF) and may affect mental (MCS) and physical component summaries (PCS) of health-related quality of life (HRQoL). Design The current study is a cross-sectional and cross-cultural survey. Methods N = 200 outpatients with CHF were recruited at cardiology institutes in Germany and Pakistan and assessed with self-report questionnaires. Results Path analysis (χ2 (4) = 7.59, p = .11, GFI = .99) revealed that the expected associations between low EI and lower SF-36 MCS and PCS of HRQoL were fully mediated by negative metacognition and maladaptive coping in the Pakistani sample (p's ≤ .05). The German sample applied different maladaptive coping strategies, which also led to lower MCS and PCS scores, but did not mediate a direct positive effect of EI on HRQoL. Conclusion The current findings support culture-independent validity of the metacognitive model but also reveal major cultural differences regarding the application and effect of specific maladaptive coping strategies. This has important implications for caregivers in a cross-cultural context and highlights the need for culture-specific tailoring of psychosocial interventions. Statement of contribution What is already known on this subject? Worry, an integral component of generalized anxiety disorder (GAD) and highly comorbid in chronic heart failure (CHF) patients, contributes to anxiety and resulting stress as evident from metacognitive model of GAD. In addition, previous literature has also established the protective role of emotional intelligence (EI) against stress, thus maintaining quality of life. What does this study add? Cross-cultural (Pakistan vs. Germany) validation of the metacognitive model of GAD. Supportive evidence for the metacognitive model in patients with CHF. Mediation of maladaptive metacognitions and negative coping in the relationship of low trait EI and low health-related quality of life.

Objective: Cardiac diseases are established risk factors for ischemic stroke incidence and severity. Conversely, there is increasing evidence that brain ischemia can cause cardiac dysfunction. The mechanisms underlying this neurogenic heart disease are incompletely understood. While it is established that ischemic stroke is associated with cardiac arrhythmias, myocardial damage, elevated cardiac enzymes and plasma catecholamines in the acute phase, nothing is known about the delayed consequences of ischemic stroke on cardiovascular function. Methods: To determine the long-term cardiac consequences of a focal cerebral ischemia, we subjected young and aged mice to a 30-minute transient middle cerebral artery occlusion and analyzed cardiac function by serial transthoracic echocardiography and hemodynamic measurements up to week 8 after surgery. Finally, animals were treated with metoprolol to evaluate a pharmacologic treatment option to prevent the development of heart failure. Results: Focal cerebral ischemia induced a long-term cardiac dysfunction with a reduction in left ventricular ejection fraction and an increase in left ventricular volumes; this development was associated with higher peripheral sympathetic activity. Metoprolol treatment prevented the development of chronic cardiac dysfunction by decelerating extracellular cardiac remodeling and inhibiting sympathetic signaling relevant to chronic autonomic dysfunction. Interpretation: Focal cerebral ischemia in mice leads to the development of chronic systolic dysfunction driven by increased sympathetic activity. If these results can be confirmed in a clinical setting, treating physicians should be attentive to clinical signs of heart failure in every patient after ischemic stroke. Therapeutically, the successful β-blockade with metoprolol in mice could also have future clinical implications. This article is protected by copyright. All rights reserved.

Thrombus-in-transit crossing an interatrial communication is a rare but potentially serious clinical condition, which has so far not been described after implantation of left atrial appendage (LAA) closure devices. Here, we describe the case of a 76-year-old woman with permanent atrial fibrillation and contraindication for oral anticoagulation therapy, who developed pericardial tamponade and acute pulmonary embolism with visible thrombus-in-transit formation following LAA closure. Most likely manipulation of the Watchman device in the LAA during two failed attempts in device positioning led to pericardial tamponade and venous puncture, manual compression after completion of the procedure and further immobilization resulted in deep vein thrombosis with consecutive pulmonary embolism and thrombus-in-transit formation. This case highlights the value of echocardiography after LAA closure.

Electromagnetic methods are routinely applied to image the subsurface from shallow to regional structures. Individual electromagnetic methods differ in their sensitivities towards resistive and conductive structures and in their exploration depths. If a good balance between different electromagnetic data can be be found, joint 3D inversion of multiple electromagnetic datasets can result in significantly better resolution of subsurface structures than the individual inversions.We present a weighting algorithm to combine magnetotelluric, controlled source electromagnetic, and geoelectric data. Magnetotelluric data are generally more sensitive to regional conductive structures, whereas controlled source electromagnetic and geoelectric data are better suited to recover more shallow and resistive structures. Our new scheme is based on weighting individual components of the total data gradient after each model update. Norms of individual data residuals are used to assess how much of the total data gradient must be assigned to each method to achieve a balanced contribution of all datasets for the joint inverse model. Synthetic inversion tests demonstrate advantages of joint inversion in general and also the influence of the weighting. In our tests, the controlled source electromagnetic data gradients are larger than those of the magnetotelluric and geoelectric datasets. Consequently, direct joint inversion of controlled source electromagnetic, magnetotelluric, and geoelectric data results in models that are mostly dominated by structures required by the controlled source electromagnetic data. Applying the new adaptive weighting scheme results in an inversion model that fits the data better and resembles more the original model. We used the modular system electromagnetic as a framework to implement the new joint inversion and briefly describe the new modules for forward modelling and their interfaces to the modular system electromagnetic package.

