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Soil moisture information is essential to monitoring of the intensity of droughts, the start of the rainy season, planting dates and early warnings of yield losses. We assess spatial and temporal trends of drought over the Brazilian semiarid region by combining soil moisture observations from 360 stations, root zone soil moisture from a leading land surface model, and a vegetation health index from remote sensing. The soil moisture dataset was obtained from the network of stations maintained by the National Center of Monitoring and Early Warning of Natural Disasters (Cemaden), in Brazil. Soil water content at 10 to 35 cm depth, for the period 1979–2018, was obtained from running the JULES land surface model (the Joint UK Land Environment Simulator). The modelled soil moisture was correlated with measurements in the common period of 2015–2018, resulting in an average correlation coefficient of 0.48 across the domain. The standardized soil moisture anomaly (SMA) was calculated for the long-term modelled soil moisture and revealed strong negative values during well-known drought periods in the region, especially during El-Nino years. The performance of SMA in identifying droughts during the first 2 months of the raining and cropping season was similar to the Standardized Precipitation Index (SPI), commonly used for drought assessment: 12–14 events were identified by both indices. Finally, the temporal relationship between both SMA and SPI with the Vegetation Health Index (VHI) was assessed using the cross-wavelet transform. The results indicated lagged correlations of 1 to 1.5 months in the annual scale, suggesting that negative trends in SMA and SPI can be an early warning to yield losses during the growing season. Public policies on drought assessment should consider the combination of multiple drought indices, including soil moisture anomaly.

Background and objectives With disease-modifying treatment strategies on the horizon, stratification of individual patients at the earliest stages of Parkinson's disease (PD) is key-ideally already at clinical disease onset. Blood levels of neurofilament light chain (NfL) provide an easily accessible fluid biomarker that might allow capturing the conversion from prodromal to manifest PD. Methods We assessed longitudinal serum NfL levels in subjects converting from prodromal to manifest sporadic PD (converters), at-risk subjects, and matched controls (72 participants with ≈4 visits), using single-molecule array (Simoa) technique. Results While NfL levels were not increased at the prodromal stage, subjects converting to the manifest motor stage showed a significant intraindividual acceleration of the age-dependent increase of NfL levels. Conclusions The temporal dynamics of intraindividual NfL blood levels might mark the conversion to clinically manifest PD, providing a potential stratification biomarker for individual disease onset in the advent of precision medicine for PD. © 2020 The Authors. Movement Disorders published by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society.

SUMMARY Nitric oxide (NO) is a cell-signaling molecule that regulates a variety of molecular pathways. We investigated the role of NO during preimplantation embryonic development by blocking its production with an inhibitor or supplementing in vitro bovine embryo cultures with its natural precursor, L-arginine, over different periods. Endpoints evaluated included blastocyst rates, development kinetics, and embryo quality. Supplementation with the NO synthase inhibitor N-Nitro-L-arginine-methyl ester (L-NAME) from Days 1 to 8 of culture decreased blastocyst (P

Chronic Hepatitis B (HB) is the main risk factor for chronic liver disease (CLD) and hepatocellular carcinoma (HCC) in many low-resource countries, where diagnosis is constrained by lack of clinical, histopathological and biomarker resources. We have used proteomics to detect plasma biomarkers that outperform α-Fetoprotein (AFP), the most widely used biomarker for HCC diagnosis in low-resource contexts. Deep-plasma proteome analysis was performed in HCC patients, patients with CLD and in HB-carrier controls from Thailand (South-East Asia) and The Gambia (West-Africa). Mass spectrometry profiling identified latent-transforming growth factor β binding-protein 2 (LTBP2) and Osteopontin (OPN) as being significantly elevated in HCC versus CLD and controls. These two proteins were further analyzed by ELISA in a total of 684 plasma samples, including 183 HCC, 274 CLD and 227 asymptomatic controls. When combined, LTBP2 and OPN showed an area under the receiver operating curve of 0.85 in distinguishing HCC from CLD in subjects with AFP

Priapism is defined as prolonged and persistent penile erection, unassociated with sexual interest or stimulation and increase the development of erectile dysfunction(ED), and is a complication associated with sickle cell disease (SCD). Moreover, prevalence of ED associated with priapism increases progressively with aging, but, understanding of stages involved is still poorly investigated. Thus, we evaluate the muscle reactivity of isolated corpus cavernosum (CC) of SCD old mice. The animals were divided in control (CT; 3 month), control old (CTO; 1.2 year) and SCD old (SSO; 1.2 year) groups. Concentration-response curves to acethylcholine (ACh), sodium nitroprusside (SNP) and phenylephrine (PE) were obtained in CC mice. Nitrergic and neurogenic responses induced by electrical-field stimulation (EFS) were also obtained in all groups. The potency (pEC50) to ACh did not change in all group, but, the maximal response (Emax) was decrease in SSO (Emax: 20 ± 9%, P