We carried out a magnetotelluric field campaign in the South–East Lower Saxony Basin, Germany, with the main goal of testing this method for imaging regional Posidonia black shale sediments. Two-dimensional inversion results of the magnetotelluric data show a series of conductive structures correlating with brine-saturated sediments but also with deeper, anthracitic Westphalian/Namurian coals. None of these structures can be directly related with the Posidonia black shale, which appears to be generally resistive and therefore difficult to resolve with the magnetotelluric method. This assumption is supported by measurements of electrical resistivity on a set of Posidonia shale samples from the Hils syncline in the Lower Saxony basin. These rock samples were collected in shallow boreholes and show immature (0.53% Ro), oil (0.88% Ro), and gas (1.45% Ro) window thermal maturities. None of the black shale samples showed low electrical resistivity, particularly those with oil window maturity show resistivity exceeding 104 m. Moreover, we could not observe a direct correlation between maturity and electrical resistivity; the Harderode samples showed the highest resistivity, whereas the Haddessen samples showed the lowest. A similar trend has been seen for coals in different states of thermal maturation. Saturation of the samples with distilled and saline water solutions led to decreasing electrical resistivity. Moreover, a positive correlation of electrical resistivity with porosity is observed for the Wickensen and Harderode samples, which suggests that the electrical resistivity of the Posidonia black shale is mainly controlled by porosity.

To advance and optimize secondary and tertiary oil recovery techniques, it is essential to know the areal propagation and distribution of the injected fluids in the subsurface. We investigate the applicability of controlled-source electromagnetic methods to monitor fluid movements in a German oilfield (Bockstedt, onshore Northwest Germany) as injected brines (highly saline formation water) have much lower electrical resistivity than the oil within the reservoir. The main focus of this study is on controlled-source electromagnetic simulations to test the sensitivity of various source–receiver configurations. The background model for the simulations is based on two-dimensional inversion of magnetotelluric data gathered across the oil field and calibrated with resistivity logs. Three-dimensional modelling results suggest that controlled-source electromagnetic methods are sensitive to resistivity changes at reservoir depths, but the effect is difficult to resolve with surface measurements only. Resolution increases significantly if sensors or transmitters can be placed in observation wells closer to the reservoir. In particular, observation of the vertical electric field component in shallow boreholes and/or use of source configurations consisting of combinations of vertical and horizontal dipoles are promising. Preliminary results from a borehole-to-surface controlled-source electromagnetic field survey carried out in spring 2014 are in good agreement with the modelling studies.

The basic physical properties of the magnetic source field, namely its homogeneity and spatial coherence, have been used for a variety of magnetotelluric processing techniques including remote reference processing. In the present work we propose a data acquisition and processing technique for a large number of stations distributed over a localized area ideally on a grid. For pseudo-remote reference processing it is necessary to use the following station setup: five-component MT data are only measured at some sites (base stations) while at the majority of sites (local stations) only the electric and vertical magnetic fields are recorded. The impedance tensor and vertical magnetic transfer functions at each local station are computed by assigning the magnetic fields of a base station to the local station as if they had been measured there. This approach can lead to biased or erroneous estimates of local transfer functions at stations in the vicinity of strong conductivity contrasts that can be corrected using the interstation transfer functions between the horizontal magnetic fields measured at the base station(s). We test this approach with a data set collected in the vicinity of the Gros Schonebeck geothermal test site. Magnetotelluric data were collected at 146 local and 5 base stations distributed over an approximately 5 km × 25 km wide grid with site spacing ranging from 500 m × 500 m to 1000 m × 1000 m in the frequency range 128–0.001Hz. The obtained pseudo-remote reference transfer functions are generally smooth and consistent and conductivity models obtained from 2D inversion of the data are in agreement with previous conductivity models from the study area.

There are serious concerns regarding safety when performing magnetic resonance imaging in patients with implanted conductive medical devices, such as cardiac pacemakers, and associated leads, as severe incidents have occurred in the past. In this study, several approaches for altering an implant's lead design were systematically developed and evaluated to enhance the safety of implanted medical devices in a magnetic resonance imaging environment. The individual impact of each design change on radiofrequency heating was then systematically investigated in functional lead prototypes at 1.5 T. Radiofrequency-induced heating could be successfully reduced by three basic changes in conventional pacemaker lead design: (1) increasing the lead tip area, (2) increasing the lead conductor resistance, and (3) increasing outer lead insulation conductivity. The findings show that radiofrequency energy pickup in magnetic resonance imaging can be reduced and, therefore, patient safety can be improved with dedicated construction changes according to a "safe by design" strategy. Incorporation of the described alterations into implantable medical devices such as pacemaker leads can be used to help achieve favorable risk-benefit-ratios when performing magnetic resonance imaging in the respective patient group.

Terra Nova, 24, 207–212, 2012 Abstract The subsurface structure of the Cape Fold Belt (CFB) that traverses southern Africa remains largely unknown because it lacks deep-penetrating, high-resolution geophysics. This hampers studies from local-scale exploration for important resources such as water, to continental-scale modelling, such as Gondwana reconstructions. Our Magnetotelluric (MT) soundings across the CFB provide a high-resolution crustal-scale electrical conductivity image. Inversion models of these data correlate well with surface geology, and resolve deep resistive roots of the CFB’s regional Palaeozoic quartzite ridges (Swartberg and Outeniqua Mountains) and thin tectonic wedges of Proterozoic rocks of higher resistivity (Kango and Kaaimans Inliers), separated by 2–3 km of Cretaceous sediments in asymmetric intermontaine basins. One of these basins, the Oudtshoorn Basin, is flanked by a listric normal fault rooted in an upper crustal detachment zone, and is underlain by a massive conductivity anomaly that is likely to be a major saline water reservoir.

Determining the MR compatibility of medical implants and devices is becoming increasingly relevant. In most cases, the heating of conductive implants due to radiefrequency (RF) excitation pulses is measured by fluoroptic temperature sensors in relevant tests for approval. Another common method to determine these heating effects is MR thermometry using the proton resonance frequency. This method gives good results in homogeneous phantoms. However in many cases, technical shortcomings such as susceptibility artifacts prohibit exact proton resonance frequency thermometry near medical implants. Therefore, this work aimed at developing a fast T₁-based method which allows controlled MR-related heating of a medical implant while simultaneously quantifying the spatial and temporal temperature distribution. To this end, an inversion recovery snapshot Fast Low-Angle Shot (FLASH) sequence was modified with additional off-resonant heating pulses. With an accelerated imaging method and a sliding-window technique, every 7.6 s a new temperature map could be generated with a spatial in-plane resolution of 2 mm. The temperature deviation from calculated temperature values to reference fluoroptic probe was found to be smaller than 1 K.

Implanted medical devices such as cardiac pacemakers pose a potential hazard in magnetic resonance imaging. Electromagnetic fields have been shown to cause severe radio frequency-induced tissue heating in some cases. Imaging exclusion zones have been proposed as an instrument to reduce patient risk. The purpose of this study was to further assess the impact of the imaging landmark on the risk for unintended implant heating by measuring the radio frequency-induced electric fields in a body phantom under several imaging conditions at 1.5T. The results show that global radio frequency-induced coupling is highest with the torso centered along the superior-inferior direction of the transmit coil. The induced E-fields inside the body shift when changing body positioning, reducing both global and local radio frequency coupling if body and/or conductive implant are moved out from the transmit coil center along the z-direction. Adequate selection of magnetic resonance imaging landmark can significantly reduce potential hazards in patients with implanted medical devices.

The basic helix-loop-helix transcription factors eHAND and dHAND are involved in developmental cardiac growth and differentiation. We investigated HAND gene expression in the normal and in the hypertrophied right and left ventricle of patients with tetralogy of Fallot (ToF) and hypertrophic obstructive cardiomyopathy (HOCM). HAND mRNA was constitutively expressed in the hypertrophied heart and increased in the hypertrophic tissue of both patient groups. HAND genes had a complementary left-right cardiac asymmetry of expression with dHAND predominantly in the right and eHAND in the left ventricle. The two cardiac bHLH factors have the ability to form heterodimers with the ubiquitous bHLH protein E12, subsequently recognizing E-boxes in the promoter region of target genes like ALC-1. We found a highly significant positive correlation between HAND and ALC-1 mRNA. The total ALC-1 protein level in ToF was smaller than in HOCM, although ALC-1 mRNA as well as HAND mRNA levels were significantly higher. ToF patients expressed around four times more ALC-1 mRNA for similar amounts of ALC-1 than HOCM patients. Suggesting disturbed ALC-1 translation in ToF, we found ALC-1 antisense mRNA expression in the hypertrophied, but not in the normal, ventricles. The higher the antisense/sense ALC-1 mRNA ratio, the lower ALC-1 protein was expressed. J. Cell. Biochem. 74:551–561, 1999. © 1999 Wiley-Liss, Inc.